Restriction map for
pFN21AB8625
(Circular) (Six-base) MAPSORT of: pFN21AB8625.seq Check: 2738 from: 1 to: 5698
pFN21AB8625 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 2997 3825 4198 1563
Size: 1434 828 373 3063
Fragments arranged by size:
3063 1434 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 2501 2898 2963 3761 5145 5413 1632
Size: 454 415 397 65 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 454 415 397 268 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2015 2047 2015
Size: 32 5666
AlwNI CAG_nnn'CTG
Cuts at: 2536 3441 5013 2536
Size: 905 1572 3221
Fragments arranged by size:
3221 1572 905
ApaI G_GGCC'C
Cuts at: 2310 2310
Size: 5698
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2821 2990 3007 3233 3385 5494 1493
Size: 1328 169 17 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 169 152 17
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
AvrII C'CTAG_G
Cuts at: 2491 2491
Size: 5698
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2619 3019 4474 618
Size: 325 217 627 832 400 1455 1842
Fragments arranged by size:
1842 1455 832 627 400 325 217
BanII G_rGCy'C
Cuts at: 1391 2230 2310 2637 2670 2699 3017 1391
Size: 839 80 327 33 29 318 4072
Fragments arranged by size:
4072 839 327 318 80 33 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 928
Size: 971 314 4413
Fragments arranged by size:
4413 971 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2428 3590 4800 2428
Size: 1162 1210 3326
Fragments arranged by size:
3326 1210 1162
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 4309 136
Size: 107 122 71 631 605 604 2033 1525
Fragments arranged by size:
2033 1525 631 605 604 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2058 3115 2058
Size: 1057 4641
Bme1580I G_kGCm'C
Cuts at: 1790 2310 3759 4915 1790
Size: 520 1449 1156 2573
Fragments arranged by size:
2573 1449 1156 520
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2759 4358 1239
Size: 1520 1599 2579
Fragments arranged by size:
2579 1599 1520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2621 3823 4688 4986 2384
Size: 237 1202 865 298 3096
Fragments arranged by size:
3096 1202 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 4361 882
Size: 617 792 2070 2219
Fragments arranged by size:
2219 2070 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 2961 5692 2961
Size: 2731 2967
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2965 4131 4529 4803 4950 5361 2965
Size: 1166 398 274 147 411 3302
Fragments arranged by size:
3302 1166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 2684 2714 1178
Size: 30 165 30 612 30 639 30 4162
Fragments arranged by size:
4162 639 612 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2039 2154 2465 2705 1186
Size: 850 3 115 311 240 4179
Fragments arranged by size:
4179 850 311 240 115 3
BseYI C'CCAG_C
Cuts at: 2394 4901 5397 2394
Size: 2507 496 2695
Fragments arranged by size:
2695 2507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 1163
Size: 573 363 4762
Fragments arranged by size:
4762 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 2699 3759 3844 4915 2670
Size: 29 1060 85 1071 3453
Fragments arranged by size:
3453 1071 1060 85 29
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2230 2310 2637 2670 2699 3017 3759
Size: 399 440 80 327 33 29 318 742
Cuts at: 3759 3844 4915 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 327 318 85
80 33 29
BspEI T'CCGG_A
Cuts at: 2965 5361 2965
Size: 2396 3302
BspHI T'CATG_A
Cuts at: 2386 3587 5317 2386
Size: 1201 1730 2767
Fragments arranged by size:
2767 1730 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3053 844
Size: 1673 536 3489
Fragments arranged by size:
3489 1673 536
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2245 2263 3585 1394
Size: 429 206 216 18 1322 3507
Fragments arranged by size:
3507 1322 429 216 206 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 2066 2698 3758 4770 2066
Size: 632 1060 1012 2994
Fragments arranged by size:
2994 1060 1012 632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2302 2302
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2898 513
Size: 552 939 48 846 3313
Fragments arranged by size:
3313 939 846 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2555 4705 809
Size: 507 1239 2150 1802
Fragments arranged by size:
2150 1802 1239 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2268 2277
Size: 54 1425 50 275 18 219 219 9
Cuts at: 2277 3145 4034 5663 8
Size: 868 889 1629 43
Fragments arranged by size:
1629 1425 889 868 275 219 219 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2328 3628 1353
Size: 863 112 1300 3423
Fragments arranged by size:
3423 1300 863 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2025 2273 4285 4659 4805 387
Size: 1064 138 436 248 2012 374 146 1280
Fragments arranged by size:
2012 1280 1064 436 374 248 146 138
EcoICRI GAG'CTC
Cuts at: 2668 2697 2668
Size: 29 5669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2501 2759 2898 2963 3761 4358
Size: 393 454 415 258 139 65 798 597
Cuts at: 4358 5145 5413 1239
Size: 787 268 1524
Fragments arranged by size:
1524 798 787 597 454 415 393 268
258 139 65
EcoO109I rG'GnC_Cy
Cuts at: 2306 2307 2306
Size: 1 5697
EcoRI G'AATT_C
Cuts at: 2821 3007 3385 2821
Size: 186 378 5134
Fragments arranged by size:
5134 378 186
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2719 2751 4339 4371 4413
Size: 32 135 32 1147 32 1588 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 1588 1147 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4284
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 4812 4996 1305
Size: 1215 2292 184 2007
Fragments arranged by size:
2292 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 2279 10
Size: 54 1425 343 219 228 3429
Fragments arranged by size:
3429 1425 343 228 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3656 4015 4174 518
Size: 648 1434 1056 359 159 2042
Fragments arranged by size:
2042 1434 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2523 2614 3114 3791 4939 5184 1606
Size: 591 326 91 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 500 326 245 91
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 2898 513
Size: 552 939 48 846 3313
Fragments arranged by size:
3313 939 846 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2044 3056 4601 1132
Size: 97 815 1012 1545 2229
Fragments arranged by size:
2229 1545 1012 815 97
PasI CC'CwG_GG
Cuts at: 1348 2423 1348
Size: 1075 4623
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2015 2047 3767 3799 5280 5312 2015
Size: 32 1720 32 1481 32 2401
Fragments arranged by size:
2401 1720 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2893 1613
Size: 1280 4418
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2306 2306
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2352 3050 830
Size: 1522 698 3478
Fragments arranged by size:
3478 1522 698
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2197 2197
Size: 5698
SacI G_AGCT'C
Cuts at: 2670 2699 2670
Size: 29 5669
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2348 3046 4181 4862 5053 826
Size: 219 1303 698 1135 681 191 1471
Fragments arranged by size:
1471 1303 1135 698 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2276 2276
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2636 3802 4703 4965 820
Size: 197 926 456 237 1166 901 262 1553
Fragments arranged by size:
1553 1166 926 901 456 262 237 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2280 2491 2898 2982 3137
Size: 552 939 48 228 211 407 84 155
Cuts at: 3137 513
Size: 3074
Fragments arranged by size:
3074 939 552 407 228 211 155 84
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 5658 5690 2684
Size: 32 2942 32 2692
Fragments arranged by size:
2942 2692 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2536 1316
Size: 1220 4478
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 2096 2948 3827 2096
Size: 852 879 3967
Fragments arranged by size:
3967 879 852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfoI SrfI SwaI XcmI