Restriction map for
pFN21AB8545
(Circular) (Six-base) MAPSORT of: pFN21AB8545.seq Check: 4296 from: 1 to: 7657
pFN21AB8545 7657 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5467 278
Size: 53 83 186 936 3931 2468
Fragments arranged by size:
3931 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2250 3850 4101 5000 1937
Size: 313 1600 251 899 4594
Fragments arranged by size:
4594 1600 899 313 251
Acc65I G'GTAC_C
Cuts at: 4978 4978
Size: 7657
AclI AA'CG_TT
Cuts at: 1563 5784 6157 1563
Size: 4221 373 3063
Fragments arranged by size:
4221 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3589 5720 7104 7372 1632
Size: 941 1016 2131 1384 268 1917
Fragments arranged by size:
2131 1917 1384 1016 941 268
AfeI AGC'GCT
Cuts at: 4771 4771
Size: 7657
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7460
AflIII A'CryG_T
Cuts at: 1203 6556 7533 1203
Size: 5353 977 1327
Fragments arranged by size:
5353 1327 977
AgeI A'CCGG_T
Cuts at: 6488 6488
Size: 7657
AhdI GACnn_n'nnGTC
Cuts at: 6386 6386
Size: 7657
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7657
AlwNI CAG_nnn'CTG
Cuts at: 2539 3818 5400 6972 2539
Size: 1279 1582 1572 3224
Fragments arranged by size:
3224 1582 1572 1279
ApaLI G'TGCA_C
Cuts at: 5714 6870 5714
Size: 1156 6501
ApoI r'AATT_y
Cuts at: 1493 3300 3344 3486 4125 4469 4850 4966 5192
Size: 1807 44 142 639 344 381 116 226
Cuts at: 5192 5344 7453 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1807 1697 639 381 344 226 152
142 116 44
AseI AT'TA_AT
Cuts at: 160 1032 3540 6211 160
Size: 872 2508 2671 1606
Fragments arranged by size:
2671 2508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
AvaI C'yCGr_G
Cuts at: 1870 1943 4089 4884 4982 1870
Size: 73 2146 795 98 4545
Fragments arranged by size:
4545 2146 795 98 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2109 2142 2471 2504 2109
Size: 33 329 33 7262
Fragments arranged by size:
7262 329 33 33
BamHI G'GATC_C
Cuts at: 4987 5354 4987
Size: 367 7290
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3599 4698 4978 6433 618
Size: 325 217 627 1812 1099 280 1455 1842
Fragments arranged by size:
1842 1812 1455 1099 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 3526 4976 1391
Size: 2135 1450 4072
Fragments arranged by size:
4072 2135 1450
BbeI G_GCGC'C
Cuts at: 4702 4702
Size: 7657
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 3466 928
Size: 971 161 1406 5119
Fragments arranged by size:
5119 1406 971 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4703 4737 5848 5882 1903
Size: 34 2766 34 1111 34 3678
Fragments arranged by size:
3678 2766 1111 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4641 4890 5549 6759 4641
Size: 249 659 1210 5539
Fragments arranged by size:
5539 1210 659 249
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6268 136
Size: 107 122 71 631 605 4596 1525
Fragments arranged by size:
4596 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3938 7652 3938
Size: 3714 3943
BlpI GC'TnA_GC
Cuts at: 2532 5074 2532
Size: 2542 5115
Bme1580I G_kGCm'C
Cuts at: 1790 5718 6874 1790
Size: 3928 1156 2573
Fragments arranged by size:
3928 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2927 1318
Size: 1609 6048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5388 6346 442
Size: 416 53 83 399 24 3971 958 1753
Fragments arranged by size:
3971 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3681 4713 1056
Size: 2625 1032 4000
Fragments arranged by size:
4000 2625 1032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 2528 3657 3714 6317 1239
Size: 1113 176 1129 57 2603 2579
Fragments arranged by size:
2603 2579 1129 1113 176 57
Bpu10I CC'TnA_GC
Cuts at: 2737 2737
Size: 7657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2760 3597 3734 3947 5782 6647 6945 2760
Size: 837 137 213 1835 865 298 3472
Fragments arranged by size:
3472 1835 865 837 298 213 137
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3333 6320 882
Size: 617 1834 2987 2219
Fragments arranged by size:
2987 2219 1834 617
BsaAI yAC'GTr
Cuts at: 493 1204 3290 5448 493
Size: 711 2086 2158 2702
Fragments arranged by size:
2702 2158 2086 711
BsaBI GATnn'nnATC
Cuts at: 7651 7651
Size: 7657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4699 5464 5846
Size: 53 83 186 936 476 2690 765 382
Cuts at: 5846 6481 275
Size: 635 1451
Fragments arranged by size:
2690 1451 936 765 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6090 6488 6762 6909 7320 6090
Size: 398 274 147 411 6427
Fragments arranged by size:
6427 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3073 3103 4068 4098 1178
Size: 30 165 30 1670 30 965 30 4737
Fragments arranged by size:
4737 1670 965 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3516 3717 3879 4925 1186
Size: 2330 201 162 1046 3918
Fragments arranged by size:
3918 2330 1046 201 162
BseYI C'CCAG_C
Cuts at: 6860 7356 6860
Size: 496 7161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2487 1163
Size: 573 411 340 6333
Fragments arranged by size:
6333 573 411 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5107 5868 6896 1540
Size: 275 186 3106 761 1028 2301
Fragments arranged by size:
3106 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3526 5718 5803 6874 3526
Size: 2192 85 1071 4309
Fragments arranged by size:
4309 2192 1071 85
BsmI GAATG_Cn'
Cuts at: 2583 5168 5261 2583
Size: 2585 93 4979
Fragments arranged by size:
4979 2585 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3526 4976 5718 5803 6874 1391
Size: 399 1736 1450 742 85 1071 2174
Fragments arranged by size:
2174 1736 1450 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7320 7320
Size: 7657
BspHI T'CATG_A
Cuts at: 3108 5546 7276 3108
Size: 2438 1730 3489
Fragments arranged by size:
3489 2438 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5012 844
Size: 4168 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5544 1394
Size: 429 3721 3507
Fragments arranged by size:
3721 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4941 6152 6326 65
Size: 1183 3693 1211 174 1396
Fragments arranged by size:
3693 1396 1211 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4226 6301 6488 1700
Size: 110 2416 2075 187 2869
Fragments arranged by size:
2869 2416 2075 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7657
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7657
BssSI C'ACGA_G
Cuts at: 3741 5717 6729 3741
Size: 1976 1012 4669
Fragments arranged by size:
4669 1976 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2708 794
Size: 1914 5743
BstBI TT'CG_AA
Cuts at: 6461 6461
Size: 7657
BstEII G'GTnAC_C
Cuts at: 1184 3430 1184
Size: 2246 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 3383 3383
Size: 7657
BstYI r'GATC_y
Cuts at: 1972 2335 2498 3938 4987 5354 5739 5756 7208
Size: 363 163 1440 1049 367 385 17 1452
Cuts at: 7208 7294 7306 7652 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1440 1049 385 367 363 346
163 86 17 12
BstZ17I GTA'TAC
Cuts at: 3851 3851
Size: 7657
BtgI C'CryG_G
Cuts at: 513 1065 2004 2521 513
Size: 552 939 517 5649
Fragments arranged by size:
5649 939 552 517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6918
Fragments arranged by size:
6918 683 56
BtsI GCAGTG_nn'
Cuts at: 3068 5173 5967 5987 3068
Size: 2105 794 20 4738
Fragments arranged by size:
4738 2105 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7622
DraI TTT'AAA
Cuts at: 2817 4961 2817
Size: 2144 5513
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6664 809
Size: 507 5348 1802
Fragments arranged by size:
5348 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 5104 5993
Size: 54 1425 50 275 18 1387 1887 889
Cuts at: 5993 7622 8
Size: 1629 43
Fragments arranged by size:
1887 1629 1425 1387 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5104 1537
Size: 275 3292 4090
Fragments arranged by size:
4090 3292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 3991 4584 4902 5587 1353
Size: 2212 426 593 318 685 3423
Fragments arranged by size:
3423 2212 685 593 426 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6244 6618 6764 387
Size: 1064 138 4655 374 146 1280
Fragments arranged by size:
4655 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3524 3524
Size: 7657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2528 2573 3589 3657 3714 5720
Size: 393 720 176 45 1016 68 57 2006
Cuts at: 5720 6317 7104 7372 1239
Size: 597 787 268 1524
Fragments arranged by size:
2006 1524 1016 787 720 597 393 268
176 68 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3894 3894
Size: 7657
EcoO109I rG'GnC_Cy
Cuts at: 2422 4234 4528 2422
Size: 1812 294 5551
Fragments arranged by size:
5551 1812 294
EcoRI G'AATT_C
Cuts at: 4966 5344 4966
Size: 378 7279
EcoRV GAT'ATC
Cuts at: 2146 2940 3025 2146
Size: 794 85 6778
Fragments arranged by size:
6778 794 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2259 3023 2259
Size: 764 6893
FspI TGC'GCA
Cuts at: 6163 6163
Size: 7657
HaeII r_GCGC'y
Cuts at: 1678 4702 4773 6804 1678
Size: 3024 71 2031 2531
Fragments arranged by size:
3024 2531 2031 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 3155 3187 3311
Size: 32 135 32 1057 32 494 32 124
Cuts at: 3311 3343 3923 3955 4867 4899 6298 6330 6372
Size: 32 580 32 912 32 1399 32 42
Cuts at: 6372 6404 1373
Size: 32 2626
Fragments arranged by size:
2626 1399 1057 912 580 494 135 124
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5001 5247 669
Size: 988 281 3063 246 3079
Fragments arranged by size:
3079 3063 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7657
HpaI GTT'AAC
Cuts at: 5247 5247
Size: 7657
KasI G'GCGC_C
Cuts at: 4698 4698
Size: 7657
KpnI G_GTAC'C
Cuts at: 4982 4982
Size: 7657
MfeI C'AATT_G
Cuts at: 2435 5256 2435
Size: 2821 4836
MluI A'CGCG_T
Cuts at: 7533 7533
Size: 7657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2787 3059 6771 6955 1305
Size: 732 750 272 3712 184 2007
Fragments arranged by size:
3712 2007 750 732 272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3219 10
Size: 54 1425 343 1387 4448
Fragments arranged by size:
4448 1425 1387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 4257 5615 5974 6133 518
Size: 648 1410 1681 1358 359 159 2042
Fragments arranged by size:
2042 1681 1410 1358 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 5073 5750 6898 7143 1606
Size: 3467 677 1148 245 2120
Fragments arranged by size:
3467 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 4699 4699
Size: 7657
NcoI C'CATG_G
Cuts at: 513 1065 2004 2521 513
Size: 552 939 517 5649
Fragments arranged by size:
5649 939 552 517
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7657
NheI G'CTAG_C
Cuts at: 1052 3677 4709 1052
Size: 2625 1032 4000
Fragments arranged by size:
4000 2625 1032
NotI GC'GGCC_GC
Cuts at: 5104 5104
Size: 7657
NspI r_CATG'y
Cuts at: 1132 1229 3391 4182 4202 5015 6560 1132
Size: 97 2162 791 20 813 1545 2229
Fragments arranged by size:
2229 2162 1545 813 791 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7657
PciI A'CATG_T
Cuts at: 6556 6556
Size: 7657
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7657
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 7657
PmeI GTTT'AAAC
Cuts at: 4961 4961
Size: 7657
PmlI CAC'GTG
Cuts at: 3290 3290
Size: 7657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5726 5758 7239 7271 5726
Size: 32 1481 32 6112
Fragments arranged by size:
6112 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4234 4234
Size: 7657
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7657
PsiI TTA'TAA
Cuts at: 5227 5227
Size: 7657
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4622 4654 4881 4913 4622
Size: 32 227 32 7366
Fragments arranged by size:
7366 227 32 32
PstI C_TGCA'G
Cuts at: 830 2052 2307 5009 830
Size: 1222 255 2702 3478
Fragments arranged by size:
3478 2702 1222 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7657
SacI G_AGCT'C
Cuts at: 3526 3526
Size: 7657
SalI G'TCGA_C
Cuts at: 1936 4999 1936
Size: 3063 4594
SapI GCTCTTCn'nnn_
Cuts at: 3565 3565
Size: 7657
SbfI CC_TGCA'GG
Cuts at: 5009 5009
Size: 7657
ScaI AGT'ACT
Cuts at: 5905 7617 5905
Size: 1712 5945
SfcI C'TryA_G
Cuts at: 826 1045 2048 2303 3582 3630 3687 4515 4647
Size: 219 1003 255 1279 48 57 828 132
Cuts at: 4647 5005 6140 6821 7012 826
Size: 358 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 1003 828 681 358 255
219 191 132 57 48
SfoI GGC'GCC
Cuts at: 4700 4700
Size: 7657
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7657
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7657
SmaI CCC'GGG
Cuts at: 1872 4984 1872
Size: 3112 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2775 3612 3713 3926 4089 4884
Size: 197 926 832 837 101 213 163 795
Cuts at: 4884 5761 6662 6924 820
Size: 877 901 262 1553
Fragments arranged by size:
1553 926 901 877 837 832 795 262
213 197 163 101
SnaBI TAC'GTA
Cuts at: 493 5448 493
Size: 4955 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7657
SphI G_CATG'C
Cuts at: 1132 5015 1132
Size: 3883 3774
SspI AAT'ATT
Cuts at: 5 52 3538 3544 5581 5
Size: 47 3486 6 2037 2081
Fragments arranged by size:
3486 2081 2037 47 6
StuI AGG'CCT
Cuts at: 1517 3900 1517
Size: 2383 5274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2360 2521 4531 4634 4702 4892
Size: 552 939 356 161 2010 103 68 190
Cuts at: 4892 5096 513
Size: 204 3074
Fragments arranged by size:
3074 2010 939 552 356 204 190 161
103 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4758 5684 5701 5854 6039 6484 325
Size: 1255 3178 926 17 153 185 445 1498
Fragments arranged by size:
3178 1498 1255 926 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2960 5903 7615
Size: 274 80 33 51 1443 983 2943 1712
Cuts at: 7615 96
Size: 138
Fragments arranged by size:
2943 1712 1443 983 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 4068 4100 7617 7649 3073
Size: 32 963 32 3517 32 3081
Fragments arranged by size:
3517 3081 963 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7657
XbaI T'CTAG_A
Cuts at: 4993 4993
Size: 7657
XhoI C'TCGA_G
Cuts at: 1943 4089 4884 1943
Size: 2146 795 4716
Fragments arranged by size:
4716 2146 795
XmaI C'CCGG_G
Cuts at: 1870 4982 1870
Size: 3112 4545
XmnI GAAnn'nnTTC
Cuts at: 5786 5786
Size: 7657
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5465 276
Size: 53 83 186 936 3931 2468
Fragments arranged by size:
3931 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspOMI PspXI PvuII RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI