Restriction map for
pFN21AB8568
(Circular) (Six-base) MAPSORT of: pFN21AB8568.seq Check: 7541 from: 1 to: 7060
pFN21AB8568 7060 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4870 278
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2287 4403 1937
Size: 350 2116 4594
Fragments arranged by size:
4594 2116 350
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2796 3159 3468 3564 3600 4284 4299
Size: 644 520 363 309 96 36 684 15
Cuts at: 4299 5123 6507 6775 1632
Size: 824 1384 268 1917
Fragments arranged by size:
1917 1384 824 684 644 520 363 309
268 96 36 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6863
AflIII A'CryG_T
Cuts at: 1203 3317 5959 6936 1203
Size: 2114 2642 977 1327
Fragments arranged by size:
2642 2114 1327 977
AgeI A'CCGG_T
Cuts at: 5891 5891
Size: 7060
AhdI GACnn_n'nnGTC
Cuts at: 5789 5789
Size: 7060
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3804 1184
Size: 2620 4440
AlwNI CAG_nnn'CTG
Cuts at: 4803 6375 4803
Size: 1572 5488
ApaLI G'TGCA_C
Cuts at: 5117 6273 5117
Size: 1156 5904
ApoI r'AATT_y
Cuts at: 1493 2831 3065 3423 3838 4102 4200 4297 4369
Size: 1338 234 358 415 264 98 97 72
Cuts at: 4369 4595 4747 6856 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1338 415 358 264 234 226
152 98 97 72
AseI AT'TA_AT
Cuts at: 160 1032 4176 5614 160
Size: 872 3144 1438 1606
Fragments arranged by size:
3144 1606 1438 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 4385 1870
Size: 73 2442 4545
Fragments arranged by size:
4545 2442 73
BamHI G'GATC_C
Cuts at: 4390 4757 4390
Size: 367 6693
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4381 5836 618
Size: 325 217 627 2594 1455 1842
Fragments arranged by size:
2594 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4379 1391
Size: 2988 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 2784 3458 928
Size: 971 294 591 674 4530
Fragments arranged by size:
4530 971 674 591 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5251 5285 1903
Size: 34 50 34 3230 34 3678
Fragments arranged by size:
3678 3230 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4952 6162 4952
Size: 1210 5850
BclI T'GATC_A
Cuts at: 1548 2176 1548
Size: 628 6432
BfrBI ATG'CAT
Cuts at: 3754 3754
Size: 7060
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5671 136
Size: 107 122 71 631 605 3999 1525
Fragments arranged by size:
3999 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7055 7055
Size: 7060
BlpI GC'TnA_GC
Cuts at: 4477 4477
Size: 7060
Bme1580I G_kGCm'C
Cuts at: 1790 5121 6277 1790
Size: 3331 1156 2573
Fragments arranged by size:
3331 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4134 4791 5749
Size: 416 53 83 399 24 2717 657 958
Cuts at: 5749 442
Size: 1753
Fragments arranged by size:
2717 1753 958 657 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 2465 3183 3414 5720 1239
Size: 1107 119 718 231 2306 2579
Fragments arranged by size:
2579 2306 1107 718 231 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2115 5185 6050 6348 2115
Size: 3070 865 298 2827
Fragments arranged by size:
3070 2827 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5723 882
Size: 617 4224 2219
Fragments arranged by size:
4224 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4210 4851 493
Size: 711 3006 641 2702
Fragments arranged by size:
3006 2702 711 641
BsaBI GATnn'nnATC
Cuts at: 7054 7054
Size: 7060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4867 5249 5884 275
Size: 53 83 186 936 3334 382 635 1451
Fragments arranged by size:
3334 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5493 5891 6165 6312 6723 5493
Size: 398 274 147 411 5830
Fragments arranged by size:
5830 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4315 4345 1178
Size: 30 165 30 2912 30 3893
Fragments arranged by size:
3893 2912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3425 3905 1186
Size: 2239 480 4341
Fragments arranged by size:
4341 2239 480
BseYI C'CCAG_C
Cuts at: 3050 6263 6759 3050
Size: 3213 496 3351
Fragments arranged by size:
3351 3213 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6487
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4510 5271 6299 1540
Size: 275 186 2509 761 1028 2301
Fragments arranged by size:
2509 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3710 5121 5206 6277 3710
Size: 1411 85 1071 4493
Fragments arranged by size:
4493 1411 1071 85
BsmI GAATG_Cn'
Cuts at: 2770 3054 4571 4664 2770
Size: 284 1517 93 5166
Fragments arranged by size:
5166 1517 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3710 4379 5121 5206 6277 1391
Size: 399 1920 669 742 85 1071 2174
Fragments arranged by size:
2174 1920 1071 742 669 399 85
BspEI T'CCGG_A
Cuts at: 6723 6723
Size: 7060
BspHI T'CATG_A
Cuts at: 4949 6679 4949
Size: 1730 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4415 844
Size: 3571 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4947 1394
Size: 429 3124 3507
Fragments arranged by size:
3507 3124 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5555 5729 65
Size: 1183 4307 174 1396
Fragments arranged by size:
4307 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5704 5891 1700
Size: 110 3894 187 2869
Fragments arranged by size:
3894 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 5120 6132 5120
Size: 1012 6048
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2271 794
Size: 1477 5583
BstBI TT'CG_AA
Cuts at: 5864 5864
Size: 7060
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7060
BstYI r'GATC_y
Cuts at: 1972 4390 4757 5142 5159 6611 6697 6709 7055
Size: 2418 367 385 17 1452 86 12 346
Cuts at: 7055 1972
Size: 1977
Fragments arranged by size:
2418 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2288 2288
Size: 7060
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6321
Fragments arranged by size:
6321 683 56
BtsI GCAGTG_nn'
Cuts at: 3786 4576 5370 5390 3786
Size: 790 794 20 5456
Fragments arranged by size:
5456 794 790 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3888 3923 529
Size: 35 3324 35 3666
Fragments arranged by size:
3666 3324 35 35
DraI TTT'AAA
Cuts at: 2320 2785 4364 2320
Size: 465 1579 5016
Fragments arranged by size:
5016 1579 465
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6067 809
Size: 507 4751 1802
Fragments arranged by size:
4751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4507 5396 7025
Size: 54 1425 50 275 18 2677 889 1629
Cuts at: 7025 8
Size: 43
Fragments arranged by size:
2677 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2880 3135 3346 4275 4990 1353
Size: 863 664 255 211 929 715 3423
Fragments arranged by size:
3423 929 863 715 664 255 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5647 6021 6167 387
Size: 1064 138 4058 374 146 1280
Fragments arranged by size:
4058 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2346 2465 2796 3159 3183 3414
Size: 393 644 70 119 331 363 24 231
Cuts at: 3414 3468 3564 3600 4284 4299 5123 5720 6507
Size: 54 96 36 684 15 824 597 787
Cuts at: 6507 6775 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 684 644 597 393 363
331 268 231 119 96 70 54 36
24 15
EcoO109I rG'GnC_Cy
Cuts at: 4128 4128
Size: 7060
EcoRI G'AATT_C
Cuts at: 4297 4369 4747 4297
Size: 72 378 6610
Fragments arranged by size:
6610 378 72
EcoRV GAT'ATC
Cuts at: 2588 2588
Size: 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3413 3413
Size: 7060
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 6207 1678
Size: 4529 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3649 3681 5701 5733 5775
Size: 32 135 32 2077 32 2020 32 42
Cuts at: 5775 5807 1373
Size: 32 2626
Fragments arranged by size:
2626 2077 2020 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3850 4404 4650 669
Size: 988 281 1912 554 246 3079
Fragments arranged by size:
3079 1912 988 554 281 246
HindIII A'AGCT_T
Cuts at: 748 2148 4219 748
Size: 1400 2071 3589
Fragments arranged by size:
3589 2071 1400
HpaI GTT'AAC
Cuts at: 4650 4650
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 4659 4659
Size: 7060
MluI A'CGCG_T
Cuts at: 3317 6936 3317
Size: 3619 3441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6174 6358 1305
Size: 4869 184 2007
Fragments arranged by size:
4869 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5238
Fragments arranged by size:
5238 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2753 3374 5018 5377 5536 518
Size: 648 1587 621 1644 359 159 2042
Fragments arranged by size:
2042 1644 1587 648 621 359 159
MspA1I CmG'CkG
Cuts at: 1606 3304 4476 5153 6301 6546 1606
Size: 1698 1172 677 1148 245 2120
Fragments arranged by size:
2120 1698 1172 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6508
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NsiI A_TGCA'T
Cuts at: 3756 3756
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 2649 4418 5963 1132
Size: 97 1420 1769 1545 2229
Fragments arranged by size:
2229 1769 1545 1420 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7060
PciI A'CATG_T
Cuts at: 5959 5959
Size: 7060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 1108
Size: 1773 5287
PfoI T'CCnGG_A
Cuts at: 3199 3199
Size: 7060
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 6642 6674 5129
Size: 32 1481 32 5515
Fragments arranged by size:
5515 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4128 4128
Size: 7060
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7060
PsiI TTA'TAA
Cuts at: 3587 3921 4350 4630 3587
Size: 334 429 280 6017
Fragments arranged by size:
6017 429 334 280
PstI C_TGCA'G
Cuts at: 830 2194 4412 830
Size: 1364 2218 3478
Fragments arranged by size:
3478 2218 1364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
PvuII CAG'CTG
Cuts at: 3304 3304
Size: 7060
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SanDI GG'GwC_CC
Cuts at: 4128 4128
Size: 7060
SbfI CC_TGCA'GG
Cuts at: 4412 4412
Size: 7060
ScaI AGT'ACT
Cuts at: 5308 7020 5308
Size: 1712 5348
SfcI C'TryA_G
Cuts at: 826 1045 2190 2591 4408 5543 6224 6415 826
Size: 219 1145 401 1817 1135 681 191 1471
Fragments arranged by size:
1817 1471 1145 1135 681 401 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 4387 1872
Size: 2515 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2130 5164 6065 6327 820
Size: 197 926 187 3034 901 262 1553
Fragments arranged by size:
3034 1553 926 901 262 197 187
SnaBI TAC'GTA
Cuts at: 493 4210 4851 493
Size: 3717 641 2702
Fragments arranged by size:
3717 2702 641
SpeI A'CTAG_T
Cuts at: 152 4112 152
Size: 3960 3100
SphI G_CATG'C
Cuts at: 1132 4418 1132
Size: 3286 3774
SspI AAT'ATT
Cuts at: 5 52 2347 3515 4180 4984 5
Size: 47 2295 1168 665 804 2081
Fragments arranged by size:
2295 2081 1168 804 665 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7060
StyI C'CwwG_G
Cuts at: 513 1065 2537 2865 3097 4499 513
Size: 552 1472 328 232 1402 3074
Fragments arranged by size:
3074 1472 1402 552 328 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5087 5104 5257 5442 5887 325
Size: 1255 3507 17 153 185 445 1498
Fragments arranged by size:
3507 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3382 4079 5306
Size: 274 80 33 51 1443 1405 697 1227
Cuts at: 5306 7018 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1405 1227 697 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3828 3860 7020 7052 3828
Size: 32 3160 32 3836
Fragments arranged by size:
3836 3160 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7060
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 4385 1870
Size: 2515 4545
XmnI GAAnn'nnTTC
Cuts at: 3360 5189 3360
Size: 1829 5231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4868 276
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI