Restriction map for
pFN21AB8640
(Circular) (Six-base) MAPSORT of: pFN21AB8640.seq Check: 9686 from: 1 to: 5314
pFN21AB8640 5314 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2190 3124 278
Size: 53 83 186 936 654 934 2468
Fragments arranged by size:
2468 936 934 654 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 2280 2657 1937
Size: 223 120 377 4594
Fragments arranged by size:
4594 377 223 120
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 3441 3814 1563
Size: 1878 373 3063
Fragments arranged by size:
3063 1878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4761 5029 1632
Size: 1745 1384 268 1917
Fragments arranged by size:
1917 1745 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 4213 5190 1203
Size: 3010 977 1327
Fragments arranged by size:
3010 1327 977
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AlwNI CAG_nnn'CTG
Cuts at: 2410 2457 3057 4629 2410
Size: 47 600 1572 3095
Fragments arranged by size:
3095 1572 600 47
ApaLI G'TGCA_C
Cuts at: 3371 4527 3371
Size: 1156 4158
ApoI r'AATT_y
Cuts at: 1493 2623 2849 3001 5110 1493
Size: 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 226 152
AscI GG'CGCG_CC
Cuts at: 2358 2358
Size: 5314
AseI AT'TA_AT
Cuts at: 160 1032 3868 160
Size: 872 2836 1606
Fragments arranged by size:
2836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2093 2203 2210 2474 2639 1870
Size: 73 98 52 110 7 264 165 4545
Fragments arranged by size:
4545 264 165 110 98 73 52 7
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2153 2635 4090 618
Size: 325 217 627 249 117 482 1455 1842
Fragments arranged by size:
1842 1455 627 482 325 249 217 117
BanII G_rGCy'C
Cuts at: 1391 2210 2633 1391
Size: 819 423 4072
Fragments arranged by size:
4072 819 423
BbeI G_GCGC'C
Cuts at: 2040 2157 2040
Size: 117 5197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5309 5309
Size: 5314
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 3375 4531 1790
Size: 1585 1156 2573
Fragments arranged by size:
2573 1585 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2266 3974 1239
Size: 1027 1708 2579
Fragments arranged by size:
2579 1708 1027
Bpu10I CC'TnA_GC
Cuts at: 2238 2238
Size: 5314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 3439 4304 4602 2188
Size: 1251 865 298 2900
Fragments arranged by size:
2900 1251 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 3977 882
Size: 617 837 1641 2219
Fragments arranged by size:
2219 1641 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 2503 3105 493
Size: 711 1110 189 602 2702
Fragments arranged by size:
2702 1110 711 602 189
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2154 2187 2265
Size: 53 83 186 936 504 117 33 78
Cuts at: 2265 3121 3503 4138 275
Size: 856 382 635 1451
Fragments arranged by size:
1451 936 856 635 504 382 186 117
83 78 53 33
BsaWI w'CCGG_w
Cuts at: 2218 3747 4145 4419 4566 4977 2218
Size: 1529 398 274 147 411 2555
Fragments arranged by size:
2555 1529 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5089
Fragments arranged by size:
5089 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 1186
Size: 1051 4263
BseYI C'CCAG_C
Cuts at: 4517 5013 4517
Size: 496 4818
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2541 1163
Size: 573 805 3936
Fragments arranged by size:
3936 805 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2483 3375 3460 4531 2277
Size: 206 892 85 1071 3060
Fragments arranged by size:
3060 1071 892 206 85
BsmI GAATG_Cn'
Cuts at: 2825 2918 2825
Size: 93 5221
BsmBI CGTCTCn'nnnn_
Cuts at: 2516 2516
Size: 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2277 2483 2633 3375 3460 4531
Size: 399 420 67 206 150 742 85 1071
Cuts at: 4531 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 420 399 206 150 85
67
BspEI T'CCGG_A
Cuts at: 2218 4977 2218
Size: 2759 2555
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 844
Size: 1825 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2350 3958 4145 1700
Size: 110 293 247 1608 187 2869
Fragments arranged by size:
2869 1608 293 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5314
BssHII G'CGCG_C
Cuts at: 1925 2358 2428 2430 2545 2547 2549 2597 1925
Size: 433 70 2 115 2 2 48 4642
Fragments arranged by size:
4642 433 115 70 48 2 2 2
BssSI C'ACGA_G
Cuts at: 2378 3374 4386 2378
Size: 996 1012 3306
Fragments arranged by size:
3306 1012 996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2410 2506 794
Size: 1616 96 3602
Fragments arranged by size:
3602 1616 96
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2380
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2644 3011 3396 3413 4865 4951 4963 5309
Size: 672 367 385 17 1452 86 12 346
Cuts at: 5309 1972
Size: 1977
Fragments arranged by size:
1977 1452 672 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 2136 2423 513
Size: 552 939 105 27 287 3404
Fragments arranged by size:
3404 939 552 287 105 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 487
Size: 683 56 1094 3481
Fragments arranged by size:
3481 1094 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2156 2186 4321 809
Size: 507 840 30 2135 1802
Fragments arranged by size:
2135 1802 840 507 30
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2058 2228
Size: 54 1425 50 275 18 195 33 170
Cuts at: 2228 2436 2761 3650 5279 8
Size: 208 325 889 1629 43
Fragments arranged by size:
1629 1425 889 325 275 208 195 170
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2519 3244 1353
Size: 1166 725 3423
Fragments arranged by size:
3423 1166 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 2473 3901 4275 4421 387
Size: 1064 138 468 416 1428 374 146 1280
Fragments arranged by size:
1428 1280 1064 468 416 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2266 3377 3974 4761 5029 1239
Size: 393 634 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 634 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2320 2320
Size: 5314
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
FspI TGC'GCA
Cuts at: 2450 3820 2450
Size: 1370 3944
HaeII r_GCGC'y
Cuts at: 1678 2040 2101 2157 2372 2464 2588 4461 1678
Size: 362 61 56 215 92 124 1873 2531
Fragments arranged by size:
2531 1873 362 215 124 92 61 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3955 3987 4029 4061 1373
Size: 32 135 32 2383 32 42 32 2626
Fragments arranged by size:
2626 2383 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KasI G'GCGC_C
Cuts at: 2036 2153 2036
Size: 117 5197
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4428 4612 1305
Size: 3123 184 2007
Fragments arranged by size:
3123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2230 10
Size: 54 1425 343 398 3094
Fragments arranged by size:
3094 1425 398 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3272 3631 3790 518
Size: 648 1212 894 359 159 2042
Fragments arranged by size:
2042 1212 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2407 2425 2730 3407 4555 4800 1606
Size: 532 269 18 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 532 305 269 245 18
NaeI GCC'GGC
Cuts at: 2352 2352
Size: 5314
NarI GG'CG_CC
Cuts at: 2037 2154 2037
Size: 117 5197
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NgoMIV G'CCGG_C
Cuts at: 2350 2350
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2672 4217 1132
Size: 97 1443 1545 2229
Fragments arranged by size:
2229 1545 1443 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 4213 4213
Size: 5314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PmlI CAC'GTG
Cuts at: 2503 2503
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PspXI vC'TCGA_Gb
Cuts at: 2203 2474 2203
Size: 271 5043
PstI C_TGCA'G
Cuts at: 830 2294 2580 2606 2666 830
Size: 1464 286 26 60 3478
Fragments arranged by size:
3478 1464 286 60 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
RsrII CG'GwC_CG
Cuts at: 2420 2420
Size: 5314
SacII CC_GC'GG
Cuts at: 2139 2426 2139
Size: 287 5027
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2580 2666 2580
Size: 86 5228
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SfcI C'TryA_G
Cuts at: 826 1045 2290 2576 2602 2662 3797 4478 4669
Size: 219 1245 286 26 60 1135 681 191
Cuts at: 4669 826
Size: 1471
Fragments arranged by size:
1471 1245 1135 681 286 219 191 60
26
SfoI GGC'GCC
Cuts at: 2038 2155 2038
Size: 117 5197
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2043 2212 2641 1872
Size: 171 169 429 4545
Fragments arranged by size:
4545 429 171 169
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 2203 2474 3418 4319 4581
Size: 197 926 224 36 271 944 901 262
Cuts at: 4581 820
Size: 1553
Fragments arranged by size:
1553 944 926 901 271 262 224 197
36
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 3238 5
Size: 47 3186 2081
Fragments arranged by size:
3186 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2753 513
Size: 552 939 749 3074
Fragments arranged by size:
3074 939 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3341 3358 3511 3696 4141 325
Size: 1255 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 3560 5272
Size: 274 80 33 51 1443 513 1070 1712
Cuts at: 5272 96
Size: 138
Fragments arranged by size:
1712 1443 1070 513 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5274 5306 5274
Size: 32 5282
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 2203 2474 1943
Size: 260 271 4783
Fragments arranged by size:
4783 271 260
XmaI C'CCGG_G
Cuts at: 1870 2041 2210 2639 1870
Size: 171 169 429 4545
Fragments arranged by size:
4545 429 171 169
XmnI GAAnn'nnTTC
Cuts at: 3443 3443
Size: 5314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2188 3122 276
Size: 53 83 186 936 654 934 2468
Fragments arranged by size:
2468 936 934 654 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PspOMI PsrI PvuII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI