Restriction map for
pFN21AB8523
(Circular) (Six-base) MAPSORT of: pFN21AB8523.seq Check: 2614 from: 1 to: 7516
pFN21AB8523 7516 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2781 2781
Size: 7516
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4329 5326 278
Size: 53 83 186 936 2793 997 2468
Fragments arranged by size:
2793 2468 997 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 4628 4859 1937
Size: 664 2027 231 4594
Fragments arranged by size:
4594 2027 664 231
Acc65I G'GTAC_C
Cuts at: 4837 4837
Size: 7516
AclI AA'CG_TT
Cuts at: 1563 5643 6016 1563
Size: 4080 373 3063
Fragments arranged by size:
4080 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3975 4644 5579 6963 7231 1632
Size: 2343 669 935 1384 268 1917
Fragments arranged by size:
2343 1917 1384 935 669 268
AfeI AGC'GCT
Cuts at: 4760 4760
Size: 7516
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7319
AflIII A'CryG_T
Cuts at: 1203 2724 3544 3569 3768 4224 6415 7392 1203
Size: 1521 820 25 199 456 2191 977 1327
Fragments arranged by size:
2191 1521 1327 977 820 456 199 25
AgeI A'CCGG_T
Cuts at: 6347 6347
Size: 7516
AhdI GACnn_n'nnGTC
Cuts at: 2051 6245 2051
Size: 4194 3322
AleI CACnn'nnGTG
Cuts at: 1166 2288 2871 2994 3129 3609 3744 3867 4277
Size: 1122 583 123 135 480 135 123 410
Cuts at: 4277 1166
Size: 4405
Fragments arranged by size:
4405 1122 583 480 410 135 135 123
123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7516
AlwNI CAG_nnn'CTG
Cuts at: 2848 5259 6831 2848
Size: 2411 1572 3533
Fragments arranged by size:
3533 2411 1572
ApaI G_GGCC'C
Cuts at: 2467 2467
Size: 7516
ApaLI G'TGCA_C
Cuts at: 2713 3079 3222 3345 5573 6729 2713
Size: 366 143 123 2228 1156 3500
Fragments arranged by size:
3500 2228 1156 366 143 123
ApoI r'AATT_y
Cuts at: 1493 3883 4012 4825 5051 5203 7312 1493
Size: 2390 129 813 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 813 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 6070 160
Size: 872 5038 1606
Fragments arranged by size:
5038 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 3453 4080 4841 1870
Size: 73 343 1167 627 761 4545
Fragments arranged by size:
4545 1167 761 627 343 73
BamHI G'GATC_C
Cuts at: 3989 4846 5213 3989
Size: 857 367 6292
Fragments arranged by size:
6292 857 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2402 4279 4837 6292 618
Size: 325 217 627 615 1877 558 1455 1842
Fragments arranged by size:
1877 1842 1455 627 615 558 325 217
BanII G_rGCy'C
Cuts at: 1391 2467 2637 2903 4199 4318 4431 4835 1391
Size: 1076 170 266 1296 119 113 404 4072
Fragments arranged by size:
4072 1296 1076 404 266 170 119 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2644 3572 3940 4527 4540 4617 928
Size: 971 745 928 368 587 13 77 3827
Fragments arranged by size:
3827 971 928 745 587 368 77 13
BbvCI CC'TCA_GC
Cuts at: 2940 3801 3924 2940
Size: 861 123 6532
Fragments arranged by size:
6532 861 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2528 2562 5707 5741 1903
Size: 34 591 34 3145 34 3678
Fragments arranged by size:
3678 3145 591 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5408 6618 5408
Size: 1210 6306
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2543 3171 3294
Size: 107 122 71 631 605 871 628 123
Cuts at: 3294 6127 136
Size: 2833 1525
Fragments arranged by size:
2833 1525 871 631 628 605 123 122
107 71
BglII A'GATC_T
Cuts at: 7511 7511
Size: 7516
BlpI GC'TnA_GC
Cuts at: 4933 4933
Size: 7516
Bme1580I G_kGCm'C
Cuts at: 1790 2405 2467 2717 2755 3083 3226 3349 4284
Size: 615 62 250 38 328 143 123 935
Cuts at: 4284 5577 6733 1790
Size: 1293 1156 2573
Fragments arranged by size:
2573 1293 1156 935 615 328 250 143
123 62 38
BmgBI CAC'GTC
Cuts at: 1318 3570 1318
Size: 2252 5264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2421 4215 5247
Size: 416 53 83 399 24 1004 1794 1032
Cuts at: 5247 6205 442
Size: 958 1753
Fragments arranged by size:
1794 1753 1032 1004 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7516
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4438 4438
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 4079 4530 6176 1239
Size: 926 1914 451 1646 2579
Fragments arranged by size:
2579 1914 1646 926 451
Bpu10I CC'TnA_GC
Cuts at: 2550 2940 3801 3924 4317 2550
Size: 390 861 123 393 5749
Fragments arranged by size:
5749 861 393 390 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 3425 4478 5641 6506 6804 2321
Size: 1104 1053 1163 865 298 3033
Fragments arranged by size:
3033 1163 1104 1053 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2512 6179 882
Size: 617 1013 3667 2219
Fragments arranged by size:
3667 2219 1013 617
BsaAI yAC'GTr
Cuts at: 493 1204 2087 3547 3771 5307 493
Size: 711 883 1460 224 1536 2702
Fragments arranged by size:
2702 1536 1460 883 711 224
BsaBI GATnn'nnATC
Cuts at: 7510 7510
Size: 7516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4326 5323 5705 6340
Size: 53 83 186 936 2793 997 382 635
Cuts at: 6340 275
Size: 1451
Fragments arranged by size:
2793 1451 997 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5949 6347 6621 6768 7179 5949
Size: 398 274 147 411 6286
Fragments arranged by size:
6286 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3534 3564 1178
Size: 30 165 30 2131 30 5130
Fragments arranged by size:
5130 2131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 3281 4415 1186
Size: 862 1233 1134 4287
Fragments arranged by size:
4287 1233 1134 862
BseYI C'CCAG_C
Cuts at: 6719 7215 6719
Size: 496 7020
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2749 1163
Size: 573 1013 5930
Fragments arranged by size:
5930 1013 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4966 5727 6755 1540
Size: 275 186 2965 761 1028 2301
Fragments arranged by size:
2965 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2717 3083 3226 3349 3413 3552 3595 5577 5662
Size: 366 143 123 64 139 43 1982 85
Cuts at: 5662 6733 2717
Size: 1071 3500
Fragments arranged by size:
3500 1982 1071 366 143 139 123 85
64 43
BsmI GAATG_Cn'
Cuts at: 2444 5027 5120 2444
Size: 2583 93 4840
Fragments arranged by size:
4840 2583 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2675 4333 2675
Size: 1658 5858
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2467 2637 2717 2755 2903 3083
Size: 399 615 62 170 80 38 148 180
Cuts at: 3083 3226 3349 3413 3552 3595 4199 4284 4318
Size: 143 123 64 139 43 604 85 34
Cuts at: 4318 4431 4835 5577 5662 6733 1391
Size: 113 404 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 604 404 399 180
170 148 143 139 123 113 85 85
80 64 62 43 38 34
BspEI T'CCGG_A
Cuts at: 7179 7179
Size: 7516
BspHI T'CATG_A
Cuts at: 5405 7135 5405
Size: 1730 5786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 3209 3332 3455 3701 4871 844
Size: 1937 428 123 123 246 1170 3489
Fragments arranged by size:
3489 1937 1170 428 246 123 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 4079 5403 1394
Size: 429 257 1999 1324 3507
Fragments arranged by size:
3507 1999 1324 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 6011 6185 65
Size: 1183 4763 174 1396
Fragments arranged by size:
4763 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6160 6347 1700
Size: 110 4350 187 2869
Fragments arranged by size:
4350 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7516
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7516
BssSI C'ACGA_G
Cuts at: 3125 5576 6588 3125
Size: 2451 1012 4053
Fragments arranged by size:
4053 2451 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3324 3948 794
Size: 2530 624 4362
Fragments arranged by size:
4362 2530 624
BstBI TT'CG_AA
Cuts at: 6320 6320
Size: 7516
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7516
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 2590
Size: 7516
BstYI r'GATC_y
Cuts at: 1972 2845 3515 3989 4846 5213 5598 5615 7067
Size: 873 670 474 857 367 385 17 1452
Cuts at: 7067 7153 7165 7511 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 873 857 670 474 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2474 2523 3537 2474
Size: 49 1014 6453
Fragments arranged by size:
6453 1014 49
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 2489 2532 2856 2989 3292
Size: 552 939 393 92 43 324 133 303
Cuts at: 3292 3358 3481 3604 3727 3850 4167 513
Size: 66 123 123 123 123 317 3862
Fragments arranged by size:
3862 939 552 393 324 317 303 133
123 123 123 123 92 66 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2425 3573 487
Size: 683 56 1199 1148 4430
Fragments arranged by size:
4430 1199 1148 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 3160 3637 5032 5826 5846 2209
Size: 951 477 1395 794 20 3879
Fragments arranged by size:
3879 1395 951 794 477 20
ClaI AT'CG_AT
Cuts at: 1554 3984 1554
Size: 2430 5086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7481
DraI TTT'AAA
Cuts at: 4820 4820
Size: 7516
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6523 809
Size: 507 5207 1802
Fragments arranged by size:
5207 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2535 2661
Size: 54 1425 50 275 18 588 117 126
Cuts at: 2661 4963 5852 7481 8
Size: 2302 889 1629 43
Fragments arranged by size:
2302 1629 1425 889 588 275 126 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4963 1537
Size: 275 3151 4090
Fragments arranged by size:
4090 3151 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2945 4002 4451 5446 1353
Size: 1592 1057 449 995 3423
Fragments arranged by size:
3423 1592 1057 995 449
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6103 6477 6623 387
Size: 1064 138 4514 374 146 1280
Fragments arranged by size:
4514 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 3975 4079 4530 4644 5579 6176
Size: 393 533 1810 104 451 114 935 597
Cuts at: 6176 6963 7231 1239
Size: 787 268 1524
Fragments arranged by size:
1810 1524 935 787 597 533 451 393
268 114 104
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2939 3205 3697 2347
Size: 592 266 492 6166
Fragments arranged by size:
6166 592 492 266
EcoO109I rG'GnC_Cy
Cuts at: 2463 3436 4263 2463
Size: 973 827 5716
Fragments arranged by size:
5716 973 827
EcoRI G'AATT_C
Cuts at: 3883 4825 5203 3883
Size: 942 378 6196
Fragments arranged by size:
6196 942 378
EcoRV GAT'ATC
Cuts at: 4598 4598
Size: 7516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4462 4634 4462
Size: 172 7344
FspI TGC'GCA
Cuts at: 6022 6022
Size: 7516
HaeII r_GCGC'y
Cuts at: 1678 4762 6663 1678
Size: 3084 1901 2531
Fragments arranged by size:
3084 2531 1901
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 2374 2406 3534
Size: 32 135 32 465 32 305 32 1128
Cuts at: 3534 3566 3780 3812 4313 4345 6157 6189 6231
Size: 32 214 32 501 32 1812 32 42
Cuts at: 6231 6263 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 1128 501 465 305 214 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 3136 3259 3505 4860 5106
Size: 988 281 271 927 123 246 1355 246
Cuts at: 5106 669
Size: 3079
Fragments arranged by size:
3079 1355 988 927 281 271 246 246
123
HindIII A'AGCT_T
Cuts at: 748 4637 748
Size: 3889 3627
HpaI GTT'AAC
Cuts at: 3136 5106 3136
Size: 1970 5546
KpnI G_GTAC'C
Cuts at: 4841 4841
Size: 7516
MfeI C'AATT_G
Cuts at: 3963 5115 3963
Size: 1152 6364
MluI A'CGCG_T
Cuts at: 7392 7392
Size: 7516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4597 6630 6814 1305
Size: 3292 2033 184 2007
Fragments arranged by size:
3292 2033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 2663 10
Size: 54 1425 343 588 243 4863
Fragments arranged by size:
4863 1425 588 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2220 2288 2588 2748 2871 2994 3006
Size: 648 1054 68 300 160 123 123 12
Cuts at: 3006 3129 3363 3486 3609 3621 3732 3744 3746
Size: 123 234 123 123 12 111 12 2
Cuts at: 3746 3769 3867 4277 5474 5833 5992 518
Size: 23 98 410 1197 359 159 2042
Fragments arranged by size:
2042 1197 1054 648 410 359 300 234
160 159 123 123 123 123 123 111
98 68 23 12 12 12 2
MspA1I CmG'CkG
Cuts at: 1606 3285 3373 4932 5609 6757 7002 1606
Size: 1679 88 1559 677 1148 245 2120
Fragments arranged by size:
2120 1679 1559 1148 677 245 88
NcoI C'CATG_G
Cuts at: 513 1065 2004 2856 3358 3481 3727 3850 513
Size: 552 939 852 502 123 246 123 4179
Fragments arranged by size:
4179 939 852 552 502 246 123 123
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7516
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NspI r_CATG'y
Cuts at: 1132 1229 2728 4228 4874 6419 1132
Size: 97 1499 1500 646 1545 2229
Fragments arranged by size:
2229 1545 1500 1499 646 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7516
PciI A'CATG_T
Cuts at: 2724 4224 6415 2724
Size: 1500 2191 3825
Fragments arranged by size:
3825 2191 1500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7516
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 7516
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PmlI CAC'GTG
Cuts at: 2087 3547 3771 2087
Size: 1460 224 5832
Fragments arranged by size:
5832 1460 224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 7098 7130 5585
Size: 32 1481 32 5971
Fragments arranged by size:
5971 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3436 3436
Size: 7516
PshAI GACnn'nnGTC
Cuts at: 1613 2271 1613
Size: 658 6858
PsiI TTA'TAA
Cuts at: 4712 5086 4712
Size: 374 7142
PspOMI G'GGCC_C
Cuts at: 2463 2463
Size: 7516
PspXI vC'TCGA_Gb
Cuts at: 4080 4080
Size: 7516
PstI C_TGCA'G
Cuts at: 830 2792 3206 3329 3698 4868 830
Size: 1962 414 123 369 1170 3478
Fragments arranged by size:
3478 1962 1170 414 369 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 3373 3373
Size: 7516
SalI G'TCGA_C
Cuts at: 1936 4858 1936
Size: 2922 4594
SbfI CC_TGCA'GG
Cuts at: 4868 4868
Size: 7516
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SexAI A'CCwGG_T
Cuts at: 2175 3969 2175
Size: 1794 5722
SfcI C'TryA_G
Cuts at: 826 1045 2788 2920 3202 3325 3694 3720 4864
Size: 219 1743 132 282 123 369 26 1144
Cuts at: 4864 5999 6680 6871 826
Size: 1135 681 191 1471
Fragments arranged by size:
1743 1471 1144 1135 681 369 282 219
191 132 123 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7516
SmaI CCC'GGG
Cuts at: 1872 3455 4843 1872
Size: 1583 1388 4545
Fragments arranged by size:
4545 1583 1388
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 3440 4080 4493 5620 6521
Size: 197 926 393 1104 640 413 1127 901
Cuts at: 6521 6783 820
Size: 262 1553
Fragments arranged by size:
1553 1127 1104 926 901 640 413 393
262 197
SnaBI TAC'GTA
Cuts at: 493 5307 493
Size: 4814 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7516
SphI G_CATG'C
Cuts at: 1132 4874 1132
Size: 3742 3774
SspI AAT'ATT
Cuts at: 5 52 5440 5
Size: 47 5388 2081
Fragments arranged by size:
5388 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7516
StyI C'CwwG_G
Cuts at: 513 1065 2004 2856 3358 3481 3727 3850 4246
Size: 552 939 852 502 123 246 123 396
Cuts at: 4246 4350 4955 513
Size: 104 605 3074
Fragments arranged by size:
3074 939 852 605 552 502 396 246
123 123 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 5543 5560 5713 5898 6343 325
Size: 1255 648 3315 17 153 185 445 1498
Fragments arranged by size:
3315 1498 1255 648 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5762 7474 96
Size: 274 80 33 51 1443 3785 1712 138
Fragments arranged by size:
3785 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 3556 3588 7476 7508 2941
Size: 32 583 32 3888 32 2949
Fragments arranged by size:
3888 2949 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2648 3971 1316
Size: 1332 1323 4861
Fragments arranged by size:
4861 1332 1323
XbaI T'CTAG_A
Cuts at: 4516 4852 4516
Size: 336 7180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 2921 2413
Size: 508 7008
XhoI C'TCGA_G
Cuts at: 1943 4080 1943
Size: 2137 5379
XmaI C'CCGG_G
Cuts at: 1870 3453 4841 1870
Size: 1583 1388 4545
Fragments arranged by size:
4545 1583 1388
XmnI GAAnn'nnTTC
Cuts at: 2327 5645 2327
Size: 3318 4198
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4327 5324 276
Size: 53 83 186 936 2793 997 2468
Fragments arranged by size:
2793 2468 997 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI