Restriction map for
pFN21AB8624
(Circular) (Six-base) MAPSORT of: pFN21AB8624.seq Check: 7712 from: 1 to: 5755
pFN21AB8624 5755 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2269 2444 2582 2644 2269
Size: 175 138 62 5380
Fragments arranged by size:
5380 175 138 62
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2525 3565 278
Size: 53 83 186 936 989 1040 2468
Fragments arranged by size:
2468 1040 989 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 3098 1937
Size: 253 908 4594
Fragments arranged by size:
4594 908 253
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 2314 3818 5202 5470 1632
Size: 609 73 1504 1384 268 1917
Fragments arranged by size:
1917 1504 1384 609 268 73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 3052 4654 5631 1203
Size: 1849 1602 977 1327
Fragments arranged by size:
1849 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 2173 2216 2827 3498 5070 2173
Size: 43 611 671 1572 2858
Fragments arranged by size:
2858 1572 671 611 43
ApaI G_GGCC'C
Cuts at: 2816 2933 2816
Size: 117 5638
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 2910 3085 3452 2910
Size: 175 367 5213
Fragments arranged by size:
5213 367 175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2033 2546 2570 2840
Size: 325 217 627 233 13 513 24 270
Cuts at: 2840 3076 4531 618
Size: 236 1455 1842
Fragments arranged by size:
1842 1455 627 513 325 270 236 233
217 24 13
BanII G_rGCy'C
Cuts at: 1391 2816 2933 3074 1391
Size: 1425 117 141 4072
Fragments arranged by size:
4072 1425 141 117
BbeI G_GCGC'C
Cuts at: 2024 2037 2024
Size: 13 5742
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2476 2525 928
Size: 971 577 49 4158
Fragments arranged by size:
4158 971 577 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2560 2594 3946 3980 1903
Size: 34 623 34 1352 34 3678
Fragments arranged by size:
3678 1352 623 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 4366 136
Size: 107 122 71 631 605 652 2042 1525
Fragments arranged by size:
2042 1525 652 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2095 2270 3172 2095
Size: 175 902 4678
Fragments arranged by size:
4678 902 175
Bme1580I G_kGCm'C
Cuts at: 1790 2549 2561 2816 2933 3816 4972 1790
Size: 759 12 255 117 883 1156 2573
Fragments arranged by size:
2573 1156 883 759 255 117 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2426 2945 4415 1239
Size: 1066 121 519 1470 2579
Fragments arranged by size:
2579 1470 1066 519 121
Bpu10I CC'TnA_GC
Cuts at: 2385 2385
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 2420 5749 2420
Size: 3329 2426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2034 2522 3562
Size: 53 83 186 936 488 13 488 1040
Cuts at: 3562 3944 4579 275
Size: 382 635 1451
Fragments arranged by size:
1451 1040 936 635 488 488 382 186
83 53 13
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2822 1186
Size: 1054 582 4119
Fragments arranged by size:
4119 1054 582
BseYI C'CCAG_C
Cuts at: 2303 2590 2668 4958 5454 2303
Size: 287 78 2290 496 2604
Fragments arranged by size:
2604 2290 496 287 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2549 2561 2816 2933 3074 3816 3901
Size: 399 759 12 255 117 141 742 85
Cuts at: 3901 4972 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 759 742 399 255 141 117
85 12
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2269 2444 2582 2644 3110 844
Size: 1425 175 138 62 466 3489
Fragments arranged by size:
3489 1425 466 175 138 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 4250 4424 65
Size: 1183 1441 1561 174 1396
Fragments arranged by size:
1561 1441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2274 4399 4586 1700
Size: 110 213 251 2125 187 2869
Fragments arranged by size:
2869 2125 251 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2207 2216 794
Size: 1413 9 4333
Fragments arranged by size:
4333 1413 9
BstBI TT'CG_AA
Cuts at: 2070 4559 2070
Size: 2489 3266
BstEII G'GTnAC_C
Cuts at: 1184 3037 1184
Size: 1853 3902
BstYI r'GATC_y
Cuts at: 1972 2910 3085 3452 3837 3854 5306 5392 5404
Size: 938 175 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 938 385 367 346 175 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2131 2131
Size: 5755
BtgI C'CryG_G
Cuts at: 513 1065 2004 2509 2808 513
Size: 552 939 505 299 3460
Fragments arranged by size:
3460 939 552 505 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2663 2774 2796 3271 4065 4085 2663
Size: 111 22 475 794 20 4333
Fragments arranged by size:
4333 794 475 111 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2899 2899
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3046 4762 809
Size: 507 1730 1716 1802
Fragments arranged by size:
1802 1730 1716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 2741 2750 2870 3685 1353
Size: 864 524 9 120 815 3423
Fragments arranged by size:
3423 864 815 524 120 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2103 4342 4716 4862 387
Size: 1064 138 514 2239 374 146 1280
Fragments arranged by size:
2239 1280 1064 514 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2305 2314 2426 2945 3818 4415
Size: 393 609 64 9 112 519 873 597
Cuts at: 4415 5202 5470 1239
Size: 787 268 1524
Fragments arranged by size:
1524 873 787 609 597 519 393 268
112 64 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2177
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2325 2813 2929 2930 2956 2325
Size: 488 116 1 26 5124
Fragments arranged by size:
5124 488 116 26 1
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 2780 4261 2780
Size: 1481 4274
HaeII r_GCGC'y
Cuts at: 1678 2024 2037 2173 4902 1678
Size: 346 13 136 2729 2531
Fragments arranged by size:
2729 2531 346 136 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 4396 4428 4470
Size: 32 135 32 542 32 2250 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 2250 542 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3099 3345 669
Size: 988 281 496 665 246 3079
Fragments arranged by size:
3079 988 665 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2020 2033 2020
Size: 13 5742
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3713 4072 4231 518
Size: 648 1386 1161 359 159 2042
Fragments arranged by size:
2042 1386 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2099 2204 2213 2269 2824 3171 3848
Size: 412 81 105 9 56 555 347 677
Cuts at: 3848 4996 5241 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 412 347 245 105
81 56 9
NaeI GCC'GGC
Cuts at: 2025 2276 2025
Size: 251 5504
NarI GG'CG_CC
Cuts at: 2021 2034 2021
Size: 13 5742
NcoI C'CATG_G
Cuts at: 513 1065 2004 2509 513
Size: 552 939 505 3759
Fragments arranged by size:
3759 939 552 505
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2023 2274 2023
Size: 251 5504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2863 3113 4658 1132
Size: 97 1634 250 1545 2229
Fragments arranged by size:
2229 1634 1545 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2899 1108
Size: 1791 3964
PfoI T'CCnGG_A
Cuts at: 2403 2538 2913 2403
Size: 135 375 5245
Fragments arranged by size:
5245 375 135
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2956 2956
Size: 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2812 2929 2812
Size: 117 5638
PstI C_TGCA'G
Cuts at: 830 2655 3048 3107 830
Size: 1825 393 59 3478
Fragments arranged by size:
3478 1825 393 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2099 2204 2213 2269 2099
Size: 105 9 56 5585
Fragments arranged by size:
5585 105 56 9
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SanDI GG'GwC_CC
Cuts at: 2956 2956
Size: 5755
SapI GCTCTTCn'nnn_
Cuts at: 2870 2870
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2013 2651 3044 3103 4238 4919 5110
Size: 219 968 638 393 59 1135 681 191
Cuts at: 5110 826
Size: 1471
Fragments arranged by size:
1471 1135 968 681 638 393 219 191
59
SfoI GGC'GCC
Cuts at: 2022 2035 2022
Size: 13 5742
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 2023 1700
Size: 323 5432
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2509 3194 513
Size: 552 939 505 685 3074
Fragments arranged by size:
3074 939 685 552 505
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2144 2750 3782 3799 3952 4137 4582
Size: 1255 564 606 1032 17 153 185 445
Cuts at: 4582 325
Size: 1498
Fragments arranged by size:
1498 1255 1032 606 564 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2779 2779
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2523 3563 276
Size: 53 83 186 936 989 1040 2468
Fragments arranged by size:
2468 1040 989 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI SacI SacII
SexAI SfiI SrfI SwaI