Restriction map for
pFN21AB8617
(Circular) (Six-base) MAPSORT of: pFN21AB8617.seq Check: 560 from: 1 to: 6562
pFN21AB8617 6562 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2505 3905 1937
Size: 568 1400 4594
Fragments arranged by size:
4594 1400 568
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2286 2519 4625 6009 6277 1632
Size: 452 202 233 2106 1384 268 1917
Fragments arranged by size:
2106 1917 1384 452 268 233 202
AfeI AGC'GCT
Cuts at: 2300 2300
Size: 6562
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 2968 5461 6438 1203
Size: 1765 2493 977 1327
Fragments arranged by size:
2493 1765 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2565 2597 2565
Size: 32 6530
AlwNI CAG_nnn'CTG
Cuts at: 2569 2750 3199 4305 5877 2569
Size: 181 449 1106 1572 3254
Fragments arranged by size:
3254 1572 1106 449 181
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 2574 3309 3733 3871 4097 4249 6358 1493
Size: 1081 735 424 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 735 424 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 3887 1870
Size: 73 291 1653 4545
Fragments arranged by size:
4545 1653 291 73
BamHI G'GATC_C
Cuts at: 3707 3892 4259 3707
Size: 185 367 6010
Fragments arranged by size:
6010 367 185
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2677 3883 5338 618
Size: 325 217 627 890 1206 1455 1842
Fragments arranged by size:
1842 1455 1206 890 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2274 3026 3881 1391
Size: 883 752 855 4072
Fragments arranged by size:
4072 883 855 752
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 2547 3285 928
Size: 971 201 447 738 4205
Fragments arranged by size:
4205 971 738 447 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 4753 4787 1903
Size: 34 288 34 2494 34 3678
Fragments arranged by size:
3678 2494 288 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 3537 1548
Size: 1989 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 5173 136
Size: 107 122 71 631 605 530 2971 1525
Fragments arranged by size:
2971 1525 631 605 530 122 107 71
BglII A'GATC_T
Cuts at: 2018 3376 6557 2018
Size: 1358 3181 2023
Fragments arranged by size:
3181 2023 1358
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 4623 5779 1790
Size: 2833 1156 2573
Fragments arranged by size:
2833 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4293 5251 442
Size: 416 53 83 399 24 2876 958 1753
Fragments arranged by size:
2876 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3188 1056
Size: 2132 4430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2832 2832
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3246 3324 5222 1239
Size: 2007 78 1898 2579
Fragments arranged by size:
2579 2007 1898 78
Bpu10I CC'TnA_GC
Cuts at: 2755 2755
Size: 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4687 5552 5850 4687
Size: 865 298 5399
Fragments arranged by size:
5399 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5225 882
Size: 617 3726 2219
Fragments arranged by size:
3726 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4353 493
Size: 711 3149 2702
Fragments arranged by size:
3149 2702 711
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4369 4751 5386 275
Size: 53 83 186 936 2836 382 635 1451
Fragments arranged by size:
2836 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6337
Fragments arranged by size:
6337 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6562
BseYI C'CCAG_C
Cuts at: 2192 2301 2569 3283 5765 6261 2192
Size: 109 268 714 2482 496 2493
Fragments arranged by size:
2493 2482 714 496 268 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2763 1163
Size: 573 1027 4962
Fragments arranged by size:
4962 1027 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4012 4773 5801 1540
Size: 275 186 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 3026 3509 4623 4708 5779 2517
Size: 509 483 1114 85 1071 3300
Fragments arranged by size:
3300 1114 1071 509 483 85
BsmI GAATG_Cn'
Cuts at: 2044 2497 4073 4166 2044
Size: 453 1576 93 4440
Fragments arranged by size:
4440 1576 453 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2517 3026 3509 3881 4623 4708
Size: 399 484 243 509 483 372 742 85
Cuts at: 4708 5779 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 509 484 483 399 372
243 85
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 3748 4451 6181 3748
Size: 703 1730 4129
Fragments arranged by size:
4129 1730 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3917 844
Size: 3073 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5057 5231 65
Size: 1183 3809 174 1396
Fragments arranged by size:
3809 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5206 5393 1700
Size: 110 3396 187 2869
Fragments arranged by size:
3396 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6562
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 3602 3602
Size: 6562
BstYI r'GATC_y
Cuts at: 1972 2018 3376 3707 3892 4259 4644 4661 6113
Size: 46 1358 331 185 367 385 17 1452
Cuts at: 6113 6199 6211 6557 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1358 385 367 346 331 185
86 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 4078 4872 4892 4078
Size: 794 20 5748
Fragments arranged by size:
5748 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3315 3866 3315
Size: 551 6011
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2736 4009
Size: 54 1425 50 275 18 373 533 1273
Cuts at: 4009 4898 6527 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1273 889 533 373 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2427 2719 3601 3677 3752 4492 1353
Size: 909 165 292 882 76 75 740 3423
Fragments arranged by size:
3423 909 882 740 292 165 76 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5149 5523 5669 387
Size: 1064 138 3560 374 146 1280
Fragments arranged by size:
3560 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3024 3024
Size: 6562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2286 2519 3246 3324 4625 5222
Size: 393 452 202 233 727 78 1301 597
Cuts at: 5222 6009 6277 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1301 787 727 597 452 393 268
233 202 78
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 6562
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 2302 5709 1678
Size: 624 3407 2531
Fragments arranged by size:
3407 2531 624
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2340 2372 2465 2497 2539
Size: 32 135 32 768 32 93 32 42
Cuts at: 2539 2571 2738 2770 5203 5235 5277 5309 1373
Size: 32 167 32 2433 32 42 32 2626
Fragments arranged by size:
2626 2433 768 167 135 93 42 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3906 4152 669
Size: 988 281 1968 246 3079
Fragments arranged by size:
3079 1968 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6562
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 3191 5676 5860 1305
Size: 780 1106 2485 184 2007
Fragments arranged by size:
2485 2007 1106 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2205 2738 10
Size: 54 1425 343 373 533 3834
Fragments arranged by size:
3834 1425 533 373 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 4520 4879 5038 518
Size: 648 1527 1827 359 159 2042
Fragments arranged by size:
2042 1827 1527 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2768 3978 4655 5803 6048 1606
Size: 963 199 1210 677 1148 245 2120
Fragments arranged by size:
2120 1210 1148 963 677 245 199
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6010
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NheI G'CTAG_C
Cuts at: 1052 3184 1052
Size: 2132 4430
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 2972 3920 5465 1132
Size: 97 1743 948 1545 2229
Fragments arranged by size:
2229 1743 1545 948 97
PasI CC'CwG_GG
Cuts at: 1348 2993 1348
Size: 1645 4917
PciI A'CATG_T
Cuts at: 2968 5461 2968
Size: 2493 4069
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3625 3657 3625
Size: 32 6530
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2569 2768 2569
Size: 199 6363
SacI G_AGCT'C
Cuts at: 3026 3026
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SapI GCTCTTCn'nnn_
Cuts at: 2262 2262
Size: 6562
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 4810 6522 4810
Size: 1712 4850
SfcI C'TryA_G
Cuts at: 826 1045 2136 2142 3027 3910 5045 5726 5917
Size: 219 1091 6 885 883 1135 681 191
Cuts at: 5917 826
Size: 1471
Fragments arranged by size:
1471 1135 1091 885 883 681 219 191
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4666 5567 5829 820
Size: 197 926 2723 901 262 1553
Fragments arranged by size:
2723 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 3528 1517
Size: 2011 4551
StyI C'CwwG_G
Cuts at: 513 1065 4001 513
Size: 552 2936 3074
Fragments arranged by size:
3074 2936 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4589 4606 4759 4944 5389 325
Size: 1255 3009 17 153 185 445 1498
Fragments arranged by size:
3009 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4808 6520 96
Size: 274 80 33 51 1443 2831 1712 138
Fragments arranged by size:
2831 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6522 6554 6522
Size: 32 6530
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3082 3082
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 2097 3123 3393 4691 2097
Size: 1026 270 1298 3968
Fragments arranged by size:
3968 1298 1026 270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI