Restriction map for
pFN21AB8628
(Circular) (Six-base) MAPSORT of: pFN21AB8628.seq Check: 3789 from: 1 to: 7228
pFN21AB8628 7228 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5038 278
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2773 2782 4571 1937
Size: 836 9 1789 4594
Fragments arranged by size:
4594 1789 836 9
Acc65I G'GTAC_C
Cuts at: 4549 4549
Size: 7228
AclI AA'CG_TT
Cuts at: 1563 5355 5728 1563
Size: 3792 373 3063
Fragments arranged by size:
3792 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2826 3956 4286 4415 5291 6675 6943
Size: 995 199 1130 330 129 876 1384 268
Cuts at: 6943 1632
Size: 1917
Fragments arranged by size:
1917 1384 1130 995 876 330 268 199
129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7031
AflIII A'CryG_T
Cuts at: 1203 6127 7104 1203
Size: 4924 977 1327
Fragments arranged by size:
4924 1327 977
AgeI A'CCGG_T
Cuts at: 6059 6059
Size: 7228
AhdI GACnn_n'nnGTC
Cuts at: 5957 5957
Size: 7228
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7228
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3355 3387 3355
Size: 32 7196
AlwNI CAG_nnn'CTG
Cuts at: 4309 4971 6543 4309
Size: 662 1572 4994
Fragments arranged by size:
4994 1572 662
ApaLI G'TGCA_C
Cuts at: 5285 6441 5285
Size: 1156 6072
ApoI r'AATT_y
Cuts at: 1493 2379 2420 3693 4238 4537 4763 4915 7024
Size: 886 41 1273 545 299 226 152 2109
Cuts at: 7024 1493
Size: 1697
Fragments arranged by size:
2109 1697 1273 886 545 299 226 152
41
AseI AT'TA_AT
Cuts at: 160 1032 4412 5782 160
Size: 872 3380 1370 1606
Fragments arranged by size:
3380 1606 1370 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 4553 1870
Size: 73 1707 903 4545
Fragments arranged by size:
4545 1707 903 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3590 3623 3590
Size: 33 7195
BamHI G'GATC_C
Cuts at: 2836 4558 4925 2836
Size: 1722 367 5139
Fragments arranged by size:
5139 1722 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4549 6004 618
Size: 325 217 627 2762 1455 1842
Fragments arranged by size:
2762 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4058 4384 4547 1391
Size: 2667 326 163 4072
Fragments arranged by size:
4072 2667 326 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 928
Size: 971 620 5637
Fragments arranged by size:
5637 971 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5419 5453 1903
Size: 34 3482 34 3678
Fragments arranged by size:
3678 3482 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2386 3591 5120 6330 2386
Size: 1205 1529 1210 3284
Fragments arranged by size:
3284 1529 1210 1205
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7228
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5839 136
Size: 107 122 71 631 605 4167 1525
Fragments arranged by size:
4167 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2569 3073 7223 2569
Size: 504 4150 2574
Fragments arranged by size:
4150 2574 504
BlpI GC'TnA_GC
Cuts at: 4344 4645 4344
Size: 301 6927
Bme1580I G_kGCm'C
Cuts at: 1790 5289 6445 1790
Size: 3499 1156 2573
Fragments arranged by size:
3499 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2907 4424 1318
Size: 1589 1517 4122
Fragments arranged by size:
4122 1589 1517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4319 4959 5917
Size: 416 53 83 399 24 2902 640 958
Cuts at: 5917 442
Size: 1753
Fragments arranged by size:
2902 1753 958 640 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2547 3102 2547
Size: 555 6673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 4334 4398 4535 5888 1239
Size: 816 2279 64 137 1353 2579
Fragments arranged by size:
2579 2279 1353 816 137 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3566 5353 6218 6516 3566
Size: 1787 865 298 4278
Fragments arranged by size:
4278 1787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3073 3308 5891 882
Size: 617 1574 235 2583 2219
Fragments arranged by size:
2583 2219 1574 617 235
BsaAI yAC'GTr
Cuts at: 493 1204 5019 493
Size: 711 3815 2702
Fragments arranged by size:
3815 2702 711
BsaBI GATnn'nnATC
Cuts at: 7222 7222
Size: 7228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5035 5417 6052 275
Size: 53 83 186 936 3502 382 635 1451
Fragments arranged by size:
3502 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5661 6059 6333 6480 6891 5661
Size: 398 274 147 411 5998
Fragments arranged by size:
5998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7003
Fragments arranged by size:
7003 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3075 3404 1186
Size: 1889 329 5010
Fragments arranged by size:
5010 1889 329
BseYI C'CCAG_C
Cuts at: 6431 6927 6431
Size: 496 6732
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3625 1163
Size: 573 1889 4766
Fragments arranged by size:
4766 1889 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4678 5439 6467 1540
Size: 275 186 2677 761 1028 2301
Fragments arranged by size:
2677 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 5289 5374 6445 2079
Size: 3210 85 1071 2862
Fragments arranged by size:
3210 2862 1071 85
BsmI GAATG_Cn'
Cuts at: 4739 4832 4739
Size: 93 7135
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 4058 4384 4547 5289 5374 6445
Size: 399 289 1979 326 163 742 85 1071
Cuts at: 6445 1391
Size: 2174
Fragments arranged by size:
2174 1979 1071 742 399 326 289 163
85
BspEI T'CCGG_A
Cuts at: 6891 6891
Size: 7228
BspHI T'CATG_A
Cuts at: 5117 6847 5117
Size: 1730 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4583 844
Size: 3739 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5115 1394
Size: 429 3292 3507
Fragments arranged by size:
3507 3292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2594 3599 4338 5723 5897 65
Size: 1183 1346 1005 739 1385 174 1396
Fragments arranged by size:
1396 1385 1346 1183 1005 739 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5872 6059 1700
Size: 110 4062 187 2869
Fragments arranged by size:
4062 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4269 96
Size: 4173 3055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7228
BssSI C'ACGA_G
Cuts at: 5288 6300 5288
Size: 1012 6216
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7228
BstBI TT'CG_AA
Cuts at: 6032 6032
Size: 7228
BstEII G'GTnAC_C
Cuts at: 1184 3263 3754 1184
Size: 2079 491 4658
Fragments arranged by size:
4658 2079 491
BstYI r'GATC_y
Cuts at: 1972 2569 2638 2836 3073 4558 4925 5310 5327
Size: 597 69 198 237 1485 367 385 17
Cuts at: 5327 6779 6865 6877 7223 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1485 1452 597 385 367 346 237
198 86 69 17 12
BstZ17I GTA'TAC
Cuts at: 2774 2774
Size: 7228
Bsu36I CC'TnA_GG
Cuts at: 4328 4328
Size: 7228
BtgI C'CryG_G
Cuts at: 513 1065 2401 4059 513
Size: 552 1336 1658 3682
Fragments arranged by size:
3682 1658 1336 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6489
Fragments arranged by size:
6489 683 56
BtsI GCAGTG_nn'
Cuts at: 4744 5538 5558 4744
Size: 794 20 6414
Fragments arranged by size:
6414 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7193
DraI TTT'AAA
Cuts at: 2505 2764 3402 4532 2505
Size: 259 638 1130 5201
Fragments arranged by size:
5201 1130 638 259
DraIII CAC_nnn'GTG
Cuts at: 4051 4051
Size: 7228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6235 809
Size: 507 4919 1802
Fragments arranged by size:
4919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2626 3988 4675
Size: 54 1425 50 275 18 796 1362 687
Cuts at: 4675 5564 7193 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1362 889 796 687 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4675 1537
Size: 275 2863 4090
Fragments arranged by size:
4090 2863 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4101 4110 4383 5158 1353
Size: 2748 9 273 775 3423
Fragments arranged by size:
3423 2748 775 273 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5815 6189 6335 387
Size: 1064 138 4226 374 146 1280
Fragments arranged by size:
4226 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2627 2826 3956 4286 4334 4398
Size: 393 423 572 199 1130 330 48 64
Cuts at: 4398 4415 4535 5291 5888 6675 6943 1239
Size: 17 120 756 597 787 268 1524
Fragments arranged by size:
1524 1130 787 756 597 572 423 393
330 268 199 120 64 48 17
EcoO109I rG'GnC_Cy
Cuts at: 2981 2981
Size: 7228
EcoRI G'AATT_C
Cuts at: 2420 4537 4915 2420
Size: 2117 378 4733
Fragments arranged by size:
4733 2117 378
FspI TGC'GCA
Cuts at: 5734 5734
Size: 7228
HaeII r_GCGC'y
Cuts at: 1678 6375 1678
Size: 4697 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4247 4279 5869 5901 5943
Size: 32 135 32 2675 32 1590 32 42
Cuts at: 5943 5975 1373
Size: 32 2626
Fragments arranged by size:
2675 2626 1590 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 4572 4818 669
Size: 988 281 957 1677 246 3079
Fragments arranged by size:
3079 1677 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 2212 3064 748
Size: 1464 852 4912
Fragments arranged by size:
4912 1464 852
HpaI GTT'AAC
Cuts at: 4818 4818
Size: 7228
KpnI G_GTAC'C
Cuts at: 4553 4553
Size: 7228
MfeI C'AATT_G
Cuts at: 2110 2671 4827 2110
Size: 561 2156 4511
Fragments arranged by size:
4511 2156 561
MluI A'CGCG_T
Cuts at: 7104 7104
Size: 7228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2230 2628 2682 3562 6342 6526 1305
Size: 925 398 54 880 2780 184 2007
Fragments arranged by size:
2780 2007 925 880 398 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3990 10
Size: 54 1425 343 2158 3248
Fragments arranged by size:
3248 2158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 5186 5545 5704 518
Size: 648 1081 2939 359 159 2042
Fragments arranged by size:
2939 2042 1081 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4644 5321 6469 6714 1606
Size: 3038 677 1148 245 2120
Fragments arranged by size:
3038 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2401 4059 513
Size: 552 1336 1658 3682
Fragments arranged by size:
3682 1658 1336 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7228
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7228
NotI GC'GGCC_GC
Cuts at: 4675 4675
Size: 7228
NspI r_CATG'y
Cuts at: 1132 1229 2713 2893 3295 3985 4490 4586 6131
Size: 97 1484 180 402 690 505 96 1545
Cuts at: 6131 1132
Size: 2229
Fragments arranged by size:
2229 1545 1484 690 505 402 180 97
96
PasI CC'CwG_GG
Cuts at: 1348 3558 1348
Size: 2210 5018
PciI A'CATG_T
Cuts at: 6127 6127
Size: 7228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 4088 1108
Size: 1299 1681 4248
Fragments arranged by size:
4248 1681 1299
PfoI T'CCnGG_A
Cuts at: 2977 4375 4512 2977
Size: 1398 137 5693
Fragments arranged by size:
5693 1398 137
PmeI GTTT'AAAC
Cuts at: 4532 4532
Size: 7228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5297 5329 6810 6842 5297
Size: 32 1481 32 5683
Fragments arranged by size:
5683 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2981 2981
Size: 7228
PshAI GACnn'nnGTC
Cuts at: 1613 3957 1613
Size: 2344 4884
PsiI TTA'TAA
Cuts at: 4798 4798
Size: 7228
PstI C_TGCA'G
Cuts at: 830 3591 3883 4580 830
Size: 2761 292 697 3478
Fragments arranged by size:
3478 2761 697 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7228
SalI G'TCGA_C
Cuts at: 1936 4570 1936
Size: 2634 4594
SapI GCTCTTCn'nnn_
Cuts at: 4383 4383
Size: 7228
SbfI CC_TGCA'GG
Cuts at: 4580 4580
Size: 7228
ScaI AGT'ACT
Cuts at: 5476 7188 5476
Size: 1712 5516
SexAI A'CCwGG_T
Cuts at: 2935 3576 2935
Size: 641 6587
SfcI C'TryA_G
Cuts at: 826 1045 3587 3879 4072 4576 5711 6392 6583
Size: 219 2542 292 193 504 1135 681 191
Cuts at: 6583 826
Size: 1471
Fragments arranged by size:
2542 1471 1135 681 504 292 219 193
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7228
SmaI CCC'GGG
Cuts at: 1872 3652 4555 1872
Size: 1780 903 4545
Fragments arranged by size:
4545 1780 903
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3545 5332 6233 6495 820
Size: 197 926 1602 1787 901 262 1553
Fragments arranged by size:
1787 1602 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5019 493
Size: 4526 2702
SpeI A'CTAG_T
Cuts at: 152 3740 152
Size: 3588 3640
SphI G_CATG'C
Cuts at: 1132 4586 1132
Size: 3454 3774
SspI AAT'ATT
Cuts at: 5 52 2302 2634 3124 3161 3508 3837 5152
Size: 47 2250 332 490 37 347 329 1315
Cuts at: 5152 5
Size: 2081
Fragments arranged by size:
2250 2081 1315 490 347 332 329 47
37
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7228
StyI C'CwwG_G
Cuts at: 513 1065 2287 2335 2401 3298 4059 4427 4667
Size: 552 1222 48 66 897 761 368 240
Cuts at: 4667 513
Size: 3074
Fragments arranged by size:
3074 1222 897 761 552 368 240 66
48
SwaI ATTT'AAAT
Cuts at: 3402 3402
Size: 7228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5255 5272 5425 5610 6055 325
Size: 1255 3675 17 153 185 445 1498
Fragments arranged by size:
3675 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3975 4269 4416
Size: 274 80 33 51 1443 1998 294 147
Cuts at: 4416 5474 7186 96
Size: 1058 1712 138
Fragments arranged by size:
1998 1712 1443 1058 294 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 2959 2991 7188 7220 2187
Size: 32 740 32 4197 32 2195
Fragments arranged by size:
4197 2195 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2905 1316
Size: 1589 5639
XbaI T'CTAG_A
Cuts at: 2566 4564 2566
Size: 1998 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7228
XmaI C'CCGG_G
Cuts at: 1870 3650 4553 1870
Size: 1780 903 4545
Fragments arranged by size:
4545 1780 903
XmnI GAAnn'nnTTC
Cuts at: 3177 5357 3177
Size: 2180 5048
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5036 276
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI XcmI