Restriction map for
pFN21AB8559
(Circular) (Six-base) MAPSORT of: pFN21AB8559.seq Check: 5554 from: 1 to: 7723
pFN21AB8559 7723 bp
With 182 enzymes: SgfI *
March 28, 2012 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2453 4058 2453
Size: 1605 6118
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5533 278
Size: 53 83 186 936 3997 2468
Fragments arranged by size:
3997 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5066 1937
Size: 3129 4594
Acc65I G'GTAC_C
Cuts at: 4232 5044 4232
Size: 812 6911
AclI AA'CG_TT
Cuts at: 1563 5850 6223 1563
Size: 4287 373 3063
Fragments arranged by size:
4287 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 3281 3708 4934 5786 7170 7438 1632
Size: 1470 179 427 1226 852 1384 268 1917
Fragments arranged by size:
1917 1470 1384 1226 852 427 268 179
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7526
AflIII A'CryG_T
Cuts at: 1203 3430 4212 4540 4653 6622 7599 1203
Size: 2227 782 328 113 1969 977 1327
Fragments arranged by size:
2227 1969 1327 977 782 328 113
AgeI A'CCGG_T
Cuts at: 6554 6554
Size: 7723
AhdI GACnn_n'nnGTC
Cuts at: 6452 6452
Size: 7723
AleI CACnn'nnGTG
Cuts at: 1166 4656 1166
Size: 3490 4233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3828 1184
Size: 2644 5079
AlwNI CAG_nnn'CTG
Cuts at: 5466 7038 5466
Size: 1572 6151
ApaLI G'TGCA_C
Cuts at: 2329 5780 6936 2329
Size: 3451 1156 3116
Fragments arranged by size:
3451 3116 1156
ApoI r'AATT_y
Cuts at: 1493 5032 5258 5410 7519 1493
Size: 3539 226 152 2109 1697
Fragments arranged by size:
3539 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6277 160
Size: 872 5245 1606
Fragments arranged by size:
5245 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2138 5048 1870
Size: 73 109 86 2910 4545
Fragments arranged by size:
4545 2910 109 86 73
BamHI G'GATC_C
Cuts at: 5053 5420 5053
Size: 367 7356
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2465 2990 4070 4232
Size: 325 217 627 327 351 525 1080 162
Cuts at: 4232 5044 6499 618
Size: 812 1455 1842
Fragments arranged by size:
1842 1455 1080 812 627 525 351 327
325 217 162
BanII G_rGCy'C
Cuts at: 1391 2138 5042 1391
Size: 747 2904 4072
Fragments arranged by size:
4072 2904 747
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3650 928
Size: 971 1751 5001
Fragments arranged by size:
5001 1751 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5914 5948 1903
Size: 34 3977 34 3678
Fragments arranged by size:
3977 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2907 4096 5615 6825 2907
Size: 1189 1519 1210 3805
Fragments arranged by size:
3805 1519 1210 1189
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7723
BfrBI ATG'CAT
Cuts at: 3233 3233
Size: 7723
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 6334 136
Size: 107 122 71 631 605 440 4222 1525
Fragments arranged by size:
4222 1525 631 605 440 122 107 71
BglII A'GATC_T
Cuts at: 2767 7718 2767
Size: 4951 2772
BlpI GC'TnA_GC
Cuts at: 5140 5140
Size: 7723
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2333 4895 5784 6940 1790
Size: 327 216 2562 889 1156 2573
Fragments arranged by size:
2573 2562 1156 889 327 216
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 3825 5454
Size: 416 53 83 399 24 2249 159 1629
Cuts at: 5454 6412 442
Size: 958 1753
Fragments arranged by size:
2249 1753 1629 958 416 399 159 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4506 4506
Size: 7723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2575 3662 3780 6383 1239
Size: 1336 1087 118 2603 2579
Fragments arranged by size:
2603 2579 1336 1087 118
Bpu10I CC'TnA_GC
Cuts at: 2973 4301 2973
Size: 1328 6395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4768 5848 6713 7011 4768
Size: 1080 865 298 5480
Fragments arranged by size:
5480 1080 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4956 6386 882
Size: 617 3457 1430 2219
Fragments arranged by size:
3457 2219 1430 617
BsaAI yAC'GTr
Cuts at: 493 1204 3937 4541 5514 493
Size: 711 2733 604 973 2702
Fragments arranged by size:
2733 2702 973 711 604
BsaBI GATnn'nnATC
Cuts at: 2355 2874 4353 7717 2355
Size: 519 1479 3364 2361
Fragments arranged by size:
3364 2361 1479 519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5530 5912 6547 275
Size: 53 83 186 936 3997 382 635 1451
Fragments arranged by size:
3997 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2121 6156 6554 6828 6975 7386 2121
Size: 4035 398 274 147 411 2458
Fragments arranged by size:
4035 2458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 3753 3783 4140
Size: 30 165 30 771 30 1549 30 357
Cuts at: 4140 4170 1178
Size: 30 4731
Fragments arranged by size:
4731 1549 771 357 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2496 1186
Size: 970 340 6413
Fragments arranged by size:
6413 970 340
BseYI C'CCAG_C
Cuts at: 6926 7422 6926
Size: 496 7227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2325 3000 3798 4086 4278 4718 1163
Size: 573 589 675 798 288 192 440 4168
Fragments arranged by size:
4168 798 675 589 573 440 288 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5173 5934 6962 1540
Size: 275 186 3172 761 1028 2301
Fragments arranged by size:
3172 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 4472 5784 5869 6940 2333
Size: 2139 1312 85 1071 3116
Fragments arranged by size:
3116 2139 1312 1071 85
BsmI GAATG_Cn'
Cuts at: 2289 3580 4305 5234 5327 2289
Size: 1291 725 929 93 4685
Fragments arranged by size:
4685 1291 929 725 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2138 2333 4472 4895 5042 5784
Size: 399 327 21 195 2139 423 147 742
Cuts at: 5784 5869 6940 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2139 1071 742 423 399 327 195
147 85 21
BspEI T'CCGG_A
Cuts at: 2121 7386 2121
Size: 5265 2458
BspHI T'CATG_A
Cuts at: 2878 3706 5612 7342 2878
Size: 828 1906 1730 3259
Fragments arranged by size:
3259 1906 1730 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 2453 4058 5078 844
Size: 1429 180 1605 1020 3489
Fragments arranged by size:
3489 1605 1429 1020 180
BsrBI CCG'CTC
Cuts at: 1394 1823 5610 1394
Size: 429 3787 3507
Fragments arranged by size:
3787 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3235 3392 3843 6218 6392 65
Size: 1183 1987 157 451 2375 174 1396
Fragments arranged by size:
2375 1987 1396 1183 451 174 157
BsrFI r'CCGG_y
Cuts at: 1700 1810 2231 6367 6554 1700
Size: 110 421 4136 187 2869
Fragments arranged by size:
4136 2869 421 187 110
BsrGI T'GTAC_A
Cuts at: 96 2312 2785 3019 96
Size: 2216 473 234 4800
Fragments arranged by size:
4800 2216 473 234
BssHII G'CGCG_C
Cuts at: 1925 2223 1925
Size: 298 7425
BssSI C'ACGA_G
Cuts at: 5783 6795 5783
Size: 1012 6711
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4268 4852 794
Size: 3474 584 3665
Fragments arranged by size:
3665 3474 584
BstBI TT'CG_AA
Cuts at: 4309 6527 4309
Size: 2218 5505
BstEII G'GTnAC_C
Cuts at: 1184 2750 3290 1184
Size: 1566 540 5617
Fragments arranged by size:
5617 1566 540
BstXI CCAn_nnnn'nTGG
Cuts at: 3287 3870 3287
Size: 583 7140
BstYI r'GATC_y
Cuts at: 1972 2767 5053 5420 5805 5822 7274 7360 7372
Size: 795 2286 367 385 17 1452 86 12
Cuts at: 7372 7718 1972
Size: 346 1977
Fragments arranged by size:
2286 1977 1452 795 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3869 513
Size: 552 2804 4367
Fragments arranged by size:
4367 2804 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2354 3101 487
Size: 683 56 1128 747 5109
Fragments arranged by size:
5109 1128 747 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 2692 4706 5239 6033 6053 2501
Size: 191 2014 533 794 20 4171
Fragments arranged by size:
4171 2014 794 533 191 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7688
DraI TTT'AAA
Cuts at: 3147 5027 3147
Size: 1880 5843
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 7723
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6730 809
Size: 507 5414 1802
Fragments arranged by size:
5414 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3112 3394 4648
Size: 54 1425 50 275 18 1282 282 1254
Cuts at: 4648 5170 6059 7688 8
Size: 522 889 1629 43
Fragments arranged by size:
1629 1425 1282 1254 889 522 282 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5170 1537
Size: 275 3358 4090
Fragments arranged by size:
4090 3358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3708 4247 4449 4475 4717 5653 1353
Size: 2355 539 202 26 242 936 3423
Fragments arranged by size:
3423 2355 936 539 242 202 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6310 6684 6830 387
Size: 1064 138 4721 374 146 1280
Fragments arranged by size:
4721 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2575 3102 3281 3662 3708 3780 4934
Size: 393 943 527 179 381 46 72 1154
Cuts at: 4934 5786 6383 7170 7438 1239
Size: 852 597 787 268 1524
Fragments arranged by size:
1524 1154 943 852 787 597 527 393
381 268 179 72 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2703 2703
Size: 7723
EcoRI G'AATT_C
Cuts at: 5032 5410 5032
Size: 378 7345
EcoRV GAT'ATC
Cuts at: 3736 3985 4780 3736
Size: 249 795 6679
Fragments arranged by size:
6679 795 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3107 3371 3647 4746 3107
Size: 264 276 1099 6084
Fragments arranged by size:
6084 1099 276 264
FspI TGC'GCA
Cuts at: 6229 6229
Size: 7723
HaeII r_GCGC'y
Cuts at: 1678 2052 3537 3592 6870 1678
Size: 374 1485 55 3278 2531
Fragments arranged by size:
3278 2531 1485 374 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2908 2940 3166
Size: 32 135 32 1052 32 252 32 226
Cuts at: 3166 3198 4766 4798 4963 4995 6364 6396 6438
Size: 32 1568 32 165 32 1369 32 42
Cuts at: 6438 6470 1373
Size: 32 2626
Fragments arranged by size:
2626 1568 1369 1052 252 226 165 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3436 4952 5067 5313 669
Size: 988 281 1498 1516 115 246 3079
Fragments arranged by size:
3079 1516 1498 988 281 246 115
HindIII A'AGCT_T
Cuts at: 748 4076 748
Size: 3328 4395
HpaI GTT'AAC
Cuts at: 5313 5313
Size: 7723
KpnI G_GTAC'C
Cuts at: 4236 5048 4236
Size: 812 6911
MfeI C'AATT_G
Cuts at: 5322 5322
Size: 7723
MluI A'CGCG_T
Cuts at: 7599 7599
Size: 7723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3119 6837 7021 1305
Size: 1814 3718 184 2007
Fragments arranged by size:
3718 2007 1814 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3396 4650 10
Size: 54 1425 343 1564 1254 3083
Fragments arranged by size:
3083 1564 1425 1254 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2805 3404 4656 5681 6040 6199 518
Size: 648 1639 599 1252 1025 359 159 2042
Fragments arranged by size:
2042 1639 1252 1025 648 599 359 159
MspA1I CmG'CkG
Cuts at: 1606 2174 2187 4628 5139 5816 6964 7209 1606
Size: 568 13 2441 511 677 1148 245 2120
Fragments arranged by size:
2441 2120 1148 677 568 511 245 13
NcoI C'CATG_G
Cuts at: 513 1065 3869 513
Size: 552 2804 4367
Fragments arranged by size:
4367 2804 552
NdeI CA'TA_TG
Cuts at: 387 2584 387
Size: 2197 5526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7723
NotI GC'GGCC_GC
Cuts at: 5170 5170
Size: 7723
NsiI A_TGCA'T
Cuts at: 3235 3235
Size: 7723
NspI r_CATG'y
Cuts at: 1132 1229 2641 3434 4657 5081 6626 1132
Size: 97 1412 793 1223 424 1545 2229
Fragments arranged by size:
2229 1545 1412 1223 793 424 97
PasI CC'CwG_GG
Cuts at: 1348 4237 1348
Size: 2889 4834
PciI A'CATG_T
Cuts at: 3430 4653 6622 3430
Size: 1223 1969 4531
Fragments arranged by size:
4531 1969 1223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2285 1108
Size: 1177 6546
PfoI T'CCnGG_A
Cuts at: 2015 2214 2547 2658 3678 4580 2015
Size: 199 333 111 1020 902 5158
Fragments arranged by size:
5158 1020 902 333 199 111
PmeI GTTT'AAAC
Cuts at: 5027 5027
Size: 7723
PmlI CAC'GTG
Cuts at: 4541 4541
Size: 7723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5792 5824 7305 7337 5792
Size: 32 1481 32 6178
Fragments arranged by size:
6178 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7723
PsiI TTA'TAA
Cuts at: 5293 5293
Size: 7723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3942 3974 3942
Size: 32 7691
PstI C_TGCA'G
Cuts at: 830 3214 5075 830
Size: 2384 1861 3478
Fragments arranged by size:
3478 2384 1861
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7723
PvuII CAG'CTG
Cuts at: 2174 4628 2174
Size: 2454 5269
SalI G'TCGA_C
Cuts at: 1936 5065 1936
Size: 3129 4594
SbfI CC_TGCA'GG
Cuts at: 5075 5075
Size: 7723
ScaI AGT'ACT
Cuts at: 3549 5971 7683 3549
Size: 2422 1712 3589
Fragments arranged by size:
3589 2422 1712
SexAI A'CCwGG_T
Cuts at: 2754 2754
Size: 7723
SfcI C'TryA_G
Cuts at: 826 1045 2683 3210 5071 6206 6887 7078 826
Size: 219 1638 527 1861 1135 681 191 1471
Fragments arranged by size:
1861 1638 1471 1135 681 527 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7723
SmaI CCC'GGG
Cuts at: 1872 5050 1872
Size: 3178 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4783 5827 6728 6990 820
Size: 197 926 2840 1044 901 262 1553
Fragments arranged by size:
2840 1553 1044 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5514 493
Size: 5021 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7723
SphI G_CATG'C
Cuts at: 1132 5081 1132
Size: 3949 3774
SspI AAT'ATT
Cuts at: 5 52 4795 5647 5
Size: 47 4743 852 2081
Fragments arranged by size:
4743 2081 852 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7723
StyI C'CwwG_G
Cuts at: 513 1065 2667 3198 3869 5162 513
Size: 552 1602 531 671 1293 3074
Fragments arranged by size:
3074 1602 1293 671 552 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 5750 5767 5920 6105 6550 325
Size: 1255 1419 2751 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1419 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 2785 2890
Size: 274 80 33 51 1443 335 473 105
Cuts at: 2890 3019 3118 3547 5969 7681 96
Size: 129 99 429 2422 1712 138
Fragments arranged by size:
2422 1712 1443 473 429 335 274 138
129 105 99 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 2480 2512 2719 2751 7683 7715 2063
Size: 32 385 32 207 32 4932 32 2071
Fragments arranged by size:
4932 2071 385 207 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7723
XbaI T'CTAG_A
Cuts at: 4903 5059 4903
Size: 156 7567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3518 3518
Size: 7723
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7723
XmaI C'CCGG_G
Cuts at: 1870 5048 1870
Size: 3178 4545
XmnI GAAnn'nnTTC
Cuts at: 4572 5852 4572
Size: 1280 6443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5531 276
Size: 53 83 186 936 3997 2468
Fragments arranged by size:
3997 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI