Restriction map for
pFN21AB8543
(Circular) (Six-base) MAPSORT of: pFN21AB8543.seq Check: 2897 from: 1 to: 7645
pFN21AB8543 7645 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3962 3962
Size: 7645
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5455 278
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4988 1937
Size: 3051 4594
Acc65I G'GTAC_C
Cuts at: 4966 4966
Size: 7645
AclI AA'CG_TT
Cuts at: 1563 5772 6145 1563
Size: 4209 373 3063
Fragments arranged by size:
4209 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3258 3371 3500 5708 7092 7360 1632
Size: 1302 324 113 129 2208 1384 268 1917
Fragments arranged by size:
2208 1917 1384 1302 324 268 129 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7448
AflIII A'CryG_T
Cuts at: 1203 4329 4645 6544 7521 1203
Size: 3126 316 1899 977 1327
Fragments arranged by size:
3126 1899 1327 977 316
AgeI A'CCGG_T
Cuts at: 6476 6476
Size: 7645
AhdI GACnn_n'nnGTC
Cuts at: 2360 6374 2360
Size: 4014 3631
AleI CACnn'nnGTG
Cuts at: 1166 3593 3880 1166
Size: 2427 287 4931
Fragments arranged by size:
4931 2427 287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4411 1184
Size: 3227 4418
AlwNI CAG_nnn'CTG
Cuts at: 2227 2513 3501 3739 4051 4903 5388 6960 2227
Size: 286 988 238 312 852 485 1572 2912
Fragments arranged by size:
2912 1572 988 852 485 312 286 238
ApaI G_GGCC'C
Cuts at: 2087 2456 3416 3559 3733 3826 4239 2087
Size: 369 960 143 174 93 413 5493
Fragments arranged by size:
5493 960 413 369 174 143 93
ApaLI G'TGCA_C
Cuts at: 5702 6858 5702
Size: 1156 6489
ApoI r'AATT_y
Cuts at: 1493 2880 4670 4954 5180 5332 7441 1493
Size: 1387 1790 284 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 1387 284 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6199 160
Size: 872 5167 1606
Fragments arranged by size:
5167 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2210 3552 3776 4970 1870
Size: 73 135 132 1342 224 1194 4545
Fragments arranged by size:
4545 1342 1194 224 135 132 73
BamHI G'GATC_C
Cuts at: 4975 5342 4975
Size: 367 7278
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2330 3374 3484 3781
Size: 325 217 627 405 138 1044 110 297
Cuts at: 3781 4244 4966 6421 618
Size: 463 722 1455 1842
Fragments arranged by size:
1842 1455 1044 722 627 463 405 325
297 217 138 110
BanII G_rGCy'C
Cuts at: 1391 2037 2087 2144 2267 2352 2456 2484 2799
Size: 646 50 57 123 85 104 28 315
Cuts at: 2799 2877 3257 3416 3471 3559 3733 3777 3826
Size: 78 380 159 55 88 174 44 49
Cuts at: 3826 4093 4239 4323 4964 1391
Size: 267 146 84 641 4072
Fragments arranged by size:
4072 646 641 380 315 267 174 159
146 123 104 88 85 84 78 57
55 50 49 44 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 2976 3437 928
Size: 971 1023 54 461 5136
Fragments arranged by size:
5136 1023 971 461 54
BbvCI CC'TCA_GC
Cuts at: 4032 4301 4032
Size: 269 7376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3767 3801 5836 5870 1903
Size: 34 1830 34 2035 34 3678
Fragments arranged by size:
3678 2035 1830 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5537 6747 5537
Size: 1210 6435
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7645
BfrBI ATG'CAT
Cuts at: 4398 4398
Size: 7645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2834 3136 3206
Size: 107 122 71 631 605 1162 302 70
Cuts at: 3206 3420 6256 136
Size: 214 2836 1525
Fragments arranged by size:
2836 1525 1162 631 605 302 214 122
107 71 70
BglII A'GATC_T
Cuts at: 4558 7640 4558
Size: 3082 4563
BlpI GC'TnA_GC
Cuts at: 3809 4828 5062 3809
Size: 1019 234 6392
Fragments arranged by size:
6392 1019 234
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2195 2323 2456 3379 3416 3489 3559
Size: 297 108 128 133 923 37 73 70
Cuts at: 3559 3733 3826 4239 5706 6862 1790
Size: 174 93 413 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 923 413 297 174 133
128 108 93 73 70 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 2393 2777
Size: 416 53 83 399 24 853 123 384
Cuts at: 2777 5376 6334 442
Size: 2599 958 1753
Fragments arranged by size:
2599 1753 958 853 416 399 384 123
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3875 1056
Size: 2819 4826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2816 2411
Size: 405 7240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2195 2441 2570 2628 3165 3294 3313
Size: 812 144 246 129 58 537 129 19
Cuts at: 3313 3338 3347 3478 4073 4178 4199 4479 4823
Size: 25 9 131 595 105 21 280 344
Cuts at: 4823 6305 1239
Size: 1482 2579
Fragments arranged by size:
2579 1482 812 595 537 344 280 246
144 131 129 129 105 58 25 21
19 9
Bpu10I CC'TnA_GC
Cuts at: 3251 4032 4188 4216 4301 3251
Size: 781 156 28 85 6595
Fragments arranged by size:
6595 781 156 85 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5770 6635 6933 5770
Size: 865 298 6482
Fragments arranged by size:
6482 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 4154 6308 882
Size: 617 885 1770 2154 2219
Fragments arranged by size:
2219 2154 1770 885 617
BsaAI yAC'GTr
Cuts at: 493 1204 5436 493
Size: 711 4232 2702
Fragments arranged by size:
4232 2702 711
BsaBI GATnn'nnATC
Cuts at: 7639 7639
Size: 7645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5452 5834 6469 275
Size: 53 83 186 936 3919 382 635 1451
Fragments arranged by size:
3919 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4908 6078 6476 6750 6897 7308 4908
Size: 1170 398 274 147 411 5245
Fragments arranged by size:
5245 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4545 4575 4619 4649 1178
Size: 30 165 30 3142 30 44 30 4174
Fragments arranged by size:
4174 3142 165 44 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3297 3656 3659 4001 4364 4476 1186
Size: 2111 359 3 342 363 112 4355
Fragments arranged by size:
4355 2111 363 359 342 112 3
BseYI C'CCAG_C
Cuts at: 2104 2338 2449 2748 2788 3625 4638 4725 6848
Size: 234 111 299 40 837 1013 87 2123
Cuts at: 6848 7344 2104
Size: 496 2405
Fragments arranged by size:
2405 2123 1013 837 496 299 234 111
87 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 3525 3773 3906 3928 4306 4496
Size: 573 1014 775 248 133 22 378 190
Cuts at: 4496 1163
Size: 4312
Fragments arranged by size:
4312 1014 775 573 378 248 190 133
22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2432 5095 5856 6884 1540
Size: 275 186 45 386 2663 761 1028 2301
Fragments arranged by size:
2663 2301 1028 761 386 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2484 2877 3777 4185 4323 5706 5791 6862 2484
Size: 393 900 408 138 1383 85 1071 3267
Fragments arranged by size:
3267 1383 1071 900 408 393 138 85
BsmI GAATG_Cn'
Cuts at: 5156 5249 5156
Size: 93 7552
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 4204 2417
Size: 1787 5858
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2087 2144 2195 2267 2323 2352
Size: 399 247 50 57 51 72 56 29
Cuts at: 2352 2456 2484 2799 2877 3257 3379 3416 3471
Size: 104 28 315 78 380 122 37 55
Cuts at: 3471 3489 3559 3733 3777 3826 4093 4185 4239
Size: 18 70 174 44 49 267 92 54
Cuts at: 4239 4323 4964 5706 5791 6862 1391
Size: 84 641 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 399 380 315 267
247 174 122 104 92 85 84 78
72 70 57 56 55 54 51 50
49 44 37 29 28 18
BspEI T'CCGG_A
Cuts at: 7308 7308
Size: 7645
BspHI T'CATG_A
Cuts at: 3769 4795 5534 7264 3769
Size: 1026 739 1730 4150
Fragments arranged by size:
4150 1730 1026 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 3747 3962 5000 844
Size: 1878 1025 215 1038 3489
Fragments arranged by size:
3489 1878 1038 1025 215
BsrBI CCG'CTC
Cuts at: 1394 1823 5532 1394
Size: 429 3709 3507
Fragments arranged by size:
3709 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2933 6140 6314 65
Size: 1183 1685 3207 174 1396
Fragments arranged by size:
3207 1685 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2152 2431 3030 3567 4013 4247
Size: 110 282 60 279 599 537 446 234
Cuts at: 4247 6289 6476 1700
Size: 2042 187 2869
Fragments arranged by size:
2869 2042 599 537 446 282 279 234
187 110 60
BsrGI T'GTAC_A
Cuts at: 96 3231 96
Size: 3135 4510
BssHII G'CGCG_C
Cuts at: 1925 2238 1925
Size: 313 7332
BssSI C'ACGA_G
Cuts at: 5705 6717 5705
Size: 1012 6633
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 4903 794
Size: 1652 2457 3536
Fragments arranged by size:
3536 2457 1652
BstBI TT'CG_AA
Cuts at: 6449 6449
Size: 7645
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7645
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4085 2011
Size: 2074 5571
BstYI r'GATC_y
Cuts at: 1972 3102 3904 4098 4152 4558 4975 5342 5727
Size: 1130 802 194 54 406 417 367 385
Cuts at: 5727 5744 7196 7282 7294 7640 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1130 802 417 406 385 367
346 194 86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2123 2123
Size: 7645
BtgI C'CryG_G
Cuts at: 513 1065 2004 2087 2411 2485 2544 2641 3263
Size: 552 939 83 324 74 59 97 622
Cuts at: 3263 4231 4845 513
Size: 968 614 3313
Fragments arranged by size:
3313 968 939 622 614 552 324 97
83 74 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2890 487
Size: 683 56 1664 5242
Fragments arranged by size:
5242 1664 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2321 3403 3828 5161 5955 5975 2070
Size: 251 1082 425 1333 794 20 3740
Fragments arranged by size:
3740 1333 1082 794 425 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3424 3459 529
Size: 35 2860 35 4715
Fragments arranged by size:
4715 2860 35 35
DraI TTT'AAA
Cuts at: 3154 4949 3154
Size: 1795 5850
DraIII CAC_nnn'GTG
Cuts at: 4284 4284
Size: 7645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6652 809
Size: 507 5336 1802
Fragments arranged by size:
5336 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2429 3207
Size: 54 1425 50 275 18 260 339 778
Cuts at: 3207 3714 4263 5092 5981 7610 8
Size: 507 549 829 889 1629 43
Fragments arranged by size:
1629 1425 889 829 778 549 507 339
275 260 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2429 5092 1537
Size: 275 617 2663 4090
Fragments arranged by size:
4090 2663 617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 2660 5575 1353
Size: 840 467 2915 3423
Fragments arranged by size:
3423 2915 840 467
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 6232 6606 6752 387
Size: 1064 138 525 4118 374 146 1280
Fragments arranged by size:
4118 1280 1064 525 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2875 3775 4321 2482
Size: 393 900 546 5806
Fragments arranged by size:
5806 900 546 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2195 2441 2570 2628 2934 3165
Size: 393 419 144 246 129 58 306 231
Cuts at: 3165 3258 3294 3313 3338 3347 3371 3478 3500
Size: 93 36 19 25 9 24 107 22
Cuts at: 3500 4073 4178 4199 4479 4823 5708 6305 7092
Size: 573 105 21 280 344 885 597 787
Cuts at: 7092 7360 1239
Size: 268 1524
Fragments arranged by size:
1524 885 787 597 573 419 393 344
306 280 268 246 231 144 129 107
105 93 58 36 25 24 22 21
19 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 3674 3848 3946 4031 2657
Size: 1017 174 98 85 6271
Fragments arranged by size:
6271 1017 174 98 85
EcoO109I rG'GnC_Cy
Cuts at: 2026 2083 2207 2271 2342 2734 2752 2771 3412
Size: 57 124 64 71 392 18 19 641
Cuts at: 3412 3413 3679 3736 3823 4236 2026
Size: 1 266 57 87 413 5435
Fragments arranged by size:
5435 641 413 392 266 124 87 71
64 57 57 19 18 1
EcoRI G'AATT_C
Cuts at: 2880 4954 5332 2880
Size: 2074 378 5193
Fragments arranged by size:
5193 2074 378
FspI TGC'GCA
Cuts at: 6151 6151
Size: 7645
HaeII r_GCGC'y
Cuts at: 1678 2012 2159 2312 2819 6792 1678
Size: 334 147 153 507 3973 2531
Fragments arranged by size:
3973 2531 507 334 153 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2346 2378 4545 4577 4619
Size: 32 135 32 774 32 2167 32 42
Cuts at: 4619 4651 4733 4765 6286 6318 6360 6392 1373
Size: 32 82 32 1521 32 42 32 2626
Fragments arranged by size:
2626 2167 1521 774 135 82 42 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4989 5235 669
Size: 988 281 3051 246 3079
Fragments arranged by size:
3079 3051 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7645
HpaI GTT'AAC
Cuts at: 5235 5235
Size: 7645
KpnI G_GTAC'C
Cuts at: 4970 4970
Size: 7645
MfeI C'AATT_G
Cuts at: 3087 5244 3087
Size: 2157 5488
MluI A'CGCG_T
Cuts at: 4645 7521 4645
Size: 2876 4769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2066 4066 6759 6943 1305
Size: 761 2000 2693 184 2007
Fragments arranged by size:
2693 2007 2000 761 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3716 4265 10
Size: 54 1425 343 1884 549 3390
Fragments arranged by size:
3390 1884 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3593 3710 3880 4328 4334 5603 5962
Size: 648 2427 117 170 448 6 1269 359
Cuts at: 5962 6121 518
Size: 159 2042
Fragments arranged by size:
2427 2042 1269 648 448 359 170 159
117 6
MspA1I CmG'CkG
Cuts at: 1606 2371 2413 2487 2788 3593 3693 3805 4729
Size: 765 42 74 301 805 100 112 924
Cuts at: 4729 4775 5061 5738 6886 7131 1606
Size: 46 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 805 765 677 301 286
245 112 100 74 46 42
NaeI GCC'GGC
Cuts at: 2094 2154 2433 3569 4015 2094
Size: 60 279 1136 446 5724
Fragments arranged by size:
5724 1136 446 279 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 3263 4231 4845 513
Size: 552 939 1259 968 614 3313
Fragments arranged by size:
3313 1259 968 939 614 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7645
NgoMIV G'CCGG_C
Cuts at: 2092 2152 2431 3567 4013 2092
Size: 60 279 1136 446 5724
Fragments arranged by size:
5724 1136 446 279 60
NheI G'CTAG_C
Cuts at: 1052 3871 1052
Size: 2819 4826
NotI GC'GGCC_GC
Cuts at: 5092 5092
Size: 7645
NsiI A_TGCA'T
Cuts at: 4400 4400
Size: 7645
NspI r_CATG'y
Cuts at: 1132 1229 4333 5003 6548 1132
Size: 97 3104 670 1545 2229
Fragments arranged by size:
3104 2229 1545 670 97
PasI CC'CwG_GG
Cuts at: 1348 2456 3048 3844 1348
Size: 1108 592 796 5149
Fragments arranged by size:
5149 1108 796 592
PciI A'CATG_T
Cuts at: 4329 6544 4329
Size: 2215 5430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2513 3089 3291 3425 1108
Size: 1405 576 202 134 5328
Fragments arranged by size:
5328 1405 576 202 134
PfoI T'CCnGG_A
Cuts at: 2028 2404 3057 3105 4155 4563 2028
Size: 376 653 48 1050 408 5110
Fragments arranged by size:
5110 1050 653 408 376 48
PmeI GTTT'AAAC
Cuts at: 4949 4949
Size: 7645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5714 5746 7227 7259 5714
Size: 32 1481 32 6100
Fragments arranged by size:
6100 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2207 2734 2752 2771 3679 3736 2026
Size: 181 527 18 19 908 57 5935
Fragments arranged by size:
5935 908 527 181 57 19 18
PshAI GACnn'nnGTC
Cuts at: 1613 2523 1613
Size: 910 6735
PsiI TTA'TAA
Cuts at: 5215 5215
Size: 7645
PspOMI G'GGCC_C
Cuts at: 2083 2452 3412 3555 3729 3822 4235 2083
Size: 369 960 143 174 93 413 5493
Fragments arranged by size:
5493 960 413 369 174 143 93
PspXI vC'TCGA_Gb
Cuts at: 3776 3776
Size: 7645
PstI C_TGCA'G
Cuts at: 830 2134 3540 3577 4065 4137 4390 4863 4997
Size: 1304 1406 37 488 72 253 473 134
Cuts at: 4997 830
Size: 3478
Fragments arranged by size:
3478 1406 1304 488 473 253 134 72
37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7645
PvuII CAG'CTG
Cuts at: 3593 3693 4729 4775 3593
Size: 100 1036 46 6463
Fragments arranged by size:
6463 1036 100 46
RsrII CG'GwC_CG
Cuts at: 2354 2408 2354
Size: 54 7591
SacI G_AGCT'C
Cuts at: 2484 2877 3777 4323 2484
Size: 393 900 546 5806
Fragments arranged by size:
5806 900 546 393
SacII CC_GC'GG
Cuts at: 2414 2488 2414
Size: 74 7571
SalI G'TCGA_C
Cuts at: 1936 4987 1936
Size: 3051 4594
SanDI GG'GwC_CC
Cuts at: 2207 2771 2207
Size: 564 7081
SapI GCTCTTCn'nnn_
Cuts at: 2193 2193
Size: 7645
SbfI CC_TGCA'GG
Cuts at: 2134 4997 2134
Size: 2863 4782
ScaI AGT'ACT
Cuts at: 5893 7605 5893
Size: 1712 5933
SexAI A'CCwGG_T
Cuts at: 4819 4819
Size: 7645
SfcI C'TryA_G
Cuts at: 826 1045 2130 2995 3454 3536 3573 4061 4133
Size: 219 1085 865 459 82 37 488 72
Cuts at: 4133 4386 4859 4993 6128 6809 7000 826
Size: 253 473 134 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 865 681 488 473 459
253 219 191 134 82 72 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7645
SmaI CCC'GGG
Cuts at: 1872 2080 2212 3554 4972 1872
Size: 208 132 1342 1418 4545
Fragments arranged by size:
4545 1418 1342 208 132
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3776 5749 6650 6912 820
Size: 197 926 1833 1973 901 262 1553
Fragments arranged by size:
1973 1833 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5436 493
Size: 4943 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7645
SphI G_CATG'C
Cuts at: 1132 5003 1132
Size: 3871 3774
SspI AAT'ATT
Cuts at: 5 52 5569 5
Size: 47 5517 2081
Fragments arranged by size:
5517 2081 47
StuI AGG'CCT
Cuts at: 1517 2128 3315 3323 4221 1517
Size: 611 1187 8 898 4941
Fragments arranged by size:
4941 1187 898 611 8
StyI C'CwwG_G
Cuts at: 513 1065 2004 2647 3263 3324 3463 4231 4266
Size: 552 939 643 616 61 139 768 35
Cuts at: 4266 4845 5084 513
Size: 579 239 3074
Fragments arranged by size:
3074 939 768 643 616 579 552 239
139 61 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2266 2659 2665 3233 3617 4115
Size: 1255 480 206 393 6 568 384 498
Cuts at: 4115 5672 5689 5842 6027 6472 325
Size: 1557 17 153 185 445 1498
Fragments arranged by size:
1557 1498 1255 568 498 480 445 393
384 206 185 153 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3231 5891 7603
Size: 274 80 33 51 1443 1254 2660 1712
Cuts at: 7603 96
Size: 138
Fragments arranged by size:
2660 1712 1443 1254 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2400 2432 7605 7637 2400
Size: 32 5173 32 2408
Fragments arranged by size:
5173 2408 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2502 1316
Size: 1186 6459
XbaI T'CTAG_A
Cuts at: 4981 4981
Size: 7645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3202 4238 3202
Size: 1036 6609
XhoI C'TCGA_G
Cuts at: 1943 3776 1943
Size: 1833 5812
XmaI C'CCGG_G
Cuts at: 1870 2078 2210 3552 4970 1870
Size: 208 132 1342 1418 4545
Fragments arranged by size:
4545 1418 1342 208 132
XmnI GAAnn'nnTTC
Cuts at: 5774 5774
Size: 7645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5453 276
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PsrI SfiI SfoI SrfI SwaI