Restriction map for
pFN21AB8579
(Circular) (Six-base) MAPSORT of: pFN21AB8579.seq Check: 8845 from: 1 to: 5401
pFN21AB8579 5401 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2451 3211 278
Size: 53 83 186 936 915 760 2468
Fragments arranged by size:
2468 936 915 760 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2599 3464 4848 5116 1632
Size: 967 865 1384 268 1917
Fragments arranged by size:
1917 1384 967 865 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 2167 4130 2167
Size: 1963 3438
AleI CACnn'nnGTG
Cuts at: 1166 2280 1166
Size: 1114 4287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AlwNI CAG_nnn'CTG
Cuts at: 2302 2578 2626 3144 4716 2302
Size: 276 48 518 1572 2987
Fragments arranged by size:
2987 1572 518 276 48
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2726 1870
Size: 73 175 608 4545
Fragments arranged by size:
4545 608 175 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 2722 4177 618
Size: 325 217 627 725 210 1455 1842
Fragments arranged by size:
1842 1455 725 627 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2159 2336 2720 1391
Size: 768 177 384 4072
Fragments arranged by size:
4072 768 384 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 928
Size: 971 315 4115
Fragments arranged by size:
4115 971 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3592 3626 1903
Size: 34 50 34 1571 34 3678
Fragments arranged by size:
3678 1571 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2385 4012 136
Size: 107 122 71 631 605 713 1627 1525
Fragments arranged by size:
1627 1525 713 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2098 2633 2818 2098
Size: 535 185 4681
Fragments arranged by size:
4681 535 185
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3462 4618 1790
Size: 727 945 1156 2573
Fragments arranged by size:
2573 1156 945 727
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 4090 442
Size: 416 53 83 399 24 1715 958 1753
Fragments arranged by size:
1753 1715 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 4061 1239
Size: 932 1890 2579
Fragments arranged by size:
2579 1890 932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3192 493
Size: 711 1988 2702
Fragments arranged by size:
2702 1988 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2341 2448 3208 3590
Size: 53 83 186 936 808 107 760 382
Cuts at: 3590 4225 275
Size: 635 1451
Fragments arranged by size:
1451 936 808 760 635 382 186 107
83 53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 4435
Fragments arranged by size:
4435 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2138 2708 1186
Size: 849 103 570 3879
Fragments arranged by size:
3879 849 570 103
BseYI C'CCAG_C
Cuts at: 2329 2504 4604 5100 2329
Size: 175 2100 496 2630
Fragments arranged by size:
2630 2100 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2090 2851 3612 4640 1540
Size: 275 186 89 761 761 1028 2301
Fragments arranged by size:
2301 1028 761 761 275 186 89
BsiHKAI G_wGCw'C
Cuts at: 2159 2639 3462 3547 4618 2159
Size: 480 823 85 1071 2942
Fragments arranged by size:
2942 1071 823 480 85
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2336 2517 2639 2720 3462 3547
Size: 399 369 177 181 122 81 742 85
Cuts at: 3547 4618 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 181 177 122
85 81
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 844
Size: 1912 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 3288 1394
Size: 429 316 1149 3507
Fragments arranged by size:
3507 1149 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4045 4232 1700
Size: 110 2235 187 2869
Fragments arranged by size:
2869 2235 187 110
BsrGI T'GTAC_A
Cuts at: 96 2667 96
Size: 2571 2830
BssHII G'CGCG_C
Cuts at: 1925 2348 1925
Size: 423 4978
BssSI C'ACGA_G
Cuts at: 2143 2534 3461 4473 2143
Size: 391 927 1012 3071
Fragments arranged by size:
3071 1012 927 391
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 2472 794
Size: 1508 170 3723
Fragments arranged by size:
3723 1508 170
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2149
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2568 2731 3098 3483 3500 4952 5038 5050
Size: 596 163 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 596 385 367 346 163 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2265 2265
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4849
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DraIII CAC_nnn'GTG
Cuts at: 2482 2482
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2848 3737
Size: 54 1425 50 275 18 257 761 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 761 275 257 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2087 2848 1537
Size: 275 275 761 4090
Fragments arranged by size:
4090 761 275 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 3331 1353
Size: 700 1278 3423
Fragments arranged by size:
3423 1278 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2578 3988 4362 4508 387
Size: 1064 138 989 1410 374 146 1280
Fragments arranged by size:
1410 1280 1064 989 374 146 138
EcoICRI GAG'CTC
Cuts at: 2157 2157
Size: 5401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2599 3464 4061 4848 5116 1239
Size: 393 539 428 865 597 787 268 1524
Fragments arranged by size:
1524 865 787 597 539 428 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 2318
Size: 5401
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2441 4548 1678
Size: 763 2107 2531
Fragments arranged by size:
2531 2107 763
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2153 2185 2216
Size: 32 135 32 440 32 109 32 31
Cuts at: 2216 2248 4042 4074 4116 4148 1373
Size: 32 1794 32 42 32 2626
Fragments arranged by size:
2626 1794 440 135 109 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3579
Fragments arranged by size:
3579 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 3359 3718 3877 518
Size: 648 1114 1079 359 159 2042
Fragments arranged by size:
2042 1114 1079 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2491 2527 2683 2817 3494 4642 4887
Size: 693 192 36 156 134 677 1148 245
Cuts at: 4887 1606
Size: 2120
Fragments arranged by size:
2120 1148 693 677 245 192 156 134
36
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4849
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 1108
Size: 1374 4027
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2307 2753 830
Size: 1477 446 3478
Fragments arranged by size:
3478 1477 446
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2299 2491 2527 2683 2299
Size: 192 36 156 5017
Fragments arranged by size:
5017 192 156 36
SacI G_AGCT'C
Cuts at: 2159 2159
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2303 2410 2749 3884 4565 4756 826
Size: 219 1258 107 339 1135 681 191 1471
Fragments arranged by size:
1471 1258 1135 681 339 219 191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2120 2728 1872
Size: 248 608 4545
Fragments arranged by size:
4545 608 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3505 4406 4668 820
Size: 197 926 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2840 513
Size: 552 1775 3074
Fragments arranged by size:
3074 1775 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3428 3445 3598 3783 4228 325
Size: 1255 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 3647 5359
Size: 274 80 33 51 1443 690 980 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1443 980 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2118 2726 1870
Size: 248 608 4545
Fragments arranged by size:
4545 608 248
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2449 3209 276
Size: 53 83 186 936 915 760 2468
Fragments arranged by size:
2468 936 915 760 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI