Restriction map for
pFN21AB8580
(Circular) (Six-base) MAPSORT of: pFN21AB8580.seq Check: 4008 from: 1 to: 6151
pFN21AB8580 6151 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3358 3358
Size: 6151
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2719 3494 1937
Size: 782 775 4594
Fragments arranged by size:
4594 782 775
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 4214 5598 5866 1632
Size: 557 2025 1384 268 1917
Fragments arranged by size:
2025 1917 1384 557 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 6151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 2870 3894 5466 2870
Size: 1024 1572 3555
Fragments arranged by size:
3555 1572 1024
ApaI G_GGCC'C
Cuts at: 2069 2112 2264 2069
Size: 43 152 5956
Fragments arranged by size:
5956 152 43
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2510 2855 2883 3426 3460 3686 3838 5947
Size: 1017 345 28 543 34 226 152 2109
Cuts at: 5947 1493
Size: 1697
Fragments arranged by size:
2109 1697 1017 543 345 226 152 34
28
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2943 3476 1870
Size: 73 72 928 533 4545
Fragments arranged by size:
4545 928 533 73 72
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 3472 4927 618
Size: 325 217 627 456 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 456 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2112 2264 2382 3470 1391
Size: 678 43 152 118 1088 4072
Fragments arranged by size:
4072 1088 678 152 118 43
BbeI G_GCGC'C
Cuts at: 2247 2247
Size: 6151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BbvCI CC'TCA_GC
Cuts at: 2052 2583 2052
Size: 531 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2952 2986 4342 4376 1903
Size: 34 1015 34 1356 34 3678
Fragments arranged by size:
3678 1356 1015 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 4762 136
Size: 107 122 71 631 605 721 2369 1525
Fragments arranged by size:
2369 1525 721 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 2207 3568 2207
Size: 1361 4790
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2112 2264 4212 5368 1790
Size: 279 43 152 1948 1156 2573
Fragments arranged by size:
2573 1948 1156 279 152 43
BmgBI CAC'GTC
Cuts at: 1318 2349 2374 1318
Size: 1031 25 5095
Fragments arranged by size:
5095 1031 25
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2237 3882 4840
Size: 416 53 83 399 24 820 1645 958
Cuts at: 4840 442
Size: 1753
Fragments arranged by size:
1753 1645 958 820 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2717
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4811 1239
Size: 3572 2579
Bpu10I CC'TnA_GC
Cuts at: 2052 2583 2052
Size: 531 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2162 2244 2319
Size: 53 83 186 936 509 120 82 75
Cuts at: 2319 3958 4340 4975 275
Size: 1639 382 635 1451
Fragments arranged by size:
1639 1451 936 635 509 382 186 120
83 82 75 53
BsaWI w'CCGG_w
Cuts at: 2829 4584 4982 5256 5403 5814 2829
Size: 1755 398 274 147 411 3166
Fragments arranged by size:
3166 1755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2721 2751 1178
Size: 30 165 30 624 30 664 30 4578
Fragments arranged by size:
4578 664 624 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6151
BseYI C'CCAG_C
Cuts at: 2030 2255 2485 2742 3027 5354 5850 2030
Size: 225 230 257 285 2327 496 2331
Fragments arranged by size:
2331 2327 496 285 257 230 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2661 2700 1163
Size: 573 925 39 4614
Fragments arranged by size:
4614 925 573 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2301 2355 3601 4362 5390 1540
Size: 275 186 300 54 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 300 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 2996 3662 3755 2996
Size: 666 93 5392
Fragments arranged by size:
5392 666 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2112 2264 2382 3470 4212 4297
Size: 399 279 43 152 118 1088 742 85
Cuts at: 4297 5368 1391
Size: 1071 2174
Fragments arranged by size:
2174 1088 1071 742 399 279 152 118
85 43
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 3358 3506 844
Size: 2011 503 148 3489
Fragments arranged by size:
3489 2011 503 148
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4646 4820 65
Size: 1183 3398 174 1396
Fragments arranged by size:
3398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2300 3191 4795 4982 1700
Size: 110 490 891 1604 187 2869
Fragments arranged by size:
2869 1604 891 490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2540 2639 2870 794
Size: 1746 99 231 4075
Fragments arranged by size:
4075 1746 231 99
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3078 3107 3252 3481 3848 4233 4250 5702
Size: 1106 29 145 229 367 385 17 1452
Cuts at: 5702 5788 5800 6146 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1106 385 367 346 229 145
86 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 4594
Fragments arranged by size:
4594 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2226 487
Size: 683 56 1000 4412
Fragments arranged by size:
4412 1000 683 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DraIII CAC_nnn'GTG
Cuts at: 2504 2678 2504
Size: 174 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2161 5158 809
Size: 507 845 2997 1802
Fragments arranged by size:
2997 1802 845 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2340 2352
Size: 54 1425 50 275 18 468 42 12
Cuts at: 2352 3598 4487 6116 8
Size: 1246 889 1629 43
Fragments arranged by size:
1629 1425 1246 889 468 275 54 50
43 42 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2298 2352 3598 1537
Size: 275 486 54 1246 4090
Fragments arranged by size:
4090 1246 486 275 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 4081 1353
Size: 842 1886 3423
Fragments arranged by size:
3423 1886 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 4738 5112 5258 387
Size: 1064 138 483 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 483 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 4214 4811 5598 5866 1239
Size: 393 557 2025 597 787 268 1524
Fragments arranged by size:
2025 1524 787 597 557 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2066 2108 2109 2260 2867 2066
Size: 42 1 151 607 5350
Fragments arranged by size:
5350 607 151 42 1
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 6151
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2179 2247 2310 2399 5298 1678
Size: 501 68 63 89 2899 2531
Fragments arranged by size:
2899 2531 501 89 68 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2961 2993 3034
Size: 32 135 32 455 32 902 32 41
Cuts at: 3034 3066 4792 4824 4866 4898 1373
Size: 32 1726 32 42 32 2626
Fragments arranged by size:
2626 1726 902 455 135 42 41 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2720 2932 3495 3741 669
Size: 988 281 782 212 563 246 3079
Fragments arranged by size:
3079 988 782 563 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KasI G'GCGC_C
Cuts at: 2243 2243
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 2847 3750 2847
Size: 903 5248
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3070 5265 5449 1305
Size: 1765 2195 184 2007
Fragments arranged by size:
2195 2007 1765 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 3819
Fragments arranged by size:
3819 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4109 4468 4627 518
Size: 648 2943 359 159 2042
Fragments arranged by size:
2943 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2072 2128 2255 3027 3567 4244 5392
Size: 424 42 56 127 772 540 677 1148
Cuts at: 5392 5637 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 772 677 540 424 245 127
56 42
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 6151
NarI GG'CG_CC
Cuts at: 2244 2244
Size: 6151
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 3282 3509 5054 1132
Size: 97 2053 227 1545 2229
Fragments arranged by size:
2229 2053 1545 227 97
PasI CC'CwG_GG
Cuts at: 1348 3175 1348
Size: 1827 4324
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2867 2867
Size: 6151
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PspOMI G'GGCC_C
Cuts at: 2065 2108 2260 2065
Size: 43 152 5956
Fragments arranged by size:
5956 152 43
PstI C_TGCA'G
Cuts at: 830 2127 3503 830
Size: 1297 1376 3478
Fragments arranged by size:
3478 1376 1297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2030 2030
Size: 6151
SacII CC_GC'GG
Cuts at: 2073 2073
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 2718 3493 1936
Size: 782 775 4594
Fragments arranged by size:
4594 782 775
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2123 3084 3123 3499 4634 5315 5506
Size: 219 1078 961 39 376 1135 681 191
Cuts at: 5506 826
Size: 1471
Fragments arranged by size:
1471 1135 1078 961 681 376 219 191
39
SfoI GGC'GCC
Cuts at: 2245 2245
Size: 6151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4255 5156 5418 820
Size: 197 926 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2490 3590 513
Size: 552 939 153 333 1100 3074
Fragments arranged by size:
3074 1100 939 552 333 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2976 4178 4195 4348 4533 4978 325
Size: 1255 1396 1202 17 153 185 445 1498
Fragments arranged by size:
1498 1396 1255 1202 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 2602 3487 2602
Size: 885 5266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI