Restriction map for
pFN21AB8519
(Circular) (Six-base) MAPSORT of: pFN21AB8519.seq Check: 573 from: 1 to: 7564
pFN21AB8519 7564 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4211 4211
Size: 7564
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2490 5374 278
Size: 53 83 186 936 954 2884 2468
Fragments arranged by size:
2884 2468 954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2130 3755 4907 1937
Size: 193 1625 1152 4594
Fragments arranged by size:
4594 1625 1152 193
Acc65I G'GTAC_C
Cuts at: 4885 4885
Size: 7564
AclI AA'CG_TT
Cuts at: 1563 5691 6064 1563
Size: 4128 373 3063
Fragments arranged by size:
4128 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 4059 4766 5627 7011 7279 1632
Size: 750 1677 707 861 1384 268 1917
Fragments arranged by size:
1917 1677 1384 861 750 707 268
AflII C'TTAA_G
Cuts at: 820 1017 2060 2546 3398 4670 820
Size: 197 1043 486 852 1272 3714
Fragments arranged by size:
3714 1272 1043 852 486 197
AflIII A'CryG_T
Cuts at: 1203 3814 6463 7440 1203
Size: 2611 2649 977 1327
Fragments arranged by size:
2649 2611 1327 977
AgeI A'CCGG_T
Cuts at: 6395 6395
Size: 7564
AhdI GACnn_n'nnGTC
Cuts at: 2486 6293 2486
Size: 3807 3757
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7564
AlwNI CAG_nnn'CTG
Cuts at: 5307 6879 5307
Size: 1572 5992
ApaLI G'TGCA_C
Cuts at: 5621 6777 5621
Size: 1156 6408
ApoI r'AATT_y
Cuts at: 1493 2066 2115 2985 3088 3206 4873 5099 5251
Size: 573 49 870 103 118 1667 226 152
Cuts at: 5251 7360 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1667 870 573 226 152 118
103 49
AseI AT'TA_AT
Cuts at: 160 1032 4011 6118 160
Size: 872 2979 2107 1606
Fragments arranged by size:
2979 2107 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
AvaI C'yCGr_G
Cuts at: 1870 1943 4650 4889 1870
Size: 73 2707 239 4545
Fragments arranged by size:
4545 2707 239 73
AvrII C'CTAG_G
Cuts at: 3550 3550
Size: 7564
BamHI G'GATC_C
Cuts at: 2843 4894 5261 2843
Size: 2051 367 5146
Fragments arranged by size:
5146 2051 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 4885 6340 618
Size: 325 217 627 1032 2066 1455 1842
Fragments arranged by size:
2066 1842 1455 1032 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3572 4883 1391
Size: 2181 1311 4072
Fragments arranged by size:
4072 2181 1311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2485 2727 4767 928
Size: 971 586 242 2040 3725
Fragments arranged by size:
3725 2040 971 586 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3400 3434 5755 5789 1903
Size: 34 1463 34 2321 34 3678
Fragments arranged by size:
3678 2321 1463 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3286 4744 5456 6666 3286
Size: 1458 712 1210 4184
Fragments arranged by size:
4184 1458 1210 712
BclI T'GATC_A
Cuts at: 1548 2007 2320 2434 1548
Size: 459 313 114 6678
Fragments arranged by size:
6678 459 313 114
BfrBI ATG'CAT
Cuts at: 2316 2316
Size: 7564
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2192 6175 136
Size: 107 122 71 631 605 520 3983 1525
Fragments arranged by size:
3983 1525 631 605 520 122 107 71
BglII A'GATC_T
Cuts at: 7559 7559
Size: 7564
BlpI GC'TnA_GC
Cuts at: 4981 4981
Size: 7564
Bme1580I G_kGCm'C
Cuts at: 1790 3905 4066 5625 6781 1790
Size: 2115 161 1559 1156 2573
Fragments arranged by size:
2573 2115 1559 1156 161
BmgBI CAC'GTC
Cuts at: 1318 3473 1318
Size: 2155 5409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3208 4269 4300
Size: 416 53 83 399 24 1791 1061 31
Cuts at: 4300 5295 6253 442
Size: 995 958 1753
Fragments arranged by size:
1791 1753 1061 995 958 416 399 83
53 31 24
BmtI G_CTAG'C
Cuts at: 1056 2775 1056
Size: 1719 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 2528 3285 6224 1239
Size: 1250 39 757 2939 2579
Fragments arranged by size:
2939 2579 1250 757 39
Bpu10I CC'TnA_GC
Cuts at: 3582 4617 3582
Size: 1035 6529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5689 6554 6852 5689
Size: 865 298 6401
Fragments arranged by size:
6401 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3335 4685 6227 882
Size: 617 1836 1350 1542 2219
Fragments arranged by size:
2219 1836 1542 1350 617
BsaAI yAC'GTr
Cuts at: 493 1204 4051 5355 493
Size: 711 2847 1304 2702
Fragments arranged by size:
2847 2702 1304 711
BsaBI GATnn'nnATC
Cuts at: 7558 7558
Size: 7564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2487 3805 4290 5371
Size: 53 83 186 936 954 1318 485 1081
Cuts at: 5371 5753 6388 275
Size: 382 635 1451
Fragments arranged by size:
1451 1318 1081 954 936 635 485 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2166 5997 6395 6669 6816 7227 2166
Size: 3831 398 274 147 411 2503
Fragments arranged by size:
3831 2503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7339
Fragments arranged by size:
7339 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 4568 4571 4784 4826 4829 1186
Size: 1057 2325 3 213 42 3 3921
Fragments arranged by size:
3921 2325 1057 213 42 3 3
BseYI C'CCAG_C
Cuts at: 4645 4665 6767 7263 4645
Size: 20 2102 496 4946
Fragments arranged by size:
4946 2102 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3453 1163
Size: 573 1717 5274
Fragments arranged by size:
5274 1717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 5014 5775 6803 1540
Size: 275 186 18 2995 761 1028 2301
Fragments arranged by size:
2995 2301 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 5625 5710 6781 5625
Size: 85 1071 6408
Fragments arranged by size:
6408 1071 85
BsmI GAATG_Cn'
Cuts at: 2092 5075 5168 2092
Size: 2983 93 4488
Fragments arranged by size:
4488 2983 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3572 3905 4066 4883 5625 5710 6781
Size: 399 1782 333 161 817 742 85 1071
Cuts at: 6781 1391
Size: 2174
Fragments arranged by size:
2174 1782 1071 817 742 399 333 161
85
BspEI T'CCGG_A
Cuts at: 2166 7227 2166
Size: 5061 2503
BspHI T'CATG_A
Cuts at: 3054 3247 4086 5453 7183 3054
Size: 193 839 1367 1730 3435
Fragments arranged by size:
3435 1730 1367 839 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4211 4919 844
Size: 3367 708 3489
Fragments arranged by size:
3489 3367 708
BsrBI CCG'CTC
Cuts at: 1394 1823 5451 1394
Size: 429 3628 3507
Fragments arranged by size:
3628 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 3418 3969 6059 6233 65
Size: 1183 1212 958 551 2090 174 1396
Fragments arranged by size:
2090 1396 1212 1183 958 551 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6208 6395 1700
Size: 110 4398 187 2869
Fragments arranged by size:
4398 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7564
BssSI C'ACGA_G
Cuts at: 5624 6636 5624
Size: 1012 6552
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4445 794
Size: 3651 3913
BstBI TT'CG_AA
Cuts at: 2809 3739 6368 2809
Size: 930 2629 4005
Fragments arranged by size:
4005 2629 930
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7564
BstYI r'GATC_y
Cuts at: 1972 2843 4894 5261 5646 5663 7115 7201 7213
Size: 871 2051 367 385 17 1452 86 12
Cuts at: 7213 7559 1972
Size: 346 1977
Fragments arranged by size:
2051 1977 1452 871 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3756 3756
Size: 7564
Bsu36I CC'TnA_GG
Cuts at: 2682 2958 2682
Size: 276 7288
BtgI C'CryG_G
Cuts at: 513 1065 3459 3765 513
Size: 552 2394 306 4312
Fragments arranged by size:
4312 2394 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2437 487
Size: 683 56 1211 5614
Fragments arranged by size:
5614 1211 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 5080 5874 5894 2923
Size: 2157 794 20 4593
Fragments arranged by size:
4593 2157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3346 3381 529
Size: 35 2782 35 4712
Fragments arranged by size:
4712 2782 35 35
DraI TTT'AAA
Cuts at: 4868 4868
Size: 7564
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6571 809
Size: 507 5255 1802
Fragments arranged by size:
5255 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 4179 5011
Size: 54 1425 50 275 18 186 2163 832
Cuts at: 5011 5900 7529 8
Size: 889 1629 43
Fragments arranged by size:
2163 1629 1425 889 832 275 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 5011 1537
Size: 275 204 2995 4090
Fragments arranged by size:
4090 2995 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2270 3681 5494 1353
Size: 687 230 1411 1813 3423
Fragments arranged by size:
3423 1813 1411 687 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6151 6525 6671 387
Size: 1064 138 4562 374 146 1280
Fragments arranged by size:
4562 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2489 2528 3285 4059 4766 5627
Size: 393 750 107 39 757 774 707 861
Cuts at: 5627 6224 7011 7279 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 861 787 774 757 750 707 597
393 268 107 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3287 3509 4379 3287
Size: 222 870 6472
Fragments arranged by size:
6472 870 222
EcoO109I rG'GnC_Cy
Cuts at: 2260 3727 4675 2260
Size: 1467 948 5149
Fragments arranged by size:
5149 1467 948
EcoRI G'AATT_C
Cuts at: 3088 4873 5251 3088
Size: 1785 378 5401
Fragments arranged by size:
5401 1785 378
EcoRV GAT'ATC
Cuts at: 2698 3418 2698
Size: 720 6844
FspI TGC'GCA
Cuts at: 6070 6070
Size: 7564
HaeII r_GCGC'y
Cuts at: 1678 4194 6711 1678
Size: 2516 2517 2531
Fragments arranged by size:
2531 2517 2516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 2725 2757 2873
Size: 32 135 32 900 32 221 32 116
Cuts at: 2873 2905 4722 4754 6205 6237 6279 6311 1373
Size: 32 1817 32 1451 32 42 32 2626
Fragments arranged by size:
2626 1817 1451 900 221 135 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4908 5154 669
Size: 988 281 2970 246 3079
Fragments arranged by size:
3079 2970 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7564
HpaI GTT'AAC
Cuts at: 5154 5154
Size: 7564
KpnI G_GTAC'C
Cuts at: 4889 4889
Size: 7564
MfeI C'AATT_G
Cuts at: 2379 5163 2379
Size: 2784 4780
MluI A'CGCG_T
Cuts at: 7440 7440
Size: 7564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6678 6862 1305
Size: 5373 184 2007
Fragments arranged by size:
5373 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4181 10
Size: 54 1425 343 2349 3393
Fragments arranged by size:
3393 2349 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3366 4170 5522 5881 6040 518
Size: 648 2200 804 1352 359 159 2042
Fragments arranged by size:
2200 2042 1352 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 3539 4138 4980 5657 6805 7050 1606
Size: 415 1518 599 842 677 1148 245 2120
Fragments arranged by size:
2120 1518 1148 842 677 599 415 245
NcoI C'CATG_G
Cuts at: 513 1065 3459 3765 513
Size: 552 2394 306 4312
Fragments arranged by size:
4312 2394 552 306
NdeI CA'TA_TG
Cuts at: 387 4272 387
Size: 3885 3679
NheI G'CTAG_C
Cuts at: 1052 2771 1052
Size: 1719 5845
NotI GC'GGCC_GC
Cuts at: 5011 5011
Size: 7564
NsiI A_TGCA'T
Cuts at: 2318 2318
Size: 7564
NspI r_CATG'y
Cuts at: 1132 1229 2560 3818 4922 6467 1132
Size: 97 1331 1258 1104 1545 2229
Fragments arranged by size:
2229 1545 1331 1258 1104 97
PasI CC'CwG_GG
Cuts at: 1348 4204 1348
Size: 2856 4708
PciI A'CATG_T
Cuts at: 3814 6463 3814
Size: 2649 4915
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 3198 4636 1108
Size: 1284 806 1438 4036
Fragments arranged by size:
4036 1438 1284 806
PfoI T'CCnGG_A
Cuts at: 2866 3301 4253 2866
Size: 435 952 6177
Fragments arranged by size:
6177 952 435
PmeI GTTT'AAAC
Cuts at: 4868 4868
Size: 7564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5633 5665 7146 7178 5633
Size: 32 1481 32 6019
Fragments arranged by size:
6019 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 3727 4675 2260
Size: 1467 948 5149
Fragments arranged by size:
5149 1467 948
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7564
PsiI TTA'TAA
Cuts at: 5134 5134
Size: 7564
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2586 2618 2586
Size: 32 7532
PstI C_TGCA'G
Cuts at: 830 3024 4916 830
Size: 2194 1892 3478
Fragments arranged by size:
3478 2194 1892
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7564
PvuII CAG'CTG
Cuts at: 3539 4138 3539
Size: 599 6965
SalI G'TCGA_C
Cuts at: 1936 4906 1936
Size: 2970 4594
SbfI CC_TGCA'GG
Cuts at: 4916 4916
Size: 7564
ScaI AGT'ACT
Cuts at: 2595 5812 7524 2595
Size: 3217 1712 2635
Fragments arranged by size:
3217 2635 1712
SfcI C'TryA_G
Cuts at: 826 1045 3020 3216 3759 4696 4912 6047 6728
Size: 219 1975 196 543 937 216 1135 681
Cuts at: 6728 6919 826
Size: 191 1471
Fragments arranged by size:
1975 1471 1135 937 681 543 219 216
196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7564
SmaI CCC'GGG
Cuts at: 1872 4891 1872
Size: 3019 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 2546 3398 4670 5668 6569
Size: 197 926 117 486 852 1272 998 901
Cuts at: 6569 6831 820
Size: 262 1553
Fragments arranged by size:
1553 1272 998 926 901 852 486 262
197 117
SnaBI TAC'GTA
Cuts at: 493 5355 493
Size: 4862 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7564
SphI G_CATG'C
Cuts at: 1132 4922 1132
Size: 3790 3774
SspI AAT'ATT
Cuts at: 5 52 5488 5
Size: 47 5436 2081
Fragments arranged by size:
5436 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7564
StyI C'CwwG_G
Cuts at: 513 1065 3288 3445 3459 3550 3765 3969 4515
Size: 552 2223 157 14 91 215 204 546
Cuts at: 4515 5003 513
Size: 488 3074
Fragments arranged by size:
3074 2223 552 546 488 215 204 157
91 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 5591 5608 5761 5946 6391 325
Size: 1255 1638 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1638 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2593 5810 7522
Size: 274 80 33 51 1443 616 3217 1712
Cuts at: 7522 96
Size: 138
Fragments arranged by size:
3217 1712 1443 616 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3133 3165 3967 3999 7524 7556 3133
Size: 32 802 32 3525 32 3141
Fragments arranged by size:
3525 3141 802 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7564
XbaI T'CTAG_A
Cuts at: 2428 4900 2428
Size: 2472 5092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2338
Size: 7564
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7564
XmaI C'CCGG_G
Cuts at: 1870 4889 1870
Size: 3019 4545
XmnI GAAnn'nnTTC
Cuts at: 2029 4127 5693 2029
Size: 2098 1566 3900
Fragments arranged by size:
3900 2098 1566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2488 5372 276
Size: 53 83 186 936 954 2884 2468
Fragments arranged by size:
2884 2468 954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI