Restriction map for
pFN21AB8603
(Circular) (Six-base) MAPSORT of: pFN21AB8603.seq Check: 2961 from: 1 to: 5941
pFN21AB8603 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 2115 3262 2115
Size: 1147 4794
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 4004 5388 5656 1632
Size: 1166 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 1166 268
AfeI AGC'GCT
Cuts at: 3119 3119
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 2294 820
Size: 197 1277 4467
Fragments arranged by size:
4467 1277 197
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 3006
Size: 32 5909
AlwNI CAG_nnn'CTG
Cuts at: 3194 3684 5256 3194
Size: 490 1572 3879
Fragments arranged by size:
3879 1572 490
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2009 2201 3250 3476 3628 5737 1493
Size: 516 192 1049 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 516 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2937 3266 1870
Size: 73 994 329 4545
Fragments arranged by size:
4545 994 329 73
BamHI G'GATC_C
Cuts at: 2555 3271 3638 2555
Size: 716 367 4858
Fragments arranged by size:
4858 716 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 2980 3262 4717 618
Size: 325 217 627 328 865 282 1455 1842
Fragments arranged by size:
1842 1455 865 627 328 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2931 3260 1391
Size: 1540 329 4072
Fragments arranged by size:
4072 1540 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2883 928
Size: 971 984 3986
Fragments arranged by size:
3986 984 971
BbvCI CC'TCA_GC
Cuts at: 2719 2719
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BfrBI ATG'CAT
Cuts at: 2381 2381
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2315 3358 2315
Size: 1043 4898
Bme1580I G_kGCm'C
Cuts at: 1790 2985 4002 5158 1790
Size: 1195 1017 1156 2573
Fragments arranged by size:
2573 1195 1156 1017
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2960 3672 4630
Size: 416 53 83 399 24 1543 712 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1543 958 712 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4601 1239
Size: 3362 2579
Bpu10I CC'TnA_GC
Cuts at: 2719 2719
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 3250 4066 4931 5229 2153
Size: 1097 816 865 298 2865
Fragments arranged by size:
2865 1097 865 816 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2676 4374 4772 5046 5193 5604 2676
Size: 1698 398 274 147 411 3013
Fragments arranged by size:
3013 1698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 3191 3221 1178
Size: 30 165 30 1362 30 396 30 3898
Fragments arranged by size:
3898 1362 396 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 2877 1186
Size: 1582 109 4250
Fragments arranged by size:
4250 1582 109
BseYI C'CCAG_C
Cuts at: 5144 5640 5144
Size: 496 5445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2217 1163
Size: 573 481 4887
Fragments arranged by size:
4887 573 481
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2948 4002 4087 5158 2948
Size: 1054 85 1071 3731
Fragments arranged by size:
3731 1071 1054 85
BsmI GAATG_Cn'
Cuts at: 2070 2857 3452 3545 2070
Size: 787 595 93 4466
Fragments arranged by size:
4466 787 595 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2931 2948 2985 3260 4002 4087 5158
Size: 399 1141 17 37 275 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1141 1071 742 399 275 85 37
17
BspEI T'CCGG_A
Cuts at: 2676 5604 2676
Size: 2928 3013
BspHI T'CATG_A
Cuts at: 2916 3180 3830 5560 2916
Size: 264 650 1730 3297
Fragments arranged by size:
3297 1730 650 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 3296 844
Size: 1292 1160 3489
Fragments arranged by size:
3489 1292 1160
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2387 794
Size: 1593 4348
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2042 2555 2758 3271 3638 4023 4040 5492
Size: 70 513 203 513 367 385 17 1452
Cuts at: 5492 5578 5590 5936 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 513 513 385 367 346 203
86 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 3457 4251 4271 2463
Size: 994 794 20 4133
Fragments arranged by size:
4133 994 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3388 4277 5906
Size: 54 1425 50 275 18 1558 889 1629
Cuts at: 5906 8
Size: 43
Fragments arranged by size:
1629 1558 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2532 3871 1353
Size: 1160 19 1339 3423
Fragments arranged by size:
3423 1339 1160 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 4004 4601 5388 5656 1239
Size: 393 1166 1206 597 787 268 1524
Fragments arranged by size:
1524 1206 1166 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2201 3250 3628 2201
Size: 1049 378 4514
Fragments arranged by size:
4514 1049 378
EcoRV GAT'ATC
Cuts at: 2791 2791
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 3121 5088 1678
Size: 1443 1967 2531
Fragments arranged by size:
2531 1967 1443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2765 2797 3092
Size: 32 135 32 736 32 425 32 295
Cuts at: 3092 3124 4582 4614 4656 4688 1373
Size: 32 1458 32 42 32 2626
Fragments arranged by size:
2626 1458 736 425 295 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3067 3285 3531 669
Size: 988 281 1129 218 246 3079
Fragments arranged by size:
3079 1129 988 281 246 218
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 2119 3266 2119
Size: 1147 4794
MfeI C'AATT_G
Cuts at: 3169 3540 3169
Size: 371 5570
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 3040 5055 5239 1305
Size: 817 918 2015 184 2007
Fragments arranged by size:
2015 2007 918 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3899 4258 4417 518
Size: 648 1314 1419 359 159 2042
Fragments arranged by size:
2042 1419 1314 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3357 4034 5182 5427 1606
Size: 1751 677 1148 245 2120
Fragments arranged by size:
2120 1751 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NsiI A_TGCA'T
Cuts at: 2383 2383
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 2804 3511 2804
Size: 707 5234
PstI C_TGCA'G
Cuts at: 830 2266 3293 830
Size: 1436 1027 3478
Fragments arranged by size:
3478 1436 1027
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 2532 2513
Size: 19 5922
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SexAI A'CCwGG_T
Cuts at: 3054 3054
Size: 5941
SfcI C'TryA_G
Cuts at: 826 1045 2262 2564 2794 3289 4424 5105 5296
Size: 219 1217 302 230 495 1135 681 191
Cuts at: 5296 826
Size: 1471
Fragments arranged by size:
1471 1217 1135 681 495 302 230 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2294 2937 3229 4045 4946
Size: 197 926 189 162 643 292 816 901
Cuts at: 4946 5208 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 816 643 292 262 197
189 162
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 3380 513
Size: 552 939 1376 3074
Fragments arranged by size:
3074 1376 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 2937 1943
Size: 994 4947
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 2427 2688 4070 2427
Size: 261 1382 4298
Fragments arranged by size:
4298 1382 261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI