Restriction map for
pFN21AB8629
(Circular) (Six-base) MAPSORT of: pFN21AB8629.seq Check: 8583 from: 1 to: 6298
pFN21AB8629 6298 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3303 3641 1937
Size: 1366 338 4594
Fragments arranged by size:
4594 1366 338
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 3597 4425 4798 1563
Size: 2034 828 373 3063
Fragments arranged by size:
3063 2034 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 3140 4361 5745 6013 1632
Size: 1472 36 1221 1384 268 1917
Fragments arranged by size:
1917 1472 1384 1221 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 2260 2892 2922 2928 2997 5197 6174 1203
Size: 1057 632 30 6 69 2200 977 1327
Fragments arranged by size:
2200 1327 1057 977 632 69 30 6
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2247
Size: 32 6266
AlwNI CAG_nnn'CTG
Cuts at: 3110 4041 5613 3110
Size: 931 1572 3795
Fragments arranged by size:
3795 1572 931
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3092 3470 3557 3607 3833 3985 6094 1493
Size: 1599 378 87 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1599 378 226 152 87 50
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 3623 1870
Size: 73 1680 4545
Fragments arranged by size:
4545 1680 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2178 2211 2178
Size: 33 6265
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 3033 3619 5074 618
Size: 325 217 627 300 946 586 1455 1842
Fragments arranged by size:
1842 1455 946 627 586 325 300 217
BanII G_rGCy'C
Cuts at: 1391 3617 1391
Size: 2226 4072
BbeI G_GCGC'C
Cuts at: 3037 3037
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 3063 3225 3323 928
Size: 971 175 989 162 98 3903
Fragments arranged by size:
3903 989 971 175 162 98
BbvCI CC'TCA_GC
Cuts at: 2092 2092
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 2382 1548
Size: 834 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4909 136
Size: 107 122 71 631 605 3237 1525
Fragments arranged by size:
3237 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6293 6293
Size: 6298
BlpI GC'TnA_GC
Cuts at: 2825 3715 2825
Size: 890 5408
Bme1580I G_kGCm'C
Cuts at: 1790 2922 4359 5515 1790
Size: 1132 1437 1156 2573
Fragments arranged by size:
2573 1437 1156 1132
BmgBI CAC'GTC
Cuts at: 1318 2895 2998 1318
Size: 1577 103 4618
Fragments arranged by size:
4618 1577 103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3254 4029 4987
Size: 416 53 83 399 24 1837 775 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
1837 1753 958 775 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 3263 4958 1239
Size: 954 1070 1695 2579
Fragments arranged by size:
2579 1695 1070 954
Bpu10I CC'TnA_GC
Cuts at: 2092 2092
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2034 4423 5288 5586 2034
Size: 2389 865 298 2746
Fragments arranged by size:
2746 2389 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4961 882
Size: 617 3462 2219
Fragments arranged by size:
3462 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2923 4089 493
Size: 711 1719 1166 2702
Fragments arranged by size:
2702 1719 1166 711
BsaBI GATnn'nnATC
Cuts at: 3239 6292 3239
Size: 3053 3245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3034 4105 4487 5122
Size: 53 83 186 936 1501 1071 382 635
Cuts at: 5122 275
Size: 1451
Fragments arranged by size:
1501 1451 1071 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4731 5129 5403 5550 5961 4731
Size: 398 274 147 411 5068
Fragments arranged by size:
5068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6073
Fragments arranged by size:
6073 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 1186
Size: 1319 4979
BseYI C'CCAG_C
Cuts at: 2616 2641 2797 2830 5501 5997 2616
Size: 25 156 33 2671 496 2917
Fragments arranged by size:
2917 2671 496 156 33 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2971 1163
Size: 573 1235 4490
Fragments arranged by size:
4490 1235 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4359 4444 5515 4359
Size: 85 1071 5142
Fragments arranged by size:
5142 1071 85
BsmI GAATG_Cn'
Cuts at: 3421 3809 3902 3421
Size: 388 93 5817
Fragments arranged by size:
5817 388 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3031 3031
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2922 3617 4359 4444 5515 1391
Size: 399 1132 695 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 742 695 399 85
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2829 3315 3653 844
Size: 1985 486 338 3489
Fragments arranged by size:
3489 1985 486 338
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2559 3561 4793 4967 65
Size: 1183 1311 1002 1232 174 1396
Fragments arranged by size:
1396 1311 1232 1183 1002 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 96
Size: 2829 3469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 3028 4358 5370 3028
Size: 1330 1012 3956
Fragments arranged by size:
3956 1330 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6298
BstBI TT'CG_AA
Cuts at: 3127 5102 3127
Size: 1975 4323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 3628 3995 4380 4397 5849 5935 5947 6293
Size: 1656 367 385 17 1452 86 12 346
Cuts at: 6293 1972
Size: 1977
Fragments arranged by size:
1977 1656 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2583 513
Size: 552 939 579 4228
Fragments arranged by size:
4228 939 579 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2335 487
Size: 683 56 1109 4450
Fragments arranged by size:
4450 1109 683 56
BtsI GCAGTG_nn'
Cuts at: 3814 4608 4628 3814
Size: 794 20 5484
Fragments arranged by size:
5484 794 20
ClaI AT'CG_AT
Cuts at: 1554 3243 1554
Size: 1689 4609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DraIII CAC_nnn'GTG
Cuts at: 2701 2701
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2476 3745 4634
Size: 54 1425 50 275 18 646 1269 889
Cuts at: 4634 6263 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1269 889 646 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4228 1353
Size: 2875 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4885 5259 5405 387
Size: 1064 138 3296 374 146 1280
Fragments arranged by size:
3296 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 3104 3140 3263 4361 4958 5745
Size: 393 561 911 36 123 1098 597 787
Cuts at: 5745 6013 1239
Size: 268 1524
Fragments arranged by size:
1524 1098 911 787 597 561 393 268
123 36
EcoRI G'AATT_C
Cuts at: 3470 3557 3607 3985 3470
Size: 87 50 378 5783
Fragments arranged by size:
5783 378 87 50
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 3037 5445 1678
Size: 1359 2408 2531
Fragments arranged by size:
2531 2408 1359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 4939 4971 5013
Size: 32 135 32 473 32 2862 32 42
Cuts at: 5013 5045 1373
Size: 32 2626
Fragments arranged by size:
2862 2626 473 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3304 3642 3888 669
Size: 988 281 1366 338 246 3079
Fragments arranged by size:
3079 1366 988 338 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 3033 3033
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1996 5412 5596 1305
Size: 691 3416 184 2007
Fragments arranged by size:
3416 2007 691 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2478 10
Size: 54 1425 343 646 3830
Fragments arranged by size:
3830 1425 646 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 4256 4615 4774 518
Size: 648 1677 1413 359 159 2042
Fragments arranged by size:
2042 1677 1413 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2438 2641 2668 2797 3714 4391 5539 5784
Size: 832 203 27 129 917 677 1148 245
Cuts at: 5784 1606
Size: 2120
Fragments arranged by size:
2120 1148 917 832 677 245 203 129
27
NarI GG'CG_CC
Cuts at: 3034 3034
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2004 2583 513
Size: 552 939 579 4228
Fragments arranged by size:
4228 939 579 552
NdeI CA'TA_TG
Cuts at: 387 2788 387
Size: 2401 3897
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 2264 2932 3656 5201 1132
Size: 97 1035 668 724 1545 2229
Fragments arranged by size:
2229 1545 1035 724 668 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 2260 2928 5197 2260
Size: 668 2269 3361
Fragments arranged by size:
3361 2269 668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6298
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PmlI CAC'GTG
Cuts at: 2923 2923
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 2466 3868 2466
Size: 1402 4896
PstI C_TGCA'G
Cuts at: 830 2902 3442 3523 3650 830
Size: 2072 540 81 127 3478
Fragments arranged by size:
3478 2072 540 127 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2438 2641 2668 2797 2438
Size: 203 27 129 5939
Fragments arranged by size:
5939 203 129 27
SalI G'TCGA_C
Cuts at: 1936 3302 3640 1936
Size: 1366 338 4594
Fragments arranged by size:
4594 1366 338
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SfcI C'TryA_G
Cuts at: 826 1045 2152 2496 2898 3324 3438 3519 3646
Size: 219 1107 344 402 426 114 81 127
Cuts at: 3646 4781 5462 5653 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 681 426 402 344 219
191 127 114 81
SfoI GGC'GCC
Cuts at: 3035 3035
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2049 4402 5303 5565 820
Size: 197 926 106 2353 901 262 1553
Fragments arranged by size:
2353 1553 926 901 262 197 106
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2004 2344 2583 3737 513
Size: 552 939 340 239 1154 3074
Fragments arranged by size:
3074 1154 939 552 340 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 4325 4342 4495 4680 5125 325
Size: 1255 1410 1335 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1335 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2186 2925 4544
Size: 274 80 33 51 1443 209 739 1619
Cuts at: 4544 6256 96
Size: 1712 138
Fragments arranged by size:
1712 1619 1443 739 274 209 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 3393 3425 6258 6290 2062
Size: 32 1299 32 2833 32 2070
Fragments arranged by size:
2833 2070 1299 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3299 1316
Size: 1983 4315
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2590 2327
Size: 263 6035
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 4427 4427
Size: 6298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI