Restriction map for
pFN21AB8507
(Circular) (Six-base) MAPSORT of: pFN21AB8507.seq Check: 9909 from: 1 to: 7528
pFN21AB8507 7528 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2110 4589 2110
Size: 2479 5049
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3990 5338 278
Size: 53 83 186 936 2454 1348 2468
Fragments arranged by size:
2468 2454 1348 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2769 4871 1937
Size: 832 2102 4594
Fragments arranged by size:
4594 2102 832
Acc65I G'GTAC_C
Cuts at: 3546 4389 4849 3546
Size: 843 460 6225
Fragments arranged by size:
6225 843 460
AclI AA'CG_TT
Cuts at: 1563 5655 6028 1563
Size: 4092 373 3063
Fragments arranged by size:
4092 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 3572 3716 3986 5591 6975 7243 1632
Size: 671 1269 144 270 1605 1384 268 1917
Fragments arranged by size:
1917 1605 1384 1269 671 270 268 144
AfeI AGC'GCT
Cuts at: 3644 3644
Size: 7528
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7331
AflIII A'CryG_T
Cuts at: 1203 6427 7404 1203
Size: 5224 977 1327
Fragments arranged by size:
5224 1327 977
AgeI A'CCGG_T
Cuts at: 6359 6359
Size: 7528
AhdI GACnn_n'nnGTC
Cuts at: 6257 6257
Size: 7528
AleI CACnn'nnGTG
Cuts at: 1166 3837 1166
Size: 2671 4857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7528
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2624 2656 2726 2758 2624
Size: 32 70 32 7394
Fragments arranged by size:
7394 70 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2737 3148 3331 3694 4282 4476 5271 6843 2737
Size: 411 183 363 588 194 795 1572 3422
Fragments arranged by size:
3422 1572 795 588 411 363 194 183
ApaI G_GGCC'C
Cuts at: 2799 3910 4624 2799
Size: 1111 714 5703
Fragments arranged by size:
5703 1111 714
ApaLI G'TGCA_C
Cuts at: 5585 6741 5585
Size: 1156 6372
ApoI r'AATT_y
Cuts at: 1493 4837 5063 5215 7324 1493
Size: 3344 226 152 2109 1697
Fragments arranged by size:
3344 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6082 160
Size: 872 5050 1606
Fragments arranged by size:
5050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2520 4853 1870
Size: 73 147 430 2333 4545
Fragments arranged by size:
4545 2333 430 147 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2258 2291 2415 2448 2258
Size: 33 124 33 7338
Fragments arranged by size:
7338 124 33 33
BamHI G'GATC_C
Cuts at: 2111 3919 4858 5225 2111
Size: 1808 939 367 4414
Fragments arranged by size:
4414 1808 939 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3546 4389 4849 6304 618
Size: 325 217 627 1759 843 460 1455 1842
Fragments arranged by size:
1842 1759 1455 843 627 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2799 2921 3020 3910 4434 4624 4847 1391
Size: 1408 122 99 890 524 190 223 4072
Fragments arranged by size:
4072 1408 890 524 223 190 122 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3573 928
Size: 971 1674 4883
Fragments arranged by size:
4883 1674 971
BbvCI CC'TCA_GC
Cuts at: 4175 4175
Size: 7528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5719 5753 1903
Size: 34 3782 34 3678
Fragments arranged by size:
3782 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5420 6630 4421
Size: 999 1210 5319
Fragments arranged by size:
5319 1210 999
BclI T'GATC_A
Cuts at: 1548 4648 1548
Size: 3100 4428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3732 6139
Size: 107 122 71 631 605 337 1723 2407
Cuts at: 6139 136
Size: 1525
Fragments arranged by size:
2407 1723 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2205 2346 4639 7523 2205
Size: 141 2293 2884 2210
Fragments arranged by size:
2884 2293 2210 141
BlpI GC'TnA_GC
Cuts at: 2069 2710 4945 2069
Size: 641 2235 4652
Fragments arranged by size:
4652 2235 641
Bme1580I G_kGCm'C
Cuts at: 1790 2799 3910 4624 4826 5589 6745 1790
Size: 1009 1111 714 202 763 1156 2573
Fragments arranged by size:
2573 1156 1111 1009 763 714 202
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3913 5259 6217
Size: 416 53 83 399 24 2496 1346 958
Cuts at: 6217 442
Size: 1753
Fragments arranged by size:
2496 1753 1346 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3212 4516 4570 3034
Size: 178 1304 54 5992
Fragments arranged by size:
5992 1304 178 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 3107 3434 4756 4798 4799 6188 1239
Size: 1139 729 327 1322 42 1 1389 2579
Fragments arranged by size:
2579 1389 1322 1139 729 327 42 1
Bpu10I CC'TnA_GC
Cuts at: 4175 4667 4175
Size: 492 7036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3380 3905 4214 4418 5653 6518 6816 3380
Size: 525 309 204 1235 865 298 4092
Fragments arranged by size:
4092 1235 865 525 309 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 4394 6191 882
Size: 617 1684 1211 1797 2219
Fragments arranged by size:
2219 1797 1684 1211 617
BsaAI yAC'GTr
Cuts at: 493 1204 3028 3271 5319 493
Size: 711 1824 243 2048 2702
Fragments arranged by size:
2702 2048 1824 711 243
BsaBI GATnn'nnATC
Cuts at: 7522 7522
Size: 7528
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3987 5335 5717 6352
Size: 53 83 186 936 2454 1348 382 635
Cuts at: 6352 275
Size: 1451
Fragments arranged by size:
2454 1451 1348 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2871 4596 5961 6359 6633 6780 7191 2871
Size: 1725 1365 398 274 147 411 3208
Fragments arranged by size:
3208 1725 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3080 3110 4626 4656 4735
Size: 30 165 30 1677 30 1516 30 79
Cuts at: 4735 4765 1178
Size: 30 3941
Fragments arranged by size:
3941 1677 1516 165 79 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3365 3431 3791 4211 4412 1186
Size: 2179 66 360 420 201 4302
Fragments arranged by size:
4302 2179 420 360 201 66
BseYI C'CCAG_C
Cuts at: 3294 3722 4784 6731 7227 3294
Size: 428 1062 1947 496 3595
Fragments arranged by size:
3595 1947 1062 496 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2371 3317 3866 4295 4489 1163
Size: 573 635 946 549 429 194 4202
Fragments arranged by size:
4202 946 635 573 549 429 194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4978 5739 6767 1540
Size: 275 186 2977 761 1028 2301
Fragments arranged by size:
2977 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2921 3834 4434 4676 5589 5674 6745 2921
Size: 913 600 242 913 85 1071 3704
Fragments arranged by size:
3704 1071 913 913 600 242 85
BsmI GAATG_Cn'
Cuts at: 3300 5039 5132 3300
Size: 1739 93 5696
Fragments arranged by size:
5696 1739 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2799 2921 3020 3834 3910 4434 4624
Size: 399 1009 122 99 814 76 524 190
Cuts at: 4624 4676 4826 4847 5589 5674 6745 1391
Size: 52 150 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 1009 814 742 524 399 190
150 122 99 85 76 52 21
BspEI T'CCGG_A
Cuts at: 7191 7191
Size: 7528
BspHI T'CATG_A
Cuts at: 3538 5417 7147 3538
Size: 1879 1730 3919
Fragments arranged by size:
3919 1879 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2110 2745 3330 4105 4589 4883 844
Size: 1181 85 635 585 775 484 294 3489
Fragments arranged by size:
3489 1181 775 635 585 484 294 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 5415 1394
Size: 429 264 3328 3507
Fragments arranged by size:
3507 3328 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 2080 2826 6023 6197 65
Size: 1183 832 746 3197 174 1396
Fragments arranged by size:
3197 1396 1183 832 746 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2526 4110 6172 6359 1700
Size: 110 716 1584 2062 187 2869
Fragments arranged by size:
2869 2062 1584 716 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7528
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7528
BssSI C'ACGA_G
Cuts at: 3911 4100 5588 6600 3911
Size: 189 1488 1012 4839
Fragments arranged by size:
4839 1488 1012 189
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3331 4081 794
Size: 2537 750 4241
Fragments arranged by size:
4241 2537 750
BstBI TT'CG_AA
Cuts at: 6332 6332
Size: 7528
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7528
BstXI CCAn_nnnn'nTGG
Cuts at: 2696 2696
Size: 7528
BstYI r'GATC_y
Cuts at: 1972 2111 2205 2346 3555 3654 3867 3919 4422
Size: 139 94 141 1209 99 213 52 503
Cuts at: 4422 4639 4858 5225 5610 5627 7079 7165 7177
Size: 217 219 367 385 17 1452 86 12
Cuts at: 7177 7523 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1209 503 385 367 346 219
217 213 141 139 99 94 86 52
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2689 4138 513
Size: 552 939 685 1449 3903
Fragments arranged by size:
3903 1449 939 685 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6789
Fragments arranged by size:
6789 683 56
BtsI GCAGTG_nn'
Cuts at: 2027 3056 4537 5044 5838 5858 2027
Size: 1029 1481 507 794 20 3697
Fragments arranged by size:
3697 1481 1029 794 507 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7493
DraI TTT'AAA
Cuts at: 4832 4832
Size: 7528
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4328 6535 809
Size: 507 3012 2207 1802
Fragments arranged by size:
3012 2207 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 2751 3348
Size: 54 1425 50 275 18 639 282 597
Cuts at: 3348 4519 4975 5864 7493 8
Size: 1171 456 889 1629 43
Fragments arranged by size:
1629 1425 1171 889 639 597 456 282
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4975 1537
Size: 275 3163 4090
Fragments arranged by size:
4090 3163 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2357 3962 4269 5458 1353
Size: 963 41 1605 307 1189 3423
Fragments arranged by size:
3423 1605 1189 963 307 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3536 4403 6115 6489 6635 387
Size: 1064 138 1947 867 1712 374 146 1280
Fragments arranged by size:
1947 1712 1280 1064 867 374 146 138
EcoICRI GAG'CTC
Cuts at: 2919 4432 2919
Size: 1513 6015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2378 3107 3434 3572 3716 3986
Size: 393 671 75 729 327 138 144 270
Cuts at: 3986 4756 4798 4799 5591 6188 6975 7243 1239
Size: 770 42 1 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 770 729 671 597 393
327 270 268 144 138 75 42 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 7528
EcoO109I rG'GnC_Cy
Cuts at: 2734 2796 3478 3751 4000 4150 4171 2734
Size: 62 682 273 249 150 21 6091
Fragments arranged by size:
6091 682 273 249 150 62 21
EcoRI G'AATT_C
Cuts at: 4837 5215 4837
Size: 378 7150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2742 3956 2742
Size: 1214 6314
FspI TGC'GCA
Cuts at: 6034 6034
Size: 7528
HaeII r_GCGC'y
Cuts at: 1678 2032 3646 4138 6675 1678
Size: 354 1614 492 2537 2531
Fragments arranged by size:
2537 2531 1614 492 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3130 3162 4735 4767 6169
Size: 32 135 32 1558 32 1573 32 1402
Cuts at: 6169 6201 6243 6275 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1573 1558 1402 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4872 5118 669
Size: 988 281 2934 246 3079
Fragments arranged by size:
3079 2934 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3938 748
Size: 3190 4338
HpaI GTT'AAC
Cuts at: 5118 5118
Size: 7528
KpnI G_GTAC'C
Cuts at: 3550 4393 4853 3550
Size: 843 460 6225
Fragments arranged by size:
6225 843 460
MfeI C'AATT_G
Cuts at: 5127 5127
Size: 7528
MluI A'CGCG_T
Cuts at: 7404 7404
Size: 7528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 2661 2946 3237 6642 6826 1305
Size: 985 371 285 291 3405 184 2007
Fragments arranged by size:
3405 2007 985 371 291 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 3350 4521 10
Size: 54 1425 343 639 879 1171 3017
Fragments arranged by size:
3017 1425 1171 879 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2694 3456 3837 4124 4610 5486 5845
Size: 648 1528 762 381 287 486 876 359
Cuts at: 5845 6004 518
Size: 159 2042
Fragments arranged by size:
2042 1528 876 762 648 486 381 359
287 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2117 4223 4944 5621 6769 7014 1606
Size: 462 49 2106 721 677 1148 245 2120
Fragments arranged by size:
2120 2106 1148 721 677 462 245 49
NcoI C'CATG_G
Cuts at: 513 1065 2004 2689 4138 513
Size: 552 939 685 1449 3903
Fragments arranged by size:
3903 1449 939 685 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7528
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7528
NotI GC'GGCC_GC
Cuts at: 4975 4975
Size: 7528
NspI r_CATG'y
Cuts at: 1132 1229 4096 4886 6431 1132
Size: 97 2867 790 1545 2229
Fragments arranged by size:
2867 2229 1545 790 97
PasI CC'CwG_GG
Cuts at: 1348 3862 1348
Size: 2514 5014
PciI A'CATG_T
Cuts at: 6427 6427
Size: 7528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3148 1108
Size: 2040 5488
PfoI T'CCnGG_A
Cuts at: 2410 4029 4814 2410
Size: 1619 785 5124
Fragments arranged by size:
5124 1619 785
PmeI GTTT'AAAC
Cuts at: 4832 4832
Size: 7528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5597 5629 7110 7142 5597
Size: 32 1481 32 5983
Fragments arranged by size:
5983 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2734 3478 4150 2734
Size: 744 672 6112
Fragments arranged by size:
6112 744 672
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7528
PsiI TTA'TAA
Cuts at: 5098 5098
Size: 7528
PspOMI G'GGCC_C
Cuts at: 2795 3906 4620 2795
Size: 1111 714 5703
Fragments arranged by size:
5703 1111 714
PstI C_TGCA'G
Cuts at: 830 2022 2742 3063 3327 3764 3900 4880 830
Size: 1192 720 321 264 437 136 980 3478
Fragments arranged by size:
3478 1192 980 720 437 321 264 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7528
PvuII CAG'CTG
Cuts at: 4223 4223
Size: 7528
SacI G_AGCT'C
Cuts at: 2921 4434 2921
Size: 1513 6015
SalI G'TCGA_C
Cuts at: 1936 4870 1936
Size: 2934 4594
SbfI CC_TGCA'GG
Cuts at: 4880 4880
Size: 7528
ScaI AGT'ACT
Cuts at: 5776 7488 5776
Size: 1712 5816
SexAI A'CCwGG_T
Cuts at: 4326 4326
Size: 7528
SfcI C'TryA_G
Cuts at: 826 1045 2018 2228 2738 2971 3059 3199 3323
Size: 219 973 210 510 233 88 140 124
Cuts at: 3323 3760 3896 4340 4876 6011 6692 6883 826
Size: 437 136 444 536 1135 681 191 1471
Fragments arranged by size:
1471 1135 973 681 536 510 444 437
233 219 210 191 140 136 124 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7528
SmaI CCC'GGG
Cuts at: 1872 2092 2522 4855 1872
Size: 220 430 2333 4545
Fragments arranged by size:
4545 2333 430 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 3884 4229 4433 5632 6533
Size: 197 926 1452 489 345 204 1199 901
Cuts at: 6533 6795 820
Size: 262 1553
Fragments arranged by size:
1553 1452 1199 926 901 489 345 262
204 197
SnaBI TAC'GTA
Cuts at: 493 5319 493
Size: 4826 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7528
SphI G_CATG'C
Cuts at: 1132 4886 1132
Size: 3754 3774
SspI AAT'ATT
Cuts at: 5 52 2296 5452 5
Size: 47 2244 3156 2081
Fragments arranged by size:
3156 2244 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2689 4138 4488 4967 513
Size: 552 939 333 352 1449 350 479 3074
Fragments arranged by size:
3074 1449 939 552 479 352 350 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 4760 5555 5572 5725 5910 6355
Size: 1255 1122 2058 795 17 153 185 445
Cuts at: 6355 325
Size: 1498
Fragments arranged by size:
2058 1498 1255 1122 795 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5774 7486
Size: 274 80 33 51 1443 1730 2067 1712
Cuts at: 7486 96
Size: 138
Fragments arranged by size:
2067 1730 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 7488 7520 3326
Size: 32 4130 32 3334
Fragments arranged by size:
4130 3334 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7528
XbaI T'CTAG_A
Cuts at: 4864 4864
Size: 7528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3253 4514 3253
Size: 1261 6267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7528
XmaI C'CCGG_G
Cuts at: 1870 2090 2520 4853 1870
Size: 220 430 2333 4545
Fragments arranged by size:
4545 2333 430 220
XmnI GAAnn'nnTTC
Cuts at: 3591 3821 4067 5657 3591
Size: 230 246 1590 5462
Fragments arranged by size:
5462 1590 246 230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3988 5336 276
Size: 53 83 186 936 2454 1348 2468
Fragments arranged by size:
2468 2454 1348 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI