Restriction map for
pFN21AB8591
(Circular) (Six-base) MAPSORT of: pFN21AB8591.seq Check: 7366 from: 1 to: 7015
pFN21AB8591 7015 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4825 278
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4358 1937
Size: 2421 4594
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3061 4188 5078 6462 6730 1632
Size: 1429 1127 890 1384 268 1917
Fragments arranged by size:
1917 1429 1384 1127 890 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 4295 5914 6891 1203
Size: 3092 1619 977 1327
Fragments arranged by size:
3092 1619 1327 977
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 3113 3664 5744 3113
Size: 551 2080 4384
Fragments arranged by size:
4384 2080 551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2955 1184
Size: 1771 5244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2122 2154 2122
Size: 32 6983
AlwNI CAG_nnn'CTG
Cuts at: 2543 2903 4758 6330 2543
Size: 360 1855 1572 3228
Fragments arranged by size:
3228 1855 1572 360
ApaI G_GGCC'C
Cuts at: 3973 3973
Size: 7015
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 4324 4550 4702 6811 1493
Size: 2831 226 152 2109 1697
Fragments arranged by size:
2831 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2039 2247 2769 2834 4340 1870
Size: 73 89 7 208 522 65 1506 4545
Fragments arranged by size:
4545 1506 522 208 89 73 65 7
BamHI G'GATC_C
Cuts at: 2215 4345 4712 2215
Size: 2130 367 4518
Fragments arranged by size:
4518 2130 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2243 2426 3798 4336
Size: 325 217 627 335 121 183 1372 538
Cuts at: 4336 5791 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1372 627 538 335 325 217
183 121
BanII G_rGCy'C
Cuts at: 1391 2708 3415 3973 4117 4285 4334 1391
Size: 1317 707 558 144 168 49 4072
Fragments arranged by size:
4072 1317 707 558 168 144 49
BbeI G_GCGC'C
Cuts at: 2247 2247
Size: 7015
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2852 928
Size: 971 953 5091
Fragments arranged by size:
5091 971 953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5206 5240 1903
Size: 34 50 34 3185 34 3678
Fragments arranged by size:
3678 3185 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2110 4907 6117 2110
Size: 2797 1210 3008
Fragments arranged by size:
3008 2797 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2665 5626
Size: 107 122 71 631 605 337 656 2961
Cuts at: 5626 136
Size: 1525
Fragments arranged by size:
2961 1525 656 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3433 7010 3433
Size: 3577 3438
BlpI GC'TnA_GC
Cuts at: 2087 2529 3414 3419 3775 4432 2087
Size: 442 885 5 356 657 4670
Fragments arranged by size:
4670 885 657 442 356 5
Bme1580I G_kGCm'C
Cuts at: 1790 2127 3135 3973 5076 6232 1790
Size: 337 1008 838 1103 1156 2573
Fragments arranged by size:
2573 1156 1103 1008 838 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3581 3801
Size: 416 53 83 399 24 1578 586 220
Cuts at: 3801 4287 4746 5704 442
Size: 486 459 958 1753
Fragments arranged by size:
1753 1578 958 586 486 459 416 399
220 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 3434 2173
Size: 1261 5754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2837 2905 3168 3536 3619 3723 3866 5675
Size: 1598 68 263 368 83 104 143 1809
Cuts at: 5675 1239
Size: 2579
Fragments arranged by size:
2579 1809 1598 368 263 143 104 83
68
Bpu10I CC'TnA_GC
Cuts at: 2904 2955 2904
Size: 51 6964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2722 5140 6005 6303 2513
Size: 209 2418 865 298 3225
Fragments arranged by size:
3225 2418 865 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2284 5678 882
Size: 617 785 3394 2219
Fragments arranged by size:
3394 2219 785 617
BsaAI yAC'GTr
Cuts at: 493 1204 4806 493
Size: 711 3602 2702
Fragments arranged by size:
3602 2702 711
BsaBI GATnn'nnATC
Cuts at: 7009 7009
Size: 7015
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2244 4051 4822 5204
Size: 53 83 186 936 711 1807 771 382
Cuts at: 5204 5839 275
Size: 635 1451
Fragments arranged by size:
1807 1451 936 771 711 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5448 5846 6120 6267 6678 5448
Size: 398 274 147 411 5785
Fragments arranged by size:
5785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4139 4169 4267 4297 1178
Size: 30 165 30 2736 30 98 30 3896
Fragments arranged by size:
3896 2736 165 98 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 3670 4275 1186
Size: 1219 1265 605 3926
Fragments arranged by size:
3926 1265 1219 605
BseYI C'CCAG_C
Cuts at: 2552 2677 2707 3135 4258 6218 6714 2552
Size: 125 30 428 1123 1960 496 2853
Fragments arranged by size:
2853 1960 1123 496 428 125 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3804 1163
Size: 573 2068 4374
Fragments arranged by size:
4374 2068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 4465 5226 6254 1540
Size: 275 186 106 2358 761 1028 2301
Fragments arranged by size:
2358 2301 1028 761 275 186 106
BsiHKAI G_wGCw'C
Cuts at: 3363 3415 3642 5076 5161 6232 3363
Size: 52 227 1434 85 1071 4146
Fragments arranged by size:
4146 1434 1071 227 85 52
BsmI GAATG_Cn'
Cuts at: 4526 4619 4526
Size: 93 6922
BsmBI CGTCTCn'nnnn_
Cuts at: 2727 3554 2727
Size: 827 6188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2708 3135 3363 3415 3642 3973
Size: 399 337 581 427 228 52 227 331
Cuts at: 3973 4117 4285 4334 5076 5161 6232 1391
Size: 144 168 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 427 399 337 331
228 227 168 144 85 52 49
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 4904 6634 4904
Size: 1730 5285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 2895 4370 844
Size: 1626 425 1475 3489
Fragments arranged by size:
3489 1626 1475 425
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 2118 4177 4902 1394
Size: 429 268 27 2059 725 3507
Fragments arranged by size:
3507 2059 725 429 268 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 2394 5510 5684 65
Size: 1183 1146 3116 174 1396
Fragments arranged by size:
3116 1396 1183 1146 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 5659 5846 1700
Size: 110 198 3651 187 2869
Fragments arranged by size:
3651 2869 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 5075 6087 5075
Size: 1012 6003
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7015
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 3328 3856 1184
Size: 2144 528 4343
Fragments arranged by size:
4343 2144 528
BstXI CCAn_nnnn'nTGG
Cuts at: 2620 2715 3809 2620
Size: 95 1094 5826
Fragments arranged by size:
5826 1094 95
BstYI r'GATC_y
Cuts at: 1972 2215 3433 4345 4712 5097 5114 6566 6652
Size: 243 1218 912 367 385 17 1452 86
Cuts at: 6652 6664 7010 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1218 912 385 367 346 243
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2319 2319
Size: 7015
BtgI C'CryG_G
Cuts at: 513 1065 2694 2804 3732 3870 513
Size: 552 1629 110 928 138 3658
Fragments arranged by size:
3658 1629 928 552 138 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6276
Fragments arranged by size:
6276 683 56
BtsI GCAGTG_nn'
Cuts at: 2625 3405 4213 4531 5325 5345 2625
Size: 780 808 318 794 20 4295
Fragments arranged by size:
4295 808 794 780 318 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DraIII CAC_nnn'GTG
Cuts at: 3328 3979 3328
Size: 651 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6022 809
Size: 507 4706 1802
Fragments arranged by size:
4706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2500 2911
Size: 54 1425 50 275 18 274 396 411
Cuts at: 2911 3271 3544 4194 4462 5351 6980 8
Size: 360 273 650 268 889 1629 43
Fragments arranged by size:
1629 1425 889 650 411 396 360 275
274 273 268 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2104 4462 1537
Size: 275 292 2358 4090
Fragments arranged by size:
4090 2358 292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4945 1353
Size: 3592 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5602 5976 6122 387
Size: 1064 138 4013 374 146 1280
Fragments arranged by size:
4013 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3413 3413
Size: 7015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2837 2905 3061 3168 3536 3619 3723
Size: 393 1205 68 156 107 368 83 104
Cuts at: 3723 3866 4188 5078 5675 6462 6730 1239
Size: 143 322 890 597 787 268 1524
Fragments arranged by size:
1524 1205 890 787 597 393 368 322
268 156 143 107 104 83 68
EcoNI CCTnn'n_nnAGG
Cuts at: 3578 3578
Size: 7015
EcoO109I rG'GnC_Cy
Cuts at: 2036 2560 2830 2900 3583 2036
Size: 524 270 70 683 5468
Fragments arranged by size:
5468 683 524 270 70
EcoRI G'AATT_C
Cuts at: 4324 4702 4324
Size: 378 6637
FspI TGC'GCA
Cuts at: 3747 5521 3747
Size: 1774 5241
HaeII r_GCGC'y
Cuts at: 1678 2247 3879 6162 1678
Size: 569 1632 2283 2531
Fragments arranged by size:
2531 2283 1632 569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 3099 3131 3141
Size: 32 135 32 635 32 860 32 10
Cuts at: 3141 3173 3397 3429 3636 3650 3665 3668 3682
Size: 32 224 32 207 14 15 3 14
Cuts at: 3682 3697 4139 4171 4292 4324 5656 5688 5730
Size: 15 442 32 121 32 1332 32 42
Cuts at: 5730 5762 1373
Size: 32 2626
Fragments arranged by size:
2626 1332 860 635 442 224 207 135
121 42 32 32 32 32 32 32
32 32 32 32 15 15 14 14
10 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4359 4605 669
Size: 988 281 2421 246 3079
Fragments arranged by size:
3079 2421 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7015
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KasI G'GCGC_C
Cuts at: 2243 2243
Size: 7015
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3064 6129 6313 1305
Size: 1759 3065 184 2007
Fragments arranged by size:
3065 2007 1759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3273 4196 10
Size: 54 1425 343 1441 923 2829
Fragments arranged by size:
2829 1441 1425 923 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4973 5332 5491 518
Size: 648 3807 359 159 2042
Fragments arranged by size:
3807 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2533 2543 3266 3489 3743 4186 4431 5108
Size: 927 10 723 223 254 443 245 677
Cuts at: 5108 6256 6501 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 927 723 677 443 254 245
245 223 10
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7015
NarI GG'CG_CC
Cuts at: 2244 2244
Size: 7015
NcoI C'CATG_G
Cuts at: 513 1065 3870 513
Size: 552 2805 3658
Fragments arranged by size:
3658 2805 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 3752 4299 4373 5918 1132
Size: 97 2523 547 74 1545 2229
Fragments arranged by size:
2523 2229 1545 547 97 74
PasI CC'CwG_GG
Cuts at: 1348 3863 1348
Size: 2515 4500
PciI A'CATG_T
Cuts at: 4295 5914 4295
Size: 1619 5396
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3269 3979 4124 1108
Size: 2161 710 145 3999
Fragments arranged by size:
3999 2161 710 145
PfoI T'CCnGG_A
Cuts at: 3228 3228
Size: 7015
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 6597 6629 5084
Size: 32 1481 32 5470
Fragments arranged by size:
5470 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2560 2830 3583 2036
Size: 524 270 753 5468
Fragments arranged by size:
5468 753 524 270
PshAI GACnn'nnGTC
Cuts at: 1613 2160 2367 1613
Size: 547 207 6261
Fragments arranged by size:
6261 547 207
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PspOMI G'GGCC_C
Cuts at: 3969 3969
Size: 7015
PstI C_TGCA'G
Cuts at: 830 2934 4367 830
Size: 2104 1433 3478
Fragments arranged by size:
3478 2104 1433
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 2543 3266 3489 3743 2543
Size: 723 223 254 5815
Fragments arranged by size:
5815 723 254 223
SacI G_AGCT'C
Cuts at: 3415 3415
Size: 7015
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SanDI GG'GwC_CC
Cuts at: 2036 2036
Size: 7015
SbfI CC_TGCA'GG
Cuts at: 2934 4367 2934
Size: 1433 5582
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 2049 2338 2930 3291 3738 4138 4363
Size: 219 1004 289 592 361 447 400 225
Cuts at: 4363 5498 6179 6370 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1004 681 592 447 400 361
289 225 219 191
SfoI GGC'GCC
Cuts at: 2245 2245
Size: 7015
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 2034 2041 2249 4342 1872
Size: 162 7 208 2093 4545
Fragments arranged by size:
4545 2093 208 162 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 2701 5119 6020 6282 820
Size: 197 926 549 209 2418 901 262 1553
Fragments arranged by size:
2418 1553 926 901 549 262 209 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7015
StyI C'CwwG_G
Cuts at: 513 1065 3870 4454 513
Size: 552 2805 584 3074
Fragments arranged by size:
3074 2805 584 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3252 5042 5059 5212 5397 5842 325
Size: 1255 1672 1790 17 153 185 445 1498
Fragments arranged by size:
1790 1672 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5261 6973 96
Size: 274 80 33 51 1443 3284 1712 138
Fragments arranged by size:
3284 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3834 3866 4218 4250 6975 7007 3834
Size: 32 352 32 2725 32 3842
Fragments arranged by size:
3842 2725 352 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3587 1316
Size: 2271 4744
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3143 3594 3777 3143
Size: 451 183 6381
Fragments arranged by size:
6381 451 183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 2032 2039 2247 4340 1870
Size: 162 7 208 2093 4545
Fragments arranged by size:
4545 2093 208 162 7
XmnI GAAnn'nnTTC
Cuts at: 5144 5144
Size: 7015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4823 276
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SrfI SwaI