Restriction map for
pFN21AB8560
(Circular) (Six-base) MAPSORT of: pFN21AB8560.seq Check: 964 from: 1 to: 7435
pFN21AB8560 7435 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2610 2610
Size: 7435
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5245 278
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4383 4778 1937
Size: 2446 395 4594
Fragments arranged by size:
4594 2446 395
Acc65I G'GTAC_C
Cuts at: 4756 4756
Size: 7435
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2593 4637 5498 6882 7150 1632
Size: 961 2044 861 1384 268 1917
Fragments arranged by size:
2044 1917 1384 961 861 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7238
AflIII A'CryG_T
Cuts at: 1203 6334 7311 1203
Size: 5131 977 1327
Fragments arranged by size:
5131 1327 977
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 6164 6164
Size: 7435
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4494 4526 4494
Size: 32 7403
AlwNI CAG_nnn'CTG
Cuts at: 3714 4241 5178 6750 3714
Size: 527 937 1572 4399
Fragments arranged by size:
4399 1572 937 527
ApaLI G'TGCA_C
Cuts at: 5492 6648 5492
Size: 1156 6279
ApoI r'AATT_y
Cuts at: 1493 2041 2060 2476 2516 2785 3122 3191 3275
Size: 548 19 416 40 269 337 69 84
Cuts at: 3275 3410 3694 4435 4535 4744 4970 5122 7231
Size: 135 284 741 100 209 226 152 2109
Cuts at: 7231 1493
Size: 1697
Fragments arranged by size:
2109 1697 741 548 416 337 284 269
226 209 152 135 100 84 69 40
19
AseI AT'TA_AT
Cuts at: 160 1032 2151 3422 5989 160
Size: 872 1119 1271 2567 1606
Fragments arranged by size:
2567 1606 1271 1119 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 4760 1870
Size: 73 2817 4545
Fragments arranged by size:
4545 2817 73
BamHI G'GATC_C
Cuts at: 4765 5132 4765
Size: 367 7068
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4756 6211 618
Size: 325 217 627 2969 1455 1842
Fragments arranged by size:
2969 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3766 4754 1391
Size: 2375 988 4072
Fragments arranged by size:
4072 2375 988
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3005 3080 928
Size: 971 1106 75 5283
Fragments arranged by size:
5283 1106 971 75
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5626 5660 1903
Size: 34 3689 34 3678
Fragments arranged by size:
3689 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4012 5327 6537 4012
Size: 1315 1210 4910
Fragments arranged by size:
4910 1315 1210
BclI T'GATC_A
Cuts at: 1548 3169 1548
Size: 1621 5814
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6046 136
Size: 107 122 71 631 605 4374 1525
Fragments arranged by size:
4374 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4335 7430 4335
Size: 3095 4340
BlpI GC'TnA_GC
Cuts at: 4852 4852
Size: 7435
Bme1580I G_kGCm'C
Cuts at: 1790 5496 6652 1790
Size: 3706 1156 2573
Fragments arranged by size:
3706 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5166 6124 442
Size: 416 53 83 399 24 3749 958 1753
Fragments arranged by size:
3749 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2583 4659 6095 1239
Size: 816 528 2076 1436 2579
Fragments arranged by size:
2579 2076 1436 816 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4704 5560 6425 6723 4704
Size: 856 865 298 5416
Fragments arranged by size:
5416 865 856 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6098 882
Size: 617 4599 2219
Fragments arranged by size:
4599 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5226 493
Size: 711 4022 2702
Fragments arranged by size:
4022 2702 711
BsaBI GATnn'nnATC
Cuts at: 2814 4230 7429 2814
Size: 1416 3199 2820
Fragments arranged by size:
3199 2820 1416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5242 5624 6259 275
Size: 53 83 186 936 3709 382 635 1451
Fragments arranged by size:
3709 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5868 6266 6540 6687 7098 5868
Size: 398 274 147 411 6205
Fragments arranged by size:
6205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3370 3400 4261 4291 4494
Size: 30 165 30 1967 30 861 30 203
Cuts at: 4494 4524 1178
Size: 30 4089
Fragments arranged by size:
4089 1967 861 203 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2652 1186
Size: 1466 5969
BseYI C'CCAG_C
Cuts at: 4611 6638 7134 4611
Size: 2027 496 4912
Fragments arranged by size:
4912 2027 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6862
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3664 3766 5496 5581 6652 3664
Size: 102 1730 85 1071 4447
Fragments arranged by size:
4447 1730 1071 102 85
BsmI GAATG_Cn'
Cuts at: 2659 4946 5039 2659
Size: 2287 93 5055
Fragments arranged by size:
5055 2287 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2002 2002
Size: 7435
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3664 3766 4754 5496 5581 6652 1391
Size: 399 1874 102 988 742 85 1071 2174
Fragments arranged by size:
2174 1874 1071 988 742 399 102 85
BspEI T'CCGG_A
Cuts at: 7098 7098
Size: 7435
BspHI T'CATG_A
Cuts at: 4474 5324 7054 4474
Size: 850 1730 4855
Fragments arranged by size:
4855 1730 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2610 4790 844
Size: 1766 2180 3489
Fragments arranged by size:
3489 2180 1766
BsrBI CCG'CTC
Cuts at: 1394 1823 5322 1394
Size: 429 3499 3507
Fragments arranged by size:
3507 3499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3002 3014 4260 4628 5930 6104 65
Size: 1183 1754 12 1246 368 1302 174 1396
Fragments arranged by size:
1754 1396 1302 1246 1183 368 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 6079 6266 1700
Size: 110 4269 187 2869
Fragments arranged by size:
4269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4291 4496 96
Size: 4195 205 3035
Fragments arranged by size:
4195 3035 205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 5495 6507 5495
Size: 1012 6423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7435
BstBI TT'CG_AA
Cuts at: 6239 6239
Size: 7435
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7435
BstYI r'GATC_y
Cuts at: 1972 2293 4199 4335 4660 4765 5132 5517 5534
Size: 321 1906 136 325 105 367 385 17
Cuts at: 5534 6986 7072 7084 7430 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1906 1452 385 367 346 325 321
136 105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6696
Fragments arranged by size:
6696 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 2340 2620 3041 4951 5745 5765 2257
Size: 83 280 421 1910 794 20 3927
Fragments arranged by size:
3927 1910 794 421 280 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7400
DraI TTT'AAA
Cuts at: 2199 4650 4739 2199
Size: 2451 89 4895
Fragments arranged by size:
4895 2451 89
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6442 809
Size: 507 5126 1802
Fragments arranged by size:
5126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4882 5771 7400
Size: 54 1425 50 275 18 3052 889 1629
Cuts at: 7400 8
Size: 43
Fragments arranged by size:
3052 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2346 2494 2535 3541 4141 5365 1353
Size: 741 252 148 41 1006 600 1224 3423
Fragments arranged by size:
3423 1224 1006 741 600 252 148 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6022 6396 6542 387
Size: 1064 138 4433 374 146 1280
Fragments arranged by size:
4433 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3764 3764
Size: 7435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2583 2593 4637 4659 5498 6095
Size: 393 423 528 10 2044 22 839 597
Cuts at: 6095 6882 7150 1239
Size: 787 268 1524
Fragments arranged by size:
2044 1524 839 787 597 528 423 393
268 22 10
EcoO109I rG'GnC_Cy
Cuts at: 3044 3044
Size: 7435
EcoRI G'AATT_C
Cuts at: 2041 3191 4744 5122 2041
Size: 1150 1553 378 4354
Fragments arranged by size:
4354 1553 1150 378
FspI TGC'GCA
Cuts at: 5941 5941
Size: 7435
HaeII r_GCGC'y
Cuts at: 1678 6582 1678
Size: 4904 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3567 3599 4173 4205 4261
Size: 32 135 32 1995 32 574 32 56
Cuts at: 4261 4293 6076 6108 6150 6182 1373
Size: 32 1783 32 42 32 2626
Fragments arranged by size:
2626 1995 1783 574 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4130 4384 4779 5025 669
Size: 988 281 2192 254 395 246 3079
Fragments arranged by size:
3079 2192 988 395 281 254 246
HindIII A'AGCT_T
Cuts at: 748 3097 748
Size: 2349 5086
HpaI GTT'AAC
Cuts at: 5025 5025
Size: 7435
KpnI G_GTAC'C
Cuts at: 4760 4760
Size: 7435
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3711 4029 4509 6549 6733 1305
Size: 2406 318 480 2040 184 2007
Fragments arranged by size:
2406 2040 2007 480 318 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5613
Fragments arranged by size:
5613 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3007 4056 4518 5393 5752 5911 518
Size: 648 1841 1049 462 875 359 159 2042
Fragments arranged by size:
2042 1841 1049 875 648 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 3554 4615 4851 5528 6676 6921 1606
Size: 1948 1061 236 677 1148 245 2120
Fragments arranged by size:
2120 1948 1148 1061 677 245 236
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6883
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7435
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7435
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NspI r_CATG'y
Cuts at: 1132 1229 4793 6338 1132
Size: 97 3564 1545 2229
Fragments arranged by size:
3564 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7435
PciI A'CATG_T
Cuts at: 6334 6334
Size: 7435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7435
PfoI T'CCnGG_A
Cuts at: 2071 3439 2071
Size: 1368 6067
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4494 4526 5504 5536 7017 7049 4494
Size: 32 978 32 1481 32 4880
Fragments arranged by size:
4880 1481 978 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3044 3044
Size: 7435
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7435
PsiI TTA'TAA
Cuts at: 2514 3608 5005 2514
Size: 1094 1397 4944
Fragments arranged by size:
4944 1397 1094
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2704 2736 2704
Size: 32 7403
PstI C_TGCA'G
Cuts at: 830 2020 3036 4787 830
Size: 1190 1016 1751 3478
Fragments arranged by size:
3478 1751 1190 1016
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
PvuII CAG'CTG
Cuts at: 3554 4615 3554
Size: 1061 6374
SacI G_AGCT'C
Cuts at: 3766 3766
Size: 7435
SalI G'TCGA_C
Cuts at: 1936 4382 4777 1936
Size: 2446 395 4594
Fragments arranged by size:
4594 2446 395
SanDI GG'GwC_CC
Cuts at: 3044 3044
Size: 7435
SapI GCTCTTCn'nnn_
Cuts at: 3541 3541
Size: 7435
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 2221 2647 5683 7395 2221
Size: 426 3036 1712 2261
Fragments arranged by size:
3036 2261 1712 426
SfcI C'TryA_G
Cuts at: 826 1045 2016 3032 3336 3665 3935 4314 4783
Size: 219 971 1016 304 329 270 379 469
Cuts at: 4783 5918 6599 6790 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1016 971 681 469 379 329
304 270 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 4762 1872
Size: 2890 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4719 5539 6440 6702 820
Size: 197 926 2776 820 901 262 1553
Fragments arranged by size:
2776 1553 926 901 820 262 197
SnaBI TAC'GTA
Cuts at: 493 5226 493
Size: 4733 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7435
SphI G_CATG'C
Cuts at: 1132 4793 1132
Size: 3661 3774
SspI AAT'ATT
Cuts at: 5 52 2261 2378 3447 5359 5
Size: 47 2209 117 1069 1912 2081
Fragments arranged by size:
2209 2081 1912 1069 117 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7435
StyI C'CwwG_G
Cuts at: 513 1065 2340 4874 513
Size: 552 1275 2534 3074
Fragments arranged by size:
3074 2534 1275 552
SwaI ATTT'AAAT
Cuts at: 4650 4650
Size: 7435
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 5462 5479 5632 5817 6262 325
Size: 1255 996 2886 17 153 185 445 1498
Fragments arranged by size:
2886 1498 1255 996 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2219 2645 4291
Size: 274 80 33 51 1443 242 426 1646
Cuts at: 4291 4496 5681 7393 96
Size: 205 1185 1712 138
Fragments arranged by size:
1712 1646 1443 1185 426 274 242 205
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3087 3119 7395 7427 3087
Size: 32 4276 32 3095
Fragments arranged by size:
4276 3095 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7435
XbaI T'CTAG_A
Cuts at: 4259 4547 4771 4259
Size: 288 224 6923
Fragments arranged by size:
6923 288 224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7435
XmaI C'CCGG_G
Cuts at: 1870 4760 1870
Size: 2890 4545
XmnI GAAnn'nnTTC
Cuts at: 2045 2684 2744 2843 4407 4509 5564 2045
Size: 639 60 99 1564 102 1055 3916
Fragments arranged by size:
3916 1564 1055 639 102 99 60
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5243 276
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII
SexAI SfiI SfoI SrfI XcmI