Restriction map for
pFN21AB8602
(Circular) (Six-base) MAPSORT of: pFN21AB8602.seq Check: 2063 from: 1 to: 5341
pFN21AB8602 5341 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2251 2363 3404 4788 5056 1632
Size: 593 26 112 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1041 593 268 112 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 2177 1166
Size: 1011 4330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 2253 2341 3084 4656 2253
Size: 88 743 1572 2938
Fragments arranged by size:
2938 1572 743 88
ApaI G_GGCC'C
Cuts at: 2613 2613
Size: 5341
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2273 2662 4117 618
Size: 325 217 627 405 81 389 1455 1842
Fragments arranged by size:
1842 1455 627 405 389 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2613 2660 1391
Size: 1222 47 4072
Fragments arranged by size:
4072 1222 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BbvCI CC'TCA_GC
Cuts at: 2411 2411
Size: 5341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2153 3233 4443 2153
Size: 1080 1210 3051
Fragments arranged by size:
3051 1210 1080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2617 3952 136
Size: 107 122 71 631 605 945 1335 1525
Fragments arranged by size:
1525 1335 945 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5336 2214
Size: 3122 2219
BlpI GC'TnA_GC
Cuts at: 2331 2758 2331
Size: 427 4914
Bme1580I G_kGCm'C
Cuts at: 1790 2613 3402 4558 1790
Size: 823 789 1156 2573
Fragments arranged by size:
2573 1156 823 789
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 4001 1239
Size: 1133 1629 2579
Fragments arranged by size:
2579 1629 1133
Bpu10I CC'TnA_GC
Cuts at: 2411 2411
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2309 3466 4331 4629 2231
Size: 78 1157 865 298 2943
Fragments arranged by size:
2943 1157 865 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3132 493
Size: 711 918 1010 2702
Fragments arranged by size:
2702 1010 918 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2372 2576 1186
Size: 1183 3 204 3951
Fragments arranged by size:
3951 1183 204 3
BseYI C'CCAG_C
Cuts at: 2028 2045 4544 5040 2028
Size: 17 2499 496 2329
Fragments arranged by size:
2499 2329 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 1163
Size: 573 612 4156
Fragments arranged by size:
4156 612 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 3487 4558 3402
Size: 85 1071 4185
Fragments arranged by size:
4185 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2613 2660 3402 3487 4558 1391
Size: 399 823 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 823 742 399 85 47
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 3228 1394
Size: 429 599 806 3507
Fragments arranged by size:
3507 806 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 2428 1184
Size: 1244 4097
BstYI r'GATC_y
Cuts at: 1972 2214 2671 3038 3423 3440 4892 4978 4990
Size: 242 457 367 385 17 1452 86 12
Cuts at: 4990 5336 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 457 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 513
Size: 552 939 87 3763
Fragments arranged by size:
3763 939 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2342 2857 3651 3671 2342
Size: 515 794 20 4012
Fragments arranged by size:
4012 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2163 2198 529
Size: 35 1599 35 3672
Fragments arranged by size:
3672 1599 35 35
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DraIII CAC_nnn'GTG
Cuts at: 2509 2509
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2230 2490 2788
Size: 54 1425 50 275 18 400 260 298
Cuts at: 2788 3677 5306 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 400 298 275 260 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 3271 1353
Size: 765 1153 3423
Fragments arranged by size:
3423 1153 765
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2251 2363 2372 3404 4001 4788
Size: 393 593 26 112 9 1032 597 787
Cuts at: 4788 5056 1239
Size: 268 1524
Fragments arranged by size:
1524 1032 787 597 593 393 268 112
26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2414 2354
Size: 60 5281
EcoO109I rG'GnC_Cy
Cuts at: 2022 2347 2609 2022
Size: 325 262 4754
Fragments arranged by size:
4754 325 262
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2149 2335 2122
Size: 27 186 5128
Fragments arranged by size:
5128 186 27
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 2207 748
Size: 1459 3882
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2493 4455 4639 1305
Size: 1188 1962 184 2007
Fragments arranged by size:
2007 1962 1188 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2232 10
Size: 54 1425 343 400 3119
Fragments arranged by size:
3119 1425 400 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3299 3658 3817 518
Size: 648 1011 1122 359 159 2042
Fragments arranged by size:
2042 1122 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2228 2591 2757 3434 4582 4827 1606
Size: 622 363 166 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 622 363 245 166
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 2034 1348
Size: 686 4655
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PfoI T'CCnGG_A
Cuts at: 2436 2436
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2022 2347 2022
Size: 325 5016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2609 2609
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2550 2693 830
Size: 1720 143 3478
Fragments arranged by size:
3478 1720 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2228 2591 2228
Size: 363 4978
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SapI GCTCTTCn'nnn_
Cuts at: 2118 2118
Size: 5341
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SexAI A'CCwGG_T
Cuts at: 2505 2505
Size: 5341
SfcI C'TryA_G
Cuts at: 826 1045 2546 2689 3824 4505 4696 826
Size: 219 1501 143 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2324 3445 4346 4608 820
Size: 197 926 267 114 1121 901 262 1553
Fragments arranged by size:
1553 1121 926 901 267 262 197 114
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2780 513
Size: 552 939 776 3074
Fragments arranged by size:
3074 939 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 2382 3368 3385 3538 3723 4168
Size: 1255 600 202 986 17 153 185 445
Cuts at: 4168 325
Size: 1498
Fragments arranged by size:
1498 1255 986 600 445 202 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 2132 3470 2132
Size: 1338 4003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI