Restriction map for
pFN21AB8615
(Circular) (Six-base) MAPSORT of: pFN21AB8615.seq Check: 5463 from: 1 to: 6169
pFN21AB8615 6169 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2846 3009 4232 5616 5884 1632
Size: 1214 163 1223 1384 268 1917
Fragments arranged by size:
1917 1384 1223 1214 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 2347 2658 4898 2347
Size: 311 2240 3618
Fragments arranged by size:
3618 2240 311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2918 3351 1184
Size: 1734 433 4002
Fragments arranged by size:
4002 1734 433
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 6137
AlwNI CAG_nnn'CTG
Cuts at: 2360 3912 5484 2360
Size: 1552 1572 3045
Fragments arranged by size:
3045 1572 1552
ApaI G_GGCC'C
Cuts at: 2286 2286
Size: 6169
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2606 4723 160
Size: 872 1574 2117 1606
Fragments arranged by size:
2117 1606 1574 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 3290 3494 1870
Size: 73 843 504 204 4545
Fragments arranged by size:
4545 843 504 204 73
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3251 3490 4945 618
Size: 325 217 627 1464 239 1455 1842
Fragments arranged by size:
1842 1464 1455 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2286 2787 3488 1391
Size: 895 501 701 4072
Fragments arranged by size:
4072 895 701 501
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3217 3290 928
Size: 971 1318 73 3807
Fragments arranged by size:
3807 1318 971 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3327 3361 4360 4394 1903
Size: 34 1390 34 999 34 3678
Fragments arranged by size:
3678 1390 999 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4780 136
Size: 107 122 71 631 605 3108 1525
Fragments arranged by size:
3108 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2043 2400 6164 2043
Size: 357 3764 2048
Fragments arranged by size:
3764 2048 357
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2286 4230 5386 1790
Size: 496 1944 1156 2573
Fragments arranged by size:
2573 1944 1156 496
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3188 3900 4858
Size: 416 53 83 399 24 1771 712 958
Cuts at: 4858 442
Size: 1753
Fragments arranged by size:
1771 1753 958 712 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4829 1239
Size: 3590 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2866 4294 5159 5457 2432
Size: 434 1428 865 298 3144
Fragments arranged by size:
3144 1428 865 434 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3960 493
Size: 711 2756 2702
Fragments arranged by size:
2756 2702 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5944
Fragments arranged by size:
5944 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2795 1186
Size: 1609 4560
BseYI C'CCAG_C
Cuts at: 3029 5372 5868 3029
Size: 2343 496 3330
Fragments arranged by size:
3330 2343 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2470 1163
Size: 573 734 4862
Fragments arranged by size:
4862 734 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2787 4230 4315 5386 2103
Size: 684 1443 85 1071 2886
Fragments arranged by size:
2886 1443 1071 684 85
BsmI GAATG_Cn'
Cuts at: 2923 3680 3773 2923
Size: 757 93 5319
Fragments arranged by size:
5319 757 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2286 2787 3488 4230 4315 5386
Size: 399 313 183 501 701 742 85 1071
Cuts at: 5386 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 701 501 399 313 183
85
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 2514 4058 5788 2514
Size: 1544 1730 2895
Fragments arranged by size:
2895 1730 1544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 844
Size: 2680 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4056 1394
Size: 429 2233 3507
Fragments arranged by size:
3507 2233 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3061 4813 5000 1700
Size: 110 1251 1752 187 2869
Fragments arranged by size:
2869 1752 1251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2388 3406 4229 5241 2388
Size: 1018 823 1012 3316
Fragments arranged by size:
3316 1018 1012 823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3399 794
Size: 2605 3564
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 2710
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2043 2400 3080 3499 3866 4251 4268 5720
Size: 71 357 680 419 367 385 17 1452
Cuts at: 5720 5806 5818 6164 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 680 419 385 367 357 346
86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 6169
BtgI C'CryG_G
Cuts at: 513 1065 2004 2640 513
Size: 552 939 636 4042
Fragments arranged by size:
4042 939 636 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 3163 3685 4479 4499 2161
Size: 1002 522 794 20 3831
Fragments arranged by size:
3831 1002 794 522 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 2435 2435
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2727 3063 3465
Size: 54 1425 50 275 18 897 336 402
Cuts at: 3465 3616 4505 6134 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 897 889 402 336 275 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4099 1353
Size: 2746 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 2785
Size: 6169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2846 3009 4232 4829 5616 5884 1239
Size: 393 1214 163 1223 597 787 268 1524
Fragments arranged by size:
1524 1223 1214 787 597 393 268 163
EcoNI CCTnn'n_nnAGG
Cuts at: 2277 2277
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2282 2283 3295 3357 2282
Size: 1 1012 62 5094
Fragments arranged by size:
5094 1012 62 1
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
EcoRV GAT'ATC
Cuts at: 2507 2507
Size: 6169
FspI TGC'GCA
Cuts at: 2922 4675 2922
Size: 1753 4416
FspAI rTGC'GCAy
Cuts at: 2922 2922
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 5316 1678
Size: 3638 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 2496 2528 2644
Size: 32 135 32 761 32 131 32 116
Cuts at: 2644 2676 3091 3123 4810 4842 4884 4916 1373
Size: 32 415 32 1687 32 42 32 2626
Fragments arranged by size:
2626 1687 761 415 135 131 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 5283 5467 1305
Size: 823 3155 184 2007
Fragments arranged by size:
3155 2007 823 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4347
Fragments arranged by size:
4347 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2028 4127 4486 4645 518
Size: 648 862 2099 359 159 2042
Fragments arranged by size:
2099 2042 862 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2725 2824 3585 4262 5410 5655 1606
Size: 1119 99 761 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 761 677 245 99
NaeI GCC'GGC
Cuts at: 3063 3063
Size: 6169
NcoI C'CATG_G
Cuts at: 513 1065 2004 2640 513
Size: 552 939 636 4042
Fragments arranged by size:
4042 939 636 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NgoMIV G'CCGG_C
Cuts at: 3061 3061
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2282 2282
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2292 2897 3340 3521 830
Size: 1462 605 443 181 3478
Fragments arranged by size:
3478 1462 605 443 181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2725 2824 2725
Size: 99 6070
SacI G_AGCT'C
Cuts at: 2787 2787
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2288 2893 3336 3517 4652 5333 5524
Size: 219 1243 605 443 181 1135 681 191
Cuts at: 5524 826
Size: 1471
Fragments arranged by size:
1471 1243 1135 681 605 443 219 191
181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2881 4273 5174 5436 820
Size: 197 926 468 470 1392 901 262 1553
Fragments arranged by size:
1553 1392 926 901 470 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 2455 4093 5
Size: 47 2403 1638 2081
Fragments arranged by size:
2403 2081 1638 47
StuI AGG'CCT
Cuts at: 1517 2272 1517
Size: 755 5414
StyI C'CwwG_G
Cuts at: 513 1065 2004 2640 3273 3608 513
Size: 552 939 636 633 335 3074
Fragments arranged by size:
3074 939 636 633 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4196 4213 4366 4551 4996 325
Size: 1255 2616 17 153 185 445 1498
Fragments arranged by size:
2616 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4415 6127 96
Size: 274 80 33 51 1443 2438 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 6137
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 4298 4298
Size: 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I FalI FseI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI