Restriction map for
pFN21AB8571
(Circular) (Six-base) MAPSORT of: pFN21AB8571.seq Check: 3725 from: 1 to: 6424
pFN21AB8571 6424 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 4234 278
Size: 53 83 186 936 2086 612 2468
Fragments arranged by size:
2468 2086 936 612 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3767 1937
Size: 1830 4594
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 4551 4924 1563
Size: 2988 373 3063
Fragments arranged by size:
3063 2988 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 4487 5871 6139 1632
Size: 855 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 855 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 2921 3713 5323 6300 1203
Size: 1718 792 1610 977 1327
Fragments arranged by size:
1718 1610 1327 977 792
AgeI A'CCGG_T
Cuts at: 5255 5255
Size: 6424
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3587 1184
Size: 2403 4021
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 6392
AlwNI CAG_nnn'CTG
Cuts at: 2510 2807 3356 4167 5739 2510
Size: 297 549 811 1572 3195
Fragments arranged by size:
3195 1572 811 549 297
ApaLI G'TGCA_C
Cuts at: 4481 5637 4481
Size: 1156 5268
ApoI r'AATT_y
Cuts at: 1493 2236 3733 3959 4111 6220 1493
Size: 743 1497 226 152 2109 1697
Fragments arranged by size:
2109 1697 1497 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3212 4978 160
Size: 872 2180 1766 1606
Fragments arranged by size:
2180 1766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 3749 1870
Size: 73 1281 525 4545
Fragments arranged by size:
4545 1281 525 73
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3285 3745 5200 618
Size: 325 217 627 1498 460 1455 1842
Fragments arranged by size:
1842 1498 1455 627 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2861 3249 3296 3332 3408 3743 1391
Size: 1470 388 47 36 76 335 4072
Fragments arranged by size:
4072 1470 388 335 76 47 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 3371 3692 928
Size: 971 383 1089 321 3660
Fragments arranged by size:
3660 1089 971 383 321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4615 4649 1903
Size: 34 2678 34 3678
Fragments arranged by size:
3678 2678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4316 5526 3578
Size: 738 1210 4476
Fragments arranged by size:
4476 1210 738
BclI T'GATC_A
Cuts at: 1548 2116 1548
Size: 568 5856
BfrBI ATG'CAT
Cuts at: 3672 3672
Size: 6424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5035 136
Size: 107 122 71 631 605 3363 1525
Fragments arranged by size:
3363 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6419 6419
Size: 6424
BlpI GC'TnA_GC
Cuts at: 3841 3841
Size: 6424
Bme1580I G_kGCm'C
Cuts at: 1790 4485 5641 1790
Size: 2695 1156 2573
Fragments arranged by size:
2695 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2922 1318
Size: 1604 4820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 3702 4155
Size: 416 53 83 399 24 1874 411 453
Cuts at: 4155 5113 442
Size: 958 1753
Fragments arranged by size:
1874 1753 958 453 416 411 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2532 2799 3263 3410 3628 5084 1239
Size: 1293 267 464 147 218 1456 2579
Fragments arranged by size:
2579 1456 1293 464 267 218 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3027 4549 5414 5712 3027
Size: 1522 865 298 3739
Fragments arranged by size:
3739 1522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5087 882
Size: 617 3588 2219
Fragments arranged by size:
3588 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2156 4215 493
Size: 711 952 2059 2702
Fragments arranged by size:
2702 2059 952 711
BsaBI GATnn'nnATC
Cuts at: 2068 6418 2068
Size: 4350 2074
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4231 4613 5248
Size: 53 83 186 936 2086 612 382 635
Cuts at: 5248 275
Size: 1451
Fragments arranged by size:
2086 1451 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4857 5255 5529 5676 6087 4857
Size: 398 274 147 411 5194
Fragments arranged by size:
5194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2797 2827 3649 3679 1178
Size: 30 165 30 1394 30 822 30 3923
Fragments arranged by size:
3923 1394 822 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3312 1186
Size: 2126 4298
BseYI C'CCAG_C
Cuts at: 3309 5627 6123 3309
Size: 2318 496 3610
Fragments arranged by size:
3610 2318 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5851
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 3408 3659 4485 4570 5641 2861
Size: 547 251 826 85 1071 3644
Fragments arranged by size:
3644 1071 826 547 251 85
BsmI GAATG_Cn'
Cuts at: 3466 3935 4028 3466
Size: 469 93 5862
Fragments arranged by size:
5862 469 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2912 3077 2912
Size: 165 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2861 3249 3296 3332 3408 3659 3743
Size: 399 1071 388 47 36 76 251 84
Cuts at: 3743 4485 4570 5641 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1071 742 399 388 251 85
84 76 47 36
BspEI T'CCGG_A
Cuts at: 6087 6087
Size: 6424
BspHI T'CATG_A
Cuts at: 2985 4313 6043 2985
Size: 1328 1730 3366
Fragments arranged by size:
3366 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3779 844
Size: 2935 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 4311 1394
Size: 429 1882 606 3507
Fragments arranged by size:
3507 1882 606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 3471 4919 5093 65
Size: 1183 1504 719 1448 174 1396
Fragments arranged by size:
1504 1448 1396 1183 719 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5068 5255 1700
Size: 110 3258 187 2869
Fragments arranged by size:
3258 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2593 96
Size: 2497 3927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6424
BstBI TT'CG_AA
Cuts at: 5228 5228
Size: 6424
BstEII G'GTnAC_C
Cuts at: 1184 3416 1184
Size: 2232 4192
BstYI r'GATC_y
Cuts at: 1972 3754 4121 4506 4523 5975 6061 6073 6419
Size: 1782 367 385 17 1452 86 12 346
Cuts at: 6419 1972
Size: 1977
Fragments arranged by size:
1977 1782 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2992 513
Size: 552 1927 3945
Fragments arranged by size:
3945 1927 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5685
Fragments arranged by size:
5685 683 56
BtsI GCAGTG_nn'
Cuts at: 2285 3940 4734 4754 2285
Size: 1655 794 20 3955
Fragments arranged by size:
3955 1655 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6389
DraI TTT'AAA
Cuts at: 2059 3728 2059
Size: 1669 4755
DraIII CAC_nnn'GTG
Cuts at: 3285 3285
Size: 6424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5431 809
Size: 507 4115 1802
Fragments arranged by size:
4115 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3871 4760 6389
Size: 54 1425 50 275 18 2041 889 1629
Cuts at: 6389 8
Size: 43
Fragments arranged by size:
2041 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3375 3542 3581 3647 4354 1353
Size: 2022 167 39 66 707 3423
Fragments arranged by size:
3423 2022 707 167 66 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5011 5385 5531 387
Size: 1064 138 3422 374 146 1280
Fragments arranged by size:
3422 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2859 3406 2859
Size: 547 5877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2532 2799 3263 3410 3628 4487
Size: 393 855 45 267 464 147 218 859
Cuts at: 4487 5084 5871 6139 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 859 855 787 597 464 393 268
267 218 147 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2478 2478
Size: 6424
EcoRI G'AATT_C
Cuts at: 3733 4111 3733
Size: 378 6046
FspI TGC'GCA
Cuts at: 4930 4930
Size: 6424
HaeII r_GCGC'y
Cuts at: 1678 5571 1678
Size: 3893 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2762 2794 3236
Size: 32 135 32 427 32 731 32 442
Cuts at: 3236 3268 3576 3608 3689 3721 5065 5097 5139
Size: 32 308 32 81 32 1344 32 42
Cuts at: 5139 5171 1373
Size: 32 2626
Fragments arranged by size:
2626 1344 731 442 427 308 135 81
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3768 4014 669
Size: 988 281 1830 246 3079
Fragments arranged by size:
3079 1830 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6424
HpaI GTT'AAC
Cuts at: 4014 4014
Size: 6424
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 4023 4023
Size: 6424
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 3437 5538 5722 1305
Size: 1072 1060 2101 184 2007
Fragments arranged by size:
2101 2007 1072 1060 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4602
Fragments arranged by size:
4602 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2997 3189 3569 3677 4382 4741 4900
Size: 648 1831 192 380 108 705 359 159
Cuts at: 4900 518
Size: 2042
Fragments arranged by size:
2042 1831 705 648 380 359 192 159
108
MspA1I CmG'CkG
Cuts at: 1606 2294 3353 3378 3840 4517 5665 5910 1606
Size: 688 1059 25 462 677 1148 245 2120
Fragments arranged by size:
2120 1148 1059 688 677 462 245 25
NcoI C'CATG_G
Cuts at: 513 1065 2992 513
Size: 552 1927 3945
Fragments arranged by size:
3945 1927 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NsiI A_TGCA'T
Cuts at: 3674 3674
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 3717 3782 5327 1132
Size: 97 2488 65 1545 2229
Fragments arranged by size:
2488 2229 1545 97 65
PacI TTA_AT'TAA
Cuts at: 2193 2193
Size: 6424
PasI CC'CwG_GG
Cuts at: 1348 2908 3496 1348
Size: 1560 588 4276
Fragments arranged by size:
4276 1560 588
PciI A'CATG_T
Cuts at: 3713 5323 3713
Size: 1610 4814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 2881 3674 1108
Size: 1189 584 793 3858
Fragments arranged by size:
3858 1189 793 584
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PmlI CAC'GTG
Cuts at: 2156 2156
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6424
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PstI C_TGCA'G
Cuts at: 830 3352 3776 830
Size: 2522 424 3478
Fragments arranged by size:
3478 2522 424
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
PvuII CAG'CTG
Cuts at: 2294 3353 3378 2294
Size: 1059 25 5340
Fragments arranged by size:
5340 1059 25
SacI G_AGCT'C
Cuts at: 2861 3408 2861
Size: 547 5877
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SapI GCTCTTCn'nnn_
Cuts at: 3581 3647 3581
Size: 66 6358
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 4672 6384 4672
Size: 1712 4712
SfcI C'TryA_G
Cuts at: 826 1045 3348 3772 4907 5588 5779 826
Size: 219 2303 424 1135 681 191 1471
Fragments arranged by size:
2303 1471 1135 681 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6424
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3042 4528 5429 5691 820
Size: 197 926 1099 1486 901 262 1553
Fragments arranged by size:
1553 1486 1099 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4215 493
Size: 3722 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6424
SphI G_CATG'C
Cuts at: 1132 3782 1132
Size: 2650 3774
SspI AAT'ATT
Cuts at: 5 52 2570 4348 5
Size: 47 2518 1778 2081
Fragments arranged by size:
2518 2081 1778 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6424
StyI C'CwwG_G
Cuts at: 513 1065 2412 2992 3421 3863 513
Size: 552 1347 580 429 442 3074
Fragments arranged by size:
3074 1347 580 552 442 429
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 3273 4451 4468 4621 4806 5251
Size: 1255 455 1238 1178 17 153 185 445
Cuts at: 5251 325
Size: 1498
Fragments arranged by size:
1498 1255 1238 1178 455 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2593 4670 6382
Size: 274 80 33 51 1443 616 2077 1712
Cuts at: 6382 96
Size: 138
Fragments arranged by size:
2077 1712 1443 616 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3417 3449 3649 3681 6384 6416 3417
Size: 32 200 32 2703 32 3425
Fragments arranged by size:
3425 2703 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6424
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6424
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 2569 4553 2569
Size: 1984 4440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 4232 276
Size: 53 83 186 936 2086 612 2468
Fragments arranged by size:
2468 2086 936 612 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI