Restriction map for
pFN21AB8594
(Circular) (Six-base) MAPSORT of: pFN21AB8594.seq Check: 1828 from: 1 to: 6145
pFN21AB8594 6145 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 2483
Size: 6145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2203 3955 278
Size: 53 83 186 936 667 1752 2468
Fragments arranged by size:
2468 1752 936 667 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2158 3488 1937
Size: 221 1330 4594
Fragments arranged by size:
4594 1330 221
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 4208 5592 5860 1632
Size: 525 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 525 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 3233 5044 6021 1203
Size: 2030 1811 977 1327
Fragments arranged by size:
2030 1811 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2037 1184
Size: 853 5292
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 2098 2815 2842 2924 3078 3266 3454 3680
Size: 605 717 27 82 154 188 188 226
Cuts at: 3680 3832 5941 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 717 605 226 188 188 154
152 82 27
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 3470 1870
Size: 73 1527 4545
Fragments arranged by size:
4545 1527 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2284 2317 2284
Size: 33 6112
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 4921 618
Size: 325 217 627 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2533 3464 1391
Size: 1142 931 4072
Fragments arranged by size:
4072 1142 931
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 3123 3219 928
Size: 971 504 720 96 3854
Fragments arranged by size:
3854 971 720 504 96
BbvCI CC'TCA_GC
Cuts at: 2427 2427
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3107 3141 4336 4370 1903
Size: 34 1170 34 1195 34 3678
Fragments arranged by size:
3678 1195 1170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2285 4037 5247 2285
Size: 1752 1210 3183
Fragments arranged by size:
3183 1752 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2985 4756 136
Size: 107 122 71 631 605 1313 1771 1525
Fragments arranged by size:
1771 1525 1313 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 4206 5362 1790
Size: 2416 1156 2573
Fragments arranged by size:
2573 2416 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2585 3876 4834
Size: 416 53 83 399 24 1168 1291 958
Cuts at: 4834 442
Size: 1753
Fragments arranged by size:
1753 1291 1168 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2010 4805 1239
Size: 771 2795 2579
Fragments arranged by size:
2795 2579 771
Bpu10I CC'TnA_GC
Cuts at: 2427 2427
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3069 3191 4270 5135 5433 3069
Size: 122 1079 865 298 3781
Fragments arranged by size:
3781 1079 865 298 122
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2200 3952 4334 4969
Size: 53 83 186 936 667 1752 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1752 1451 936 667 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3223 3253 1178
Size: 30 165 30 1820 30 4070
Fragments arranged by size:
4070 1820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6145
BseYI C'CCAG_C
Cuts at: 3114 5348 5844 3114
Size: 2234 496 3415
Fragments arranged by size:
3415 2234 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5572
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2533 3241 4206 4291 5362 2533
Size: 708 965 85 1071 3316
Fragments arranged by size:
3316 1071 965 708 85
BsmI GAATG_Cn'
Cuts at: 2110 2907 3656 3749 2110
Size: 797 749 93 4506
Fragments arranged by size:
4506 797 749 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2533 3241 3464 4206 4291 5362 1391
Size: 399 743 708 223 742 85 1071 2174
Fragments arranged by size:
2174 1071 743 742 708 399 223 85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 2743 4034 5764 2743
Size: 1291 1730 3124
Fragments arranged by size:
3124 1730 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 2677 3500 844
Size: 1639 194 823 3489
Fragments arranged by size:
3489 1639 823 194
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3103 4640 4814 65
Size: 1183 1855 1537 174 1396
Fragments arranged by size:
1855 1537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 3475 3842 4227 4244 5696 5782 5794 6140
Size: 1503 367 385 17 1452 86 12 346
Cuts at: 6140 1972
Size: 1977
Fragments arranged by size:
1977 1503 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 3359 513
Size: 552 939 292 1063 3299
Fragments arranged by size:
3299 1063 939 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 3661 4455 4475 3424
Size: 237 794 20 5094
Fragments arranged by size:
5094 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 2445 2782 3449 2445
Size: 337 667 5141
Fragments arranged by size:
5141 667 337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3592 4481 6110
Size: 54 1425 50 275 18 1762 889 1629
Cuts at: 6110 8
Size: 43
Fragments arranged by size:
1762 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 2854 2912 2952 4075 1353
Size: 823 678 58 40 1123 3423
Fragments arranged by size:
3423 1123 823 678 58 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2531 2531
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2157 4208 4805 5592 5860 1239
Size: 393 378 147 2051 597 787 268 1524
Fragments arranged by size:
2051 1524 787 597 393 378 268 147
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2208
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2068 2068
Size: 6145
EcoRI G'AATT_C
Cuts at: 3266 3454 3832 3266
Size: 188 378 5579
Fragments arranged by size:
5579 378 188
FspI TGC'GCA
Cuts at: 2906 4651 2906
Size: 1745 4400
FspAI rTGC'GCAy
Cuts at: 2906 2906
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 5292 1678
Size: 3614 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2423 2455 2990
Size: 32 135 32 550 32 269 32 535
Cuts at: 2990 3022 4786 4818 4860 4892 1373
Size: 32 1764 32 42 32 2626
Fragments arranged by size:
2626 1764 550 535 269 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2159 2860 3489 3735 669
Size: 988 281 221 701 629 246 3079
Fragments arranged by size:
3079 988 701 629 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2707 5259 5443 1305
Size: 1402 2552 184 2007
Fragments arranged by size:
2552 2007 1402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2970 4103 4462 4621 518
Size: 648 1120 684 1133 359 159 2042
Fragments arranged by size:
2042 1133 1120 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3561 4238 5386 5631 1606
Size: 1955 677 1148 245 2120
Fragments arranged by size:
2120 1955 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2296 513
Size: 552 939 292 4362
Fragments arranged by size:
4362 939 552 292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3237 3503 5048 1132
Size: 97 2008 266 1545 2229
Fragments arranged by size:
2229 2008 1545 266 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 3233 5044 3233
Size: 1811 4334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2068
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 2649 3715 2649
Size: 1066 5079
PstI C_TGCA'G
Cuts at: 830 2494 3497 830
Size: 1664 1003 3478
Fragments arranged by size:
3478 1664 1003
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
SacI G_AGCT'C
Cuts at: 2533 2533
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 2157 3487 1936
Size: 221 1330 4594
Fragments arranged by size:
4594 1330 221
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 3034 4393 6105 3034
Size: 1359 1712 3074
Fragments arranged by size:
3074 1712 1359
SfcI C'TryA_G
Cuts at: 826 1045 2490 3493 4628 5309 5500 826
Size: 219 1445 1003 1135 681 191 1471
Fragments arranged by size:
1471 1445 1135 1003 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3084 3170 4249 5150 5412 820
Size: 197 926 1141 86 1079 901 262 1553
Fragments arranged by size:
1553 1141 1079 926 901 262 197 86
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 2347 2534 3584 513
Size: 552 939 292 51 187 1050 3074
Fragments arranged by size:
3074 1050 939 552 292 187 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3117 4172 4189 4342 4527 4972 325
Size: 1255 1537 1055 17 153 185 445 1498
Fragments arranged by size:
1537 1498 1255 1055 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3032 4391 6103
Size: 274 80 33 51 1443 1055 1359 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
1712 1443 1359 1055 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6105 6137 6105
Size: 32 6113
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 2525 3481 2525
Size: 956 5189
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2201 3953 276
Size: 53 83 186 936 667 1752 2468
Fragments arranged by size:
2468 1752 936 667 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI