Restriction map for
pFN21AB8522
(Circular) (Six-base) MAPSORT of: pFN21AB8522.seq Check: 2943 from: 1 to: 7342
pFN21AB8522 7342 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5152 278
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4154 4685 1937
Size: 2217 531 4594
Fragments arranged by size:
4594 2217 531
Acc65I G'GTAC_C
Cuts at: 4663 4663
Size: 7342
AclI AA'CG_TT
Cuts at: 1563 5469 5842 1563
Size: 3906 373 3063
Fragments arranged by size:
3906 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 5405 6789 7057 1632
Size: 555 3218 1384 268 1917
Fragments arranged by size:
3218 1917 1384 555 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7145
AflIII A'CryG_T
Cuts at: 1203 2297 2640 3405 6241 7218 1203
Size: 1094 343 765 2836 977 1327
Fragments arranged by size:
2836 1327 1094 977 765 343
AgeI A'CCGG_T
Cuts at: 6173 6173
Size: 7342
AhdI GACnn_n'nnGTC
Cuts at: 6071 6071
Size: 7342
AleI CACnn'nnGTG
Cuts at: 1166 2894 4586 1166
Size: 1728 1692 3922
Fragments arranged by size:
3922 1728 1692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4604 1184
Size: 3420 3922
AlwNI CAG_nnn'CTG
Cuts at: 5085 6657 5085
Size: 1572 5770
ApaLI G'TGCA_C
Cuts at: 5399 6555 5399
Size: 1156 6186
ApoI r'AATT_y
Cuts at: 1493 4046 4294 4651 4877 5029 7138 1493
Size: 2553 248 357 226 152 2109 1697
Fragments arranged by size:
2553 2109 1697 357 248 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5896 160
Size: 872 4864 1606
Fragments arranged by size:
4864 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
AvaI C'yCGr_G
Cuts at: 1870 1943 3941 4667 1870
Size: 73 1998 726 4545
Fragments arranged by size:
4545 1998 726 73
BamHI G'GATC_C
Cuts at: 2393 4672 5039 2393
Size: 2279 367 4696
Fragments arranged by size:
4696 2279 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2960 4142 4663 6118 618
Size: 325 217 627 1173 1182 521 1455 1842
Fragments arranged by size:
1842 1455 1182 1173 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2856 4661 1391
Size: 1465 1805 4072
Fragments arranged by size:
4072 1805 1465
BbeI G_GCGC'C
Cuts at: 2964 2964
Size: 7342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 3729 928
Size: 971 297 1533 4541
Fragments arranged by size:
4541 1533 971 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3524 3558 3828 3862 5533 5567 1903
Size: 34 1587 34 270 34 1671 34 3678
Fragments arranged by size:
3678 1671 1587 270 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 2302 5234 6444 2287
Size: 15 2932 1210 3185
Fragments arranged by size:
3185 2932 1210 15
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7342
BfrBI ATG'CAT
Cuts at: 2318 3780 2318
Size: 1462 5880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4434 5953 136
Size: 107 122 71 631 605 2762 1519 1525
Fragments arranged by size:
2762 1525 1519 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2613 7337 2613
Size: 4724 2618
BlpI GC'TnA_GC
Cuts at: 4759 4759
Size: 7342
Bme1580I G_kGCm'C
Cuts at: 1790 2580 3547 4289 5403 6559 1790
Size: 790 967 742 1114 1156 2573
Fragments arranged by size:
2573 1156 1114 967 790 742
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3469 3520 3737
Size: 416 53 83 399 24 2052 51 217
Cuts at: 3737 4053 4560 5073 6031 442
Size: 316 507 513 958 1753
Fragments arranged by size:
2052 1753 958 513 507 416 399 316
217 83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 7342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2993 3077 4151 6002 1239
Size: 1115 639 84 1074 1851 2579
Fragments arranged by size:
2579 1851 1115 1074 639 84
Bpu10I CC'TnA_GC
Cuts at: 2038 2038
Size: 7342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5467 6332 6630 5467
Size: 865 298 6179
Fragments arranged by size:
6179 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6005 882
Size: 617 4506 2219
Fragments arranged by size:
4506 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 4338 4584 5133 493
Size: 711 1437 1697 246 549 2702
Fragments arranged by size:
2702 1697 1437 711 549 246
BsaBI GATnn'nnATC
Cuts at: 3596 7336 3596
Size: 3740 3602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2961 3726 5149 5531
Size: 53 83 186 936 1428 765 1423 382
Cuts at: 5531 6166 275
Size: 635 1451
Fragments arranged by size:
1451 1428 1423 936 765 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5775 6173 6447 6594 7005 5775
Size: 398 274 147 411 6112
Fragments arranged by size:
6112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 1178
Size: 30 165 30 1659 30 5428
Fragments arranged by size:
5428 1659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7342
BseYI C'CCAG_C
Cuts at: 2910 2985 3946 6545 7041 2910
Size: 75 961 2599 496 3211
Fragments arranged by size:
3211 2599 961 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3106 3550 4418 4505 1163
Size: 573 1370 444 868 87 4000
Fragments arranged by size:
4000 1370 868 573 444 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 4792 5553 6581 1540
Size: 275 186 1006 1785 761 1028 2301
Fragments arranged by size:
2301 1785 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2856 5403 5488 6559 2856
Size: 2547 85 1071 3639
Fragments arranged by size:
3639 2547 1071 85
BsmI GAATG_Cn'
Cuts at: 4853 4946 4853
Size: 93 7249
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2580 2856 3547 4289 4661 5403 5488
Size: 399 790 276 691 742 372 742 85
Cuts at: 5488 6559 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 790 742 742 691 399 372
276 85
BspEI T'CCGG_A
Cuts at: 7005 7005
Size: 7342
BspHI T'CATG_A
Cuts at: 5231 6961 5231
Size: 1730 5612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2490 4697 844
Size: 1646 2207 3489
Fragments arranged by size:
3489 2207 1646
BsrBI CCG'CTC
Cuts at: 1394 1823 2751 5229 1394
Size: 429 928 2478 3507
Fragments arranged by size:
3507 2478 928 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 2856 3750 3900 3929 3999 4201
Size: 1183 1179 429 894 150 29 70 202
Cuts at: 4201 4609 5837 6011 65
Size: 408 1228 174 1396
Fragments arranged by size:
1396 1228 1183 1179 894 429 408 202
174 150 70 29
BsrFI r'CCGG_y
Cuts at: 1700 1810 5986 6173 1700
Size: 110 4176 187 2869
Fragments arranged by size:
4176 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3294 3773 4329 96
Size: 3198 479 556 3109
Fragments arranged by size:
3198 3109 556 479
BssHII G'CGCG_C
Cuts at: 1925 2810 2916 1925
Size: 885 106 6351
Fragments arranged by size:
6351 885 106
BssSI C'ACGA_G
Cuts at: 5402 6414 5402
Size: 1012 6330
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2721 3223 794
Size: 1927 502 4913
Fragments arranged by size:
4913 1927 502
BstBI TT'CG_AA
Cuts at: 6146 6146
Size: 7342
BstEII G'GTnAC_C
Cuts at: 1184 2114 1184
Size: 930 6412
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2605 2971 2473
Size: 132 366 6844
Fragments arranged by size:
6844 366 132
BstYI r'GATC_y
Cuts at: 1972 2393 2499 2613 3273 4672 5039 5424 5441
Size: 421 106 114 660 1399 367 385 17
Cuts at: 5441 6893 6979 6991 7337 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1399 660 421 385 367 346
114 106 86 17 12
BstZ17I GTA'TAC
Cuts at: 4155 4155
Size: 7342
Bsu36I CC'TnA_GG
Cuts at: 3702 3702
Size: 7342
BtgI C'CryG_G
Cuts at: 513 1065 2604 3201 513
Size: 552 1539 597 4654
Fragments arranged by size:
4654 1539 597 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2710 487
Size: 683 56 1484 5119
Fragments arranged by size:
5119 1484 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 3669 4858 5652 5672 2464
Size: 1205 1189 794 20 4134
Fragments arranged by size:
4134 1205 1189 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7307
DraI TTT'AAA
Cuts at: 4646 4646
Size: 7342
DraIII CAC_nnn'GTG
Cuts at: 4338 4338
Size: 7342
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2641 6349 809
Size: 507 1325 3708 1802
Fragments arranged by size:
3708 1802 1325 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 3135 4789
Size: 54 1425 50 275 18 1068 237 1654
Cuts at: 4789 5678 7307 8
Size: 889 1629 43
Fragments arranged by size:
1654 1629 1425 1068 889 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4789 1537
Size: 275 2977 4090
Fragments arranged by size:
4090 2977 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 2831 5272 1353
Size: 1145 333 2441 3423
Fragments arranged by size:
3423 2441 1145 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2750 2984 5929 6303 6449 387
Size: 1064 138 1161 234 2945 374 146 1280
Fragments arranged by size:
2945 1280 1161 1064 374 234 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 7342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2354 2993 3077 4151 5405 6002
Size: 393 555 167 639 84 1074 1254 597
Cuts at: 6002 6789 7057 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1254 1074 787 639 597 555 393
268 167 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3701 3701
Size: 7342
EcoO109I rG'GnC_Cy
Cuts at: 2131 4135 2131
Size: 2004 5338
EcoRI G'AATT_C
Cuts at: 4651 5029 4651
Size: 378 6964
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2135 3322 2135
Size: 1187 6155
FspI TGC'GCA
Cuts at: 2714 2834 5848 2714
Size: 120 3014 4208
Fragments arranged by size:
4208 3014 120
HaeII r_GCGC'y
Cuts at: 1678 2532 2964 4035 6489 1678
Size: 854 432 1071 2454 2531
Fragments arranged by size:
2531 2454 1071 854 432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 3149 3181 3473
Size: 32 135 32 477 32 1068 32 292
Cuts at: 3473 3505 3638 3670 4509 4541 5983 6015 6057
Size: 32 133 32 839 32 1442 32 42
Cuts at: 6057 6089 1373
Size: 32 2626
Fragments arranged by size:
2626 1442 1068 839 477 292 135 133
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2016 3487 4686 4932 669
Size: 988 281 78 1471 1199 246 3079
Fragments arranged by size:
3079 1471 1199 988 281 246 78
HindIII A'AGCT_T
Cuts at: 748 3254 748
Size: 2506 4836
HpaI GTT'AAC
Cuts at: 4932 4932
Size: 7342
KasI G'GCGC_C
Cuts at: 2960 2960
Size: 7342
KpnI G_GTAC'C
Cuts at: 4667 4667
Size: 7342
MfeI C'AATT_G
Cuts at: 4941 4941
Size: 7342
MluI A'CGCG_T
Cuts at: 7218 7218
Size: 7342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 6456 6640 1305
Size: 1473 3678 184 2007
Fragments arranged by size:
3678 2007 1473 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2900 3137 10
Size: 54 1425 343 1068 237 4215
Fragments arranged by size:
4215 1425 1068 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2471 2894 3179 4586 5300 5659
Size: 648 843 462 423 285 1407 714 359
Cuts at: 5659 5818 518
Size: 159 2042
Fragments arranged by size:
2042 1407 843 714 648 462 423 359
285 159
MspA1I CmG'CkG
Cuts at: 1606 4758 5435 6583 6828 1606
Size: 3152 677 1148 245 2120
Fragments arranged by size:
3152 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2961 2961
Size: 7342
NcoI C'CATG_G
Cuts at: 513 1065 2604 3201 513
Size: 552 1539 597 4654
Fragments arranged by size:
4654 1539 597 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7342
NotI GC'GGCC_GC
Cuts at: 4789 4789
Size: 7342
NsiI A_TGCA'T
Cuts at: 2320 3782 2320
Size: 1462 5880
NspI r_CATG'y
Cuts at: 1132 1229 2301 2951 3780 4216 4700 6245 1132
Size: 97 1072 650 829 436 484 1545 2229
Fragments arranged by size:
2229 1545 1072 829 650 484 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7342
PciI A'CATG_T
Cuts at: 2297 6241 2297
Size: 3944 3398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7342
PfoI T'CCnGG_A
Cuts at: 3057 3276 3503 4128 3057
Size: 219 227 625 6271
Fragments arranged by size:
6271 625 227 219
PmeI GTTT'AAAC
Cuts at: 4646 4646
Size: 7342
PmlI CAC'GTG
Cuts at: 4338 4338
Size: 7342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5411 5443 6924 6956 5411
Size: 32 1481 32 5797
Fragments arranged by size:
5797 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7342
PsiI TTA'TAA
Cuts at: 4912 4912
Size: 7342
PstI C_TGCA'G
Cuts at: 830 3676 4694 830
Size: 2846 1018 3478
Fragments arranged by size:
3478 2846 1018
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7342
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 7342
SalI G'TCGA_C
Cuts at: 1936 4684 1936
Size: 2748 4594
SapI GCTCTTCn'nnn_
Cuts at: 2498 2498
Size: 7342
SbfI CC_TGCA'GG
Cuts at: 4694 4694
Size: 7342
ScaI AGT'ACT
Cuts at: 5590 7302 5590
Size: 1712 5630
SfcI C'TryA_G
Cuts at: 826 1045 3672 4390 4690 5825 6506 6697 826
Size: 219 2627 718 300 1135 681 191 1471
Fragments arranged by size:
2627 1471 1135 718 681 300 219 191
SfoI GGC'GCC
Cuts at: 2962 2962
Size: 7342
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7342
SmaI CCC'GGG
Cuts at: 1872 4669 1872
Size: 2797 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5446 6347 6609 820
Size: 197 926 3503 901 262 1553
Fragments arranged by size:
3503 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5133 493
Size: 4640 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7342
SphI G_CATG'C
Cuts at: 1132 4700 1132
Size: 3568 3774
SspI AAT'ATT
Cuts at: 5 52 5266 5
Size: 47 5214 2081
Fragments arranged by size:
5214 2081 47
StuI AGG'CCT
Cuts at: 1517 2690 1517
Size: 1173 6169
StyI C'CwwG_G
Cuts at: 513 1065 2604 3201 3819 4467 4781 513
Size: 552 1539 597 618 648 314 3074
Fragments arranged by size:
3074 1539 648 618 597 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2208 2267 2884 4480 5369 5386 5539
Size: 1255 628 59 617 1596 889 17 153
Cuts at: 5539 5724 6169 325
Size: 185 445 1498
Fragments arranged by size:
1596 1498 1255 889 628 617 445 185
153 59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3294 3773 4329
Size: 274 80 33 51 1443 1317 479 556
Cuts at: 4329 5588 7300 96
Size: 1259 1712 138
Fragments arranged by size:
1712 1443 1317 1259 556 479 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3223 3255 7302 7334 3223
Size: 32 4047 32 3231
Fragments arranged by size:
4047 3231 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7342
XbaI T'CTAG_A
Cuts at: 4678 4678
Size: 7342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3425 3757 4636 3425
Size: 332 879 6131
Fragments arranged by size:
6131 879 332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7342
XmaI C'CCGG_G
Cuts at: 1870 4667 1870
Size: 2797 4545
XmnI GAAnn'nnTTC
Cuts at: 3436 5471 3436
Size: 2035 5307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5150 276
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BsmBI EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SrfI SwaI