Restriction map for
pFN21AB8561
(Circular) (Six-base) MAPSORT of: pFN21AB8561.seq Check: 167 from: 1 to: 7606
pFN21AB8561 7606 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2903 4906 2903
Size: 2003 5603
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3828 5416 278
Size: 53 83 186 936 2292 1588 2468
Fragments arranged by size:
2468 2292 1588 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3333 4949 1937
Size: 1396 1616 4594
Fragments arranged by size:
4594 1616 1396
Acc65I G'GTAC_C
Cuts at: 2089 4927 2089
Size: 2838 4768
AclI AA'CG_TT
Cuts at: 1563 5733 6106 1563
Size: 4170 373 3063
Fragments arranged by size:
4170 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2306 2456 2948 3587 5669 7053 7321
Size: 450 224 150 492 639 2082 1384 268
Cuts at: 7321 1632
Size: 1917
Fragments arranged by size:
2082 1917 1384 639 492 450 268 224
150
AfeI AGC'GCT
Cuts at: 2498 2498
Size: 7606
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7409
AflIII A'CryG_T
Cuts at: 1203 6505 7482 1203
Size: 5302 977 1327
Fragments arranged by size:
5302 1327 977
AgeI A'CCGG_T
Cuts at: 3958 6437 3958
Size: 2479 5127
AhdI GACnn_n'nnGTC
Cuts at: 2596 6335 2596
Size: 3739 3867
AleI CACnn'nnGTG
Cuts at: 1166 3581 1166
Size: 2415 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 1184
Size: 1362 6244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 7574
AlwNI CAG_nnn'CTG
Cuts at: 3026 4264 4711 4807 5349 6921 3026
Size: 1238 447 96 542 1572 3711
Fragments arranged by size:
3711 1572 1238 542 447 96
ApaI G_GGCC'C
Cuts at: 2250 4443 2250
Size: 2193 5413
ApaLI G'TGCA_C
Cuts at: 3641 5663 6819 3641
Size: 2022 1156 4428
Fragments arranged by size:
4428 2022 1156
ApoI r'AATT_y
Cuts at: 1493 4915 5141 5293 7402 1493
Size: 3422 226 152 2109 1697
Fragments arranged by size:
3422 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6160 160
Size: 872 5128 1606
Fragments arranged by size:
5128 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
AvaI C'yCGr_G
Cuts at: 1870 1943 4456 4586 4931 1870
Size: 73 2513 130 345 4545
Fragments arranged by size:
4545 2513 345 130 73
AvrII C'CTAG_G
Cuts at: 3101 3101
Size: 7606
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2104 2137 2104
Size: 33 7573
BamHI G'GATC_C
Cuts at: 2859 4936 5303 2859
Size: 2077 367 5162
Fragments arranged by size:
5162 2077 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2931 2981 4700 4927
Size: 325 217 627 302 842 50 1719 227
Cuts at: 4927 6382 618
Size: 1455 1842
Fragments arranged by size:
1842 1719 1455 842 627 325 302 227
217 50
BanII G_rGCy'C
Cuts at: 1391 2025 2079 2250 2553 2613 3495 4125 4443
Size: 634 54 171 303 60 882 630 318
Cuts at: 4443 4560 4572 4737 4925 1391
Size: 117 12 165 188 4072
Fragments arranged by size:
4072 882 634 630 318 303 188 171
165 117 60 54 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 928
Size: 971 525 6110
Fragments arranged by size:
6110 971 525
BbvCI CC'TCA_GC
Cuts at: 2279 2279
Size: 7606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5797 5831 1903
Size: 34 3860 34 3678
Fragments arranged by size:
3860 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2132 3528 5498 6708 2132
Size: 1396 1970 1210 3030
Fragments arranged by size:
3030 1970 1396 1210
BclI T'GATC_A
Cuts at: 1548 3432 1548
Size: 1884 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2990 4459 4886
Size: 107 122 71 631 605 1318 1469 427
Cuts at: 4886 6217 136
Size: 1331 1525
Fragments arranged by size:
1525 1469 1331 1318 631 605 427 122
107 71
BglII A'GATC_T
Cuts at: 7601 7601
Size: 7606
BlpI GC'TnA_GC
Cuts at: 2231 5023 2231
Size: 2792 4814
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2250 2986 3645 4443 4703 5667 6823
Size: 258 202 736 659 798 260 964 1156
Cuts at: 6823 1790
Size: 2573
Fragments arranged by size:
2573 1156 964 798 736 659 260 258
202
BmgBI CAC'GTC
Cuts at: 1318 2116 1318
Size: 798 6808
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 5337 6295
Size: 416 53 83 399 24 2579 1341 958
Cuts at: 6295 442
Size: 1753
Fragments arranged by size:
2579 1753 1341 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4110 4110
Size: 7606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 2963 3176 3566 3644 3911 3938 4448
Size: 1328 396 213 390 78 267 27 510
Cuts at: 4448 4574 6266 1239
Size: 126 1692 2579
Fragments arranged by size:
2579 1692 1328 510 396 390 267 213
126 78 27
Bpu10I CC'TnA_GC
Cuts at: 2027 2279 2552 2726 3860 4343 2027
Size: 252 273 174 1134 483 5290
Fragments arranged by size:
5290 1134 483 273 252 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 4544 5731 6596 6894 2111
Size: 2433 1187 865 298 2823
Fragments arranged by size:
2823 2433 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2786 3921 6269 882
Size: 617 1287 1135 2348 2219
Fragments arranged by size:
2348 2219 1287 1135 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 4597 5397 493
Size: 711 1167 2226 800 2702
Fragments arranged by size:
2702 2226 1167 800 711
BsaBI GATnn'nnATC
Cuts at: 3476 7600 3476
Size: 4124 3482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3825 4403 4836 5413
Size: 53 83 186 936 2292 578 433 577
Cuts at: 5413 5795 6430 275
Size: 382 635 1451
Fragments arranged by size:
2292 1451 936 635 578 577 433 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2256 2478 2685 3660 3958 4500 6039 6437 6711
Size: 222 207 975 298 542 1539 398 274
Cuts at: 6711 6858 7269 2256
Size: 147 411 2593
Fragments arranged by size:
2593 1539 975 542 411 398 298 274
222 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 3545 3575 4343
Size: 30 165 30 1320 30 792 30 768
Cuts at: 4343 4373 1178
Size: 30 4411
Fragments arranged by size:
4411 1320 792 768 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2747 2750 2960 3503 4235 4367 4802 1186
Size: 1561 3 210 543 732 132 435 3990
Fragments arranged by size:
3990 1561 732 543 435 210 132 3
BseYI C'CCAG_C
Cuts at: 2536 2968 3532 3697 3987 4154 4238 4257 4462
Size: 432 564 165 290 167 84 19 205
Cuts at: 4462 6809 7305 2536
Size: 2347 496 2837
Fragments arranged by size:
2837 2347 564 496 432 290 205 167
165 84 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2351 2366 3376 4054 1163
Size: 573 567 48 15 1010 678 4715
Fragments arranged by size:
4715 1010 678 573 567 48 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5056 5817 6845 1540
Size: 275 186 3055 761 1028 2301
Fragments arranged by size:
3055 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2613 3078 3576 3645 4560 4572 4737 5667
Size: 60 465 498 69 915 12 165 930
Cuts at: 5667 5752 6823 2553
Size: 85 1071 3336
Fragments arranged by size:
3336 1071 930 915 498 465 165 85
69 60 12
BsmI GAATG_Cn'
Cuts at: 4096 5117 5210 4096
Size: 1021 93 6492
Fragments arranged by size:
6492 1021 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3767 3767
Size: 7606
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2048 2079 2250 2553 2613 2986
Size: 399 235 23 31 171 303 60 373
Cuts at: 2986 3078 3495 3576 3645 4125 4443 4560 4572
Size: 92 417 81 69 480 318 117 12
Cuts at: 4572 4703 4737 4925 5667 5752 6823 1391
Size: 131 34 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 417 399 373 318
303 235 188 171 131 117 92 85
81 69 60 34 31 23 12
BspEI T'CCGG_A
Cuts at: 2478 2685 3660 7269 2478
Size: 207 975 3609 2815
Fragments arranged by size:
3609 2815 975 207
BspHI T'CATG_A
Cuts at: 2745 3417 3435 3975 5495 7225 2745
Size: 672 18 540 1520 1730 3126
Fragments arranged by size:
3126 1730 1520 672 540 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 3386 4854 4906 4961 844
Size: 2059 483 1468 52 55 3489
Fragments arranged by size:
3489 2059 1468 483 55 52
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 3550 5493 1394
Size: 429 412 1315 1943 3507
Fragments arranged by size:
3507 1943 1315 429 412
BsrDI GCAATG_nn'
Cuts at: 65 1248 3261 3651 4068 6101 6275 65
Size: 1183 2013 390 417 2033 174 1396
Fragments arranged by size:
2033 2013 1396 1183 417 390 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 2768 3060 3930 3958 4008 6250
Size: 110 245 713 292 870 28 50 2242
Cuts at: 6250 6437 1700
Size: 187 2869
Fragments arranged by size:
2869 2242 870 713 292 245 187 110
50 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7606
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7606
BssSI C'ACGA_G
Cuts at: 3581 5666 6678 3581
Size: 2085 1012 4509
Fragments arranged by size:
4509 2085 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3636 794
Size: 2842 4764
BstBI TT'CG_AA
Cuts at: 6410 6410
Size: 7606
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7606
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2899 4117 4195 4876 2086
Size: 813 1218 78 681 4816
Fragments arranged by size:
4816 1218 813 681 78
BstYI r'GATC_y
Cuts at: 1972 2202 2307 2859 3138 3855 4936 5303 5688
Size: 230 105 552 279 717 1081 367 385
Cuts at: 5688 5705 7157 7243 7255 7601 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1081 717 552 385 367 346
279 230 105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 3612 4074 4371 513
Size: 552 1014 1533 462 297 3748
Fragments arranged by size:
3748 1533 1014 552 462 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6867
Fragments arranged by size:
6867 683 56
BtsI GCAGTG_nn'
Cuts at: 3435 3593 5122 5916 5936 3435
Size: 158 1529 794 20 5105
Fragments arranged by size:
5105 1529 794 158 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7571
DraI TTT'AAA
Cuts at: 4910 4910
Size: 7606
DraIII CAC_nnn'GTG
Cuts at: 3463 3463
Size: 7606
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3656 6613 809
Size: 507 2340 2957 1802
Fragments arranged by size:
2957 2340 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2901 4878 5053
Size: 54 1425 50 275 18 1071 1977 175
Cuts at: 5053 5942 7571 8
Size: 889 1629 43
Fragments arranged by size:
1977 1629 1425 1071 889 275 175 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5053 1537
Size: 275 3241 4090
Fragments arranged by size:
4090 3241 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3480 4245 5536 1353
Size: 2127 765 1291 3423
Fragments arranged by size:
3423 2127 1291 765
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2254 2852 6193 6567 6713 387
Size: 1064 138 665 598 3341 374 146 1280
Fragments arranged by size:
3341 1280 1064 665 598 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2611 4558 4570 4735 2551
Size: 60 1947 12 165 5422
Fragments arranged by size:
5422 1947 165 60 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2306 2456 2567 2948 2963 3176
Size: 393 450 224 150 111 381 15 213
Cuts at: 3176 3566 3587 3644 3911 3938 4448 4574 5669
Size: 390 21 57 267 27 510 126 1095
Cuts at: 5669 6266 7053 7321 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1095 787 597 510 450 393 390
381 268 267 224 213 150 126 111
57 27 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2618 2725 3020 4784 2438
Size: 180 107 295 1764 5260
Fragments arranged by size:
5260 1764 295 180 107
EcoO109I rG'GnC_Cy
Cuts at: 2066 4439 4440 2066
Size: 2373 1 5232
Fragments arranged by size:
5232 2373 1
EcoRI G'AATT_C
Cuts at: 4915 5293 4915
Size: 378 7228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2620 3895 2152
Size: 468 1275 5863
Fragments arranged by size:
5863 1275 468
FspI TGC'GCA
Cuts at: 6112 6112
Size: 7606
HaeII r_GCGC'y
Cuts at: 1678 2500 6753 1678
Size: 822 4253 2531
Fragments arranged by size:
4253 2531 822
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 4343 4375 6247
Size: 32 135 32 1010 32 1729 32 1872
Cuts at: 6247 6279 6321 6353 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1872 1729 1010 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4950 5196 669
Size: 988 281 3012 246 3079
Fragments arranged by size:
3079 3012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7606
HpaI GTT'AAC
Cuts at: 5196 5196
Size: 7606
KpnI G_GTAC'C
Cuts at: 2093 4931 2093
Size: 2838 4768
MfeI C'AATT_G
Cuts at: 5205 5205
Size: 7606
MluI A'CGCG_T
Cuts at: 7482 7482
Size: 7606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 3826 4258 6720 6904 1305
Size: 1320 1201 432 2462 184 2007
Fragments arranged by size:
2462 2007 1320 1201 432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4880 10
Size: 54 1425 343 3048 2736
Fragments arranged by size:
3048 2736 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2831 3581 3671 3911 4073 5564
Size: 648 918 747 750 90 240 162 1491
Cuts at: 5564 5923 6082 518
Size: 359 159 2042
Fragments arranged by size:
2042 1491 918 750 747 648 359 240
162 159 90
MspA1I CmG'CkG
Cuts at: 1606 2536 2802 2869 2941 2968 3984 4264 4579
Size: 930 266 67 72 27 1016 280 315
Cuts at: 4579 5022 5699 6847 7092 1606
Size: 443 677 1148 245 2120
Fragments arranged by size:
2120 1148 1016 930 677 443 315 280
266 245 72 67 27
NaeI GCC'GGC
Cuts at: 2057 2770 3062 2057
Size: 713 292 6601
Fragments arranged by size:
6601 713 292
NcoI C'CATG_G
Cuts at: 513 1065 2079 3612 4074 4371 513
Size: 552 1014 1533 462 297 3748
Fragments arranged by size:
3748 1533 1014 552 462 297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7606
NgoMIV G'CCGG_C
Cuts at: 2055 2768 3060 2055
Size: 713 292 6601
Fragments arranged by size:
6601 713 292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7606
NotI GC'GGCC_GC
Cuts at: 5053 5053
Size: 7606
NspI r_CATG'y
Cuts at: 1132 1229 2494 3850 4964 6509 1132
Size: 97 1265 1356 1114 1545 2229
Fragments arranged by size:
2229 1545 1356 1265 1114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7606
PciI A'CATG_T
Cuts at: 6505 6505
Size: 7606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3257 3544 3774 1108
Size: 2149 287 230 4940
Fragments arranged by size:
4940 2149 287 230
PfoI T'CCnGG_A
Cuts at: 2205 4134 4215 4299 2205
Size: 1929 81 84 5512
Fragments arranged by size:
5512 1929 84 81
PmeI GTTT'AAAC
Cuts at: 4910 4910
Size: 7606
PmlI CAC'GTG
Cuts at: 2371 2371
Size: 7606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5675 5707 7188 7220 3545
Size: 32 2098 32 1481 32 3931
Fragments arranged by size:
3931 2098 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3708 1613
Size: 2095 5511
PsiI TTA'TAA
Cuts at: 5176 5176
Size: 7606
PspOMI G'GGCC_C
Cuts at: 2246 4439 2246
Size: 2193 5413
PstI C_TGCA'G
Cuts at: 830 2074 2535 3069 3191 3600 4212 4903 4958
Size: 1244 461 534 122 409 612 691 55
Cuts at: 4958 830
Size: 3478
Fragments arranged by size:
3478 1244 691 612 534 461 409 122
55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7606
PvuII CAG'CTG
Cuts at: 2536 2869 2968 4264 4579 2536
Size: 333 99 1296 315 5563
Fragments arranged by size:
5563 1296 333 315 99
SacI G_AGCT'C
Cuts at: 2553 2613 4560 4572 4737 2553
Size: 60 1947 12 165 5422
Fragments arranged by size:
5422 1947 165 60 12
SalI G'TCGA_C
Cuts at: 1936 4948 1936
Size: 3012 4594
SbfI CC_TGCA'GG
Cuts at: 4958 4958
Size: 7606
ScaI AGT'ACT
Cuts at: 2961 5854 7566 2961
Size: 2893 1712 3001
Fragments arranged by size:
3001 2893 1712
SexAI A'CCwGG_T
Cuts at: 2838 4512 2838
Size: 1674 5932
SfcI C'TryA_G
Cuts at: 826 1045 2070 2140 2531 3065 3187 3596 4208
Size: 219 1025 70 391 534 122 409 612
Cuts at: 4208 4899 4954 6089 6770 6961 826
Size: 691 55 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 691 681 612 534 409
391 219 191 122 70 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 4886 4886
Size: 7606
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7606
SmaI CCC'GGG
Cuts at: 1872 4933 1872
Size: 3061 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 4559 5710 6611 6873 820
Size: 197 926 183 2433 1151 901 262 1553
Fragments arranged by size:
2433 1553 1151 926 901 262 197 183
SnaBI TAC'GTA
Cuts at: 493 5397 493
Size: 4904 2702
SpeI A'CTAG_T
Cuts at: 152 2873 152
Size: 2721 4885
SphI G_CATG'C
Cuts at: 1132 4964 1132
Size: 3832 3774
SspI AAT'ATT
Cuts at: 5 52 5530 5
Size: 47 5478 2081
Fragments arranged by size:
5478 2081 47
StuI AGG'CCT
Cuts at: 1517 2993 1517
Size: 1476 6130
StyI C'CwwG_G
Cuts at: 513 1065 2079 3079 3101 3612 4074 4371 4869
Size: 552 1014 1000 22 511 462 297 498
Cuts at: 4869 5045 513
Size: 176 3074
Fragments arranged by size:
3074 1014 1000 552 511 498 462 297
176 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 2337 4162 4718 4908 5633 5650
Size: 1255 747 10 1825 556 190 725 17
Cuts at: 5650 5803 5988 6433 325
Size: 153 185 445 1498
Fragments arranged by size:
1825 1498 1255 747 725 556 445 190
185 153 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2959 3588 4167
Size: 274 80 33 51 1443 982 629 579
Cuts at: 4167 5852 7564 96
Size: 1685 1712 138
Fragments arranged by size:
1712 1685 1443 982 629 579 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 2366 2398 2723 2755 3097 3129 4322
Size: 32 52 32 325 32 342 32 1193
Cuts at: 4322 4354 4461 4493 7566 7598 2282
Size: 32 107 32 3073 32 2290
Fragments arranged by size:
3073 2290 1193 342 325 107 52 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2840 1316
Size: 1524 6082
XbaI T'CTAG_A
Cuts at: 4942 4942
Size: 7606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 3151 3619 4492 2257
Size: 894 468 873 5371
Fragments arranged by size:
5371 894 873 468
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7606
XmaI C'CCGG_G
Cuts at: 1870 4931 1870
Size: 3061 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 2753 2811 5735 2168
Size: 585 58 2924 4039
Fragments arranged by size:
4039 2924 585 58
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3826 5414 276
Size: 53 83 186 936 2292 1588 2468
Fragments arranged by size:
2468 2292 1588 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI