Restriction map for
pFN21AB8575
(Circular) (Six-base) MAPSORT of: pFN21AB8575.seq Check: 2332 from: 1 to: 5722
pFN21AB8575 5722 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3065 1937
Size: 475 653 4594
Fragments arranged by size:
4594 653 475
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2846 3785 5169 5437 1632
Size: 1214 939 1384 268 1917
Fragments arranged by size:
1917 1384 1214 939 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 2913 4621 5598 1203
Size: 1710 1708 977 1327
Fragments arranged by size:
1710 1708 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 2470 2783 3465 5037 2470
Size: 313 682 1572 3155
Fragments arranged by size:
3155 1572 682 313
ApaLI G'TGCA_C
Cuts at: 2072 2393 3779 4935 2072
Size: 321 1386 1156 2859
Fragments arranged by size:
2859 1386 1156 321
ApoI r'AATT_y
Cuts at: 1493 3031 3257 3409 5518 1493
Size: 1538 226 152 2109 1697
Fragments arranged by size:
2109 1697 1538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2760 3047 1870
Size: 73 817 287 4545
Fragments arranged by size:
4545 817 287 73
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3043 4498 618
Size: 325 217 627 330 926 1455 1842
Fragments arranged by size:
1842 1455 926 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 3041 1391
Size: 1650 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 928
Size: 971 680 4071
Fragments arranged by size:
4071 971 680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 2428 1548
Size: 880 4842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2675 4333 136
Size: 107 122 71 631 605 1003 1658 1525
Fragments arranged by size:
1658 1525 1003 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 2972 3139 2972
Size: 167 5555
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2122 2397 3783 4939 1790
Size: 286 46 275 1386 1156 2573
Fragments arranged by size:
2573 1386 1156 286 275 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2963 1056
Size: 1907 3815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 4382 1239
Size: 947 2196 2579
Fragments arranged by size:
2579 2196 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 2442 5716 2442
Size: 3274 2448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 2459 2489 1178
Size: 30 165 30 607 30 419 30 4411
Fragments arranged by size:
4411 607 419 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5722
BseYI C'CCAG_C
Cuts at: 4925 5421 4925
Size: 496 5226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 1163
Size: 573 474 4675
Fragments arranged by size:
4675 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2397 3783 3868 4939 2076
Size: 321 1386 85 1071 2859
Fragments arranged by size:
2859 1386 1071 321 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
BsmBI CGTCTCn'nnnn_
Cuts at: 2996 2996
Size: 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2122 2397 3041 3783 3868 4939
Size: 399 286 46 275 644 742 85 1071
Cuts at: 4939 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 644 399 286 275 85
46
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 3609 1394
Size: 429 812 974 3507
Fragments arranged by size:
3507 974 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 2868 4217 4391 65
Size: 1183 1338 282 1349 174 1396
Fragments arranged by size:
1396 1349 1338 1183 282 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2361 3052 3419 3804 3821 5273 5359 5371
Size: 389 691 367 385 17 1452 86 12
Cuts at: 5371 5717 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 691 389 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2478 513
Size: 552 939 474 3757
Fragments arranged by size:
3757 939 552 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2395 2793 2941 3238 4032 4052 2307
Size: 88 398 148 297 794 20 3977
Fragments arranged by size:
3977 794 398 297 148 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3006 3169 4058
Size: 54 1425 50 275 18 1176 163 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1176 889 275 163 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2079 3652 1353
Size: 713 13 1573 3423
Fragments arranged by size:
3423 1573 713 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2846 3785 4382 5169 5437 1239
Size: 393 554 660 939 597 787 268 1524
Fragments arranged by size:
1524 939 787 660 597 554 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2344 2467 2344
Size: 123 5599
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2992 2908
Size: 84 5638
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 2648 4869 1678
Size: 970 2221 2531
Fragments arranged by size:
2531 2221 970
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 4363 4395 4437
Size: 32 135 32 887 32 1872 32 42
Cuts at: 4437 4469 1373
Size: 32 2626
Fragments arranged by size:
2626 1872 887 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2579 2974 748
Size: 1831 395 3496
Fragments arranged by size:
3496 1831 395
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2901 4836 5020 1305
Size: 1596 1935 184 2007
Fragments arranged by size:
2007 1935 1596 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3900
Fragments arranged by size:
3900 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 2918 3680 4039 4198 518
Size: 648 1632 120 762 359 159 2042
Fragments arranged by size:
2042 1632 762 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2069 2139 2851 3138 3815 4963 5208 1606
Size: 463 70 712 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 712 677 463 287 245 70
NcoI C'CATG_G
Cuts at: 513 1065 2004 2478 513
Size: 552 939 474 3757
Fragments arranged by size:
3757 939 552 474
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 2959 1052
Size: 1907 3815
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2917 3080 4625 1132
Size: 97 1688 163 1545 2229
Fragments arranged by size:
2229 1688 1545 163 97
PasI CC'CwG_GG
Cuts at: 1348 2486 1348
Size: 1138 4584
PciI A'CATG_T
Cuts at: 2913 4621 2913
Size: 1708 4014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2467
Size: 5722
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2390 2850 3074 830
Size: 1560 460 224 3478
Fragments arranged by size:
3478 1560 460 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2069 2851 2069
Size: 782 4940
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2386 2846 3070 4205 4886 5077 826
Size: 219 1341 460 224 1135 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 460 224 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 2762 3049 1872
Size: 890 287 4545
Fragments arranged by size:
4545 890 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 2237 152
Size: 2085 3637
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 2997 3161 513
Size: 552 939 474 519 164 3074
Fragments arranged by size:
3074 939 552 519 474 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3968 5680
Size: 274 80 33 51 1443 882 1109 1712
Cuts at: 5680 96
Size: 138
Fragments arranged by size:
1712 1443 1109 882 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2010 2042 2227 2259 5682 5714 2010
Size: 32 185 32 3423 32 2018
Fragments arranged by size:
3423 2018 185 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 2760 3047 1870
Size: 890 287 4545
Fragments arranged by size:
4545 890 287
XmnI GAAnn'nnTTC
Cuts at: 2381 3851 2381
Size: 1470 4252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI