Restriction map for
pFN21AB8622
(Circular) (Six-base) MAPSORT of: pFN21AB8622.seq Check: 4503 from: 1 to: 7222
pFN21AB8622 7222 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3018 3018
Size: 7222
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2427 3390 5032 278
Size: 53 83 186 936 891 963 1642 2468
Fragments arranged by size:
2468 1642 963 936 891 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 4565 1937
Size: 490 2138 4594
Fragments arranged by size:
4594 2138 490
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 4288 5349 5722 1563
Size: 2725 1061 373 3063
Fragments arranged by size:
3063 2725 1061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2592 2893 2957 4250 5285 6669 6937 1632
Size: 960 301 64 1293 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1293 1035 960 301 268 64
AfeI AGC'GCT
Cuts at: 4091 4091
Size: 7222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7025
AflIII A'CryG_T
Cuts at: 1203 2766 3561 3630 6121 7098 1203
Size: 1563 795 69 2491 977 1327
Fragments arranged by size:
2491 1563 1327 977 795 69
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 3847 5951 3847
Size: 2104 5118
AleI CACnn'nnGTG
Cuts at: 1166 4349 1166
Size: 3183 4039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 6337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3213 3245 3213
Size: 32 7190
AlwNI CAG_nnn'CTG
Cuts at: 2113 2143 2212 2890 4965 6537 2113
Size: 30 69 678 2075 1572 2798
Fragments arranged by size:
2798 2075 1572 678 69 30
ApaLI G'TGCA_C
Cuts at: 2929 5279 6435 2929
Size: 2350 1156 3716
Fragments arranged by size:
3716 2350 1156
ApoI r'AATT_y
Cuts at: 1493 2258 2954 3152 3172 3194 4181 4531 4757
Size: 765 696 198 20 22 987 350 226
Cuts at: 4757 4909 7018 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 987 765 696 350 226 198
152 22 20
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 3963 4281 4374 4547 1870
Size: 73 1842 178 318 93 173 4545
Fragments arranged by size:
4545 1842 318 178 173 93 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3619 3652 4250 4283 3619
Size: 33 598 33 6558
Fragments arranged by size:
6558 598 33 33
BamHI G'GATC_C
Cuts at: 4158 4552 4919 4158
Size: 394 367 6461
Fragments arranged by size:
6461 394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3611 4543 5998 618
Size: 325 217 627 1824 932 1455 1842
Fragments arranged by size:
1842 1824 1455 932 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2669 2690 3327 4541 1391
Size: 1278 21 637 1214 4072
Fragments arranged by size:
4072 1278 1214 637 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 2701 2797 3208 928
Size: 971 187 615 96 411 4942
Fragments arranged by size:
4942 971 615 411 187 96
BbvCI CC'TCA_GC
Cuts at: 3833 3833
Size: 7222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3962 3996 5413 5447 1903
Size: 34 2025 34 1417 34 3678
Fragments arranged by size:
3678 2025 1417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 5114 6324 3620
Size: 1494 1210 4518
Fragments arranged by size:
4518 1494 1210
BclI T'GATC_A
Cuts at: 1548 2730 4062 1548
Size: 1182 1332 4708
Fragments arranged by size:
4708 1332 1182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3055 4301 4434
Size: 107 122 71 631 605 1383 1246 133
Cuts at: 4434 5833 136
Size: 1399 1525
Fragments arranged by size:
1525 1399 1383 1246 631 605 133 122
107 71
BglII A'GATC_T
Cuts at: 3709 7217 3709
Size: 3508 3714
BlpI GC'TnA_GC
Cuts at: 2684 4086 4639 2684
Size: 1402 553 5267
Fragments arranged by size:
5267 1402 553
Bme1580I G_kGCm'C
Cuts at: 1790 2933 3614 3658 5283 6439 1790
Size: 1143 681 44 1625 1156 2573
Fragments arranged by size:
2573 1625 1156 1143 681 44
BmgBI CAC'GTC
Cuts at: 1318 2767 3820 4411 1318
Size: 1449 1053 591 4129
Fragments arranged by size:
4129 1449 1053 591
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 4313 4953
Size: 416 53 83 399 24 783 2113 640
Cuts at: 4953 5911 442
Size: 958 1753
Fragments arranged by size:
2113 1753 958 783 640 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 2621 2660 2741 3209 4217 4282 4291
Size: 1220 162 39 81 468 1008 65 9
Cuts at: 4291 5882 1239
Size: 1591 2579
Fragments arranged by size:
2579 1591 1220 1008 468 162 81 65
39 9
Bpu10I CC'TnA_GC
Cuts at: 3833 3833
Size: 7222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 3157 3311 3845 4019 4247 5347 6212 6510
Size: 998 154 534 174 228 1100 865 298
Cuts at: 6510 2159
Size: 2871
Fragments arranged by size:
2871 1100 998 865 534 298 228 174
154
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 2606 3125 4434 4457 5885 882
Size: 617 897 210 519 1309 23 1428 2219
Fragments arranged by size:
2219 1428 1309 897 617 519 210 23
BsaAI yAC'GTr
Cuts at: 493 1204 4501 5013 493
Size: 711 3297 512 2702
Fragments arranged by size:
3297 2702 711 512
BsaBI GATnn'nnATC
Cuts at: 3104 4163 7216 3104
Size: 1059 3053 3110
Fragments arranged by size:
3110 3053 1059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2424 3387 3648 3717
Size: 53 83 186 936 891 963 261 69
Cuts at: 3717 5029 5411 6046 275
Size: 1312 382 635 1451
Fragments arranged by size:
1451 1312 963 936 891 635 382 261
186 83 69 53
BsaWI w'CCGG_w
Cuts at: 3231 3722 5655 6053 6327 6474 6885 3231
Size: 491 1933 398 274 147 411 3568
Fragments arranged by size:
3568 1933 491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 1178
Size: 30 165 30 735 30 6232
Fragments arranged by size:
6232 735 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2456 2939 4523 1186
Size: 1228 42 483 1584 3885
Fragments arranged by size:
3885 1584 1228 483 42
BseYI C'CCAG_C
Cuts at: 3775 3902 6425 6921 3775
Size: 127 2523 496 4076
Fragments arranged by size:
4076 2523 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2339 2564 1163
Size: 573 393 210 225 5821
Fragments arranged by size:
5821 573 393 225 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 2088 2195 3956 4672 5433
Size: 275 186 28 59 107 1761 716 761
Cuts at: 5433 6461 1540
Size: 1028 2301
Fragments arranged by size:
2301 1761 1028 761 716 275 186 107
59 28
BsiHKAI G_wGCw'C
Cuts at: 2865 2933 3000 3327 5283 5368 6439 2865
Size: 68 67 327 1956 85 1071 3648
Fragments arranged by size:
3648 1956 1071 327 85 68 67
BsiWI C'GTAC_G
Cuts at: 4497 4497
Size: 7222
BsmI GAATG_Cn'
Cuts at: 4733 4826 4733
Size: 93 7129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2669 2690 2865 2933 3000 3327 3614
Size: 399 879 21 175 68 67 327 287
Cuts at: 3614 3658 4541 5283 5368 6439 1391
Size: 44 883 742 85 1071 2174
Fragments arranged by size:
2174 1071 883 879 742 399 327 287
175 85 68 67 44 21
BspEI T'CCGG_A
Cuts at: 3722 6885 3722
Size: 3163 4059
BspHI T'CATG_A
Cuts at: 5111 6841 5111
Size: 1730 5492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2979 3018 3989 4038 4577 844
Size: 2135 39 971 49 539 3489
Fragments arranged by size:
3489 2135 971 539 49 39
BsrBI CCG'CTC
Cuts at: 1394 1823 3728 4072 5109 1394
Size: 429 1905 344 1037 3507
Fragments arranged by size:
3507 1905 1037 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 5717 5891 65
Size: 1183 4469 174 1396
Fragments arranged by size:
4469 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3951 4311 5866 6053 1700
Size: 110 2141 360 1555 187 2869
Fragments arranged by size:
2869 2141 1555 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 3102 3558 96
Size: 3006 456 3760
Fragments arranged by size:
3760 3006 456
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 5282 6294 5282
Size: 1012 6210
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2113 2971 2995 3591 794
Size: 1319 858 24 596 4425
Fragments arranged by size:
4425 1319 858 596 24
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7222
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 3748 2560
Size: 1188 6034
BstYI r'GATC_y
Cuts at: 1972 2604 3709 4158 4419 4552 4919 5304 5321
Size: 632 1105 449 261 133 367 385 17
Cuts at: 5321 6773 6859 6871 7217 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1105 632 449 385 367 346
261 133 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2753 3605 2753
Size: 852 6370
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2334 3766 3854 513
Size: 552 939 84 246 1432 88 3881
Fragments arranged by size:
3881 1432 939 552 246 88 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2347 3113 487
Size: 683 56 1121 766 4596
Fragments arranged by size:
4596 1121 766 683 56
BtsI GCAGTG_nn'
Cuts at: 2018 3978 4738 5532 5552 2018
Size: 1960 760 794 20 3688
Fragments arranged by size:
3688 1960 794 760 20
ClaI AT'CG_AT
Cuts at: 1554 2040 1554
Size: 486 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7187
DraI TTT'AAA
Cuts at: 4526 4526
Size: 7222
DraIII CAC_nnn'GTG
Cuts at: 2071 2071
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2423 3287 6229 809
Size: 507 1107 864 2942 1802
Fragments arranged by size:
2942 1802 1107 864 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2085 2192
Size: 54 1425 50 275 18 196 59 107
Cuts at: 2192 2448 2796 2892 3501 3750 3953 4353 4669
Size: 256 348 96 609 249 203 400 316
Cuts at: 4669 5558 7187 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 609 400 348 316 275
256 249 203 196 107 96 59 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2026 2085 2192 3953 4669 1537
Size: 275 214 59 107 1761 716 4090
Fragments arranged by size:
4090 1761 716 275 214 107 59
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 3467 4500 5152 1353
Size: 953 1161 1033 652 3423
Fragments arranged by size:
3423 1161 1033 953 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2708 4452 5809 6183 6329 387
Size: 1064 138 1119 1744 1357 374 146 1280
Fragments arranged by size:
1744 1357 1280 1119 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3325 3325
Size: 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 2592 2621 2660 2741 2893 2957
Size: 393 827 133 29 39 81 152 64
Cuts at: 2957 3209 4217 4250 4282 4291 5285 5882 6669
Size: 252 1008 33 32 9 994 597 787
Cuts at: 6669 6937 1239
Size: 268 1524
Fragments arranged by size:
1524 1008 994 827 787 597 393 268
252 152 133 81 64 39 33 32
29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2468 2732 2105
Size: 363 264 6595
Fragments arranged by size:
6595 363 264
EcoO109I rG'GnC_Cy
Cuts at: 2050 2125 2725 3737 4053 4302 2050
Size: 75 600 1012 316 249 4970
Fragments arranged by size:
4970 1012 600 316 249 75
EcoRI G'AATT_C
Cuts at: 4531 4909 4531
Size: 378 6844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3151 3457 4255 3151
Size: 306 798 6118
Fragments arranged by size:
6118 798 306
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 2114 4093 6369 1678
Size: 436 1979 2276 2531
Fragments arranged by size:
2531 2276 1979 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3833 3865 5863 5895 5937
Size: 32 135 32 2261 32 1998 32 42
Cuts at: 5937 5969 1373
Size: 32 2626
Fragments arranged by size:
2626 2261 1998 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 4566 4812 669
Size: 988 281 1306 1322 246 3079
Fragments arranged by size:
3079 1322 1306 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7222
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 4821 4821
Size: 7222
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6336 6520 1305
Size: 5031 184 2007
Fragments arranged by size:
5031 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2798 2894 3503 3752 4355
Size: 54 1425 343 966 96 609 249 603
Cuts at: 4355 10
Size: 2877
Fragments arranged by size:
2877 1425 966 609 603 343 249 96
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2558 2765 4136 4349 5180 5539
Size: 648 1368 24 207 1371 213 831 359
Cuts at: 5539 5698 518
Size: 159 2042
Fragments arranged by size:
2042 1371 1368 831 648 359 213 207
159 24
MspA1I CmG'CkG
Cuts at: 1606 2031 2140 2336 2705 4050 4249 4349 4638
Size: 425 109 196 369 1345 199 100 289
Cuts at: 4638 5315 6463 6708 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1345 1148 677 425 369 289 245
199 196 109 100
NaeI GCC'GGC
Cuts at: 3953 3953
Size: 7222
NcoI C'CATG_G
Cuts at: 513 1065 2004 3766 513
Size: 552 939 1762 3969
Fragments arranged by size:
3969 1762 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7222
NgoMIV G'CCGG_C
Cuts at: 3951 3951
Size: 7222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 2026 4669 2026
Size: 2643 4579
NspI r_CATG'y
Cuts at: 1132 1229 3634 4580 6125 1132
Size: 97 2405 946 1545 2229
Fragments arranged by size:
2405 2229 1545 946 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7222
PciI A'CATG_T
Cuts at: 3630 6121 3630
Size: 2491 4731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 2890 1108
Size: 963 819 5440
Fragments arranged by size:
5440 963 819
PfoI T'CCnGG_A
Cuts at: 2607 2943 4194 2607
Size: 336 1251 5635
Fragments arranged by size:
5635 1251 336
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 5291 5323 6804 6836 3332
Size: 32 1927 32 1481 32 3718
Fragments arranged by size:
3718 1927 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2125 2725 3737 4053 2050
Size: 75 600 1012 316 5219
Fragments arranged by size:
5219 1012 600 316 75
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 4792 4792
Size: 7222
PstI C_TGCA'G
Cuts at: 830 2852 3709 4574 830
Size: 2022 857 865 3478
Fragments arranged by size:
3478 2022 865 857
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
PvuII CAG'CTG
Cuts at: 2140 2705 4249 4349 2140
Size: 565 1544 100 5013
Fragments arranged by size:
5013 1544 565 100
SacI G_AGCT'C
Cuts at: 3327 3327
Size: 7222
SacII CC_GC'GG
Cuts at: 2337 2337
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SbfI CC_TGCA'GG
Cuts at: 4574 4574
Size: 7222
ScaI AGT'ACT
Cuts at: 2627 2846 4211 5470 7182 2627
Size: 219 1365 1259 1712 2667
Fragments arranged by size:
2667 1712 1365 1259 219
SexAI A'CCwGG_T
Cuts at: 3733 3733
Size: 7222
SfcI C'TryA_G
Cuts at: 826 1045 2848 3165 3313 3705 4423 4570 5705
Size: 219 1803 317 148 392 718 147 1135
Cuts at: 5705 6386 6577 826
Size: 681 191 1471
Fragments arranged by size:
1803 1471 1135 718 681 392 317 219
191 148 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 4301 4301
Size: 7222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 3787 3965 4283 4549 1872
Size: 1915 178 318 266 4545
Fragments arranged by size:
4545 1915 318 266 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 3136 3326 3824 4034 4262
Size: 197 926 231 962 190 498 210 228
Cuts at: 4262 5326 6227 6489 820
Size: 1064 901 262 1553
Fragments arranged by size:
1553 1064 962 926 901 498 262 231
228 210 197 190
SnaBI TAC'GTA
Cuts at: 493 4501 5013 493
Size: 4008 512 2702
Fragments arranged by size:
4008 2702 512
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4580 1132
Size: 3448 3774
SspI AAT'ATT
Cuts at: 5 52 5146 5
Size: 47 5094 2081
Fragments arranged by size:
5094 2081 47
StuI AGG'CCT
Cuts at: 1517 2386 1517
Size: 869 6353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2799 3766 4661 513
Size: 552 939 795 967 895 3074
Fragments arranged by size:
3074 967 939 895 795 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 3377 5249 5266 5419 5604 6049
Size: 1255 1050 747 1872 17 153 185 445
Cuts at: 6049 325
Size: 1498
Fragments arranged by size:
1872 1498 1255 1050 747 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 2844 3102
Size: 274 80 33 51 1443 648 219 258
Cuts at: 3102 3558 4209 5468 7180 96
Size: 456 651 1259 1712 138
Fragments arranged by size:
1712 1443 1259 651 648 456 274 258
219 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2925 2957 7182 7214 2138
Size: 32 755 32 4225 32 2146
Fragments arranged by size:
4225 2146 755 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 2486 3735 1316
Size: 807 363 1249 4803
Fragments arranged by size:
4803 1249 807 363
XbaI T'CTAG_A
Cuts at: 4558 4558
Size: 7222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2258 3091 2068
Size: 190 833 6199
Fragments arranged by size:
6199 833 190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 3785 3963 4281 4547 1870
Size: 1915 178 318 266 4545
Fragments arranged by size:
4545 1915 318 266 178
XmnI GAAnn'nnTTC
Cuts at: 2826 5351 2826
Size: 2525 4697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2425 3388 5030 276
Size: 53 83 186 936 891 963 1642 2468
Fragments arranged by size:
2468 1642 963 936 891 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BfrBI BplI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfoI
SrfI SwaI