Restriction map for
pFN21AB8574
(Circular) (Six-base) MAPSORT of: pFN21AB8574.seq Check: 7024 from: 1 to: 7048
pFN21AB8574 7048 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2800 3799 4391 1937
Size: 863 999 592 4594
Fragments arranged by size:
4594 999 863 592
Acc65I G'GTAC_C
Cuts at: 4369 4369
Size: 7048
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 3401 3597 3857 4018 5111 6495 6763
Size: 1200 569 196 260 161 1093 1384 268
Cuts at: 6763 1632
Size: 1917
Fragments arranged by size:
1917 1384 1200 1093 569 268 260 196
161
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6851
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 3795 5777 3795
Size: 1982 5066
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3707 1184
Size: 2523 4525
AlwNI CAG_nnn'CTG
Cuts at: 2105 4013 4135 4791 6363 2105
Size: 1908 122 656 1572 2790
Fragments arranged by size:
2790 1908 1572 656 122
ApaI G_GGCC'C
Cuts at: 4051 4187 4051
Size: 136 6912
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 2435 3008 3062 4336 4342 4357 4583 4735
Size: 942 573 54 1274 6 15 226 152
Cuts at: 4735 6844 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1274 942 573 226 152 54
15 6
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 4373 1870
Size: 73 1560 870 4545
Fragments arranged by size:
4545 1560 870 73
BamHI G'GATC_C
Cuts at: 2827 4378 4745 2827
Size: 1551 367 5130
Fragments arranged by size:
5130 1551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 4369 5824 618
Size: 325 217 627 2372 210 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 217 210
BanII G_rGCy'C
Cuts at: 1391 3600 4051 4149 4187 4367 1391
Size: 2209 451 98 38 180 4072
Fragments arranged by size:
4072 2209 451 180 98 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 3296 928
Size: 971 125 1272 4680
Fragments arranged by size:
4680 1272 971 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4940 6150 4940
Size: 1210 5838
BclI T'GATC_A
Cuts at: 1548 2248 1548
Size: 700 6348
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4097 5659 136
Size: 107 122 71 631 605 2425 1562 1525
Fragments arranged by size:
2425 1562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7043 7043
Size: 7048
BlpI GC'TnA_GC
Cuts at: 4465 4465
Size: 7048
Bme1580I G_kGCm'C
Cuts at: 1790 4051 4187 5109 6265 1790
Size: 2261 136 922 1156 2573
Fragments arranged by size:
2573 2261 1156 922 136
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 2127 4779
Size: 416 53 83 399 24 690 20 2652
Cuts at: 4779 5737 442
Size: 958 1753
Fragments arranged by size:
2652 1753 958 690 416 399 83 53
24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3182 3298 3584 4136 4313 5708 1239
Size: 1943 116 286 552 177 1395 2579
Fragments arranged by size:
2579 1943 1395 552 286 177 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 5173 6038 6336 2902
Size: 2271 865 298 3614
Fragments arranged by size:
3614 2271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5711 882
Size: 617 4212 2219
Fragments arranged by size:
4212 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4839 493
Size: 711 3635 2702
Fragments arranged by size:
3635 2702 711
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4855 5237 5872 275
Size: 53 83 186 936 3322 382 635 1451
Fragments arranged by size:
3322 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5481 5879 6153 6300 6711 5481
Size: 398 274 147 411 5818
Fragments arranged by size:
5818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2632 2662 1178
Size: 30 165 30 1229 30 5564
Fragments arranged by size:
5564 1229 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7048
BseYI C'CCAG_C
Cuts at: 2037 4166 4187 4213 6251 6747 2037
Size: 2129 21 26 2038 496 2338
Fragments arranged by size:
2338 2129 2038 496 26 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2490 3149 4145 1163
Size: 573 754 659 996 4066
Fragments arranged by size:
4066 996 754 659 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4498 5259 6287 1540
Size: 275 186 2497 761 1028 2301
Fragments arranged by size:
2497 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 5109 5194 6265 3600
Size: 1509 85 1071 4383
Fragments arranged by size:
4383 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 4559 4652 4559
Size: 93 6955
BsmBI CGTCTCn'nnnn_
Cuts at: 3428 3428
Size: 7048
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3600 4051 4149 4187 4367 5109 5194
Size: 399 1810 451 98 38 180 742 85
Cuts at: 5194 6265 1391
Size: 1071 2174
Fragments arranged by size:
2174 1810 1071 742 451 399 180 98
85 38
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4403 844
Size: 3559 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4935 1394
Size: 429 3112 3507
Fragments arranged by size:
3507 3112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 2766 5543 5717 65
Size: 1183 1149 369 2777 174 1396
Fragments arranged by size:
2777 1396 1183 1149 369 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5692 5879 1700
Size: 110 3882 187 2869
Fragments arranged by size:
3882 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2657 96
Size: 2561 4487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 5108 6120 5108
Size: 1012 6036
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7048
BstBI TT'CG_AA
Cuts at: 5852 5852
Size: 7048
BstEII G'GTnAC_C
Cuts at: 1184 2363 1184
Size: 1179 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 3208 3208
Size: 7048
BstYI r'GATC_y
Cuts at: 1972 2782 2827 3351 3654 3772 4029 4378 4745
Size: 810 45 524 303 118 257 349 367
Cuts at: 4745 5130 5147 6599 6685 6697 7043 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 810 524 385 367 349 346
303 257 118 86 45 17 12
BstZ17I GTA'TAC
Cuts at: 2801 2801
Size: 7048
Bsu36I CC'TnA_GG
Cuts at: 4155 4155
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5557
Fragments arranged by size:
5557 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6309
Fragments arranged by size:
6309 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 3234 4564 5358 5378 2580
Size: 654 1330 794 20 4250
Fragments arranged by size:
4250 1330 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2489 2524 529
Size: 35 1925 35 5053
Fragments arranged by size:
5053 1925 35 35
DraI TTT'AAA
Cuts at: 3097 4352 3097
Size: 1255 5793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4495 5384 7013
Size: 54 1425 50 275 18 2665 889 1629
Cuts at: 7013 8
Size: 43
Fragments arranged by size:
2665 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4495 1537
Size: 275 2683 4090
Fragments arranged by size:
4090 2683 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 3034 3107 3573 4227 4978 1353
Size: 1197 484 73 466 654 751 3423
Fragments arranged by size:
3423 1197 751 654 484 466 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5635 6009 6155 387
Size: 1064 138 4046 374 146 1280
Fragments arranged by size:
4046 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3598 3598
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2832 3182 3298 3401 3584 3597 3857
Size: 393 1200 350 116 103 183 13 260
Cuts at: 3857 4018 4136 4313 5111 5708 6495 6763 1239
Size: 161 118 177 798 597 787 268 1524
Fragments arranged by size:
1524 1200 798 787 597 393 350 268
260 183 177 161 118 116 103 13
EcoO109I rG'GnC_Cy
Cuts at: 2513 4047 4183 4184 2513
Size: 1534 136 1 5377
Fragments arranged by size:
5377 1534 136 1
EcoRI G'AATT_C
Cuts at: 4357 4735 4357
Size: 378 6670
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 3321 6195 1678
Size: 1643 2874 2531
Fragments arranged by size:
2874 2531 1643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2372 2404 3538
Size: 32 135 32 716 32 52 32 1134
Cuts at: 3538 3557 3570 3589 3781 3813 5689 5721 5763
Size: 19 13 19 192 32 1876 32 42
Cuts at: 5763 5795 1373
Size: 32 2626
Fragments arranged by size:
2626 1876 1134 716 192 135 52 42
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3800 4392 4638 669
Size: 988 281 1862 592 246 3079
Fragments arranged by size:
3079 1862 988 592 281 246
HindIII A'AGCT_T
Cuts at: 748 2814 748
Size: 2066 4982
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 4373 4373
Size: 7048
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3114 3202 6162 6346 1305
Size: 1809 88 2960 184 2007
Fragments arranged by size:
2960 2007 1809 184 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5226
Fragments arranged by size:
5226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 5006 5365 5524 518
Size: 648 1003 2837 359 159 2042
Fragments arranged by size:
2837 2042 1003 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2570 4191 4464 5141 6289 6534 1606
Size: 964 1621 273 677 1148 245 2120
Fragments arranged by size:
2120 1621 1148 964 677 273 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5557
Fragments arranged by size:
5557 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 2029 2184 3151 3188 4406 5951 1132
Size: 97 800 155 967 37 1218 1545 2229
Fragments arranged by size:
2229 1545 1218 967 800 155 97 37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4069 1108
Size: 2961 4087
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2513 2513
Size: 7048
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 2124 4618 2124
Size: 2494 4554
PspOMI G'GGCC_C
Cuts at: 4047 4183 4047
Size: 136 6912
PstI C_TGCA'G
Cuts at: 830 2048 2575 3686 4201 4400 830
Size: 1218 527 1111 515 199 3478
Fragments arranged by size:
3478 1218 1111 527 515 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 2570 4191 2570
Size: 1621 5427
SacI G_AGCT'C
Cuts at: 3600 3600
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 3798 4390 1936
Size: 1862 592 4594
Fragments arranged by size:
4594 1862 592
SapI GCTCTTCn'nnn_
Cuts at: 3107 3573 4227 3107
Size: 466 654 5928
Fragments arranged by size:
5928 654 466
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SfcI C'TryA_G
Cuts at: 826 1045 2044 2571 2712 3682 4197 4396 5531
Size: 219 999 527 141 970 515 199 1135
Cuts at: 5531 6212 6403 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 999 970 681 527 515 219
199 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 4375 1872
Size: 2503 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2917 5152 6053 6315 820
Size: 197 926 974 2235 901 262 1553
Fragments arranged by size:
2235 1553 974 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 3151 3188 4406 1132
Size: 2019 37 1218 3774
Fragments arranged by size:
3774 2019 1218 37
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 3617 4153 1517
Size: 2100 536 4412
Fragments arranged by size:
4412 2100 536
StyI C'CwwG_G
Cuts at: 513 1065 2004 2987 3306 3357 4308 4487 513
Size: 552 939 983 319 51 951 179 3074
Fragments arranged by size:
3074 983 951 939 552 319 179 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5075 5092 5245 5430 5875 325
Size: 1255 3495 17 153 185 445 1498
Fragments arranged by size:
3495 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2657 5294 7006
Size: 274 80 33 51 1443 680 2637 1712
Cuts at: 7006 96
Size: 138
Fragments arranged by size:
2637 1712 1443 680 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 7008 7040 2042
Size: 32 4934 32 2050
Fragments arranged by size:
4934 2050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 4384 4384
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 4373 1870
Size: 2503 4545
XmnI GAAnn'nnTTC
Cuts at: 5177 5177
Size: 7048
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI