Restriction map for
pFN21AB8621
(Circular) (Six-base) MAPSORT of: pFN21AB8621.seq Check: 8014 from: 1 to: 7315
pFN21AB8621 7315 bp
With 182 enzymes: SgfI *
March 28, 2012 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5125 278
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4658 1937
Size: 2721 4594
Acc65I G'GTAC_C
Cuts at: 2713 4636 2713
Size: 1923 5392
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 5378 6762 7030 1632
Size: 1205 2541 1384 268 1917
Fragments arranged by size:
2541 1917 1384 1205 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 3976 6214 7191 1203
Size: 2773 2238 977 1327
Fragments arranged by size:
2773 2238 1327 977
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 6044 6044
Size: 7315
AleI CACnn'nnGTG
Cuts at: 1166 2795 2881 3059 1166
Size: 1629 86 178 5422
Fragments arranged by size:
5422 1629 178 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7315
AlwNI CAG_nnn'CTG
Cuts at: 3967 5058 6630 3967
Size: 1091 1572 4652
Fragments arranged by size:
4652 1572 1091
ApaLI G'TGCA_C
Cuts at: 5372 6528 5372
Size: 1156 6159
ApoI r'AATT_y
Cuts at: 1493 2008 2227 2731 2752 3301 3319 3333 3438
Size: 515 219 504 21 549 18 14 105
Cuts at: 3438 3474 4184 4472 4624 4850 5002 7111 1493
Size: 36 710 288 152 226 152 2109 1697
Fragments arranged by size:
2109 1697 710 549 515 504 288 226
219 152 152 105 36 21 18 14
AseI AT'TA_AT
Cuts at: 160 1032 4539 5869 160
Size: 872 3507 1330 1606
Fragments arranged by size:
3507 1606 1330 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 4640 1870
Size: 73 2697 4545
Fragments arranged by size:
4545 2697 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4273 4306 4273
Size: 33 7282
BamHI G'GATC_C
Cuts at: 4645 5012 4645
Size: 367 6948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 2713 4636 6091 618
Size: 325 217 627 734 192 1923 1455 1842
Fragments arranged by size:
1923 1842 1455 734 627 325 217 192
BanII G_rGCy'C
Cuts at: 1391 4634 1391
Size: 3243 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 4069 4183 928
Size: 971 766 1404 114 4060
Fragments arranged by size:
4060 1404 971 766 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5506 5540 1903
Size: 34 3569 34 3678
Fragments arranged by size:
3678 3569 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2447 4301 5207 6417 2447
Size: 1854 906 1210 3345
Fragments arranged by size:
3345 1854 1210 906
BclI T'GATC_A
Cuts at: 1548 3567 1548
Size: 2019 5296
BfrBI ATG'CAT
Cuts at: 2954 2954
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5926 136
Size: 107 122 71 631 605 4254 1525
Fragments arranged by size:
4254 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 4732 4732
Size: 7315
Bme1580I G_kGCm'C
Cuts at: 1790 5376 6532 1790
Size: 3586 1156 2573
Fragments arranged by size:
3586 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5046 6004 442
Size: 416 53 83 399 24 3629 958 1753
Fragments arranged by size:
3629 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2486 5975 1239
Size: 982 265 3489 2579
Fragments arranged by size:
3489 2579 982 265
Bpu10I CC'TnA_GC
Cuts at: 3120 3120
Size: 7315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5440 6305 6603 5440
Size: 865 298 6152
Fragments arranged by size:
6152 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 3198 3951 5978 882
Size: 617 1293 406 753 2027 2219
Fragments arranged by size:
2219 2027 1293 753 617 406
BsaAI yAC'GTr
Cuts at: 493 1204 5106 493
Size: 711 3902 2702
Fragments arranged by size:
3902 2702 711
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5122 5504 6139 275
Size: 53 83 186 936 3589 382 635 1451
Fragments arranged by size:
3589 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5748 6146 6420 6567 6978 5748
Size: 398 274 147 411 6085
Fragments arranged by size:
6085 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 1178
Size: 30 165 30 1216 30 5844
Fragments arranged by size:
5844 1216 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3731 1186
Size: 2545 4770
BseYI C'CCAG_C
Cuts at: 3468 6518 7014 3468
Size: 3050 496 3769
Fragments arranged by size:
3769 3050 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2905 1163
Size: 573 1169 5573
Fragments arranged by size:
5573 1169 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 4765 5526 6554 1540
Size: 275 186 2398 366 761 1028 2301
Fragments arranged by size:
2398 2301 1028 761 366 275 186
BsiHKAI G_wGCw'C
Cuts at: 5376 5461 6532 5376
Size: 85 1071 6159
Fragments arranged by size:
6159 1071 85
BsmI GAATG_Cn'
Cuts at: 2153 4582 4826 4919 2153
Size: 2429 244 93 4549
Fragments arranged by size:
4549 2429 244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4634 5376 5461 6532 1391
Size: 399 2844 742 85 1071 2174
Fragments arranged by size:
2844 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 5204 6934 5204
Size: 1730 5585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 4670 844
Size: 1185 2641 3489
Fragments arranged by size:
3489 2641 1185
BsrBI CCG'CTC
Cuts at: 1394 1823 5202 1394
Size: 429 3379 3507
Fragments arranged by size:
3507 3379 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 3261 5810 5984 65
Size: 1183 789 1224 2549 174 1396
Fragments arranged by size:
2549 1396 1224 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5959 6146 1700
Size: 110 4149 187 2869
Fragments arranged by size:
4149 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3354 96
Size: 3258 4057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 5375 6387 5375
Size: 1012 6303
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7315
BstBI TT'CG_AA
Cuts at: 6119 6119
Size: 7315
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7315
BstXI CCAn_nnnn'nTGG
Cuts at: 4058 4058
Size: 7315
BstYI r'GATC_y
Cuts at: 1972 4645 5012 5397 5414 6866 6952 6964 7310
Size: 2673 367 385 17 1452 86 12 346
Cuts at: 7310 1972
Size: 1977
Fragments arranged by size:
2673 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3037 3037
Size: 7315
BtgI C'CryG_G
Cuts at: 513 1065 3792 4399 513
Size: 552 2727 607 3429
Fragments arranged by size:
3429 2727 607 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2668 487
Size: 683 56 1442 5134
Fragments arranged by size:
5134 1442 683 56
BtsI GCAGTG_nn'
Cuts at: 3811 4831 5625 5645 3811
Size: 1020 794 20 5481
Fragments arranged by size:
5481 1020 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7280
DraI TTT'AAA
Cuts at: 4619 4619
Size: 7315
DraIII CAC_nnn'GTG
Cuts at: 3688 3688
Size: 7315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4762 5651 7280
Size: 54 1425 50 275 18 2932 889 1629
Cuts at: 7280 8
Size: 43
Fragments arranged by size:
2932 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4762 1537
Size: 275 2950 4090
Fragments arranged by size:
4090 2950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5245 1353
Size: 3892 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5902 6276 6422 387
Size: 1064 138 4313 374 146 1280
Fragments arranged by size:
4313 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2486 2837 5378 5975 6762 7030
Size: 393 589 265 351 2541 597 787 268
Cuts at: 7030 1239
Size: 1524
Fragments arranged by size:
2541 1524 787 597 589 393 351 268
265
EcoRI G'AATT_C
Cuts at: 2008 3474 4184 4472 4624 5002 2008
Size: 1466 710 288 152 378 4321
Fragments arranged by size:
4321 1466 710 378 288 152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4403 4403
Size: 7315
FspI TGC'GCA
Cuts at: 5821 5821
Size: 7315
HaeII r_GCGC'y
Cuts at: 1678 6462 1678
Size: 4784 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2619 2651 2979 3011 3178
Size: 32 135 32 1047 32 328 32 167
Cuts at: 3178 3210 4571 4603 5956 5988 6030 6062 1373
Size: 32 1361 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1361 1353 1047 328 167 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4259 4659 4905 669
Size: 988 281 2321 400 246 3079
Fragments arranged by size:
3079 2321 988 400 281 246
HindIII A'AGCT_T
Cuts at: 748 2334 2469 748
Size: 1586 135 5594
Fragments arranged by size:
5594 1586 135
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KpnI G_GTAC'C
Cuts at: 2717 4640 2717
Size: 1923 5392
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3307 3427 4419 4474 4579 6429 6613 1305
Size: 2002 120 992 55 105 1850 184 2007
Fragments arranged by size:
2007 2002 1850 992 184 120 105 55
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5493
Fragments arranged by size:
5493 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2795 2881 3059 3626 5273 5632 5791
Size: 648 1629 86 178 567 1647 359 159
Cuts at: 5791 518
Size: 2042
Fragments arranged by size:
2042 1647 1629 648 567 359 178 159
86
MspA1I CmG'CkG
Cuts at: 1606 4731 5408 6556 6801 1606
Size: 3125 677 1148 245 2120
Fragments arranged by size:
3125 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3792 513
Size: 552 2727 4036
Fragments arranged by size:
4036 2727 552
NdeI CA'TA_TG
Cuts at: 387 3133 3240 387
Size: 2746 107 4462
Fragments arranged by size:
4462 2746 107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NsiI A_TGCA'T
Cuts at: 2956 2956
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 3980 4673 6218 1132
Size: 97 2751 693 1545 2229
Fragments arranged by size:
2751 2229 1545 693 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7315
PciI A'CATG_T
Cuts at: 3976 6214 3976
Size: 2238 5077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3688 1108
Size: 2580 4735
PfoI T'CCnGG_A
Cuts at: 4476 4476
Size: 7315
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 3032 4885 3032
Size: 1853 5462
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3930 3962 3930
Size: 32 7283
PstI C_TGCA'G
Cuts at: 830 2026 3596 4667 830
Size: 1196 1570 1071 3478
Fragments arranged by size:
3478 1570 1196 1071
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 2838 4487 5563 7275 2838
Size: 1649 1076 1712 2878
Fragments arranged by size:
2878 1712 1649 1076
SexAI A'CCwGG_T
Cuts at: 4359 4359
Size: 7315
SfcI C'TryA_G
Cuts at: 826 1045 2022 2067 2460 2550 2976 3367 3592
Size: 219 977 45 393 90 426 391 225
Cuts at: 3592 3947 4663 5798 6479 6670 826
Size: 355 716 1135 681 191 1471
Fragments arranged by size:
1471 1135 977 716 681 426 393 391
355 225 219 191 90 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 4642 1872
Size: 2770 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5419 6320 6582 820
Size: 197 926 3476 901 262 1553
Fragments arranged by size:
3476 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5106 493
Size: 4613 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4673 1132
Size: 3541 3774
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 3390 1517
Size: 1873 5442
StyI C'CwwG_G
Cuts at: 513 1065 3792 4754 513
Size: 552 2727 962 3074
Fragments arranged by size:
3074 2727 962 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5342 5359 5512 5697 6142 325
Size: 1255 3762 17 153 185 445 1498
Fragments arranged by size:
3762 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 2505 2836
Size: 274 80 33 51 1443 301 227 331
Cuts at: 2836 3354 4485 5561 7273 96
Size: 518 1131 1076 1712 138
Fragments arranged by size:
1712 1443 1131 1076 518 331 301 274
227 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7275 7307 7275
Size: 32 7283
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7315
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7315
XmaI C'CCGG_G
Cuts at: 1870 4640 1870
Size: 2770 4545
XmnI GAAnn'nnTTC
Cuts at: 2733 5444 2733
Size: 2711 4604
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5123 276
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI