Restriction map for
pFN21AB8536
(Circular) (Six-base) MAPSORT of: pFN21AB8536.seq Check: 6016 from: 1 to: 7390
pFN21AB8536 7390 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5200 278
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4733 1937
Size: 2796 4594
Acc65I G'GTAC_C
Cuts at: 4711 4711
Size: 7390
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2657 3005 3507 5453 6837 7105 1632
Size: 1025 348 502 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 1025 502 348 268
AflII C'TTAA_G
Cuts at: 820 1017 3577 3781 820
Size: 197 2560 204 4429
Fragments arranged by size:
4429 2560 204 197
AflIII A'CryG_T
Cuts at: 1203 2452 3099 3475 4125 4604 6289 7266 1203
Size: 1249 647 376 650 479 1685 977 1327
Fragments arranged by size:
1685 1327 1249 977 650 647 479 376
AgeI A'CCGG_T
Cuts at: 6221 6221
Size: 7390
AhdI GACnn_n'nnGTC
Cuts at: 6119 6119
Size: 7390
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7390
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3921 3953 3921
Size: 32 7358
AlwNI CAG_nnn'CTG
Cuts at: 2050 2879 3028 4378 5133 6705 2050
Size: 829 149 1350 755 1572 2735
Fragments arranged by size:
2735 1572 1350 829 755 149
ApaI G_GGCC'C
Cuts at: 4527 4527
Size: 7390
ApaLI G'TGCA_C
Cuts at: 5447 6603 5447
Size: 1156 6234
ApoI r'AATT_y
Cuts at: 1493 2206 2551 2736 2848 3746 4441 4667 4699
Size: 713 345 185 112 898 695 226 32
Cuts at: 4699 4925 5077 7186 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 898 713 695 345 226 226
185 152 112 32
AseI AT'TA_AT
Cuts at: 160 1032 3384 5944 160
Size: 872 2352 2560 1606
Fragments arranged by size:
2560 2352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 4715 1870
Size: 73 682 2090 4545
Fragments arranged by size:
4545 2090 682 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3168 3201 3550 3583 3168
Size: 33 349 33 6975
Fragments arranged by size:
6975 349 33 33
BamHI G'GATC_C
Cuts at: 2778 4720 5087 2778
Size: 1942 367 5081
Fragments arranged by size:
5081 1942 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4530 4711 6166 618
Size: 325 217 627 2743 181 1455 1842
Fragments arranged by size:
2743 1842 1455 627 325 217 181
BanII G_rGCy'C
Cuts at: 1391 2626 4392 4527 4709 1391
Size: 1235 1766 135 182 4072
Fragments arranged by size:
4072 1766 1235 182 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3116 4684 928
Size: 971 1217 1568 3634
Fragments arranged by size:
3634 1568 1217 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5581 5615 1903
Size: 34 3644 34 3678
Fragments arranged by size:
3678 3644 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3169 5282 6492 3169
Size: 2113 1210 4067
Fragments arranged by size:
4067 2113 1210
BclI T'GATC_A
Cuts at: 1548 4134 1548
Size: 2586 4804
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2390 6001 136
Size: 107 122 71 631 605 718 3611 1525
Fragments arranged by size:
3611 1525 718 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3840 7385 3840
Size: 3545 3845
BlpI GC'TnA_GC
Cuts at: 4026 4627 4807 4026
Size: 601 180 6609
Fragments arranged by size:
6609 601 180
Bme1580I G_kGCm'C
Cuts at: 1790 2833 4274 4527 5451 6607 1790
Size: 1043 1441 253 924 1156 2573
Fragments arranged by size:
2573 1441 1156 1043 924 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4529 5121 6079
Size: 416 53 83 399 24 3112 592 958
Cuts at: 6079 442
Size: 1753
Fragments arranged by size:
3112 1753 958 592 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4406
Size: 7390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2498 2864 3923 6050 1239
Size: 866 393 366 1059 2127 2579
Fragments arranged by size:
2579 2127 1059 866 393 366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 5515 6380 6678 2297
Size: 3218 865 298 3009
Fragments arranged by size:
3218 3009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3111 3576 6053 882
Size: 617 1612 465 2477 2219
Fragments arranged by size:
2477 2219 1612 617 465
BsaAI yAC'GTr
Cuts at: 493 1204 5181 493
Size: 711 3977 2702
Fragments arranged by size:
3977 2702 711
BsaBI GATnn'nnATC
Cuts at: 3813 7384 3813
Size: 3571 3819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5197 5579 6214 275
Size: 53 83 186 936 3664 382 635 1451
Fragments arranged by size:
3664 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5823 6221 6495 6642 7053 5823
Size: 398 274 147 411 6160
Fragments arranged by size:
6160 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7165
Fragments arranged by size:
7165 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2897 4398 1186
Size: 1711 1501 4178
Fragments arranged by size:
4178 1711 1501
BseYI C'CCAG_C
Cuts at: 4520 6593 7089 4520
Size: 2073 496 4821
Fragments arranged by size:
4821 2073 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3375 4629 1163
Size: 573 1639 1254 3924
Fragments arranged by size:
3924 1639 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4840 5601 6629 1540
Size: 275 186 2839 761 1028 2301
Fragments arranged by size:
2839 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4392 5451 5536 6607 4392
Size: 1059 85 1071 5175
Fragments arranged by size:
5175 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2659 2686 2749 3002 3954 4456 4901 4994 2659
Size: 27 63 253 952 502 445 93 5055
Fragments arranged by size:
5055 952 502 445 253 93 63 27
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2626 2833 4274 4392 4527 4709 5451
Size: 399 836 207 1441 118 135 182 742
Cuts at: 5451 5536 6607 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1441 1071 836 742 399 207 182
135 118 85
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 3259 3856 3876 4137 4659 5279 7009 3259
Size: 597 20 261 522 620 1730 3640
Fragments arranged by size:
3640 1730 620 597 522 261 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 4745 844
Size: 1200 2701 3489
Fragments arranged by size:
3489 2701 1200
BsrBI CCG'CTC
Cuts at: 1394 1823 2983 5277 1394
Size: 429 1160 2294 3507
Fragments arranged by size:
3507 2294 1160 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4482 4649 5885 6059 65
Size: 1183 3234 167 1236 174 1396
Fragments arranged by size:
3234 1396 1236 1183 174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 6034 6221 1700
Size: 110 4224 187 2869
Fragments arranged by size:
4224 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3447 96
Size: 3351 4039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7390
BssSI C'ACGA_G
Cuts at: 3932 5450 6462 3932
Size: 1518 1012 4860
Fragments arranged by size:
4860 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3296 3644 794
Size: 2502 348 4540
Fragments arranged by size:
4540 2502 348
BstBI TT'CG_AA
Cuts at: 2159 6194 2159
Size: 4035 3355
BstEII G'GTnAC_C
Cuts at: 1184 2104 1184
Size: 920 6470
BstXI CCAn_nnnn'nTGG
Cuts at: 2534 3358 2534
Size: 824 6566
BstYI r'GATC_y
Cuts at: 1972 2047 2778 3025 3840 4276 4368 4720 5087
Size: 75 731 247 815 436 92 352 367
Cuts at: 5087 5472 5489 6941 7027 7039 7385 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 815 731 436 385 367 352
346 247 92 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2808 4002 2043
Size: 765 1194 5431
Fragments arranged by size:
5431 1194 765
BtgI C'CryG_G
Cuts at: 513 1065 2070 3438 513
Size: 552 1005 1368 4465
Fragments arranged by size:
4465 1368 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6651
Fragments arranged by size:
6651 683 56
BtsI GCAGTG_nn'
Cuts at: 4906 5700 5720 4906
Size: 794 20 6576
Fragments arranged by size:
6576 794 20
ClaI AT'CG_AT
Cuts at: 1554 2037 1554
Size: 483 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7355
DraI TTT'AAA
Cuts at: 2253 4694 2253
Size: 2441 4949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2479 3417 6397 809
Size: 507 1163 938 2980 1802
Fragments arranged by size:
2980 1802 1163 938 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3220 4116 4837
Size: 54 1425 50 275 18 1390 896 721
Cuts at: 4837 5726 7355 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1390 896 889 721 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4837 1537
Size: 275 3025 4090
Fragments arranged by size:
4090 3025 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3024 3918 5320 1353
Size: 1671 894 1402 3423
Fragments arranged by size:
3423 1671 1402 894
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2963 5977 6351 6497 387
Size: 1064 138 1374 3014 374 146 1280
Fragments arranged by size:
3014 1374 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4390 4390
Size: 7390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2498 2657 2864 3005 3507 3923
Size: 393 473 393 159 207 141 502 416
Cuts at: 3923 5453 6050 6837 7105 1239
Size: 1530 597 787 268 1524
Fragments arranged by size:
1530 1524 787 597 502 473 416 393
393 268 207 159 141
EcoNI CCTnn'n_nnAGG
Cuts at: 3923 3923
Size: 7390
EcoO109I rG'GnC_Cy
Cuts at: 4524 4524
Size: 7390
EcoRI G'AATT_C
Cuts at: 4699 5077 4699
Size: 378 7012
EcoRV GAT'ATC
Cuts at: 3862 4218 3862
Size: 356 7034
FspI TGC'GCA
Cuts at: 5896 5896
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 6537 1678
Size: 4859 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 2397 2429 2576
Size: 32 135 32 585 32 208 32 147
Cuts at: 2576 2608 3143 3172 3175 3204 3858 3890 4249
Size: 32 535 29 3 29 654 32 359
Cuts at: 4249 4281 4377 4409 6031 6063 6105 6137 1373
Size: 32 96 32 1622 32 42 32 2626
Fragments arranged by size:
2626 1622 654 585 535 359 208 147
135 96 42 32 32 32 32 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3559 3718 4734 4980 669
Size: 988 281 1374 247 159 1016 246 3079
Fragments arranged by size:
3079 1374 1016 988 281 247 246 159
HindIII A'AGCT_T
Cuts at: 748 2531 748
Size: 1783 5607
HpaI GTT'AAC
Cuts at: 3718 4980 3718
Size: 1262 6128
KpnI G_GTAC'C
Cuts at: 4715 4715
Size: 7390
MfeI C'AATT_G
Cuts at: 4989 4989
Size: 7390
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3786 6504 6688 1305
Size: 2481 2718 184 2007
Fragments arranged by size:
2718 2481 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4118 10
Size: 54 1425 343 2286 3282
Fragments arranged by size:
3282 2286 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 3443 3474 4130 5348 5707 5866
Size: 648 1525 752 31 656 1218 359 159
Cuts at: 5866 518
Size: 2042
Fragments arranged by size:
2042 1525 1218 752 656 648 359 159
31
MspA1I CmG'CkG
Cuts at: 1606 2012 2318 4613 4806 5483 6631 6876 1606
Size: 406 306 2295 193 677 1148 245 2120
Fragments arranged by size:
2295 2120 1148 677 406 306 245 193
NcoI C'CATG_G
Cuts at: 513 1065 3438 513
Size: 552 2373 4465
Fragments arranged by size:
4465 2373 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7390
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NspI r_CATG'y
Cuts at: 1132 1229 2456 3103 3479 4129 4748 6293 1132
Size: 97 1227 647 376 650 619 1545 2229
Fragments arranged by size:
2229 1545 1227 650 647 619 376 97
PasI CC'CwG_GG
Cuts at: 1348 2821 1348
Size: 1473 5917
PciI A'CATG_T
Cuts at: 2452 3099 3475 4125 6289 2452
Size: 647 376 650 2164 3553
Fragments arranged by size:
3553 2164 650 647 376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3547 4378 1108
Size: 2439 831 4120
Fragments arranged by size:
4120 2439 831
PfoI T'CCnGG_A
Cuts at: 3171 3171
Size: 7390
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5459 5491 6972 7004 5459
Size: 32 1481 32 5845
Fragments arranged by size:
5845 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7390
PsiI TTA'TAA
Cuts at: 4960 4960
Size: 7390
PspOMI G'GGCC_C
Cuts at: 4523 4523
Size: 7390
PstI C_TGCA'G
Cuts at: 830 2055 4352 4742 830
Size: 1225 2297 390 3478
Fragments arranged by size:
3478 2297 1225 390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
PvuII CAG'CTG
Cuts at: 2012 2318 4613 2012
Size: 306 2295 4789
Fragments arranged by size:
4789 2295 306
SacI G_AGCT'C
Cuts at: 4392 4392
Size: 7390
SalI G'TCGA_C
Cuts at: 1936 4732 1936
Size: 2796 4594
SapI GCTCTTCn'nnn_
Cuts at: 3024 3024
Size: 7390
SbfI CC_TGCA'GG
Cuts at: 4742 4742
Size: 7390
ScaI AGT'ACT
Cuts at: 3733 5638 7350 3733
Size: 1905 1712 3773
Fragments arranged by size:
3773 1905 1712
SfcI C'TryA_G
Cuts at: 826 1045 2051 2244 4348 4455 4738 5873 6554
Size: 219 1006 193 2104 107 283 1135 681
Cuts at: 6554 6745 826
Size: 191 1471
Fragments arranged by size:
2104 1471 1135 1006 681 283 219 193
191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7390
SmaI CCC'GGG
Cuts at: 1872 4717 1872
Size: 2845 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 3577 3781 5494 6395 6657
Size: 197 926 333 1301 204 1713 901 262
Cuts at: 6657 820
Size: 1553
Fragments arranged by size:
1713 1553 1301 926 901 333 262 204
197
SnaBI TAC'GTA
Cuts at: 493 5181 493
Size: 4688 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7390
SphI G_CATG'C
Cuts at: 1132 4748 1132
Size: 3616 3774
SspI AAT'ATT
Cuts at: 5 52 3085 5314 5
Size: 47 3033 2229 2081
Fragments arranged by size:
3033 2229 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7390
StyI C'CwwG_G
Cuts at: 513 1065 3438 3771 4829 513
Size: 552 2373 333 1058 3074
Fragments arranged by size:
3074 2373 1058 552 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 4548 5417 5434 5587 5772 6217
Size: 1255 2397 571 869 17 153 185 445
Cuts at: 6217 325
Size: 1498
Fragments arranged by size:
2397 1498 1255 869 571 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3039 3447 3731
Size: 274 80 33 51 1443 1062 408 284
Cuts at: 3731 5636 7348 96
Size: 1905 1712 138
Fragments arranged by size:
1905 1712 1443 1062 408 284 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 3353 3385 7350 7382 2763
Size: 32 558 32 3965 32 2771
Fragments arranged by size:
3965 2771 558 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4412 1316
Size: 3096 4294
XbaI T'CTAG_A
Cuts at: 4726 4726
Size: 7390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4517 4542 4517
Size: 25 7365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7390
XmaI C'CCGG_G
Cuts at: 1870 4715 1870
Size: 2845 4545
XmnI GAAnn'nnTTC
Cuts at: 2736 5519 2736
Size: 2783 4607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5198 276
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI