Restriction map for
pFN21AB8595
(Circular) (Six-base) MAPSORT of: pFN21AB8595.seq Check: 3908 from: 1 to: 11311
pFN21AB8595 11311 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3895 3895
Size: 11311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3664 6703 7291 9121
Size: 53 83 186 936 2128 3039 588 1830
Cuts at: 9121 278
Size: 2468
Fragments arranged by size:
3039 2468 2128 1830 936 588 186 83
53
AccI GT'mk_AC
Cuts at: 1937 8654 1937
Size: 6717 4594
Acc65I G'GTAC_C
Cuts at: 8632 8632
Size: 11311
AclI AA'CG_TT
Cuts at: 1563 5305 8538 9438 9811 1563
Size: 3742 3233 900 373 3063
Fragments arranged by size:
3742 3233 3063 900 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2742 2780 2847 3255 3637 5079 5534 5903
Size: 1110 38 67 408 382 1442 455 369
Cuts at: 5903 5959 6768 7551 9374 10758 11026 1632
Size: 56 809 783 1823 1384 268 1917
Fragments arranged by size:
1917 1823 1442 1384 1110 809 783 455
408 382 369 268 67 56 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11114
AflIII A'CryG_T
Cuts at: 1203 3988 10210 11187 1203
Size: 2785 6222 977 1327
Fragments arranged by size:
6222 2785 1327 977
AgeI A'CCGG_T
Cuts at: 10142 10142
Size: 11311
AhdI GACnn_n'nnGTC
Cuts at: 5516 10040 5516
Size: 4524 6787
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3201 1184
Size: 2017 9294
AlwNI CAG_nnn'CTG
Cuts at: 3512 4675 5240 5822 6315 9054 10626 3512
Size: 1163 565 582 493 2739 1572 4197
Fragments arranged by size:
4197 2739 1572 1163 582 565 493
ApaI G_GGCC'C
Cuts at: 7958 7958
Size: 11311
ApaLI G'TGCA_C
Cuts at: 9368 10524 9368
Size: 1156 10155
ApoI r'AATT_y
Cuts at: 1493 2159 2500 2698 2940 3552 3593 3726 4378
Size: 666 341 198 242 612 41 133 652
Cuts at: 4378 4530 4583 5063 6424 7148 8086 8620 8846
Size: 152 53 480 1361 724 938 534 226
Cuts at: 8846 8998 11107 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1361 938 724 666 652 612
534 480 341 242 226 198 152 152
133 53 41
AseI AT'TA_AT
Cuts at: 160 1032 9865 160
Size: 872 8833 1606
Fragments arranged by size:
8833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11311
AvaI C'yCGr_G
Cuts at: 1870 1943 8636 1870
Size: 73 6693 4545
Fragments arranged by size:
6693 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4283 4316 4283
Size: 33 11278
BamHI G'GATC_C
Cuts at: 8641 9008 8641
Size: 367 10944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 8632 10087 618
Size: 325 217 627 6845 1455 1842
Fragments arranged by size:
6845 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 7958 8630 1391
Size: 6567 672 4072
Fragments arranged by size:
6567 4072 672
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 6094 7619 928
Size: 971 315 3880 1525 4620
Fragments arranged by size:
4620 3880 1525 971 315
BbvCI CC'TCA_GC
Cuts at: 5841 5841
Size: 11311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3536 3570 9502 9536 1903
Size: 34 1599 34 5932 34 3678
Fragments arranged by size:
5932 3678 1599 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3117 9203 10413 3117
Size: 6086 1210 4015
Fragments arranged by size:
6086 4015 1210
BclI T'GATC_A
Cuts at: 1548 2902 5068 5632 5692 1548
Size: 1354 2166 564 60 7167
Fragments arranged by size:
7167 2166 1354 564 60
BfrBI ATG'CAT
Cuts at: 2524 6859 7253 2524
Size: 4335 394 6582
Fragments arranged by size:
6582 4335 394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6740 9922 136
Size: 107 122 71 631 605 5068 3182 1525
Fragments arranged by size:
5068 3182 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2740 11306 2740
Size: 8566 2745
BlpI GC'TnA_GC
Cuts at: 8728 8728
Size: 11311
Bme1580I G_kGCm'C
Cuts at: 1790 3502 7958 9372 10528 1790
Size: 1712 4456 1414 1156 2573
Fragments arranged by size:
4456 2573 1712 1414 1156
BmgBI CAC'GTC
Cuts at: 1318 3029 7792 1318
Size: 1711 4763 4837
Fragments arranged by size:
4837 4763 1711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6554 7163 9042
Size: 416 53 83 399 24 5137 609 1879
Cuts at: 9042 10000 442
Size: 958 1753
Fragments arranged by size:
5137 1879 1753 958 609 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5379 1056
Size: 4323 6988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5618 5910 5618
Size: 292 11019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2600 2840 3087 5166 6648 7040 9971
Size: 959 402 240 247 2079 1482 392 2931
Cuts at: 9971 1239
Size: 2579
Fragments arranged by size:
2931 2579 2079 1482 959 402 392 247
240
Bpu10I CC'TnA_GC
Cuts at: 5841 5841
Size: 11311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 6812 9436 10301 10599 2624
Size: 4188 2624 865 298 3336
Fragments arranged by size:
4188 3336 2624 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4985 6489 8584 9974 882
Size: 617 3486 1504 2095 1390 2219
Fragments arranged by size:
3486 2219 2095 1504 1390 617
BsaAI yAC'GTr
Cuts at: 493 1204 6161 9102 493
Size: 711 4957 2941 2702
Fragments arranged by size:
4957 2941 2702 711
BsaBI GATnn'nnATC
Cuts at: 7853 11305 7853
Size: 3452 7859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3661 6700 7288 9118
Size: 53 83 186 936 2128 3039 588 1830
Cuts at: 9118 9500 10135 275
Size: 382 635 1451
Fragments arranged by size:
3039 2128 1830 1451 936 635 588 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 9744 10142 10416 10563 10974 9744
Size: 398 274 147 411 10081
Fragments arranged by size:
10081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5207 5237 1178
Size: 30 165 30 3804 30 7252
Fragments arranged by size:
7252 3804 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 4928 5114 5220 5886 8307 1186
Size: 1657 2085 186 106 666 2421 4190
Fragments arranged by size:
4190 2421 2085 1657 666 186 106
BseYI C'CCAG_C
Cuts at: 2818 7073 7304 10514 11010 2818
Size: 4255 231 3210 496 3119
Fragments arranged by size:
4255 3210 3119 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2474 8328 8544 1163
Size: 573 738 5854 216 3930
Fragments arranged by size:
5854 3930 738 573 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8761 9522 10550 1540
Size: 275 186 6760 761 1028 2301
Fragments arranged by size:
6760 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5281 6052 7197 9372 9457 10528 5281
Size: 771 1145 2175 85 1071 6064
Fragments arranged by size:
6064 2175 1145 1071 771 85
BsmI GAATG_Cn'
Cuts at: 3154 4365 5263 6861 7526 8822 8915 3154
Size: 1211 898 1598 665 1296 93 5550
Fragments arranged by size:
5550 1598 1296 1211 898 665 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2024 2024
Size: 11311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3502 5281 6052 7197 7958 8630 9372
Size: 399 1712 1779 771 1145 761 672 742
Cuts at: 9372 9457 10528 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1779 1712 1145 1071 771 761 742
672 399 85
BspEI T'CCGG_A
Cuts at: 10974 10974
Size: 11311
BspHI T'CATG_A
Cuts at: 2113 2298 7120 7296 8487 9200 10930 2113
Size: 185 4822 176 1191 713 1730 2494
Fragments arranged by size:
4822 2494 1730 1191 713 185 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3895 8666 844
Size: 3051 4771 3489
Fragments arranged by size:
4771 3489 3051
BsrBI CCG'CTC
Cuts at: 1394 1823 9198 1394
Size: 429 7375 3507
Fragments arranged by size:
7375 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3525 3537 6255 6801 7788 9806 9980
Size: 1183 2277 12 2718 546 987 2018 174
Cuts at: 9980 65
Size: 1396
Fragments arranged by size:
2718 2277 2018 1396 1183 987 546 174
12
BsrFI r'CCGG_y
Cuts at: 1700 1810 9955 10142 1700
Size: 110 8145 187 2869
Fragments arranged by size:
8145 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4168 4427 5967 5992 96
Size: 4072 259 1540 25 5415
Fragments arranged by size:
5415 4072 1540 259 25
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11311
BssSI C'ACGA_G
Cuts at: 8137 9371 10383 8137
Size: 1234 1012 9065
Fragments arranged by size:
9065 1234 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3260 4709 5249 5258 7220 794
Size: 2466 1449 540 9 1962 4885
Fragments arranged by size:
4885 2466 1962 1449 540 9
BstBI TT'CG_AA
Cuts at: 8413 10115 8413
Size: 1702 9609
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11311
BstXI CCAn_nnnn'nTGG
Cuts at: 3765 6294 6377 6765 8012 3765
Size: 2529 83 388 1247 7064
Fragments arranged by size:
7064 2529 1247 388 83
BstYI r'GATC_y
Cuts at: 1972 2740 5680 5914 6366 6754 8641 9008 9393
Size: 768 2940 234 452 388 1887 367 385
Cuts at: 9393 9410 10862 10948 10960 11306 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2940 1977 1887 1452 768 452 388 385
367 346 234 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3111 3111
Size: 11311
BtgI C'CryG_G
Cuts at: 513 1065 2004 8040 513
Size: 552 939 6036 3784
Fragments arranged by size:
6036 3784 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10572
Fragments arranged by size:
10572 683 56
BtsI GCAGTG_nn'
Cuts at: 2385 4749 5823 6770 8827 9621 9641 2385
Size: 2364 1074 947 2057 794 20 4055
Fragments arranged by size:
4055 2364 2057 1074 947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11276
DraI TTT'AAA
Cuts at: 3418 8615 3418
Size: 5197 6114
DraIII CAC_nnn'GTG
Cuts at: 4156 4156
Size: 11311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6275 10318 809
Size: 507 4959 4043 1802
Fragments arranged by size:
4959 4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4024 4231 7450
Size: 54 1425 50 275 18 2194 207 3219
Cuts at: 7450 8758 9647 11276 8
Size: 1308 889 1629 43
Fragments arranged by size:
3219 2194 1629 1425 1308 889 275 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8758 1537
Size: 275 6946 4090
Fragments arranged by size:
6946 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2300 2925 3422 5082 7885 7890 9241
Size: 824 123 625 497 1660 2803 5 1351
Cuts at: 9241 1353
Size: 3423
Fragments arranged by size:
3423 2803 1660 1351 824 625 497 123
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9898 10272 10418 387
Size: 1064 138 8309 374 146 1280
Fragments arranged by size:
8309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2600 2742 2780 2840 2847 3087
Size: 393 566 402 142 38 60 7 240
Cuts at: 3087 3255 3637 5079 5166 5534 5903 5959 6648
Size: 168 382 1442 87 368 369 56 689
Cuts at: 6648 6768 7040 7551 9374 9971 10758 11026 1239
Size: 120 272 511 1823 597 787 268 1524
Fragments arranged by size:
1823 1524 1442 787 689 597 566 511
402 393 382 369 368 272 268 240
168 142 120 87 60 56 38 7
EcoNI CCTnn'n_nnAGG
Cuts at: 7688 7688
Size: 11311
EcoO109I rG'GnC_Cy
Cuts at: 2822 6392 7954 2822
Size: 3570 1562 6179
Fragments arranged by size:
6179 3570 1562
EcoRI G'AATT_C
Cuts at: 8620 8998 8620
Size: 378 10933
EcoRV GAT'ATC
Cuts at: 5035 5035
Size: 11311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4779 6218 7153 7525 7556 8570 4779
Size: 1439 935 372 31 1014 7520
Fragments arranged by size:
7520 1439 1014 935 372 31
FspI TGC'GCA
Cuts at: 9817 9817
Size: 11311
HaeII r_GCGC'y
Cuts at: 1678 10458 1678
Size: 8780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4540 4572 4774 4806 5207
Size: 32 135 32 2968 32 202 32 401
Cuts at: 5207 5239 5502 5534 6236 6268 7278 7310 7846
Size: 32 263 32 702 32 1010 32 536
Cuts at: 7846 7878 9952 9984 10026 10058 1373
Size: 32 2074 32 42 32 2626
Fragments arranged by size:
2968 2626 2074 1010 702 536 401 263
202 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4522 6599 8569 8655 8901 669
Size: 988 281 2584 2077 1970 86 246 3079
Fragments arranged by size:
3079 2584 2077 1970 988 281 246 86
HindIII A'AGCT_T
Cuts at: 748 3311 5438 748
Size: 2563 2127 6621
Fragments arranged by size:
6621 2563 2127
HpaI GTT'AAC
Cuts at: 8901 8901
Size: 11311
KpnI G_GTAC'C
Cuts at: 8636 8636
Size: 11311
MfeI C'AATT_G
Cuts at: 2271 2871 2929 4659 8910 2271
Size: 600 58 1730 4251 4672
Fragments arranged by size:
4672 4251 1730 600 58
MluI A'CGCG_T
Cuts at: 11187 11187
Size: 11311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2227 4855 6445 6568 6846 7309 7952 10425
Size: 922 2628 1590 123 278 463 643 2473
Cuts at: 10425 10609 1305
Size: 184 2007
Fragments arranged by size:
2628 2473 2007 1590 922 643 463 278
184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4233 10
Size: 54 1425 343 2401 7088
Fragments arranged by size:
7088 2401 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 3530 3683 4269 7209 8010 9269
Size: 648 2053 311 153 586 2940 801 1259
Cuts at: 9269 9628 9787 518
Size: 359 159 2042
Fragments arranged by size:
2940 2053 2042 1259 801 648 586 359
311 159 153
MspA1I CmG'CkG
Cuts at: 1606 3409 3635 6080 8727 9404 10552 10797 1606
Size: 1803 226 2445 2647 677 1148 245 2120
Fragments arranged by size:
2647 2445 2120 1803 1148 677 245 226
NcoI C'CATG_G
Cuts at: 513 1065 2004 8040 513
Size: 552 939 6036 3784
Fragments arranged by size:
6036 3784 939 552
NdeI CA'TA_TG
Cuts at: 387 4801 5245 5956 7602 387
Size: 4414 444 711 1646 4096
Fragments arranged by size:
4414 4096 1646 711 444
NheI G'CTAG_C
Cuts at: 1052 5375 1052
Size: 4323 6988
NotI GC'GGCC_GC
Cuts at: 8758 8758
Size: 11311
NsiI A_TGCA'T
Cuts at: 2526 6861 7255 2526
Size: 4335 394 6582
Fragments arranged by size:
6582 4335 394
NspI r_CATG'y
Cuts at: 1132 1229 2231 2524 3490 3709 3992 4175 7964
Size: 97 1002 293 966 219 283 183 3789
Cuts at: 7964 8063 8231 8669 10214 1132
Size: 99 168 438 1545 2229
Fragments arranged by size:
3789 2229 1545 1002 966 438 293 283
219 183 168 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11311
PciI A'CATG_T
Cuts at: 3988 10210 3988
Size: 6222 5089
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 4004 4159 5761 7063 1108
Size: 1450 1446 155 1602 1302 5356
Fragments arranged by size:
5356 1602 1450 1446 1302 155
PfoI T'CCnGG_A
Cuts at: 2616 5143 2616
Size: 2527 8784
PmeI GTTT'AAAC
Cuts at: 8615 8615
Size: 11311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6381 6413 9380 9412 10893 10925 6381
Size: 32 2967 32 1481 32 6767
Fragments arranged by size:
6767 2967 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2822 2822
Size: 11311
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11311
PsiI TTA'TAA
Cuts at: 2070 2592 4604 7143 8881 2070
Size: 522 2012 2539 1738 4500
Fragments arranged by size:
4500 2539 2012 1738 522
PspOMI G'GGCC_C
Cuts at: 7954 7954
Size: 11311
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6546 6578 7101 7133 6546
Size: 32 523 32 10724
Fragments arranged by size:
10724 523 32 32
PstI C_TGCA'G
Cuts at: 830 2392 8194 8663 830
Size: 1562 5802 469 3478
Fragments arranged by size:
5802 3478 1562 469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11311
PvuII CAG'CTG
Cuts at: 3409 3635 6080 3409
Size: 226 2445 8640
Fragments arranged by size:
8640 2445 226
SalI G'TCGA_C
Cuts at: 1936 8653 1936
Size: 6717 4594
SapI GCTCTTCn'nnn_
Cuts at: 2925 2925
Size: 11311
SbfI CC_TGCA'GG
Cuts at: 8663 8663
Size: 11311
ScaI AGT'ACT
Cuts at: 7528 9559 11271 7528
Size: 2031 1712 7568
Fragments arranged by size:
7568 2031 1712
SexAI A'CCwGG_T
Cuts at: 4152 4152
Size: 11311
SfcI C'TryA_G
Cuts at: 826 1045 2388 3337 3504 5323 7688 8190 8659
Size: 219 1343 949 167 1819 2365 502 469
Cuts at: 8659 9794 10475 10666 826
Size: 1135 681 191 1471
Fragments arranged by size:
2365 1819 1471 1343 1135 949 681 502
469 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11311
SmaI CCC'GGG
Cuts at: 1872 8638 1872
Size: 6766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 6827 9415 10316 10578 820
Size: 197 926 696 4188 2588 901 262 1553
Fragments arranged by size:
4188 2588 1553 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 9102 493
Size: 8609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11311
SphI G_CATG'C
Cuts at: 1132 8231 8669 1132
Size: 7099 438 3774
Fragments arranged by size:
7099 3774 438
SspI AAT'ATT
Cuts at: 5 52 5506 6006 6517 9235 5
Size: 47 5454 500 511 2718 2081
Fragments arranged by size:
5454 2718 2081 511 500 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11311
StyI C'CwwG_G
Cuts at: 513 1065 2004 3627 4258 4525 5660 6261 7096
Size: 552 939 1623 631 267 1135 601 835
Cuts at: 7096 8040 8503 8574 8750 513
Size: 944 463 71 176 3074
Fragments arranged by size:
3074 1623 1135 944 939 835 631 601
552 463 267 176 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5825 6900 7310 9338 9355 9508 9693
Size: 1255 4245 1075 410 2028 17 153 185
Cuts at: 9693 10138 325
Size: 445 1498
Fragments arranged by size:
4245 2028 1498 1255 1075 445 410 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2750 4168 4273
Size: 274 80 33 51 1443 773 1418 105
Cuts at: 4273 4427 5967 5992 7526 9557 11269 96
Size: 154 1540 25 1534 2031 1712 138
Fragments arranged by size:
2031 1712 1540 1534 1443 1418 773 274
154 138 105 80 51 33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4057 4089 4140 4172 5397 5429 11271 11303 4057
Size: 32 51 32 1225 32 5842 32 4065
Fragments arranged by size:
5842 4065 1225 51 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11311
XbaI T'CTAG_A
Cuts at: 3664 6400 8647 3664
Size: 2736 2247 6328
Fragments arranged by size:
6328 2736 2247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7750 7750
Size: 11311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11311
XmaI C'CCGG_G
Cuts at: 1870 8636 1870
Size: 6766 4545
XmnI GAAnn'nnTTC
Cuts at: 2416 6658 9440 2416
Size: 4242 2782 4287
Fragments arranged by size:
4287 4242 2782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3662 6701 7289 9119
Size: 53 83 186 936 2128 3039 588 1830
Cuts at: 9119 276
Size: 2468
Fragments arranged by size:
3039 2468 2128 1830 936 588 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BstZ17I EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI