Restriction map for
pFN21AB8531
(Circular) (Six-base) MAPSORT of: pFN21AB8531.seq Check: 5603 from: 1 to: 7594
pFN21AB8531 7594 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5404 278
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4937 1937
Size: 3000 4594
Acc65I G'GTAC_C
Cuts at: 4915 4915
Size: 7594
AclI AA'CG_TT
Cuts at: 1563 3823 5721 6094 1563
Size: 2260 1898 373 3063
Fragments arranged by size:
3063 2260 1898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 2088 2754 3371 3599 4367 5657 7041
Size: 400 56 666 617 228 768 1290 1384
Cuts at: 7041 7309 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1290 768 666 617 400 268
228 56
AfeI AGC'GCT
Cuts at: 4480 4480
Size: 7594
AflII C'TTAA_G
Cuts at: 820 1017 3623 820
Size: 197 2606 4791
Fragments arranged by size:
4791 2606 197
AflIII A'CryG_T
Cuts at: 1203 2575 6493 7470 1203
Size: 1372 3918 977 1327
Fragments arranged by size:
3918 1372 1327 977
AgeI A'CCGG_T
Cuts at: 6425 6425
Size: 7594
AhdI GACnn_n'nnGTC
Cuts at: 6323 6323
Size: 7594
AleI CACnn'nnGTG
Cuts at: 1166 2120 4535 4815 1166
Size: 954 2415 280 3945
Fragments arranged by size:
3945 2415 954 280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7594
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4101 4133 4101
Size: 32 7562
AlwNI CAG_nnn'CTG
Cuts at: 4423 5337 6909 4423
Size: 914 1572 5108
Fragments arranged by size:
5108 1572 914
ApaLI G'TGCA_C
Cuts at: 2506 4538 5651 6807 2506
Size: 2032 1113 1156 3293
Fragments arranged by size:
3293 2032 1156 1113
ApoI r'AATT_y
Cuts at: 1493 2377 2384 3238 4903 5129 5281 7390 1493
Size: 884 7 854 1665 226 152 2109 1697
Fragments arranged by size:
2109 1697 1665 884 854 226 152 7
AseI AT'TA_AT
Cuts at: 160 1032 2634 6148 160
Size: 872 1602 3514 1606
Fragments arranged by size:
3514 1606 1602 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
AvaI C'yCGr_G
Cuts at: 1870 1943 4781 4919 1870
Size: 73 2838 138 4545
Fragments arranged by size:
4545 2838 138 73
BamHI G'GATC_C
Cuts at: 4924 5291 4924
Size: 367 7227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 4915 6370 618
Size: 325 217 627 1337 1791 1455 1842
Fragments arranged by size:
1842 1791 1455 1337 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3099 4913 1391
Size: 1708 1814 4072
Fragments arranged by size:
4072 1814 1708
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 2282 2481 3359 3540 928
Size: 971 219 164 199 878 181 4982
Fragments arranged by size:
4982 971 878 219 199 181 164
BbvCI CC'TCA_GC
Cuts at: 2811 2811
Size: 7594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5785 5819 1903
Size: 34 3848 34 3678
Fragments arranged by size:
3848 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2539 5486 6696 2539
Size: 2947 1210 3437
Fragments arranged by size:
3437 2947 1210
BclI T'GATC_A
Cuts at: 1548 3511 1548
Size: 1963 5631
BfrBI ATG'CAT
Cuts at: 2144 4667 2144
Size: 2523 5071
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6205 136
Size: 107 122 71 631 605 4533 1525
Fragments arranged by size:
4533 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4567 7589 4567
Size: 3022 4572
BlpI GC'TnA_GC
Cuts at: 3420 3609 5011 3420
Size: 189 1402 6003
Fragments arranged by size:
6003 1402 189
Bme1580I G_kGCm'C
Cuts at: 1790 2510 4542 5655 6811 1790
Size: 720 2032 1113 1156 2573
Fragments arranged by size:
2573 2032 1156 1113 720
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5325 6283 442
Size: 416 53 83 399 24 3908 958 1753
Fragments arranged by size:
3908 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 6254 1239
Size: 1509 3506 2579
Fragments arranged by size:
3506 2579 1509
Bpu10I CC'TnA_GC
Cuts at: 2811 2811
Size: 7594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2160 5719 6584 6882 2160
Size: 3559 865 298 2872
Fragments arranged by size:
3559 2872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6257 882
Size: 617 4758 2219
Fragments arranged by size:
4758 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4556 5385 493
Size: 711 3352 829 2702
Fragments arranged by size:
3352 2702 829 711
BsaBI GATnn'nnATC
Cuts at: 7588 7588
Size: 7594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5401 5783 6418 275
Size: 53 83 186 936 3868 382 635 1451
Fragments arranged by size:
3868 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4469 6027 6425 6699 6846 7257 4469
Size: 1558 398 274 147 411 4806
Fragments arranged by size:
4806 1558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2605 2635 1178
Size: 30 165 30 1202 30 6137
Fragments arranged by size:
6137 1202 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3389 4845 4893 1186
Size: 2203 1456 48 3887
Fragments arranged by size:
3887 2203 1456 48
BseYI C'CCAG_C
Cuts at: 6797 7293 6797
Size: 496 7098
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4740 1163
Size: 573 3004 4017
Fragments arranged by size:
4017 3004 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 5044 5805 6833 1540
Size: 275 186 120 2923 761 1028 2301
Fragments arranged by size:
2923 2301 1028 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 2510 3099 4033 4065 4542 5655 5740 6811 2510
Size: 589 934 32 477 1113 85 1071 3293
Fragments arranged by size:
3293 1113 1071 934 589 477 85 32
BsmI GAATG_Cn'
Cuts at: 4750 5105 5198 4750
Size: 355 93 7146
Fragments arranged by size:
7146 355 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3113 3113
Size: 7594
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2510 3099 4033 4065 4542 4913 5655
Size: 399 720 589 934 32 477 371 742
Cuts at: 5655 5740 6811 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 934 742 720 589 477 399
371 85 32
BspEI T'CCGG_A
Cuts at: 7257 7257
Size: 7594
BspHI T'CATG_A
Cuts at: 2302 2470 5483 7213 2302
Size: 168 3013 1730 2683
Fragments arranged by size:
3013 2683 1730 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4407 4949 844
Size: 3563 542 3489
Fragments arranged by size:
3563 3489 542
BsrBI CCG'CTC
Cuts at: 1394 1823 5481 1394
Size: 429 3658 3507
Fragments arranged by size:
3658 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6089 6263 65
Size: 1183 4841 174 1396
Fragments arranged by size:
4841 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6238 6425 1700
Size: 110 4428 187 2869
Fragments arranged by size:
4428 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7594
BssSI C'ACGA_G
Cuts at: 4671 5654 6666 4671
Size: 983 1012 5599
Fragments arranged by size:
5599 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4763 794
Size: 3969 3625
BstBI TT'CG_AA
Cuts at: 6398 6398
Size: 7594
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7594
BstYI r'GATC_y
Cuts at: 1972 3060 4116 4567 4924 5291 5676 5693 7145
Size: 1088 1056 451 357 367 385 17 1452
Cuts at: 7145 7231 7243 7589 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1088 1056 451 385 367 357
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3735 3735
Size: 7594
BtgI C'CryG_G
Cuts at: 513 1065 2004 2077 2367 3813 513
Size: 552 939 73 290 1446 4294
Fragments arranged by size:
4294 1446 939 552 290 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 487
Size: 683 56 851 6004
Fragments arranged by size:
6004 851 683 56
BtsI GCAGTG_nn'
Cuts at: 4872 5110 5904 5924 4872
Size: 238 794 20 6542
Fragments arranged by size:
6542 794 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7594
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7559
DraI TTT'AAA
Cuts at: 4898 4898
Size: 7594
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6601 809
Size: 507 5285 1802
Fragments arranged by size:
5285 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2959 4737
Size: 54 1425 50 275 18 186 943 1778
Cuts at: 4737 5041 5930 7559 8
Size: 304 889 1629 43
Fragments arranged by size:
1778 1629 1425 943 889 304 275 186
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5041 1537
Size: 275 3229 4090
Fragments arranged by size:
4090 3229 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3753 4356 4738 5524 1353
Size: 2400 603 382 786 3423
Fragments arranged by size:
3423 2400 786 603 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6181 6555 6701 387
Size: 1064 138 435 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3097 3097
Size: 7594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2088 2748 2754 3371 3599 4367
Size: 393 400 56 660 6 617 228 768
Cuts at: 4367 5657 6254 7041 7309 1239
Size: 1290 597 787 268 1524
Fragments arranged by size:
1524 1290 787 768 660 617 597 400
393 268 228 56 6
EcoO109I rG'GnC_Cy
Cuts at: 2434 3484 4420 2434
Size: 1050 936 5608
Fragments arranged by size:
5608 1050 936
EcoRI G'AATT_C
Cuts at: 4903 5281 4903
Size: 378 7216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 4081 4205 2240
Size: 1841 124 5629
Fragments arranged by size:
5629 1841 124
FspI TGC'GCA
Cuts at: 4403 6100 4403
Size: 1697 5897
HaeII r_GCGC'y
Cuts at: 1678 4482 6741 1678
Size: 2804 2259 2531
Fragments arranged by size:
2804 2531 2259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3503 3535 6235 6267 6309
Size: 32 135 32 1931 32 2700 32 42
Cuts at: 6309 6341 1373
Size: 32 2626
Fragments arranged by size:
2700 2626 1931 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3335 4938 5184 669
Size: 988 281 1071 326 1603 246 3079
Fragments arranged by size:
3079 1603 1071 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 3168 4629 748
Size: 2420 1461 3713
Fragments arranged by size:
3713 2420 1461
HpaI GTT'AAC
Cuts at: 3009 5184 3009
Size: 2175 5419
KpnI G_GTAC'C
Cuts at: 4919 4919
Size: 7594
MfeI C'AATT_G
Cuts at: 3222 5193 3222
Size: 1971 5623
MluI A'CGCG_T
Cuts at: 7470 7470
Size: 7594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2592 3345 3963 4572 4829 6708 6892 1305
Size: 1287 753 618 609 257 1879 184 2007
Fragments arranged by size:
2007 1879 1287 753 618 609 257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2961 4739 10
Size: 54 1425 343 1129 1778 2865
Fragments arranged by size:
2865 1778 1425 1129 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2120 4535 4815 5552 5911 6070
Size: 648 867 87 2415 280 737 359 159
Cuts at: 6070 518
Size: 2042
Fragments arranged by size:
2415 2042 867 737 648 359 280 159
87
MspA1I CmG'CkG
Cuts at: 1606 2079 3659 4575 4616 5010 5687 6835 7080
Size: 473 1580 916 41 394 677 1148 245
Cuts at: 7080 1606
Size: 2120
Fragments arranged by size:
2120 1580 1148 916 677 473 394 245
41
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 3813 513
Size: 552 939 363 1446 4294
Fragments arranged by size:
4294 1446 939 552 363
NdeI CA'TA_TG
Cuts at: 387 2254 2824 387
Size: 1867 570 5157
Fragments arranged by size:
5157 1867 570
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7594
NotI GC'GGCC_GC
Cuts at: 5041 5041
Size: 7594
NsiI A_TGCA'T
Cuts at: 2146 4669 2146
Size: 2523 5071
NspI r_CATG'y
Cuts at: 1132 1229 2579 3900 4952 6497 1132
Size: 97 1350 1321 1052 1545 2229
Fragments arranged by size:
2229 1545 1350 1321 1052 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7594
PciI A'CATG_T
Cuts at: 2575 6493 2575
Size: 3918 3676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4051 4861 1108
Size: 2943 810 3841
Fragments arranged by size:
3841 2943 810
PmeI GTTT'AAAC
Cuts at: 4898 4898
Size: 7594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 7176 7208 5663
Size: 32 1481 32 6049
Fragments arranged by size:
6049 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 4420 2434
Size: 1986 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7594
PsiI TTA'TAA
Cuts at: 5164 5164
Size: 7594
PstI C_TGCA'G
Cuts at: 830 3016 4946 830
Size: 2186 1930 3478
Fragments arranged by size:
3478 2186 1930
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7594
PvuII CAG'CTG
Cuts at: 3659 4616 3659
Size: 957 6637
SacI G_AGCT'C
Cuts at: 3099 3099
Size: 7594
SacII CC_GC'GG
Cuts at: 2080 2080
Size: 7594
SalI G'TCGA_C
Cuts at: 1936 4936 1936
Size: 3000 4594
SbfI CC_TGCA'GG
Cuts at: 4946 4946
Size: 7594
ScaI AGT'ACT
Cuts at: 5842 7554 5842
Size: 1712 5882
SfcI C'TryA_G
Cuts at: 826 1045 2884 3012 4611 4942 6077 6758 6949
Size: 219 1839 128 1599 331 1135 681 191
Cuts at: 6949 826
Size: 1471
Fragments arranged by size:
1839 1599 1471 1135 681 331 219 191
128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7594
SmaI CCC'GGG
Cuts at: 1872 4783 4921 1872
Size: 2911 138 4545
Fragments arranged by size:
4545 2911 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 3623 5698 6599 6861 820
Size: 197 926 232 1448 2075 901 262 1553
Fragments arranged by size:
2075 1553 1448 926 901 262 232 197
SnaBI TAC'GTA
Cuts at: 493 5385 493
Size: 4892 2702
SpeI A'CTAG_T
Cuts at: 152 4015 152
Size: 3863 3731
SphI G_CATG'C
Cuts at: 1132 4952 1132
Size: 3820 3774
SspI AAT'ATT
Cuts at: 5 52 2247 5518 5
Size: 47 2195 3271 2081
Fragments arranged by size:
3271 2195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7594
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 3813 5033 513
Size: 552 939 363 1446 1220 3074
Fragments arranged by size:
3074 1446 1220 939 552 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4827 5621 5638 5791 5976 6421 325
Size: 1255 3247 794 17 153 185 445 1498
Fragments arranged by size:
3247 1498 1255 794 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2221 2819
Size: 274 80 33 51 1443 111 133 598
Cuts at: 2819 5840 7552 96
Size: 3021 1712 138
Fragments arranged by size:
3021 1712 1443 598 274 138 133 111
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3693 3725 7554 7586 3693
Size: 32 3829 32 3701
Fragments arranged by size:
3829 3701 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2117 1316
Size: 801 6793
XbaI T'CTAG_A
Cuts at: 4930 4930
Size: 7594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 7594
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7594
XmaI C'CCGG_G
Cuts at: 1870 4781 4919 1870
Size: 2911 138 4545
Fragments arranged by size:
4545 2911 138
XmnI GAAnn'nnTTC
Cuts at: 3951 5723 3951
Size: 1772 5822
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5402 276
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI
SrfI SwaI