Restriction map for
pFN21AB8508
(Circular) (Six-base) MAPSORT of: pFN21AB8508.seq Check: 5212 from: 1 to: 7303
pFN21AB8508 7303 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4646 1937
Size: 2709 4594
Acc65I G'GTAC_C
Cuts at: 2734 3929 4624 2734
Size: 1195 695 5413
Fragments arranged by size:
5413 1195 695
AclI AA'CG_TT
Cuts at: 1563 5430 5803 1563
Size: 3867 373 3063
Fragments arranged by size:
3867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2447 2606 2711 3909 4269 5366 6750
Size: 480 335 159 105 1198 360 1097 1384
Cuts at: 6750 7018 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1198 1097 480 360 335 268
159 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 3374 6202 7179 1203
Size: 2171 2828 977 1327
Fragments arranged by size:
2828 2171 1327 977
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3070 3102 3070
Size: 32 7271
AlwNI CAG_nnn'CTG
Cuts at: 2467 3203 3656 3897 5046 6618 2467
Size: 736 453 241 1149 1572 3152
Fragments arranged by size:
3152 1572 1149 736 453 241
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 2272 2337 2710 3710 4054 4121 4612 4838
Size: 779 65 373 1000 344 67 491 226
Cuts at: 4838 4990 7099 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1000 779 491 373 344 226
152 67 65
AseI AT'TA_AT
Cuts at: 160 1032 5857 160
Size: 872 4825 1606
Fragments arranged by size:
4825 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 2814 3479 4628 1870
Size: 73 871 665 1149 4545
Fragments arranged by size:
4545 1149 871 665 73
BamHI G'GATC_C
Cuts at: 3965 4633 5000 3965
Size: 668 367 6268
Fragments arranged by size:
6268 668 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3540 3929 4624 6079
Size: 325 217 627 947 806 389 695 1455
Cuts at: 6079 618
Size: 1842
Fragments arranged by size:
1842 1455 947 806 695 627 389 325
217
BanII G_rGCy'C
Cuts at: 1391 2488 2903 2942 2981 3020 3059 3137 3330
Size: 1097 415 39 39 39 39 78 193
Cuts at: 3330 3650 4169 4481 4622 1391
Size: 320 519 312 141 4072
Fragments arranged by size:
4072 1097 519 415 320 312 193 141
78 39 39 39 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 6047
Fragments arranged by size:
6047 971 285
BbvCI CC'TCA_GC
Cuts at: 2359 2359
Size: 7303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2535 2569 5494 5528 1903
Size: 34 598 34 2925 34 3678
Fragments arranged by size:
3678 2925 598 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 5914 136
Size: 107 122 71 631 605 530 3712 1525
Fragments arranged by size:
3712 1525 631 605 530 122 107 71
BglII A'GATC_T
Cuts at: 2581 7298 2581
Size: 4717 2586
BlpI GC'TnA_GC
Cuts at: 4720 4720
Size: 7303
Bme1580I G_kGCm'C
Cuts at: 1790 2051 3120 3239 5364 6520 1790
Size: 261 1069 119 2125 1156 2573
Fragments arranged by size:
2573 2125 1156 1069 261 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 4204 5034
Size: 416 53 83 399 24 1824 963 830
Cuts at: 5034 5992 442
Size: 958 1753
Fragments arranged by size:
1824 1753 963 958 830 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 7303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2970 5963 1239
Size: 1292 439 2993 2579
Fragments arranged by size:
2993 2579 1292 439
Bpu10I CC'TnA_GC
Cuts at: 2359 3329 2359
Size: 970 6333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5428 6293 6591 5428
Size: 865 298 6140
Fragments arranged by size:
6140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2785 3995 5966 882
Size: 617 1286 1210 1971 2219
Fragments arranged by size:
2219 1971 1286 1210 617
BsaAI yAC'GTr
Cuts at: 493 1204 5094 493
Size: 711 3890 2702
Fragments arranged by size:
3890 2702 711
BsaBI GATnn'nnATC
Cuts at: 7297 7297
Size: 7303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5110 5492 6127 275
Size: 53 83 186 936 3577 382 635 1451
Fragments arranged by size:
3577 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2840 3074 4062 5736 6134 6408 6555 6966 2840
Size: 234 988 1674 398 274 147 411 3177
Fragments arranged by size:
3177 1674 988 411 398 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 3609 3639 1178
Size: 30 165 30 685 30 1491 30 4842
Fragments arranged by size:
4842 1491 685 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 2832 3792 4261 1186
Size: 926 720 960 469 4228
Fragments arranged by size:
4228 960 926 720 469
BseYI C'CCAG_C
Cuts at: 3113 4199 6506 7002 3113
Size: 1086 2307 496 3414
Fragments arranged by size:
3414 2307 1086 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4422 1163
Size: 573 2686 4044
Fragments arranged by size:
4044 2686 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4753 5514 6542 1540
Size: 275 186 2752 761 1028 2301
Fragments arranged by size:
2752 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2488 2903 2942 2981 3020 3137 3330 5364 5449
Size: 415 39 39 39 117 193 2034 85
Cuts at: 5449 6520 2488
Size: 1071 3271
Fragments arranged by size:
3271 2034 1071 415 193 117 85 39
39 39
BsmI GAATG_Cn'
Cuts at: 4814 4907 4814
Size: 93 7210
BsmBI CGTCTCn'nnnn_
Cuts at: 2843 2843
Size: 7303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2488 2903 2942 2981 3020 3059
Size: 399 261 437 415 39 39 39 39
Cuts at: 3059 3120 3137 3239 3330 3650 4169 4481 4622
Size: 61 17 102 91 320 519 312 141
Cuts at: 4622 5364 5449 6520 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 437 415 399 320
312 261 141 102 91 85 61 39
39 39 39 17
BspEI T'CCGG_A
Cuts at: 6966 6966
Size: 7303
BspHI T'CATG_A
Cuts at: 3841 5192 6922 3841
Size: 1351 1730 4222
Fragments arranged by size:
4222 1730 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4658 844
Size: 3814 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5190 1394
Size: 429 3367 3507
Fragments arranged by size:
3507 3367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2625 3492 4141 5798 5972 65
Size: 1183 1377 867 649 1657 174 1396
Fragments arranged by size:
1657 1396 1377 1183 867 649 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5947 6134 1700
Size: 110 4137 187 2869
Fragments arranged by size:
4137 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7303
BssSI C'ACGA_G
Cuts at: 2571 3763 5363 6375 2571
Size: 1192 1600 1012 3499
Fragments arranged by size:
3499 1600 1192 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2060 794
Size: 1266 6037
BstBI TT'CG_AA
Cuts at: 6107 6107
Size: 7303
BstEII G'GTnAC_C
Cuts at: 1184 3349 3451 1184
Size: 2165 102 5036
Fragments arranged by size:
5036 2165 102
BstXI CCAn_nnnn'nTGG
Cuts at: 3050 3448 3050
Size: 398 6905
BstYI r'GATC_y
Cuts at: 1972 2401 2532 2581 3965 4633 5000 5385 5402
Size: 429 131 49 1384 668 367 385 17
Cuts at: 5402 6854 6940 6952 7298 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1384 668 429 385 367 346
131 86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 4562 4562
Size: 7303
BtgI C'CryG_G
Cuts at: 513 1065 3721 513
Size: 552 2656 4095
Fragments arranged by size:
4095 2656 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6564
Fragments arranged by size:
6564 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3567 4284 4819 5613 5633 2881
Size: 686 717 535 794 20 4551
Fragments arranged by size:
4551 794 717 686 535 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2103 2138 529
Size: 35 1539 35 5694
Fragments arranged by size:
5694 1539 35 35
DraI TTT'AAA
Cuts at: 4607 4607
Size: 7303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6310 809
Size: 507 4994 1802
Fragments arranged by size:
4994 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4750 5639 7268
Size: 54 1425 50 275 18 2920 889 1629
Cuts at: 7268 8
Size: 43
Fragments arranged by size:
2920 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4750 1537
Size: 275 2938 4090
Fragments arranged by size:
4090 2938 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3240 4386 5233 1353
Size: 1887 1146 847 3423
Fragments arranged by size:
3423 1887 1146 847
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3521 5890 6264 6410 387
Size: 1064 138 1932 2369 374 146 1280
Fragments arranged by size:
2369 1932 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2486 2901 2940 2979 3018 3135 3328 2486
Size: 415 39 39 39 117 193 6461
Fragments arranged by size:
6461 415 193 117 39 39 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2447 2531 2606 2711 2970 3909
Size: 393 480 335 84 75 105 259 939
Cuts at: 3909 4269 5366 5963 6750 7018 1239
Size: 360 1097 597 787 268 1524
Fragments arranged by size:
1524 1097 939 787 597 480 393 360
335 268 259 105 84 75
EcoNI CCTnn'n_nnAGG
Cuts at: 3612 4369 3612
Size: 757 6546
EcoO109I rG'GnC_Cy
Cuts at: 4587 4587
Size: 7303
EcoRI G'AATT_C
Cuts at: 3710 4121 4612 4990 3710
Size: 411 491 378 6023
Fragments arranged by size:
6023 491 411 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2657 2657
Size: 7303
FspI TGC'GCA
Cuts at: 5809 5809
Size: 7303
HaeII r_GCGC'y
Cuts at: 1678 6450 1678
Size: 4772 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 3557 3589 3609
Size: 32 135 32 770 32 1183 32 20
Cuts at: 3609 3623 3641 3655 5944 5976 6018 6050 1373
Size: 14 18 14 2289 32 42 32 2626
Fragments arranged by size:
2626 2289 1183 770 135 42 32 32
32 32 32 32 20 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2319 4647 4893 669
Size: 988 281 381 2328 246 3079
Fragments arranged by size:
3079 2328 988 381 281 246
HindIII A'AGCT_T
Cuts at: 748 4209 748
Size: 3461 3842
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KpnI G_GTAC'C
Cuts at: 2738 3933 4628 2738
Size: 1195 695 5413
Fragments arranged by size:
5413 1195 695
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 3374 7179 3374
Size: 3805 3498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6417 6601 1305
Size: 5112 184 2007
Fragments arranged by size:
5112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5481
Fragments arranged by size:
5481 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3546 5261 5620 5779 518
Size: 648 2380 1715 359 159 2042
Fragments arranged by size:
2380 2042 1715 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4719 5396 6544 6789 1606
Size: 3113 677 1148 245 2120
Fragments arranged by size:
3113 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6751
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 2767 4354 4661 6206 1132
Size: 97 1538 1587 307 1545 2229
Fragments arranged by size:
2229 1587 1545 1538 307 97
PasI CC'CwG_GG
Cuts at: 1348 2933 3050 3698 4472 1348
Size: 1585 117 648 774 4179
Fragments arranged by size:
4179 1585 774 648 117
PciI A'CATG_T
Cuts at: 6202 6202
Size: 7303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3203 1108
Size: 2095 5208
PfoI T'CCnGG_A
Cuts at: 2704 2704
Size: 7303
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 6885 6917 5372
Size: 32 1481 32 5758
Fragments arranged by size:
5758 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7303
PsiI TTA'TAA
Cuts at: 4873 4873
Size: 7303
PspXI vC'TCGA_Gb
Cuts at: 2814 2814
Size: 7303
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3016 3048 3016
Size: 32 7271
PstI C_TGCA'G
Cuts at: 830 2065 2210 2472 4655 830
Size: 1235 145 262 2183 3478
Fragments arranged by size:
3478 2183 1235 262 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
SacI G_AGCT'C
Cuts at: 2488 2903 2942 2981 3020 3137 3330 2488
Size: 415 39 39 39 117 193 6461
Fragments arranged by size:
6461 415 193 117 39 39 39
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 2917 2995 3034 3151 5551 7263 2917
Size: 78 39 117 2400 1712 2957
Fragments arranged by size:
2957 2400 1712 117 78 39
SexAI A'CCwGG_T
Cuts at: 2794 3064 2794
Size: 270 7033
SfcI C'TryA_G
Cuts at: 826 1045 2061 2206 2468 4651 5786 6467 6658
Size: 219 1016 145 262 2183 1135 681 191
Cuts at: 6658 826
Size: 1471
Fragments arranged by size:
2183 1471 1135 1016 681 262 219 191
145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 4630 1872
Size: 2758 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2814 5407 6308 6570 820
Size: 197 926 871 2593 901 262 1553
Fragments arranged by size:
2593 1553 926 901 871 262 197
SnaBI TAC'GTA
Cuts at: 493 5094 493
Size: 4601 2702
SpeI A'CTAG_T
Cuts at: 152 3755 152
Size: 3603 3700
SphI G_CATG'C
Cuts at: 1132 4661 1132
Size: 3529 3774
SspI AAT'ATT
Cuts at: 5 52 5227 5
Size: 47 5175 2081
Fragments arranged by size:
5175 2081 47
StuI AGG'CCT
Cuts at: 1517 3217 1517
Size: 1700 5603
StyI C'CwwG_G
Cuts at: 513 1065 2114 4490 4742 513
Size: 552 1049 2376 252 3074
Fragments arranged by size:
3074 2376 1049 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5330 5347 5500 5685 6130 325
Size: 1255 3750 17 153 185 445 1498
Fragments arranged by size:
3750 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2449 2915 2993
Size: 274 80 33 51 1443 472 466 78
Cuts at: 2993 3032 3149 4332 5549 7261 96
Size: 39 117 1183 1217 1712 138
Fragments arranged by size:
1712 1443 1217 1183 472 466 274 138
117 80 78 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 7263 7295 2088
Size: 32 5143 32 2096
Fragments arranged by size:
5143 2096 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3637 1316
Size: 2321 4982
XbaI T'CTAG_A
Cuts at: 2648 4639 2648
Size: 1991 5312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2329
Size: 7303
XhoI C'TCGA_G
Cuts at: 1943 2814 1943
Size: 871 6432
XmaI C'CCGG_G
Cuts at: 1870 4628 1870
Size: 2758 4545
XmnI GAAnn'nnTTC
Cuts at: 5432 5432
Size: 7303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI