Restriction map for
pFN21AB8573
(Circular) (Six-base) MAPSORT of: pFN21AB8573.seq Check: 1533 from: 1 to: 7375
pFN21AB8573 7375 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5185 278
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 2739 4718 1937
Size: 268 534 1979 4594
Fragments arranged by size:
4594 1979 534 268
Acc65I G'GTAC_C
Cuts at: 2519 2759 4496 4696 2519
Size: 240 1737 200 5198
Fragments arranged by size:
5198 1737 240 200
AclI AA'CG_TT
Cuts at: 1563 5502 5875 1563
Size: 3939 373 3063
Fragments arranged by size:
3939 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2345 2627 3161 3575 4055 4146 4232
Size: 585 128 282 534 414 480 91 86
Cuts at: 4232 5438 6822 7090 1632
Size: 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 585 534 480 414 282
268 128 91 86
AfeI AGC'GCT
Cuts at: 3245 3245
Size: 7375
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7178
AflIII A'CryG_T
Cuts at: 1203 6274 7251 1203
Size: 5071 977 1327
Fragments arranged by size:
5071 1327 977
AgeI A'CCGG_T
Cuts at: 3354 6206 3354
Size: 2852 4523
AhdI GACnn_n'nnGTC
Cuts at: 6104 6104
Size: 7375
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2135 1184
Size: 951 6424
AlwNI CAG_nnn'CTG
Cuts at: 2867 3370 4013 4052 4369 5118 6690 2867
Size: 503 643 39 317 749 1572 3552
Fragments arranged by size:
3552 1572 749 643 503 317 39
ApaLI G'TGCA_C
Cuts at: 5432 6588 5432
Size: 1156 6219
ApoI r'AATT_y
Cuts at: 1493 3777 4558 4684 4910 5062 7171 1493
Size: 2284 781 126 226 152 2109 1697
Fragments arranged by size:
2284 2109 1697 781 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 5929 160
Size: 872 4897 1606
Fragments arranged by size:
4897 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
AvaI C'yCGr_G
Cuts at: 1870 1943 3399 4700 1870
Size: 73 1456 1301 4545
Fragments arranged by size:
4545 1456 1301 73
BamHI G'GATC_C
Cuts at: 2016 3165 4617 4705 5072 2016
Size: 1149 1452 88 367 4319
Fragments arranged by size:
4319 1452 1149 367 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2519 2759 4496 4696 6151
Size: 325 217 627 732 240 1737 200 1455
Cuts at: 6151 618
Size: 1842
Fragments arranged by size:
1842 1737 1455 732 627 325 240 217
200
BanII G_rGCy'C
Cuts at: 1391 2796 3693 4694 1391
Size: 1405 897 1001 4072
Fragments arranged by size:
4072 1405 1001 897
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3581 3722 928
Size: 971 1682 141 4581
Fragments arranged by size:
4581 1682 971 141
BbvCI CC'TCA_GC
Cuts at: 3060 4168 3060
Size: 1108 6267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2755 2789 5566 5600 1903
Size: 34 818 34 2777 34 3678
Fragments arranged by size:
3678 2777 818 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3607 5267 6477 3607
Size: 1660 1210 4505
Fragments arranged by size:
4505 1660 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7375
BfrBI ATG'CAT
Cuts at: 3763 3763
Size: 7375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2580 5986 136
Size: 107 122 71 631 605 908 3406 1525
Fragments arranged by size:
3406 1525 908 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2379 2925 3031 7370 2379
Size: 546 106 4339 2384
Fragments arranged by size:
4339 2384 546 106
BlpI GC'TnA_GC
Cuts at: 4437 4792 4437
Size: 355 7020
Bme1580I G_kGCm'C
Cuts at: 1790 2857 5436 6592 1790
Size: 1067 2579 1156 2573
Fragments arranged by size:
2579 2573 1156 1067
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7375
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3557 4311 5106
Size: 416 53 83 399 24 2140 754 795
Cuts at: 5106 6064 442
Size: 958 1753
Fragments arranged by size:
2140 1753 958 795 754 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3519 1056
Size: 2463 4912
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 4132 2558
Size: 1574 5801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3118 3776 6035 1239
Size: 1879 658 2259 2579
Fragments arranged by size:
2579 2259 1879 658
Bpu10I CC'TnA_GC
Cuts at: 3060 3621 4168 3060
Size: 561 547 6267
Fragments arranged by size:
6267 561 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 3875 5500 6365 6663 2230
Size: 1645 1625 865 298 2942
Fragments arranged by size:
2942 1645 1625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 6038 882
Size: 617 780 3759 2219
Fragments arranged by size:
3759 2219 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 5166 493
Size: 711 3962 2702
Fragments arranged by size:
3962 2702 711
BsaBI GATnn'nnATC
Cuts at: 3556 7369 3556
Size: 3813 3562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5182 5564 6199 275
Size: 53 83 186 936 3649 382 635 1451
Fragments arranged by size:
3649 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3354 5808 6206 6480 6627 7038 3354
Size: 2454 398 274 147 411 3691
Fragments arranged by size:
3691 2454 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 3116 3146 3180
Size: 30 165 30 1296 30 387 30 34
Cuts at: 3180 3210 3851 3881 1178
Size: 30 641 30 4672
Fragments arranged by size:
4672 1296 641 387 165 34 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 2723 2746 3077 3701 1186
Size: 1534 3 23 331 624 4860
Fragments arranged by size:
4860 1534 624 331 23 3
BseYI C'CCAG_C
Cuts at: 2789 3602 4062 6578 7074 2789
Size: 813 460 2516 496 3090
Fragments arranged by size:
3090 2516 813 496 460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3548 3844 1163
Size: 573 1812 296 4694
Fragments arranged by size:
4694 1812 573 296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4825 5586 6614 1540
Size: 275 186 2824 761 1028 2301
Fragments arranged by size:
2824 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3693 5436 5521 6592 3693
Size: 1743 85 1071 4476
Fragments arranged by size:
4476 1743 1071 85
BsmI GAATG_Cn'
Cuts at: 4886 4979 4886
Size: 93 7282
BsmBI CGTCTCn'nnnn_
Cuts at: 2146 2146
Size: 7375
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2796 2857 3693 4694 5436 5521 6592
Size: 399 1006 61 836 1001 742 85 1071
Cuts at: 6592 1391
Size: 2174
Fragments arranged by size:
2174 1071 1006 1001 836 742 399 85
61
BspEI T'CCGG_A
Cuts at: 7038 7038
Size: 7375
BspHI T'CATG_A
Cuts at: 5264 6994 5264
Size: 1730 5645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2158 4589 4730 844
Size: 1283 31 2431 141 3489
Fragments arranged by size:
3489 2431 1283 141 31
BsrBI CCG'CTC
Cuts at: 1394 1823 3934 5262 1394
Size: 429 2111 1328 3507
Fragments arranged by size:
3507 2111 1328 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2338 2784 3510 3790 5870 6044 65
Size: 1183 1090 446 726 280 2080 174 1396
Fragments arranged by size:
2080 1396 1183 1090 726 446 280 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3354 6019 6206 1700
Size: 110 1544 2665 187 2869
Fragments arranged by size:
2869 2665 1544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7375
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7375
BssSI C'ACGA_G
Cuts at: 5435 6447 5435
Size: 1012 6363
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2989 4052 4433 794
Size: 2195 1063 381 3736
Fragments arranged by size:
3736 2195 1063 381
BstBI TT'CG_AA
Cuts at: 4555 6179 4555
Size: 1624 5751
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7375
BstXI CCAn_nnnn'nTGG
Cuts at: 2384 4192 4301 2384
Size: 1808 109 5458
Fragments arranged by size:
5458 1808 109
BstYI r'GATC_y
Cuts at: 1972 2016 2379 2652 2910 2925 3031 3165 4617
Size: 44 363 273 258 15 106 134 1452
Cuts at: 4617 4705 5072 5457 5474 6926 7012 7024 7370
Size: 88 367 385 17 1452 86 12 346
Cuts at: 7370 1972
Size: 1977
Fragments arranged by size:
1977 1452 1452 385 367 363 346 273
258 134 106 88 86 44 17 15
12
BstZ17I GTA'TAC
Cuts at: 2206 2206
Size: 7375
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6636
Fragments arranged by size:
6636 683 56
BtsI GCAGTG_nn'
Cuts at: 2865 3719 3832 4103 4891 5685 5705 2865
Size: 854 113 271 788 794 20 4535
Fragments arranged by size:
4535 854 794 788 271 113 20
ClaI AT'CG_AT
Cuts at: 1554 2848 1554
Size: 1294 6081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7340
DraI TTT'AAA
Cuts at: 4679 4679
Size: 7375
DraIII CAC_nnn'GTG
Cuts at: 3224 3224
Size: 7375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 6382 809
Size: 507 819 4247 1802
Fragments arranged by size:
4247 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 3495 4822
Size: 54 1425 50 275 18 517 1148 1327
Cuts at: 4822 5711 7340 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1327 1148 889 517 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4822 1537
Size: 275 3010 4090
Fragments arranged by size:
4090 3010 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2723 2803 3254 3492 3915 3945 4122
Size: 968 402 80 451 238 423 30 177
Cuts at: 4122 4203 5305 1353
Size: 81 1102 3423
Fragments arranged by size:
3423 1102 968 451 423 402 238 177
81 80 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2016 5962 6336 6482 387
Size: 1064 138 427 3946 374 146 1280
Fragments arranged by size:
3946 1280 1064 427 374 146 138
EcoICRI GAG'CTC
Cuts at: 3691 3691
Size: 7375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2345 2627 3118 3161 3575 3776
Size: 393 585 128 282 491 43 414 201
Cuts at: 3776 4055 4146 4232 5438 6035 6822 7090 1239
Size: 279 91 86 1206 597 787 268 1524
Fragments arranged by size:
1524 1206 787 597 585 491 414 393
282 279 268 201 128 91 86 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2728 2702
Size: 26 7349
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 7375
EcoRI G'AATT_C
Cuts at: 4684 5062 4684
Size: 378 6997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2036 2603 2036
Size: 567 6808
FspI TGC'GCA
Cuts at: 5881 5881
Size: 7375
HaeII r_GCGC'y
Cuts at: 1678 2561 3247 4492 6522 1678
Size: 883 686 1245 2030 2531
Fragments arranged by size:
2531 2030 1245 883 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2084 2116 2699
Size: 32 135 32 436 32 44 32 583
Cuts at: 2699 2731 3180 3212 6016 6048 6090 6122 1373
Size: 32 449 32 2804 32 42 32 2626
Fragments arranged by size:
2804 2626 583 449 436 135 44 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 4719 4965 669
Size: 988 281 1458 1323 246 3079
Fragments arranged by size:
3079 1458 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7375
HpaI GTT'AAC
Cuts at: 4965 4965
Size: 7375
KpnI G_GTAC'C
Cuts at: 2523 2763 4500 4700 2523
Size: 240 1737 200 5198
Fragments arranged by size:
5198 1737 240 200
MfeI C'AATT_G
Cuts at: 4974 4974
Size: 7375
MluI A'CGCG_T
Cuts at: 7251 7251
Size: 7375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 2618 2680 2732 6489 6673 1305
Size: 915 398 62 52 3757 184 2007
Fragments arranged by size:
3757 2007 915 398 184 62 52
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 3497 10
Size: 54 1425 343 517 1148 3888
Fragments arranged by size:
3888 1425 1148 517 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3671 4190 5333 5692 5851 518
Size: 648 2505 519 1143 359 159 2042
Fragments arranged by size:
2505 2042 1143 648 519 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2087 2830 3321 3754 3811 4791 5468
Size: 427 54 743 491 433 57 980 677
Cuts at: 5468 6616 6861 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 980 743 677 491 433 427
245 57 54
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6823
NdeI CA'TA_TG
Cuts at: 387 2479 387
Size: 2092 5283
NheI G'CTAG_C
Cuts at: 1052 3515 1052
Size: 2463 4912
NotI GC'GGCC_GC
Cuts at: 4822 4822
Size: 7375
NsiI A_TGCA'T
Cuts at: 3765 3765
Size: 7375
NspI r_CATG'y
Cuts at: 1132 1229 3533 4431 4733 6278 1132
Size: 97 2304 898 302 1545 2229
Fragments arranged by size:
2304 2229 1545 898 302 97
PasI CC'CwG_GG
Cuts at: 1348 3313 1348
Size: 1965 5410
PciI A'CATG_T
Cuts at: 6274 6274
Size: 7375
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4369 1108
Size: 3261 4114
PmeI GTTT'AAAC
Cuts at: 4679 4679
Size: 7375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5444 5476 6957 6989 5444
Size: 32 1481 32 5830
Fragments arranged by size:
5830 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3985 1613
Size: 2372 5003
PsiI TTA'TAA
Cuts at: 4945 4945
Size: 7375
PspXI vC'TCGA_Gb
Cuts at: 3399 3399
Size: 7375
PstI C_TGCA'G
Cuts at: 830 2092 4383 4604 4727 830
Size: 1262 2291 221 123 3478
Fragments arranged by size:
3478 2291 1262 221 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7375
PvuII CAG'CTG
Cuts at: 2087 2830 3754 2087
Size: 743 924 5708
Fragments arranged by size:
5708 924 743
SacI G_AGCT'C
Cuts at: 3693 3693
Size: 7375
SalI G'TCGA_C
Cuts at: 1936 4717 1936
Size: 2781 4594
SapI GCTCTTCn'nnn_
Cuts at: 2321 3945 2321
Size: 1624 5751
SbfI CC_TGCA'GG
Cuts at: 4727 4727
Size: 7375
ScaI AGT'ACT
Cuts at: 5623 7335 5623
Size: 1712 5663
SexAI A'CCwGG_T
Cuts at: 3369 3369
Size: 7375
SfcI C'TryA_G
Cuts at: 826 1045 2088 3749 4379 4600 4723 5858 6539
Size: 219 1043 1661 630 221 123 1135 681
Cuts at: 6539 6730 826
Size: 191 1471
Fragments arranged by size:
1661 1471 1135 1043 681 630 221 219
191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7375
SmaI CCC'GGG
Cuts at: 1872 4702 1872
Size: 2830 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 3399 3890 5479 6380 6642
Size: 197 926 266 1190 491 1589 901 262
Cuts at: 6642 820
Size: 1553
Fragments arranged by size:
1589 1553 1190 926 901 491 266 262
197
SnaBI TAC'GTA
Cuts at: 493 5166 493
Size: 4673 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7375
SphI G_CATG'C
Cuts at: 1132 3533 4733 1132
Size: 2401 1200 3774
Fragments arranged by size:
3774 2401 1200
SspI AAT'ATT
Cuts at: 5 52 5299 5
Size: 47 5247 2081
Fragments arranged by size:
5247 2081 47
StuI AGG'CCT
Cuts at: 1517 2697 3068 3636 1517
Size: 1180 371 568 5256
Fragments arranged by size:
5256 1180 568 371
StyI C'CwwG_G
Cuts at: 513 1065 2307 2578 3409 4465 4492 4814 513
Size: 552 1242 271 831 1056 27 322 3074
Fragments arranged by size:
3074 1242 1056 831 552 322 271 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 3131 3857 4076 5402 5419 5572
Size: 1255 740 811 726 219 1326 17 153
Cuts at: 5572 5757 6202 325
Size: 185 445 1498
Fragments arranged by size:
1498 1326 1255 811 740 726 445 219
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2446 5621 7333
Size: 274 80 33 51 1443 469 3175 1712
Cuts at: 7333 96
Size: 138
Fragments arranged by size:
3175 1712 1443 469 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2865 2897 3806 3838 7335 7367 2865
Size: 32 909 32 3497 32 2873
Fragments arranged by size:
3497 2873 909 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2054 1316
Size: 738 6637
XbaI T'CTAG_A
Cuts at: 4711 4711
Size: 7375
XhoI C'TCGA_G
Cuts at: 1943 3399 1943
Size: 1456 5919
XmaI C'CCGG_G
Cuts at: 1870 4700 1870
Size: 2830 4545
XmnI GAAnn'nnTTC
Cuts at: 3486 3924 4547 5504 3486
Size: 438 623 957 5357
Fragments arranged by size:
5357 957 623 438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5183 276
Size: 53 83 186 936 3649 2468
Fragments arranged by size:
3649 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI