Restriction map for
pFN21AB8632
(Circular) (Six-base) MAPSORT of: pFN21AB8632.seq Check: 5909 from: 1 to: 7219
pFN21AB8632 7219 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5029 278
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4562 1937
Size: 2625 4594
Acc65I G'GTAC_C
Cuts at: 4540 4540
Size: 7219
AclI AA'CG_TT
Cuts at: 1563 5346 5719 1563
Size: 3783 373 3063
Fragments arranged by size:
3783 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 3015 3198 3354 3620 3810 5282 6666
Size: 801 582 183 156 266 190 1472 1384
Cuts at: 6666 6934 1632
Size: 268 1917
Fragments arranged by size:
1917 1472 1384 801 582 268 266 190
183 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7022
AflIII A'CryG_T
Cuts at: 1203 6118 7095 1203
Size: 4915 977 1327
Fragments arranged by size:
4915 1327 977
AgeI A'CCGG_T
Cuts at: 3687 6050 3687
Size: 2363 4856
AhdI GACnn_n'nnGTC
Cuts at: 5948 5948
Size: 7219
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7219
AlwNI CAG_nnn'CTG
Cuts at: 3242 3740 4962 6534 3242
Size: 498 1222 1572 3927
Fragments arranged by size:
3927 1572 1222 498
ApaI G_GGCC'C
Cuts at: 2395 3103 2395
Size: 708 6511
ApaLI G'TGCA_C
Cuts at: 5276 6432 5276
Size: 1156 6063
ApoI r'AATT_y
Cuts at: 1493 2402 3494 3549 3702 3803 3825 4234 4445
Size: 909 1092 55 153 101 22 409 211
Cuts at: 4445 4528 4754 4906 7015 1493
Size: 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 909 409 226 211 153
152 101 83 55 22
AseI AT'TA_AT
Cuts at: 160 1032 3815 5773 160
Size: 872 2783 1958 1606
Fragments arranged by size:
2783 1958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
AvaI C'yCGr_G
Cuts at: 1870 1943 3894 4544 1870
Size: 73 1951 650 4545
Fragments arranged by size:
4545 1951 650 73
BamHI G'GATC_C
Cuts at: 3263 3832 4549 4916 3263
Size: 569 717 367 5566
Fragments arranged by size:
5566 717 569 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2558 4540 5995 618
Size: 325 217 627 771 1982 1455 1842
Fragments arranged by size:
1982 1842 1455 771 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2395 3103 4538 1391
Size: 1004 708 1435 4072
Fragments arranged by size:
4072 1435 1004 708
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2697 928
Size: 971 798 5450
Fragments arranged by size:
5450 971 798
BbvCI CC'TCA_GC
Cuts at: 3279 3441 3279
Size: 162 7057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5410 5444 1903
Size: 34 3473 34 3678
Fragments arranged by size:
3678 3473 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5111 6321 5111
Size: 1210 6009
BclI T'GATC_A
Cuts at: 1548 3643 1548
Size: 2095 5124
BfrBI ATG'CAT
Cuts at: 3455 3455
Size: 7219
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5830 136
Size: 107 122 71 631 605 4158 1525
Fragments arranged by size:
4158 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7214 7214
Size: 7219
BlpI GC'TnA_GC
Cuts at: 4461 4636 4461
Size: 175 7044
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2395 3103 5280 6436 1790
Size: 245 360 708 2177 1156 2573
Fragments arranged by size:
2573 2177 1156 708 360 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 2151 3283
Size: 416 53 83 399 24 602 132 1132
Cuts at: 3283 4950 5908 442
Size: 1667 958 1753
Fragments arranged by size:
1753 1667 1132 958 602 416 399 132
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 7219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 2739 3051 3445 5879 1239
Size: 1140 360 312 394 2434 2579
Fragments arranged by size:
2579 2434 1140 394 360 312
Bpu10I CC'TnA_GC
Cuts at: 2957 3279 3441 2957
Size: 322 162 6735
Fragments arranged by size:
6735 322 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 3596 4106 4439 5344 6209 6507 2259
Size: 1337 510 333 905 865 298 2971
Fragments arranged by size:
2971 1337 905 865 510 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 2684 4307 5882 882
Size: 617 657 528 1623 1575 2219
Fragments arranged by size:
2219 1623 1575 657 617 528
BsaAI yAC'GTr
Cuts at: 493 1204 5010 493
Size: 711 3806 2702
Fragments arranged by size:
3806 2702 711
BsaBI GATnn'nnATC
Cuts at: 7213 7213
Size: 7219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5026 5408 6043 275
Size: 53 83 186 936 3493 382 635 1451
Fragments arranged by size:
3493 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3687 5652 6050 6324 6471 6882 3687
Size: 1965 398 274 147 411 4024
Fragments arranged by size:
4024 1965 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 1178
Size: 30 165 30 1461 30 5503
Fragments arranged by size:
5503 1461 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 2666 2948 3164 3260 3458 4466 1186
Size: 1432 48 282 216 96 198 1008 3939
Fragments arranged by size:
3939 1432 1008 282 216 198 96 48
BseYI C'CCAG_C
Cuts at: 2057 2075 6422 6918 2057
Size: 18 4347 496 2358
Fragments arranged by size:
4347 2358 496 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3753 1163
Size: 573 2017 4629
Fragments arranged by size:
4629 2017 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4669 5430 6458 1540
Size: 275 186 2668 761 1028 2301
Fragments arranged by size:
2668 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5280 5365 6436 5280
Size: 85 1071 6063
Fragments arranged by size:
6063 1071 85
BsmI GAATG_Cn'
Cuts at: 4730 4823 4730
Size: 93 7126
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2395 3103 4538 5280 5365 6436
Size: 399 245 360 708 1435 742 85 1071
Cuts at: 6436 1391
Size: 2174
Fragments arranged by size:
2174 1435 1071 742 708 399 360 245
85
BspEI T'CCGG_A
Cuts at: 6882 6882
Size: 7219
BspHI T'CATG_A
Cuts at: 3844 5108 6838 3844
Size: 1264 1730 4225
Fragments arranged by size:
4225 1730 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4574 844
Size: 3730 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5106 1394
Size: 429 3283 3507
Fragments arranged by size:
3507 3283 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 3870 5714 5888 65
Size: 1183 966 1656 1844 174 1396
Fragments arranged by size:
1844 1656 1396 1183 966 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3687 5863 6050 1700
Size: 110 1877 2176 187 2869
Fragments arranged by size:
2869 2176 1877 187 110
BsrGI T'GTAC_A
Cuts at: 96 3408 96
Size: 3312 3907
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7219
BssSI C'ACGA_G
Cuts at: 5279 6291 5279
Size: 1012 6207
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7219
BstBI TT'CG_AA
Cuts at: 6023 6023
Size: 7219
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7219
BstXI CCAn_nnnn'nTGG
Cuts at: 3595 3595
Size: 7219
BstYI r'GATC_y
Cuts at: 1972 2816 2852 3073 3263 3832 4549 4916 5301
Size: 844 36 221 190 569 717 367 385
Cuts at: 5301 5318 6770 6856 6868 7214 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 844 717 569 385 367 346
221 190 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 4500 4500
Size: 7219
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6480
Fragments arranged by size:
6480 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 2472 4735 5529 5549 2424
Size: 48 2263 794 20 4094
Fragments arranged by size:
4094 2263 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4278 4313 529
Size: 35 3714 35 3435
Fragments arranged by size:
3714 3435 35 35
DraI TTT'AAA
Cuts at: 4523 4523
Size: 7219
DraIII CAC_nnn'GTG
Cuts at: 4073 4073
Size: 7219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6226 809
Size: 507 4910 1802
Fragments arranged by size:
4910 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2462 2469 3306
Size: 54 1425 50 275 18 632 7 837
Cuts at: 3306 4666 5555 7184 8
Size: 1360 889 1629 43
Fragments arranged by size:
1629 1425 1360 889 837 632 275 54
50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 4666 1537
Size: 275 2854 4090
Fragments arranged by size:
4090 2854 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2718 2748 3030 3309 3333 3552 3612
Size: 705 660 30 282 279 24 219 60
Cuts at: 3612 3786 4311 5149 1353
Size: 174 525 838 3423
Fragments arranged by size:
3423 838 705 660 525 282 279 219
174 60 30 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3276 5806 6180 6326 387
Size: 1064 138 1687 2530 374 146 1280
Fragments arranged by size:
2530 1687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2433 2739 3015 3051 3198 3354
Size: 393 747 54 306 276 36 147 156
Cuts at: 3354 3445 3620 3810 5282 5879 6666 6934 1239
Size: 91 175 190 1472 597 787 268 1524
Fragments arranged by size:
1524 1472 787 747 597 393 306 276
268 190 175 156 147 91 54 36
EcoO109I rG'GnC_Cy
Cuts at: 2127 2506 3099 3168 3354 3465 2127
Size: 379 593 69 186 111 5881
Fragments arranged by size:
5881 593 379 186 111 69
EcoRI G'AATT_C
Cuts at: 3494 3549 3803 4528 4906 3494
Size: 55 254 725 378 5807
Fragments arranged by size:
5807 725 378 254 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3824 4411 3824
Size: 587 6632
FspI TGC'GCA
Cuts at: 5725 5725
Size: 7219
HaeII r_GCGC'y
Cuts at: 1678 2328 6366 1678
Size: 650 4038 2531
Fragments arranged by size:
4038 2531 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2471 2503 2514
Size: 32 135 32 581 32 286 32 11
Cuts at: 2514 2546 2678 2710 2831 2863 3272 3304 3611
Size: 32 132 32 121 32 409 32 307
Cuts at: 3611 3643 3734 3766 5860 5892 5934 5966 1373
Size: 32 91 32 2094 32 42 32 2626
Fragments arranged by size:
2626 2094 581 409 307 286 135 132
121 91 42 32 32 32 32 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3115 4563 4809 669
Size: 988 281 1177 1448 246 3079
Fragments arranged by size:
3079 1448 1177 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7219
HpaI GTT'AAC
Cuts at: 4809 4809
Size: 7219
KpnI G_GTAC'C
Cuts at: 4544 4544
Size: 7219
MfeI C'AATT_G
Cuts at: 4818 4818
Size: 7219
MluI A'CGCG_T
Cuts at: 7095 7095
Size: 7219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2872 6333 6517 1305
Size: 1567 3461 184 2007
Fragments arranged by size:
3461 2007 1567 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2464 2471 3308 10
Size: 54 1425 343 632 7 837 3921
Fragments arranged by size:
3921 1425 837 632 343 54 7
MslI CAynn'nnrTG
Cuts at: 518 1166 4039 5177 5536 5695 518
Size: 648 2873 1138 359 159 2042
Fragments arranged by size:
2873 2042 1138 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3440 3759 4093 4635 5312 6460 6705 1606
Size: 1834 319 334 542 677 1148 245 2120
Fragments arranged by size:
2120 1834 1148 677 542 334 319 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6667
NdeI CA'TA_TG
Cuts at: 387 4040 387
Size: 3653 3566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7219
NotI GC'GGCC_GC
Cuts at: 4666 4666
Size: 7219
NsiI A_TGCA'T
Cuts at: 3457 3457
Size: 7219
NspI r_CATG'y
Cuts at: 1132 1229 4577 6122 1132
Size: 97 3348 1545 2229
Fragments arranged by size:
3348 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2563 2640 2748 1348
Size: 1215 77 108 5819
Fragments arranged by size:
5819 1215 108 77
PciI A'CATG_T
Cuts at: 6118 6118
Size: 7219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 6082
PfoI T'CCnGG_A
Cuts at: 3696 3696
Size: 7219
PmeI GTTT'AAAC
Cuts at: 4523 4523
Size: 7219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2689 5288 5320 6801 6833 2657
Size: 32 2599 32 1481 32 3043
Fragments arranged by size:
3043 2599 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2127 3168 3354 2127
Size: 1041 186 5992
Fragments arranged by size:
5992 1041 186
PshAI GACnn'nnGTC
Cuts at: 1613 2732 1613
Size: 1119 6100
PsiI TTA'TAA
Cuts at: 4376 4789 4376
Size: 413 6806
PspOMI G'GGCC_C
Cuts at: 2391 3099 2391
Size: 708 6511
PstI C_TGCA'G
Cuts at: 830 3758 4571 830
Size: 2928 813 3478
Fragments arranged by size:
3478 2928 813
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7219
PvuII CAG'CTG
Cuts at: 3440 3759 4093 3440
Size: 319 334 6566
Fragments arranged by size:
6566 334 319
SalI G'TCGA_C
Cuts at: 1936 4561 1936
Size: 2625 4594
SapI GCTCTTCn'nnn_
Cuts at: 3612 3612
Size: 7219
SbfI CC_TGCA'GG
Cuts at: 4571 4571
Size: 7219
ScaI AGT'ACT
Cuts at: 5467 7179 5467
Size: 1712 5507
SexAI A'CCwGG_T
Cuts at: 2765 3502 2765
Size: 737 6482
SfcI C'TryA_G
Cuts at: 826 1045 2697 3754 4567 5702 6383 6574 826
Size: 219 1652 1057 813 1135 681 191 1471
Fragments arranged by size:
1652 1471 1135 1057 813 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7219
SmaI CCC'GGG
Cuts at: 1872 4546 1872
Size: 2674 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 3575 4085 4418 5323 6224
Size: 197 926 331 1301 510 333 905 901
Cuts at: 6224 6486 820
Size: 262 1553
Fragments arranged by size:
1553 1301 926 905 901 510 333 331
262 197
SnaBI TAC'GTA
Cuts at: 493 5010 493
Size: 4517 2702
SpeI A'CTAG_T
Cuts at: 152 2527 152
Size: 2375 4844
SphI G_CATG'C
Cuts at: 1132 4577 1132
Size: 3445 3774
SspI AAT'ATT
Cuts at: 5 52 4293 4333 5143 5
Size: 47 4241 40 810 2081
Fragments arranged by size:
4241 2081 810 47 40
StuI AGG'CCT
Cuts at: 1517 2657 3380 1517
Size: 1140 723 5356
Fragments arranged by size:
5356 1140 723
StyI C'CwwG_G
Cuts at: 513 1065 2118 2346 2370 4658 513
Size: 552 1053 228 24 2288 3074
Fragments arranged by size:
3074 2288 1053 552 228 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 2576 5246 5263 5416 5601 6046
Size: 1255 657 339 2670 17 153 185 445
Cuts at: 6046 325
Size: 1498
Fragments arranged by size:
2670 1498 1255 657 445 339 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3408 5465 7177
Size: 274 80 33 51 1443 1431 2057 1712
Cuts at: 7177 96
Size: 138
Fragments arranged by size:
2057 1712 1443 1431 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7179 7211 7179
Size: 32 7187
Tth111I GACn'n_nGTC
Cuts at: 1316 2167 3482 1316
Size: 851 1315 5053
Fragments arranged by size:
5053 1315 851
XbaI T'CTAG_A
Cuts at: 3959 4555 3959
Size: 596 6623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2210 2246 2402 2588 2883 2138
Size: 72 36 156 186 295 6474
Fragments arranged by size:
6474 295 186 156 72 36
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7219
XmaI C'CCGG_G
Cuts at: 1870 4544 1870
Size: 2674 4545
XmnI GAAnn'nnTTC
Cuts at: 2550 3880 5348 2550
Size: 1330 1468 4421
Fragments arranged by size:
4421 1468 1330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5027 276
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI