Restriction map for
pFN21AB8569
(Circular) (Six-base) MAPSORT of: pFN21AB8569.seq Check: 1861 from: 1 to: 7018
pFN21AB8569 7018 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4828 278
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4361 1937
Size: 2424 4594
Acc65I G'GTAC_C
Cuts at: 4339 4339
Size: 7018
AclI AA'CG_TT
Cuts at: 1563 5145 5518 1563
Size: 3582 373 3063
Fragments arranged by size:
3582 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2398 2858 3380 3731 3803 5081 6465
Size: 465 301 460 522 351 72 1278 1384
Cuts at: 6465 6733 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1278 522 465 460 351 301
268 72
AfeI AGC'GCT
Cuts at: 2990 2990
Size: 7018
AflII C'TTAA_G
Cuts at: 820 1017 3788 820
Size: 197 2771 4050
Fragments arranged by size:
4050 2771 197
AflIII A'CryG_T
Cuts at: 1203 5917 6894 1203
Size: 4714 977 1327
Fragments arranged by size:
4714 1327 977
AgeI A'CCGG_T
Cuts at: 4086 5849 4086
Size: 1763 5255
AhdI GACnn_n'nnGTC
Cuts at: 5747 5747
Size: 7018
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 3324 4032 1184
Size: 1500 640 708 4170
Fragments arranged by size:
4170 1500 708 640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 6986
AlwNI CAG_nnn'CTG
Cuts at: 2036 2075 2133 2298 2357 2591 2864 3908 4761
Size: 39 58 165 59 234 273 1044 853
Cuts at: 4761 6333 2036
Size: 1572 2721
Fragments arranged by size:
2721 1572 1044 853 273 234 165 59
58 39
ApaI G_GGCC'C
Cuts at: 2569 2768 2569
Size: 199 6819
ApaLI G'TGCA_C
Cuts at: 5075 6231 5075
Size: 1156 5862
ApoI r'AATT_y
Cuts at: 1493 3626 4327 4553 4705 6814 1493
Size: 2133 701 226 152 2109 1697
Fragments arranged by size:
2133 2109 1697 701 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5572 160
Size: 872 4540 1606
Fragments arranged by size:
4540 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 2975 3073 4343 1870
Size: 73 444 588 98 1270 4545
Fragments arranged by size:
4545 1270 588 444 98 73
BamHI G'GATC_C
Cuts at: 3219 3852 4348 4715 3219
Size: 633 496 367 5522
Fragments arranged by size:
5522 633 496 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2459 2922 3823 4339
Size: 325 217 627 372 300 463 901 516
Cuts at: 4339 5794 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 901 627 516 463 372 325
300 217
BanII G_rGCy'C
Cuts at: 1391 2569 2768 2871 4172 4337 1391
Size: 1178 199 103 1301 165 4072
Fragments arranged by size:
4072 1301 1178 199 165 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3368 3575 4244 928
Size: 971 1469 207 669 3702
Fragments arranged by size:
3702 1469 971 669 207
BbvCI CC'TCA_GC
Cuts at: 2216 2358 2367 3422 4259 2216
Size: 142 9 1055 837 4975
Fragments arranged by size:
4975 1055 837 142 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5209 5243 1903
Size: 34 3272 34 3678
Fragments arranged by size:
3678 3272 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4910 6120 4910
Size: 1210 5808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7018
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2763 3749 4202
Size: 107 122 71 631 605 1091 986 453
Cuts at: 4202 5629 136
Size: 1427 1525
Fragments arranged by size:
1525 1427 1091 986 631 605 453 122
107 71
BglII A'GATC_T
Cuts at: 7013 7013
Size: 7018
BlpI GC'TnA_GC
Cuts at: 2495 2870 4435 2495
Size: 375 1565 5078
Fragments arranged by size:
5078 1565 375
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2162 2462 2569 2768 3241 5079 6235
Size: 358 14 300 107 199 473 1838 1156
Cuts at: 6235 1790
Size: 2573
Fragments arranged by size:
2573 1838 1156 473 358 300 199 107
14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7018
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 4040 4314
Size: 416 53 83 399 24 1041 1582 274
Cuts at: 4314 4749 5707 442
Size: 435 958 1753
Fragments arranged by size:
1753 1582 1041 958 435 416 399 274
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2320 2459 2668 3823 4202 5678 1239
Size: 1081 139 209 1155 379 1476 2579
Fragments arranged by size:
2579 1476 1155 1081 379 209 139
Bpu10I CC'TnA_GC
Cuts at: 2216 2358 2367 2580 2708 3422 4259 2216
Size: 142 9 213 128 714 837 4975
Fragments arranged by size:
4975 837 714 213 142 128 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5143 6008 6306 5143
Size: 865 298 5855
Fragments arranged by size:
5855 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3993 4124 5681 882
Size: 617 2494 131 1557 2219
Fragments arranged by size:
2494 2219 1557 617 131
BsaAI yAC'GTr
Cuts at: 493 1204 4809 493
Size: 711 3605 2702
Fragments arranged by size:
3605 2702 711
BsaBI GATnn'nnATC
Cuts at: 7012 7012
Size: 7018
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3555 3599 4825 5207
Size: 53 83 186 936 2022 44 1226 382
Cuts at: 5207 5842 275
Size: 635 1451
Fragments arranged by size:
2022 1451 1226 936 635 382 186 83
53 44
BsaWI w'CCGG_w
Cuts at: 2094 4086 4233 5451 5849 6123 6270 6681 2094
Size: 1992 147 1218 398 274 147 411 2431
Fragments arranged by size:
2431 1992 1218 411 398 274 147 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4073 4103 1178
Size: 30 165 30 2670 30 4093
Fragments arranged by size:
4093 2670 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2096 2807 3989 4094 1186
Size: 838 72 711 1182 105 4110
Fragments arranged by size:
4110 1182 838 711 105 72
BseYI C'CCAG_C
Cuts at: 2164 2371 2591 2656 2831 3010 3901 6221 6717
Size: 207 220 65 175 179 891 2320 496
Cuts at: 6717 2164
Size: 2465
Fragments arranged by size:
2465 2320 891 496 220 207 179 175
65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2922 3248 3937 1163
Size: 573 1186 326 689 4244
Fragments arranged by size:
4244 1186 689 573 326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4468 5229 6257 1540
Size: 275 186 2467 761 1028 2301
Fragments arranged by size:
2467 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2871 3097 3642 4101 5079 5164 6235 2871
Size: 226 545 459 978 85 1071 3654
Fragments arranged by size:
3654 1071 978 545 459 226 85
BsmI GAATG_Cn'
Cuts at: 4529 4622 4529
Size: 93 6925
BsmBI CGTCTCn'nnnn_
Cuts at: 2969 2969
Size: 7018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2162 2462 2569 2768 2871 3097
Size: 399 358 14 300 107 199 103 226
Cuts at: 3097 3241 3642 4101 4172 4337 5079 5164 6235
Size: 144 401 459 71 165 742 85 1071
Cuts at: 6235 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 459 401 399 358 300
226 199 165 144 107 103 85 71
14
BspEI T'CCGG_A
Cuts at: 2094 4233 6681 2094
Size: 2139 2448 2431
Fragments arranged by size:
2448 2431 2139
BspHI T'CATG_A
Cuts at: 3991 4907 6637 3991
Size: 916 1730 4372
Fragments arranged by size:
4372 1730 916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3270 3363 3669 4373 844
Size: 2426 93 306 704 3489
Fragments arranged by size:
3489 2426 704 306 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2694 3151 4186 4905 1394
Size: 429 871 457 1035 719 3507
Fragments arranged by size:
3507 1035 871 719 457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3306 5513 5687 65
Size: 1183 2058 2207 174 1396
Fragments arranged by size:
2207 2058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3174 4086 5662 5849 1700
Size: 110 1364 912 1576 187 2869
Fragments arranged by size:
2869 1576 1364 912 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7018
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7018
BssSI C'ACGA_G
Cuts at: 2147 5078 6090 2147
Size: 2931 1012 3075
Fragments arranged by size:
3075 2931 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2133 794
Size: 1339 5679
BstBI TT'CG_AA
Cuts at: 5822 5822
Size: 7018
BstEII G'GTnAC_C
Cuts at: 1184 3704 1184
Size: 2520 4498
BstXI CCAn_nnnn'nTGG
Cuts at: 2753 3278 4277 2753
Size: 525 999 5494
Fragments arranged by size:
5494 999 525
BstYI r'GATC_y
Cuts at: 1972 3219 3852 4348 4715 5100 5117 6569 6655
Size: 1247 633 496 367 385 17 1452 86
Cuts at: 6655 6667 7013 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1247 633 496 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2037 2760 3857 2037
Size: 723 1097 5198
Fragments arranged by size:
5198 1097 723
BtgI C'CryG_G
Cuts at: 513 1065 2004 2209 3604 513
Size: 552 939 205 1395 3927
Fragments arranged by size:
3927 1395 939 552 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2061 487
Size: 683 56 835 5444
Fragments arranged by size:
5444 835 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 3642 4534 5328 5348 2037
Size: 1605 892 794 20 3707
Fragments arranged by size:
3707 1605 892 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6983
DraI TTT'AAA
Cuts at: 4322 4322
Size: 7018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 6025 809
Size: 507 1206 3503 1802
Fragments arranged by size:
3503 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2727 2913
Size: 54 1425 50 275 18 276 621 186
Cuts at: 2913 3042 3268 3334 3741 4026 4203 4465 5354
Size: 129 226 66 407 285 177 262 889
Cuts at: 5354 6983 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 621 407 285 276 275
262 226 186 177 129 66 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4465 1537
Size: 275 2653 4090
Fragments arranged by size:
4090 2653 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3821 4948 1353
Size: 720 1748 1127 3423
Fragments arranged by size:
3423 1748 1127 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5605 5979 6125 387
Size: 1064 138 4016 374 146 1280
Fragments arranged by size:
4016 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 7018
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2320 2398 2459 2668 2858 3380
Size: 393 465 223 78 61 209 190 522
Cuts at: 3380 3731 3803 3823 4202 5081 5678 6465 6733
Size: 351 72 20 379 879 597 787 268
Cuts at: 6733 1239
Size: 1524
Fragments arranged by size:
1524 879 787 597 522 465 393 379
351 268 223 209 190 78 72 61
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2040 2583 3665 3818 3968 4262 2040
Size: 543 1082 153 150 294 4796
Fragments arranged by size:
4796 1082 543 294 153 150
EcoO109I rG'GnC_Cy
Cuts at: 2116 2191 2295 2362 2536 2764 3154 3750 2116
Size: 75 104 67 174 228 390 596 5384
Fragments arranged by size:
5384 596 390 228 174 104 75 67
EcoRI G'AATT_C
Cuts at: 4327 4705 4327
Size: 378 6640
FspI TGC'GCA
Cuts at: 5524 5524
Size: 7018
HaeII r_GCGC'y
Cuts at: 1678 2421 2677 2992 3530 6165 1678
Size: 743 256 315 538 2635 2531
Fragments arranged by size:
2635 2531 743 538 315 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2834 2846 2866 2878 3280
Size: 32 135 32 1262 12 20 12 402
Cuts at: 3280 3312 5659 5691 5733 5765 1373
Size: 32 2347 32 42 32 2626
Fragments arranged by size:
2626 2347 1262 402 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4362 4608 669
Size: 988 281 2424 246 3079
Fragments arranged by size:
3079 2424 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3785 748
Size: 3037 3981
HpaI GTT'AAC
Cuts at: 4608 4608
Size: 7018
KpnI G_GTAC'C
Cuts at: 4343 4343
Size: 7018
MfeI C'AATT_G
Cuts at: 4617 4617
Size: 7018
MluI A'CGCG_T
Cuts at: 6894 6894
Size: 7018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 2868 3798 6132 6316 1305
Size: 1053 510 930 2334 184 2007
Fragments arranged by size:
2334 2007 1053 930 510 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2729 2915 3336 3743
Size: 54 1425 343 276 621 186 421 407
Cuts at: 3743 4205 10
Size: 462 2823
Fragments arranged by size:
2823 1425 621 462 421 407 343 276
186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 2751 3725 4976 5335 5494 518
Size: 648 1563 22 974 1251 359 159 2042
Fragments arranged by size:
2042 1563 1251 974 648 359 159 22
MspA1I CmG'CkG
Cuts at: 1606 2168 2285 2371 2520 2563 2639 3010 3124
Size: 562 117 86 149 43 76 371 114
Cuts at: 3124 3325 3908 4012 4434 5111 6259 6504 1606
Size: 201 583 104 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 562 422 371 245
201 149 117 114 104 86 76 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2209 513
Size: 552 939 205 5322
Fragments arranged by size:
5322 939 552 205
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7018
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7018
NotI GC'GGCC_GC
Cuts at: 4465 4465
Size: 7018
NspI r_CATG'y
Cuts at: 1132 1229 4376 5921 1132
Size: 97 3147 1545 2229
Fragments arranged by size:
3147 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2141 2251 2768 1348
Size: 793 110 517 5598
Fragments arranged by size:
5598 793 517 110
PciI A'CATG_T
Cuts at: 5917 5917
Size: 7018
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2298 2591 4147 1108
Size: 1190 293 1556 3979
Fragments arranged by size:
3979 1556 1190 293
PfoI T'CCnGG_A
Cuts at: 2336 3489 3510 3848 2336
Size: 1153 21 338 5506
Fragments arranged by size:
5506 1153 338 21
PmeI GTTT'AAAC
Cuts at: 4322 4322
Size: 7018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5087 5119 6600 6632 5087
Size: 32 1481 32 5473
Fragments arranged by size:
5473 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2362 3154 2191
Size: 171 792 6055
Fragments arranged by size:
6055 792 171
PshAI GACnn'nnGTC
Cuts at: 1613 2064 1613
Size: 451 6567
PsiI TTA'TAA
Cuts at: 4588 4588
Size: 7018
PspOMI G'GGCC_C
Cuts at: 2565 2764 2565
Size: 199 6819
PspXI vC'TCGA_Gb
Cuts at: 2975 2975
Size: 7018
PstI C_TGCA'G
Cuts at: 830 2032 2138 3666 4370 830
Size: 1202 106 1528 704 3478
Fragments arranged by size:
3478 1528 1202 704 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7018
PvuII CAG'CTG
Cuts at: 2371 2520 2639 3010 3325 4012 2371
Size: 149 119 371 315 687 5377
Fragments arranged by size:
5377 687 371 315 149 119
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 7018
SalI G'TCGA_C
Cuts at: 1936 4360 1936
Size: 2424 4594
SbfI CC_TGCA'GG
Cuts at: 4370 4370
Size: 7018
ScaI AGT'ACT
Cuts at: 5266 6978 5266
Size: 1712 5306
SexAI A'CCwGG_T
Cuts at: 3867 3867
Size: 7018
SfcI C'TryA_G
Cuts at: 826 1045 2028 2134 3662 3760 4366 5501 6182
Size: 219 983 106 1528 98 606 1135 681
Cuts at: 6182 6373 826
Size: 191 1471
Fragments arranged by size:
1528 1471 1135 983 681 606 219 191
106 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 2763 3749 4202 2763
Size: 986 453 5579
Fragments arranged by size:
5579 986 453
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7018
SmaI CCC'GGG
Cuts at: 1872 2389 4345 1872
Size: 517 1956 4545
Fragments arranged by size:
4545 1956 517
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 3788 5122 6023 6285 820
Size: 197 926 1032 813 1334 901 262 1553
Fragments arranged by size:
1553 1334 1032 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 4809 493
Size: 4316 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7018
SphI G_CATG'C
Cuts at: 1132 4376 1132
Size: 3244 3774
SspI AAT'ATT
Cuts at: 5 52 4942 5
Size: 47 4890 2081
Fragments arranged by size:
4890 2081 47
StuI AGG'CCT
Cuts at: 1517 3116 3682 1517
Size: 1599 566 4853
Fragments arranged by size:
4853 1599 566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2209 2221 4068 4457 513
Size: 552 939 205 12 1847 389 3074
Fragments arranged by size:
3074 1847 939 552 389 205 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 3575 4059 5045 5062 5215 5400
Size: 1255 897 1098 484 986 17 153 185
Cuts at: 5400 5845 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1098 986 897 484 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 3675 5264
Size: 274 80 33 51 1443 489 1209 1589
Cuts at: 5264 6976 96
Size: 1712 138
Fragments arranged by size:
1712 1589 1443 1209 489 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 2538 2570 3589 3621 6978 7010 2095
Size: 32 411 32 1019 32 3357 32 2103
Fragments arranged by size:
3357 2103 1019 411 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7018
XbaI T'CTAG_A
Cuts at: 4354 4354
Size: 7018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3329 3329
Size: 7018
XhoI C'TCGA_G
Cuts at: 1943 2975 1943
Size: 1032 5986
XmaI C'CCGG_G
Cuts at: 1870 2387 4343 1870
Size: 517 1956 4545
Fragments arranged by size:
4545 1956 517
XmnI GAAnn'nnTTC
Cuts at: 3116 3626 3926 5147 3116
Size: 510 300 1221 4987
Fragments arranged by size:
4987 1221 510 300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4826 276
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacII SanDI SapI SfoI SrfI SwaI