Restriction map for
pFN21AB8554
(Circular) (Six-base) MAPSORT of: pFN21AB8554.seq Check: 1735 from: 1 to: 7429
pFN21AB8554 7429 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4382 4382
Size: 7429
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5239 278
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4367 4772 1937
Size: 2430 405 4594
Fragments arranged by size:
4594 2430 405
Acc65I G'GTAC_C
Cuts at: 4750 4750
Size: 7429
AclI AA'CG_TT
Cuts at: 1563 5556 5929 1563
Size: 3993 373 3063
Fragments arranged by size:
3993 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3837 4087 4467 5492 6876 7144 1632
Size: 593 1612 250 380 1025 1384 268 1917
Fragments arranged by size:
1917 1612 1384 1025 593 380 268 250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7232
AflIII A'CryG_T
Cuts at: 1203 2316 6328 7305 1203
Size: 1113 4012 977 1327
Fragments arranged by size:
4012 1327 1113 977
AgeI A'CCGG_T
Cuts at: 6260 6260
Size: 7429
AhdI GACnn_n'nnGTC
Cuts at: 6158 6158
Size: 7429
AleI CACnn'nnGTG
Cuts at: 1166 3803 1166
Size: 2637 4792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7429
AlwNI CAG_nnn'CTG
Cuts at: 5172 6744 5172
Size: 1572 5857
ApaLI G'TGCA_C
Cuts at: 5486 6642 5486
Size: 1156 6273
ApoI r'AATT_y
Cuts at: 1493 2786 3086 3100 3352 4738 4964 5116 7225
Size: 1293 300 14 252 1386 226 152 2109
Cuts at: 7225 1493
Size: 1697
Fragments arranged by size:
2109 1697 1386 1293 300 252 226 152
14
AseI AT'TA_AT
Cuts at: 160 1032 2372 5983 160
Size: 872 1340 3611 1606
Fragments arranged by size:
3611 1606 1340 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
AvaI C'yCGr_G
Cuts at: 1870 1943 2583 2719 2829 3177 4754 1870
Size: 73 640 136 110 348 1577 4545
Fragments arranged by size:
4545 1577 640 348 136 110 73
AvrII C'CTAG_G
Cuts at: 3967 3967
Size: 7429
BamHI G'GATC_C
Cuts at: 2073 2939 4153 4759 5126 2073
Size: 866 1214 606 367 4376
Fragments arranged by size:
4376 1214 866 606 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 3760 4750 6205 618
Size: 325 217 627 401 1572 990 1455 1842
Fragments arranged by size:
1842 1572 1455 990 627 401 325 217
BanII G_rGCy'C
Cuts at: 1391 3520 4748 1391
Size: 2129 1228 4072
Fragments arranged by size:
4072 2129 1228
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3510 3955 928
Size: 971 1611 445 4402
Fragments arranged by size:
4402 1611 971 445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5620 5654 1903
Size: 34 3683 34 3678
Fragments arranged by size:
3683 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4031 4259 4283 5321 6531 3275
Size: 756 228 24 1038 1210 4173
Fragments arranged by size:
4173 1210 1038 756 228 24
BclI T'GATC_A
Cuts at: 1548 4095 1548
Size: 2547 4882
BfrBI ATG'CAT
Cuts at: 2534 2534
Size: 7429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6040 136
Size: 107 122 71 631 605 4368 1525
Fragments arranged by size:
4368 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2715 3268 7424 2715
Size: 553 4156 2720
Fragments arranged by size:
4156 2720 553
BlpI GC'TnA_GC
Cuts at: 4846 4846
Size: 7429
Bme1580I G_kGCm'C
Cuts at: 1790 5490 6646 1790
Size: 3700 1156 2573
Fragments arranged by size:
3700 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2651 4414 5160
Size: 416 53 83 399 24 1234 1763 746
Cuts at: 5160 6118 442
Size: 958 1753
Fragments arranged by size:
1763 1753 1234 958 746 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2802 3185 6089 1239
Size: 1013 550 383 2904 2579
Fragments arranged by size:
2904 2579 1013 550 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4208 5554 6419 6717 4208
Size: 1346 865 298 4920
Fragments arranged by size:
4920 1346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 3679 6092 882
Size: 617 660 1520 2413 2219
Fragments arranged by size:
2413 2219 1520 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 2319 2768 3874 5220 493
Size: 711 1115 449 1106 1346 2702
Fragments arranged by size:
2702 1346 1115 1106 711 449
BsaBI GATnn'nnATC
Cuts at: 4439 7423 4439
Size: 2984 4445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 3542 5236 5618
Size: 53 83 186 936 918 1091 1694 382
Cuts at: 5618 6253 275
Size: 635 1451
Fragments arranged by size:
1694 1451 1091 936 918 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5862 6260 6534 6681 7092 5862
Size: 398 274 147 411 6199
Fragments arranged by size:
6199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4122 4152 1178
Size: 30 165 30 2719 30 4455
Fragments arranged by size:
4455 2719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2984 3110 4579 1186
Size: 1798 126 1469 4036
Fragments arranged by size:
4036 1798 1469 126
BseYI C'CCAG_C
Cuts at: 6632 7128 6632
Size: 496 6933
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 3236 1163
Size: 573 648 852 5356
Fragments arranged by size:
5356 852 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4879 5640 6668 1540
Size: 275 186 2878 761 1028 2301
Fragments arranged by size:
2878 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 3879 4224 4724 5490 5575 6646 2324
Size: 1555 345 500 766 85 1071 3107
Fragments arranged by size:
3107 1555 1071 766 500 345 85
BsmI GAATG_Cn'
Cuts at: 3742 4940 5033 3742
Size: 1198 93 6138
Fragments arranged by size:
6138 1198 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 3520 3879 4224 4724 4748 5490
Size: 399 534 1196 359 345 500 24 742
Cuts at: 5490 5575 6646 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1196 1071 742 534 500 399 359
345 85 24
BspEI T'CCGG_A
Cuts at: 7092 7092
Size: 7429
BspHI T'CATG_A
Cuts at: 5318 7048 5318
Size: 1730 5699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4382 4784 844
Size: 3538 402 3489
Fragments arranged by size:
3538 3489 402
BsrBI CCG'CTC
Cuts at: 1394 1823 2694 4716 5316 1394
Size: 429 871 2022 600 3507
Fragments arranged by size:
3507 2022 871 600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 5924 6098 65
Size: 1183 1494 3182 174 1396
Fragments arranged by size:
3182 1494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6073 6260 1700
Size: 110 4263 187 2869
Fragments arranged by size:
4263 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4437 96
Size: 4341 3088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7429
BssSI C'ACGA_G
Cuts at: 5489 6501 5489
Size: 1012 6417
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3259 3708 794
Size: 2465 449 4515
Fragments arranged by size:
4515 2465 449
BstBI TT'CG_AA
Cuts at: 6233 6233
Size: 7429
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7429
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 4127 2128
Size: 1999 5430
BstYI r'GATC_y
Cuts at: 1972 2073 2413 2715 2939 3268 4153 4198 4759
Size: 101 340 302 224 329 885 45 561
Cuts at: 4759 5126 5511 5528 6980 7066 7078 7424 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 885 561 385 367 346 340
329 302 224 101 86 45 17 12
BstZ17I GTA'TAC
Cuts at: 4368 4368
Size: 7429
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 3237 513
Size: 552 939 615 618 4705
Fragments arranged by size:
4705 939 618 615 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3775 487
Size: 683 56 2549 4141
Fragments arranged by size:
4141 2549 683 56
BtsI GCAGTG_nn'
Cuts at: 2841 3370 3518 4945 5739 5759 2841
Size: 529 148 1427 794 20 4511
Fragments arranged by size:
4511 1427 794 529 148 20
ClaI AT'CG_AT
Cuts at: 1554 3031 1554
Size: 1477 5952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3784 3819 529
Size: 35 3220 35 4139
Fragments arranged by size:
4139 3220 35 35
DraI TTT'AAA
Cuts at: 4733 4733
Size: 7429
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6436 809
Size: 507 5120 1802
Fragments arranged by size:
5120 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3469 4876 5765
Size: 54 1425 50 275 18 1639 1407 889
Cuts at: 5765 7394 8
Size: 1629 43
Fragments arranged by size:
1639 1629 1425 1407 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4876 1537
Size: 275 3064 4090
Fragments arranged by size:
4090 3064 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 5359 1353
Size: 809 3197 3423
Fragments arranged by size:
3423 3197 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2583 6016 6390 6536 387
Size: 1064 138 994 3433 374 146 1280
Fragments arranged by size:
3433 1280 1064 994 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2252 2802 3185 3837 4087 4467
Size: 393 593 27 550 383 652 250 380
Cuts at: 4467 5492 6089 6876 7144 1239
Size: 1025 597 787 268 1524
Fragments arranged by size:
1524 1025 787 652 597 593 550 393
383 380 268 250 27
EcoO109I rG'GnC_Cy
Cuts at: 2269 3598 2269
Size: 1329 6100
EcoRI G'AATT_C
Cuts at: 3086 4738 5116 3086
Size: 1652 378 5399
Fragments arranged by size:
5399 1652 378
EcoRV GAT'ATC
Cuts at: 3421 3421
Size: 7429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3347 4585 3347
Size: 1238 6191
FspI TGC'GCA
Cuts at: 5935 5935
Size: 7429
HaeII r_GCGC'y
Cuts at: 1678 4064 6576 1678
Size: 2386 2512 2531
Fragments arranged by size:
2531 2512 2386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2564 2596 3641
Size: 32 135 32 734 32 226 32 1045
Cuts at: 3641 3673 4122 4154 4183 4215 4257 4289 6070
Size: 32 449 32 29 32 42 32 1781
Cuts at: 6070 6102 6144 6176 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1781 1045 734 449 226 135 42
42 32 32 32 32 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 4773 5019 669
Size: 988 281 2835 246 3079
Fragments arranged by size:
3079 2835 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7429
HpaI GTT'AAC
Cuts at: 5019 5019
Size: 7429
KpnI G_GTAC'C
Cuts at: 4754 4754
Size: 7429
MfeI C'AATT_G
Cuts at: 2250 3015 5028 2250
Size: 765 2013 4651
Fragments arranged by size:
4651 2013 765
MluI A'CGCG_T
Cuts at: 7305 7305
Size: 7429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 3979 6543 6727 1305
Size: 1005 1669 2564 184 2007
Fragments arranged by size:
2564 2007 1669 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5607
Fragments arranged by size:
5607 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2126 2644 3803 5387 5746 5905
Size: 648 918 42 518 1159 1584 359 159
Cuts at: 5905 518
Size: 2042
Fragments arranged by size:
2042 1584 1159 918 648 518 359 159
42
MspA1I CmG'CkG
Cuts at: 1606 4090 4189 4589 4845 5522 6670 6915 1606
Size: 2484 99 400 256 677 1148 245 2120
Fragments arranged by size:
2484 2120 1148 677 400 256 245 99
NcoI C'CATG_G
Cuts at: 513 1065 2004 2619 3237 513
Size: 552 939 615 618 4705
Fragments arranged by size:
4705 939 618 615 552
NdeI CA'TA_TG
Cuts at: 387 3944 387
Size: 3557 3872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7429
NotI GC'GGCC_GC
Cuts at: 4876 4876
Size: 7429
NsiI A_TGCA'T
Cuts at: 2536 2536
Size: 7429
NspI r_CATG'y
Cuts at: 1132 1229 3511 3982 4501 4787 6332 1132
Size: 97 2282 471 519 286 1545 2229
Fragments arranged by size:
2282 2229 1545 519 471 286 97
PasI CC'CwG_GG
Cuts at: 1348 3750 1348
Size: 2402 5027
PciI A'CATG_T
Cuts at: 6328 6328
Size: 7429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 2780 1108
Size: 1512 160 5757
Fragments arranged by size:
5757 1512 160
PfoI T'CCnGG_A
Cuts at: 3406 3406
Size: 7429
PmeI GTTT'AAAC
Cuts at: 4733 4733
Size: 7429
PmlI CAC'GTG
Cuts at: 2319 2768 2319
Size: 449 6980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5498 5530 7011 7043 5498
Size: 32 1481 32 5884
Fragments arranged by size:
5884 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 3598 2269
Size: 1329 6100
PshAI GACnn'nnGTC
Cuts at: 1613 3818 1613
Size: 2205 5224
PsiI TTA'TAA
Cuts at: 4526 4999 4526
Size: 473 6956
PspXI vC'TCGA_Gb
Cuts at: 2829 2829
Size: 7429
PstI C_TGCA'G
Cuts at: 830 2467 3554 4781 830
Size: 1637 1087 1227 3478
Fragments arranged by size:
3478 1637 1227 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7429
PvuII CAG'CTG
Cuts at: 4090 4589 4090
Size: 499 6930
SalI G'TCGA_C
Cuts at: 1936 4771 1936
Size: 2835 4594
SanDI GG'GwC_CC
Cuts at: 3598 3598
Size: 7429
SbfI CC_TGCA'GG
Cuts at: 4781 4781
Size: 7429
ScaI AGT'ACT
Cuts at: 5677 7389 5677
Size: 1712 5717
SexAI A'CCwGG_T
Cuts at: 2564 3642 2564
Size: 1078 6351
SfcI C'TryA_G
Cuts at: 826 1045 2463 3550 4692 4777 5912 6593 6784
Size: 219 1418 1087 1142 85 1135 681 191
Cuts at: 6784 826
Size: 1471
Fragments arranged by size:
1471 1418 1142 1135 1087 681 219 191
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7429
SmaI CCC'GGG
Cuts at: 1872 4756 1872
Size: 2884 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2583 2719 2829 4223 5533 6434
Size: 197 926 640 136 110 1394 1310 901
Cuts at: 6434 6696 820
Size: 262 1553
Fragments arranged by size:
1553 1394 1310 926 901 640 262 197
136 110
SnaBI TAC'GTA
Cuts at: 493 5220 493
Size: 4727 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7429
SphI G_CATG'C
Cuts at: 1132 4501 4787 1132
Size: 3369 286 3774
Fragments arranged by size:
3774 3369 286
SspI AAT'ATT
Cuts at: 5 52 3780 4032 5353 5
Size: 47 3728 252 1321 2081
Fragments arranged by size:
3728 2081 1321 252 47
StuI AGG'CCT
Cuts at: 1517 2705 1517
Size: 1188 6241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2619 3237 3967 4126 4512 4868
Size: 552 939 615 618 730 159 386 356
Cuts at: 4868 513
Size: 3074
Fragments arranged by size:
3074 939 730 618 615 552 386 356
159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 2434 2726 5456 5473 5626 5811
Size: 1255 708 146 292 2730 17 153 185
Cuts at: 5811 6256 325
Size: 445 1498
Fragments arranged by size:
2730 1498 1255 708 445 292 185 153
146 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3456 4292 4437
Size: 274 80 33 51 1443 1479 836 145
Cuts at: 4437 5675 7387 96
Size: 1238 1712 138
Fragments arranged by size:
1712 1479 1443 1238 836 274 145 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 2671 2703 2745 2777 7389 7421 2384
Size: 32 255 32 42 32 4612 32 2392
Fragments arranged by size:
4612 2392 255 42 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7429
XbaI T'CTAG_A
Cuts at: 4765 4765
Size: 7429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 3730 2293
Size: 1437 5992
XhoI C'TCGA_G
Cuts at: 1943 2583 2719 2829 1943
Size: 640 136 110 6543
Fragments arranged by size:
6543 640 136 110
XmaI C'CCGG_G
Cuts at: 1870 4754 1870
Size: 2884 4545
XmnI GAAnn'nnTTC
Cuts at: 5558 5558
Size: 7429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5237 276
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI