Restriction map for
pFN21AB8592
(Circular) (Six-base) MAPSORT of: pFN21AB8592.seq Check: 8685 from: 1 to: 7072
pFN21AB8592 7072 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4306 4306
Size: 7072
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4882 278
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4415 1937
Size: 2478 4594
Acc65I G'GTAC_C
Cuts at: 4393 4393
Size: 7072
AclI AA'CG_TT
Cuts at: 1563 5199 5572 1563
Size: 3636 373 3063
Fragments arranged by size:
3636 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2646 3314 3459 3768 5135 6519 6787 1632
Size: 1014 668 145 309 1367 1384 268 1917
Fragments arranged by size:
1917 1384 1367 1014 668 309 268 145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6875
AflIII A'CryG_T
Cuts at: 1203 2196 2421 4249 5971 6948 1203
Size: 993 225 1828 1722 977 1327
Fragments arranged by size:
1828 1722 1327 993 977 225
AgeI A'CCGG_T
Cuts at: 5903 5903
Size: 7072
AhdI GACnn_n'nnGTC
Cuts at: 5801 5801
Size: 7072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7072
AlwNI CAG_nnn'CTG
Cuts at: 4815 6387 4815
Size: 1572 5500
ApaLI G'TGCA_C
Cuts at: 5129 6285 5129
Size: 1156 5916
ApoI r'AATT_y
Cuts at: 1493 2297 2334 2413 2920 3359 3820 4238 4363
Size: 804 37 79 507 439 461 418 125
Cuts at: 4363 4381 4607 4759 6868 1493
Size: 18 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 507 461 439 418 226
152 125 79 37 18
AseI AT'TA_AT
Cuts at: 160 1032 5626 160
Size: 872 4594 1606
Fragments arranged by size:
4594 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
AvaI C'yCGr_G
Cuts at: 1870 1943 4397 1870
Size: 73 2454 4545
Fragments arranged by size:
4545 2454 73
BamHI G'GATC_C
Cuts at: 4402 4769 4402
Size: 367 6705
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4393 5848 618
Size: 325 217 627 2606 1455 1842
Fragments arranged by size:
2606 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4391 1391
Size: 3000 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2831 3320 3424 4020 928
Size: 971 932 489 104 596 3980
Fragments arranged by size:
3980 971 932 596 489 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5263 5297 1903
Size: 34 3326 34 3678
Fragments arranged by size:
3678 3326 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4964 6174 4964
Size: 1210 5862
BclI T'GATC_A
Cuts at: 1548 4228 4348 1548
Size: 2680 120 4272
Fragments arranged by size:
4272 2680 120
BfrBI ATG'CAT
Cuts at: 3923 3923
Size: 7072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5683 136
Size: 107 122 71 631 605 4011 1525
Fragments arranged by size:
4011 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3313 7067 3313
Size: 3754 3318
BlpI GC'TnA_GC
Cuts at: 4489 4489
Size: 7072
Bme1580I G_kGCm'C
Cuts at: 1790 4277 5133 6289 1790
Size: 2487 856 1156 2573
Fragments arranged by size:
2573 2487 1156 856
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7072
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4803 5761 442
Size: 416 53 83 399 24 3386 958 1753
Fragments arranged by size:
3386 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7072
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2199 2199
Size: 7072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 5732 1239
Size: 1122 3371 2579
Fragments arranged by size:
3371 2579 1122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 2726 2765 3019 5197 6062 6360 2474
Size: 252 39 254 2178 865 298 3186
Fragments arranged by size:
3186 2178 865 298 254 252 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5735 882
Size: 617 4236 2219
Fragments arranged by size:
4236 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4139 4863 493
Size: 711 2935 724 2702
Fragments arranged by size:
2935 2702 724 711
BsaBI GATnn'nnATC
Cuts at: 7066 7066
Size: 7072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4879 5261 5896 275
Size: 53 83 186 936 3346 382 635 1451
Fragments arranged by size:
3346 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5505 5903 6177 6324 6735 5505
Size: 398 274 147 411 5842
Fragments arranged by size:
5842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2104 2134 1178
Size: 30 165 30 701 30 6116
Fragments arranged by size:
6116 701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7072
BseYI C'CCAG_C
Cuts at: 3490 6275 6771 3490
Size: 2785 496 3791
Fragments arranged by size:
3791 2785 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3168 4303 4355 1163
Size: 573 1432 1135 52 3880
Fragments arranged by size:
3880 1432 1135 573 52
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4522 5283 6311 1540
Size: 275 186 2521 761 1028 2301
Fragments arranged by size:
2521 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5133 5218 6289 5133
Size: 85 1071 5916
Fragments arranged by size:
5916 1071 85
BsiWI C'GTAC_G
Cuts at: 3151 3151
Size: 7072
BsmI GAATG_Cn'
Cuts at: 2261 3374 4583 4676 2261
Size: 1113 1209 93 4657
Fragments arranged by size:
4657 1209 1113 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4277 4391 5133 5218 6289 1391
Size: 399 2487 114 742 85 1071 2174
Fragments arranged by size:
2487 2174 1071 742 399 114 85
BspEI T'CCGG_A
Cuts at: 6735 6735
Size: 7072
BspHI T'CATG_A
Cuts at: 4961 6691 4961
Size: 1730 5342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3183 4306 4427 844
Size: 2339 1123 121 3489
Fragments arranged by size:
3489 2339 1123 121
BsrBI CCG'CTC
Cuts at: 1394 1823 4959 1394
Size: 429 3136 3507
Fragments arranged by size:
3507 3136 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2027 5567 5741 65
Size: 1183 779 3540 174 1396
Fragments arranged by size:
3540 1396 1183 779 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5716 5903 1700
Size: 110 3906 187 2869
Fragments arranged by size:
3906 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7072
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7072
BssSI C'ACGA_G
Cuts at: 2184 5132 6144 2184
Size: 2948 1012 3112
Fragments arranged by size:
3112 2948 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3953 794
Size: 3159 3913
BstBI TT'CG_AA
Cuts at: 5876 5876
Size: 7072
BstEII G'GTnAC_C
Cuts at: 1184 2431 1184
Size: 1247 5825
BstXI CCAn_nnnn'nTGG
Cuts at: 2148 2148
Size: 7072
BstYI r'GATC_y
Cuts at: 1972 3313 4402 4769 5154 5171 6623 6709 6721
Size: 1341 1089 367 385 17 1452 86 12
Cuts at: 6721 7067 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1341 1089 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6333
Fragments arranged by size:
6333 683 56
BtsI GCAGTG_nn'
Cuts at: 3234 4588 5382 5402 3234
Size: 1354 794 20 4904
Fragments arranged by size:
4904 1354 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7037
DraI TTT'AAA
Cuts at: 2769 2916 3060 4376 2769
Size: 147 144 1316 5465
Fragments arranged by size:
5465 1316 147 144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4022 6079 809
Size: 507 2706 2057 1802
Fragments arranged by size:
2706 2057 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 4519 5408
Size: 54 1425 50 275 18 240 2449 889
Cuts at: 5408 7037 8
Size: 1629 43
Fragments arranged by size:
2449 1629 1425 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4519 1537
Size: 275 2707 4090
Fragments arranged by size:
4090 2707 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2652 2663 2958 3295 5002 1353
Size: 1026 273 11 295 337 1707 3423
Fragments arranged by size:
3423 1707 1026 337 295 273 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5659 6033 6179 387
Size: 1064 138 4070 374 146 1280
Fragments arranged by size:
4070 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2646 3314 3459 3768 5135 5732
Size: 393 729 285 668 145 309 1367 597
Cuts at: 5732 6519 6787 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1367 787 729 668 597 393 309
285 268 145
EcoRI G'AATT_C
Cuts at: 3820 4381 4759 3820
Size: 561 378 6133
Fragments arranged by size:
6133 561 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2370 2408 2465 2370
Size: 38 57 6977
Fragments arranged by size:
6977 57 38
FspI TGC'GCA
Cuts at: 5578 5578
Size: 7072
HaeII r_GCGC'y
Cuts at: 1678 6219 1678
Size: 4541 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 2672 2704 2705
Size: 32 135 32 657 32 411 32 1
Cuts at: 2705 2737 5713 5745 5787 5819 1373
Size: 32 2976 32 42 32 2626
Fragments arranged by size:
2976 2626 657 411 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3148 4130 4416 4662 669
Size: 988 281 1210 982 286 246 3079
Fragments arranged by size:
3079 1210 988 982 286 281 246
HindIII A'AGCT_T
Cuts at: 748 2638 2891 748
Size: 1890 253 4929
Fragments arranged by size:
4929 1890 253
HpaI GTT'AAC
Cuts at: 4662 4662
Size: 7072
KpnI G_GTAC'C
Cuts at: 4397 4397
Size: 7072
MfeI C'AATT_G
Cuts at: 2248 2739 4671 2248
Size: 491 1932 4649
Fragments arranged by size:
4649 1932 491
MluI A'CGCG_T
Cuts at: 6948 6948
Size: 7072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 2149 6186 6370 1305
Size: 759 85 4037 184 2007
Fragments arranged by size:
4037 2007 759 184 85
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 5010
Fragments arranged by size:
5010 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2426 4137 5030 5389 5548 518
Size: 648 1162 98 1711 893 359 159 2042
Fragments arranged by size:
2042 1711 1162 893 648 359 159 98
MspA1I CmG'CkG
Cuts at: 1606 4488 5165 6313 6558 1606
Size: 2882 677 1148 245 2120
Fragments arranged by size:
2882 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6520
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7072
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7072
NotI GC'GGCC_GC
Cuts at: 4519 4519
Size: 7072
NsiI A_TGCA'T
Cuts at: 3925 3925
Size: 7072
NspI r_CATG'y
Cuts at: 1132 1229 2425 3852 4253 4334 4430 5975 1132
Size: 97 1196 1427 401 81 96 1545 2229
Fragments arranged by size:
2229 1545 1427 1196 401 97 96 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7072
PciI A'CATG_T
Cuts at: 2421 4249 5971 2421
Size: 1828 1722 3522
Fragments arranged by size:
3522 1828 1722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7072
PmeI GTTT'AAAC
Cuts at: 4376 4376
Size: 7072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 6654 6686 5141
Size: 32 1481 32 5527
Fragments arranged by size:
5527 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7072
PsiI TTA'TAA
Cuts at: 2853 3351 3765 4642 2853
Size: 498 414 877 5283
Fragments arranged by size:
5283 877 498 414
PstI C_TGCA'G
Cuts at: 830 2982 3343 3538 4031 4424 830
Size: 2152 361 195 493 393 3478
Fragments arranged by size:
3478 2152 493 393 361 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7072
SalI G'TCGA_C
Cuts at: 1936 4414 1936
Size: 2478 4594
SapI GCTCTTCn'nnn_
Cuts at: 2379 2379
Size: 7072
SbfI CC_TGCA'GG
Cuts at: 4424 4424
Size: 7072
ScaI AGT'ACT
Cuts at: 2179 5320 7032 2179
Size: 3141 1712 2219
Fragments arranged by size:
3141 2219 1712
SfcI C'TryA_G
Cuts at: 826 1045 2978 3339 3510 3534 4027 4420 5555
Size: 219 1933 361 171 24 493 393 1135
Cuts at: 5555 6236 6427 826
Size: 681 191 1471
Fragments arranged by size:
1933 1471 1135 681 493 393 361 219
191 171 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7072
SmaI CCC'GGG
Cuts at: 1872 4399 1872
Size: 2527 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2705 2780 3034 5176 6077
Size: 197 926 510 252 75 254 2142 901
Cuts at: 6077 6339 820
Size: 262 1553
Fragments arranged by size:
2142 1553 926 901 510 262 254 252
197 75
SnaBI TAC'GTA
Cuts at: 493 4863 493
Size: 4370 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7072
SphI G_CATG'C
Cuts at: 1132 4430 1132
Size: 3298 3774
SspI AAT'ATT
Cuts at: 5 52 4996 5
Size: 47 4944 2081
Fragments arranged by size:
4944 2081 47
StuI AGG'CCT
Cuts at: 1517 2448 1517
Size: 931 6141
StyI C'CwwG_G
Cuts at: 513 1065 2266 4511 513
Size: 552 1201 2245 3074
Fragments arranged by size:
3074 2245 1201 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5099 5116 5269 5454 5899 325
Size: 1255 3519 17 153 185 445 1498
Fragments arranged by size:
3519 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2177 2254 2348
Size: 274 80 33 51 1443 200 77 94
Cuts at: 2348 3751 5318 7030 96
Size: 1403 1567 1712 138
Fragments arranged by size:
1712 1567 1443 1403 274 200 138 94
80 77 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 7032 7064 2104
Size: 32 4896 32 2112
Fragments arranged by size:
4896 2112 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7072
XbaI T'CTAG_A
Cuts at: 3784 4408 3784
Size: 624 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7072
XmaI C'CCGG_G
Cuts at: 1870 4397 1870
Size: 2527 4545
XmnI GAAnn'nnTTC
Cuts at: 2672 3090 5201 2672
Size: 418 2111 4543
Fragments arranged by size:
4543 2111 418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4880 276
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI