Restriction map for
pFN21AB8524
(Circular) (Six-base) MAPSORT of: pFN21AB8524.seq Check: 8459 from: 1 to: 7216
pFN21AB8524 7216 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2170
Size: 7216
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5026 278
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 4559 1937
Size: 328 2294 4594
Fragments arranged by size:
4594 2294 328
Acc65I G'GTAC_C
Cuts at: 3319 4537 3319
Size: 1218 5998
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 3180 3362 3510 5279 6663 6931 1632
Size: 506 1042 182 148 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 1042 506 268 182 148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7019
AflIII A'CryG_T
Cuts at: 1203 6115 7092 1203
Size: 4912 977 1327
Fragments arranged by size:
4912 1327 977
AgeI A'CCGG_T
Cuts at: 6047 6047
Size: 7216
AhdI GACnn_n'nnGTC
Cuts at: 5945 5945
Size: 7216
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 7184
AlwNI CAG_nnn'CTG
Cuts at: 2068 3340 4253 4959 6531 2068
Size: 1272 913 706 1572 2753
Fragments arranged by size:
2753 1572 1272 913 706
ApaI G_GGCC'C
Cuts at: 2922 2922
Size: 7216
ApaLI G'TGCA_C
Cuts at: 5273 6429 5273
Size: 1156 6060
ApoI r'AATT_y
Cuts at: 1493 3394 4525 4751 4903 7012 1493
Size: 1901 1131 226 152 2109 1697
Fragments arranged by size:
2109 1901 1697 1131 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 4219 4541 1870
Size: 73 1140 1136 322 4545
Fragments arranged by size:
4545 1140 1136 322 73
BamHI G'GATC_C
Cuts at: 2169 4546 4913 2169
Size: 2377 367 4472
Fragments arranged by size:
4472 2377 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2621 2827 3319 4537
Size: 325 217 627 231 603 206 492 1218
Cuts at: 4537 5992 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1218 627 603 492 325 231
217 206
BanII G_rGCy'C
Cuts at: 1391 2482 2802 2885 2922 3738 3988 4535 1391
Size: 1091 320 83 37 816 250 547 4072
Fragments arranged by size:
4072 1091 816 547 320 250 83 37
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 7216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3237 3285 4469 928
Size: 971 1338 48 1184 3675
Fragments arranged by size:
3675 1338 1184 971 48
BbvCI CC'TCA_GC
Cuts at: 2906 4130 2906
Size: 1224 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4346 4380 5407 5441 1903
Size: 34 2409 34 1027 34 3678
Fragments arranged by size:
3678 2409 1027 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5108 6318 5108
Size: 1210 6006
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7216
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 2619 2717
Size: 107 122 71 631 605 473 474 98
Cuts at: 2717 5827 136
Size: 3110 1525
Fragments arranged by size:
3110 1525 631 605 474 473 122 107
98 71
BglII A'GATC_T
Cuts at: 7211 7211
Size: 7216
BlpI GC'TnA_GC
Cuts at: 4633 4633
Size: 7216
Bme1580I G_kGCm'C
Cuts at: 1790 2532 2832 2922 4420 5277 6433 1790
Size: 742 300 90 1498 857 1156 2573
Fragments arranged by size:
2573 1498 1156 857 742 300 90
BmgBI CAC'GTC
Cuts at: 1318 3856 1318
Size: 2538 4678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 4947 5905
Size: 416 53 83 399 24 1499 2031 958
Cuts at: 5905 442
Size: 1753
Fragments arranged by size:
2031 1753 1499 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2451 2604 2451
Size: 153 7063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2468 3083 3317 3746 5876 1239
Size: 832 397 615 234 429 2130 2579
Fragments arranged by size:
2579 2130 832 615 429 397 234
Bpu10I CC'TnA_GC
Cuts at: 2906 4130 2906
Size: 1224 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3182 3280 3401 5341 6206 6504 2446
Size: 736 98 121 1940 865 298 3158
Fragments arranged by size:
3158 1940 865 736 298 121 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5879 882
Size: 617 4380 2219
Fragments arranged by size:
4380 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2730 5007 493
Size: 711 1526 2277 2702
Fragments arranged by size:
2702 2277 1526 711
BsaBI GATnn'nnATC
Cuts at: 7210 7210
Size: 7216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 5023 5405 6040
Size: 53 83 186 936 486 3004 382 635
Cuts at: 6040 275
Size: 1451
Fragments arranged by size:
3004 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2043 3774 5649 6047 6321 6468 6879 2043
Size: 1731 1875 398 274 147 411 2380
Fragments arranged by size:
2380 1875 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3887 3917 1178
Size: 30 165 30 2484 30 4477
Fragments arranged by size:
4477 2484 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2455 2962 1186
Size: 1269 507 5440
Fragments arranged by size:
5440 1269 507
BseYI C'CCAG_C
Cuts at: 2023 2370 2525 2915 3030 3034 6419 6915 2023
Size: 347 155 390 115 4 3385 496 2324
Fragments arranged by size:
3385 2324 496 390 347 155 115 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2522 4371 1163
Size: 573 468 318 1849 4008
Fragments arranged by size:
4008 1849 573 468 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 2979 4005 4666 5427 6455
Size: 275 186 139 839 1026 661 761 1028
Cuts at: 6455 1540
Size: 2301
Fragments arranged by size:
2301 1028 1026 839 761 661 275 186
139
BsiHKAI G_wGCw'C
Cuts at: 2885 3738 3988 5277 5362 6433 2885
Size: 853 250 1289 85 1071 3668
Fragments arranged by size:
3668 1289 1071 853 250 85
BsmI GAATG_Cn'
Cuts at: 3046 3699 3907 4303 4727 4820 3046
Size: 653 208 396 424 93 5442
Fragments arranged by size:
5442 653 424 396 208 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2482 2532 2802 2832 2885 2922 3738
Size: 399 692 50 270 30 53 37 816
Cuts at: 3738 3988 4420 4535 5277 5362 6433 1391
Size: 250 432 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 816 742 692 432 399 270
250 115 85 53 50 37 30
BspEI T'CCGG_A
Cuts at: 2043 6879 2043
Size: 4836 2380
BspHI T'CATG_A
Cuts at: 3426 5105 6835 3426
Size: 1679 1730 3807
Fragments arranged by size:
3807 1730 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 2803 4571 844
Size: 1326 633 1768 3489
Fragments arranged by size:
3489 1768 1326 633
BsrBI CCG'CTC
Cuts at: 1394 1823 5103 1394
Size: 429 3280 3507
Fragments arranged by size:
3507 3280 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3440 3651 5711 5885 65
Size: 1183 2192 211 2060 174 1396
Fragments arranged by size:
2192 2060 1396 1183 211 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5860 6047 1700
Size: 110 4050 187 2869
Fragments arranged by size:
4050 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7216
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7216
BssSI C'ACGA_G
Cuts at: 3140 5276 6288 3140
Size: 2136 1012 4068
Fragments arranged by size:
4068 2136 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 4358 794
Size: 1742 1822 3652
Fragments arranged by size:
3652 1822 1742
BstBI TT'CG_AA
Cuts at: 6020 6020
Size: 7216
BstEII G'GTnAC_C
Cuts at: 1184 4094 1184
Size: 2910 4306
BstXI CCAn_nnnn'nTGG
Cuts at: 3188 3358 3892 3188
Size: 170 534 6512
Fragments arranged by size:
6512 534 170
BstYI r'GATC_y
Cuts at: 1972 2064 2169 4546 4913 5298 5315 6767 6853
Size: 92 105 2377 367 385 17 1452 86
Cuts at: 6853 6865 7211 1972
Size: 12 346 1977
Fragments arranged by size:
2377 1977 1452 385 367 346 105 92
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3979 3979
Size: 7216
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 2217 2409 2931 3681 513
Size: 552 939 136 77 192 522 750 4048
Fragments arranged by size:
4048 939 750 552 522 192 136 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3611 4371 487
Size: 683 56 2385 760 3332
Fragments arranged by size:
3332 2385 760 683 56
BtsI GCAGTG_nn'
Cuts at: 2254 3078 4732 5526 5546 2254
Size: 824 1654 794 20 3924
Fragments arranged by size:
3924 1654 824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3629 6223 809
Size: 507 2313 2594 1802
Fragments arranged by size:
2594 2313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2146 2968
Size: 54 1425 50 275 18 307 9 822
Cuts at: 2968 2976 4002 4341 4663 5552 7181 8
Size: 8 1026 339 322 889 1629 43
Fragments arranged by size:
1629 1425 1026 889 822 339 322 307
275 54 50 43 18 9 8
EagI C'GGCC_G
Cuts at: 1537 1812 2137 2976 4002 4663 1537
Size: 275 325 839 1026 661 4090
Fragments arranged by size:
4090 1026 839 661 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2371 3164 5146 1353
Size: 1018 793 1982 3423
Fragments arranged by size:
3423 1982 1018 793
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5803 6177 6323 387
Size: 1064 138 4214 374 146 1280
Fragments arranged by size:
4214 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2883 3736 3986 2883
Size: 853 250 6113
Fragments arranged by size:
6113 853 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2138 2468 3083 3180 3317 3362
Size: 393 439 67 330 615 97 137 45
Cuts at: 3362 3510 3746 5279 5876 6663 6931 1239
Size: 148 236 1533 597 787 268 1524
Fragments arranged by size:
1533 1524 787 615 597 439 393 330
268 236 148 137 97 67 45
EcoO109I rG'GnC_Cy
Cuts at: 2421 2865 3255 2421
Size: 444 390 6382
Fragments arranged by size:
6382 444 390
EcoRI G'AATT_C
Cuts at: 4525 4903 4525
Size: 378 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3461 3461
Size: 7216
FspI TGC'GCA
Cuts at: 5722 5722
Size: 7216
HaeII r_GCGC'y
Cuts at: 1678 2022 2099 2641 3582 6363 1678
Size: 344 77 542 941 2781 2531
Fragments arranged by size:
2781 2531 941 542 344 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5857 5889 5931 5963 1373
Size: 32 135 32 4285 32 42 32 2626
Fragments arranged by size:
4285 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 4477 4560 4806 669
Size: 988 281 1240 1299 83 246 3079
Fragments arranged by size:
3079 1299 1240 988 281 246 83
HindIII A'AGCT_T
Cuts at: 748 3200 748
Size: 2452 4764
HpaI GTT'AAC
Cuts at: 3178 4806 3178
Size: 1628 5588
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 7216
KpnI G_GTAC'C
Cuts at: 3323 4541 3323
Size: 1218 5998
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 4395 6330 6514 1305
Size: 973 2117 1935 184 2007
Fragments arranged by size:
2117 2007 1935 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4343 10
Size: 54 1425 343 2511 2883
Fragments arranged by size:
2883 2511 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3053 4092 5174 5533 5692 518
Size: 648 1887 1039 1082 359 159 2042
Fragments arranged by size:
2042 1887 1082 1039 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2136 2142 2824 2943 3034 3724 4632
Size: 421 109 6 682 119 91 690 908
Cuts at: 4632 5309 6457 6702 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 908 690 682 677 421 245
119 109 91 6
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 7216
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5725
Fragments arranged by size:
5725 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 2137 2976 4663 2137
Size: 839 1687 4690
Fragments arranged by size:
4690 1687 839
NspI r_CATG'y
Cuts at: 1132 1229 2335 3100 4091 4574 6119 1132
Size: 97 1106 765 991 483 1545 2229
Fragments arranged by size:
2229 1545 1106 991 765 483 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7216
PciI A'CATG_T
Cuts at: 6115 6115
Size: 7216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7216
PfoI T'CCnGG_A
Cuts at: 2172 3107 4142 2172
Size: 935 1035 5246
Fragments arranged by size:
5246 1035 935
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 6798 6830 5285
Size: 32 1481 32 5671
Fragments arranged by size:
5671 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2865 2421
Size: 444 6772
PshAI GACnn'nnGTC
Cuts at: 1613 4043 1613
Size: 2430 4786
PsiI TTA'TAA
Cuts at: 3920 4786 3920
Size: 866 6350
PspOMI G'GGCC_C
Cuts at: 2918 2918
Size: 7216
PspXI vC'TCGA_Gb
Cuts at: 3083 3083
Size: 7216
PstI C_TGCA'G
Cuts at: 830 2838 3124 3210 3993 4068 4161 4568 830
Size: 2008 286 86 783 75 93 407 3478
Fragments arranged by size:
3478 2008 783 407 286 93 86 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
PvuII CAG'CTG
Cuts at: 2027 3034 3724 2027
Size: 1007 690 5519
Fragments arranged by size:
5519 1007 690
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 7216
SacI G_AGCT'C
Cuts at: 2885 3738 3988 2885
Size: 853 250 6113
Fragments arranged by size:
6113 853 250
SacII CC_GC'GG
Cuts at: 2143 2143
Size: 7216
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SbfI CC_TGCA'GG
Cuts at: 4568 4568
Size: 7216
ScaI AGT'ACT
Cuts at: 4225 5464 7176 4225
Size: 1239 1712 4265
Fragments arranged by size:
4265 1712 1239
SfcI C'TryA_G
Cuts at: 826 1045 2834 3120 3206 3989 4064 4157 4245
Size: 219 1789 286 86 783 75 93 88
Cuts at: 4245 4564 5699 6380 6571 826
Size: 319 1135 681 191 1471
Fragments arranged by size:
1789 1471 1135 783 681 319 286 219
191 93 88 86 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 2145 2145
Size: 7216
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 7216
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7216
SmaI CCC'GGG
Cuts at: 1872 4543 1872
Size: 2671 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 3083 3197 3259 3416 5320
Size: 197 926 482 658 114 62 157 1904
Cuts at: 5320 6221 6483 820
Size: 901 262 1553
Fragments arranged by size:
1904 1553 926 901 658 482 262 197
157 114 62
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 4574 1132
Size: 3442 3774
SspI AAT'ATT
Cuts at: 5 52 3749 5140 5
Size: 47 3697 1391 2081
Fragments arranged by size:
3697 2081 1391 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7216
StyI C'CwwG_G
Cuts at: 513 1065 2004 3885 4655 513
Size: 552 939 1881 770 3074
Fragments arranged by size:
3074 1881 939 770 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3471 5243 5260 5413 5598 6043 325
Size: 1255 1891 1772 17 153 185 445 1498
Fragments arranged by size:
1891 1772 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 2505 4223
Size: 274 80 33 51 1443 414 114 1718
Cuts at: 4223 5462 7174 96
Size: 1239 1712 138
Fragments arranged by size:
1718 1712 1443 1239 414 274 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 7176 7208 3887
Size: 32 3257 32 3895
Fragments arranged by size:
3895 3257 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4214 1316
Size: 2898 4318
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2755 2929 4055 2119
Size: 636 174 1126 5280
Fragments arranged by size:
5280 1126 636 174
XhoI C'TCGA_G
Cuts at: 1943 3083 1943
Size: 1140 6076
XmaI C'CCGG_G
Cuts at: 1870 4541 1870
Size: 2671 4545
XmnI GAAnn'nnTTC
Cuts at: 3234 5345 3234
Size: 2111 5105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5024 276
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PsrI SanDI SapI SexAI SrfI
SwaI