Restriction map for
pFN21AB8631
(Circular) (Six-base) MAPSORT of: pFN21AB8631.seq Check: 4041 from: 1 to: 6097
pFN21AB8631 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3385 3385
Size: 6097
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 2925 3118 3440 1937
Size: 556 432 193 322 4594
Fragments arranged by size:
4594 556 432 322 193
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2607 2658 2940 3032 4160 5544 5812
Size: 516 459 51 282 92 1128 1384 268
Cuts at: 5812 1632
Size: 1917
Fragments arranged by size:
1917 1384 1128 516 459 282 268 92
51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 2612 1166
Size: 1446 4651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2155 3193 1184
Size: 971 1038 4088
Fragments arranged by size:
4088 1038 971
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2643 3406 3632 3784 5893 1493
Size: 1150 763 226 152 2109 1697
Fragments arranged by size:
2109 1697 1150 763 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 3422 1870
Size: 73 238 1241 4545
Fragments arranged by size:
4545 1241 238 73
BamHI G'GATC_C
Cuts at: 2230 3427 3794 2230
Size: 1197 367 4533
Fragments arranged by size:
4533 1197 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3418 4873 618
Size: 325 217 627 1631 1455 1842
Fragments arranged by size:
1842 1631 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 2218 2391 3416 1391
Size: 703 124 173 1025 4072
Fragments arranged by size:
4072 1025 703 173 124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 4927
Fragments arranged by size:
4927 971 199
BbvCI CC'TCA_GC
Cuts at: 3312 3312
Size: 6097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 4158 5314 1790
Size: 2368 1156 2573
Fragments arranged by size:
2573 2368 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 3828 4786
Size: 416 53 83 399 24 1158 1253 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1253 1158 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 4757 1239
Size: 1092 2426 2579
Fragments arranged by size:
2579 2426 1092
Bpu10I CC'TnA_GC
Cuts at: 3312 3312
Size: 6097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 3076 4222 5087 5385 2492
Size: 584 1146 865 298 3204
Fragments arranged by size:
3204 1146 865 584 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2783 4760 882
Size: 617 504 780 1977 2219
Fragments arranged by size:
2219 1977 780 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3103 3888 493
Size: 711 1899 785 2702
Fragments arranged by size:
2702 1899 785 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2076 2106 2275 2305 3060
Size: 30 165 30 673 30 169 30 755
Cuts at: 3060 3090 1178
Size: 30 4185
Fragments arranged by size:
4185 755 673 169 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 1186
Size: 1378 4719
BseYI C'CCAG_C
Cuts at: 2055 2076 2221 5300 5796 2055
Size: 21 145 3079 496 2356
Fragments arranged by size:
3079 2356 496 145 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 1163
Size: 573 768 4756
Fragments arranged by size:
4756 768 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4158 4243 5314 4158
Size: 85 1071 4941
Fragments arranged by size:
4941 1071 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2218 2391 3416 4158 4243 5314
Size: 399 304 124 173 1025 742 85 1071
Cuts at: 5314 1391
Size: 2174
Fragments arranged by size:
2174 1071 1025 742 399 304 173 124
85
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 3385 3452 844
Size: 1682 859 67 3489
Fragments arranged by size:
3489 1682 859 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3984 1394
Size: 429 2161 3507
Fragments arranged by size:
3507 2161 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 4592 4766 65
Size: 1183 891 2453 174 1396
Fragments arranged by size:
2453 1396 1183 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2498 4741 4928 1700
Size: 110 202 486 2243 187 2869
Fragments arranged by size:
2869 2243 486 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2527 794
Size: 1733 4364
BstBI TT'CG_AA
Cuts at: 2894 4901 2894
Size: 2007 4090
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2039 2230 3130 3427 3794 4179 4196 5648
Size: 67 191 900 297 367 385 17 1452
Cuts at: 5648 5734 5746 6092 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 900 385 367 346 297 191
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2657 2657
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 4492
Fragments arranged by size:
4492 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 3613 4407 4427 2137
Size: 1476 794 20 3807
Fragments arranged by size:
3807 1476 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2378 2933 5104 809
Size: 507 1062 555 2171 1802
Fragments arranged by size:
2171 1802 1062 555 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2293 3212 3544
Size: 54 1425 50 275 18 463 919 332
Cuts at: 3544 4433 6062 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 919 889 463 332 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2898 4027 1353
Size: 705 840 1129 3423
Fragments arranged by size:
3423 1129 840 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2331 2607 2658 2940 3032 4160
Size: 393 516 183 276 51 282 92 1128
Cuts at: 4160 4757 5544 5812 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1128 787 597 516 393 282 276
268 183 92 51
EcoO109I rG'GnC_Cy
Cuts at: 2186 2286 2186
Size: 100 5997
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3308 3308
Size: 6097
FspI TGC'GCA
Cuts at: 2732 4603 2732
Size: 1871 4226
FspAI rTGC'GCAy
Cuts at: 2732 2732
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2435 2467 2782
Size: 32 135 32 504 32 327 32 315
Cuts at: 2782 2814 2899 2931 3060 3092 4738 4770 4812
Size: 32 85 32 129 32 1646 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 1646 504 327 315 135 129 85
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2809 3441 3687 669
Size: 988 281 871 632 246 3079
Fragments arranged by size:
3079 988 871 632 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2295 3214 10
Size: 54 1425 343 463 919 2893
Fragments arranged by size:
2893 1425 919 463 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2612 4055 4414 4573 518
Size: 648 1248 198 1443 359 159 2042
Fragments arranged by size:
2042 1443 1248 648 359 198 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2221 2351 2448 3513 4190 5338 5583
Size: 470 145 130 97 1065 677 1148 245
Cuts at: 5583 1606
Size: 2120
Fragments arranged by size:
2120 1148 1065 677 470 245 145 130
97
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2731 3455 5000 1132
Size: 97 1502 724 1545 2229
Fragments arranged by size:
2229 1545 1502 724 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2286 2186
Size: 100 5997
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2144 2862 3169 3382 3449 830
Size: 1314 718 307 213 67 3478
Fragments arranged by size:
3478 1314 718 307 213 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2076 2351 2448 2076
Size: 275 97 5725
Fragments arranged by size:
5725 275 97
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 3053 4345 6057 3053
Size: 1292 1712 3093
Fragments arranged by size:
3093 1712 1292
SfcI C'TryA_G
Cuts at: 826 1045 2140 2172 2858 3165 3378 3445 4580
Size: 219 1095 32 686 307 213 67 1135
Cuts at: 4580 5261 5452 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1095 686 681 307 219 213
191 67 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 3055 4201 5102 5364 820
Size: 197 926 564 548 1146 901 262 1553
Fragments arranged by size:
1553 1146 926 901 564 548 262 197
SnaBI TAC'GTA
Cuts at: 493 3103 3888 493
Size: 2610 785 2702
Fragments arranged by size:
2702 2610 785
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 2731 3455 1132
Size: 1599 724 3774
Fragments arranged by size:
3774 1599 724
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 3536 513
Size: 552 939 1532 3074
Fragments arranged by size:
3074 1532 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 4124 4141 4294 4479 4924 325
Size: 1255 863 1681 17 153 185 445 1498
Fragments arranged by size:
1681 1498 1255 863 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 3051 3333
Size: 274 80 33 51 1443 300 774 282
Cuts at: 3333 4343 6055 96
Size: 1010 1712 138
Fragments arranged by size:
1712 1443 1010 774 300 282 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 6057 6089 2275
Size: 32 3750 32 2283
Fragments arranged by size:
3750 2283 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2218
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI