Restriction map for
pFN21AB8502
(Circular) (Six-base) MAPSORT of: pFN21AB8502.seq Check: 9371 from: 1 to: 7297
pFN21AB8502 7297 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2807 3254 2807
Size: 447 6850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2840 5107 278
Size: 53 83 186 936 1304 2267 2468
Fragments arranged by size:
2468 2267 1304 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4640 1937
Size: 2703 4594
Acc65I G'GTAC_C
Cuts at: 4618 4618
Size: 7297
AclI AA'CG_TT
Cuts at: 1563 5424 5797 1563
Size: 3861 373 3063
Fragments arranged by size:
3861 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2997 5360 6744 7012 1632
Size: 1365 2363 1384 268 1917
Fragments arranged by size:
2363 1917 1384 1365 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7100
AflIII A'CryG_T
Cuts at: 1203 6196 7173 1203
Size: 4993 977 1327
Fragments arranged by size:
4993 1327 977
AgeI A'CCGG_T
Cuts at: 6128 6128
Size: 7297
AhdI GACnn_n'nnGTC
Cuts at: 6026 6026
Size: 7297
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3272 1184
Size: 2088 5209
AlwNI CAG_nnn'CTG
Cuts at: 2026 2362 3020 3094 3153 3373 4256 4471 5040
Size: 336 658 74 59 220 883 215 569
Cuts at: 5040 6612 2026
Size: 1572 2711
Fragments arranged by size:
2711 1572 883 658 569 336 220 215
74 59
ApaI G_GGCC'C
Cuts at: 2247 2351 4395 2247
Size: 104 2044 5149
Fragments arranged by size:
5149 2044 104
ApaLI G'TGCA_C
Cuts at: 5354 6510 5354
Size: 1156 6141
ApoI r'AATT_y
Cuts at: 1493 2182 4366 4606 4832 4984 7093 1493
Size: 689 2184 240 226 152 2109 1697
Fragments arranged by size:
2184 2109 1697 689 240 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5851 160
Size: 872 4819 1606
Fragments arranged by size:
4819 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7297
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2127 2459 2543 2553 2599 2759
Size: 73 125 59 332 84 10 46 160
Cuts at: 2759 2789 4622 1870
Size: 30 1833 4545
Fragments arranged by size:
4545 1833 332 160 125 84 73 59
46 30 10
AvrII C'CTAG_G
Cuts at: 4238 4238
Size: 7297
BamHI G'GATC_C
Cuts at: 4627 4994 4627
Size: 367 6930
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2192 2396 2642 2785
Size: 325 217 627 261 144 204 246 143
Cuts at: 2785 3135 3560 4618 6073 618
Size: 350 425 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 425 350 325 261
246 217 204 144 143
BanII G_rGCy'C
Cuts at: 1391 2247 2351 2848 3081 3189 3398 3550 3741
Size: 856 104 497 233 108 209 152 191
Cuts at: 3741 4302 4395 4616 1391
Size: 561 93 221 4072
Fragments arranged by size:
4072 856 561 497 233 221 209 191
152 108 104 93
BbeI G_GCGC'C
Cuts at: 2052 2400 2646 2789 2052
Size: 348 246 143 6560
Fragments arranged by size:
6560 348 246 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2422 928
Size: 971 523 5803
Fragments arranged by size:
5803 971 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5488 5522 1903
Size: 34 3551 34 3678
Fragments arranged by size:
3678 3551 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3064 5189 6399 3064
Size: 2125 1210 3962
Fragments arranged by size:
3962 2125 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7297
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3216 4512 5908
Size: 107 122 71 631 605 1544 1296 1396
Cuts at: 5908 136
Size: 1525
Fragments arranged by size:
1544 1525 1396 1296 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4149 4433 7292 4149
Size: 284 2859 4154
Fragments arranged by size:
4154 2859 284
BlpI GC'TnA_GC
Cuts at: 3410 3740 3956 4714 3410
Size: 330 216 758 5993
Fragments arranged by size:
5993 758 330 216
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2247 2351 3212 4395 5358 6514 1790
Size: 405 52 104 861 1183 963 1156 2573
Fragments arranged by size:
2573 1183 1156 963 861 405 104 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3478 4055 4169
Size: 416 53 83 399 24 2061 577 114
Cuts at: 4169 4270 5028 5986 442
Size: 101 758 958 1753
Fragments arranged by size:
2061 1753 958 758 577 416 399 114
101 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3230 1056
Size: 2174 5123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2794 4171 2188
Size: 606 1377 5314
Fragments arranged by size:
5314 1377 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2789 3173 3595 3807 3926 4112 5957 1239
Size: 1550 384 422 212 119 186 1845 2579
Fragments arranged by size:
2579 1845 1550 422 384 212 186 119
Bpu10I CC'TnA_GC
Cuts at: 3399 4296 3399
Size: 897 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 4520 5422 6287 6585 3425
Size: 1095 902 865 298 4137
Fragments arranged by size:
4137 1095 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2462 5960 882
Size: 617 963 3498 2219
Fragments arranged by size:
3498 2219 963 617
BsaAI yAC'GTr
Cuts at: 493 1204 5088 493
Size: 711 3884 2702
Fragments arranged by size:
3884 2702 711
BsaBI GATnn'nnATC
Cuts at: 7291 7291
Size: 7297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2397 2427 2502
Size: 53 83 186 936 516 348 30 75
Cuts at: 2502 2643 2786 2837 5104 5486 6121 275
Size: 141 143 51 2267 382 635 1451
Fragments arranged by size:
2267 1451 936 635 516 382 348 186
143 141 83 75 53 51 30
BsaWI w'CCGG_w
Cuts at: 5730 6128 6402 6549 6960 5730
Size: 398 274 147 411 6067
Fragments arranged by size:
6067 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 3303 3333 1178
Size: 30 165 30 1053 30 817 30 5142
Fragments arranged by size:
5142 1053 817 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4233 4418 1186
Size: 3047 185 4065
Fragments arranged by size:
4065 3047 185
BseYI C'CCAG_C
Cuts at: 2013 2120 2613 2928 3068 3523 3971 4387 4423
Size: 107 493 315 140 455 448 416 36
Cuts at: 4423 4494 6500 6996 2013
Size: 71 2006 496 2314
Fragments arranged by size:
2314 2006 496 493 455 448 416 315
140 107 71 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2953 3242 4327 1163
Size: 573 612 605 289 1085 4133
Fragments arranged by size:
4133 1085 612 605 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2236 2700 2713 2866 2940 4747
Size: 275 186 235 464 13 153 74 1807
Cuts at: 4747 5508 6536 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 464 275 235 186
153 74 13
BsiHKAI G_wGCw'C
Cuts at: 2571 3081 3189 4302 5358 5443 6514 2571
Size: 510 108 1113 1056 85 1071 3354
Fragments arranged by size:
3354 1113 1071 1056 510 108 85
BsiWI C'GTAC_G
Cuts at: 2271 2271
Size: 7297
BsmI GAATG_Cn'
Cuts at: 3206 4808 4901 3206
Size: 1602 93 5602
Fragments arranged by size:
5602 1602 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2440 2440
Size: 7297
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2247 2351 2571 2848 3081 3189
Size: 399 405 52 104 220 277 233 108
Cuts at: 3189 3212 3398 3550 3741 4302 4395 4616 5358
Size: 23 186 152 191 561 93 221 742
Cuts at: 5358 5443 6514 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 405 399 277 233
221 220 191 186 152 108 104 93
85 52 23
BspEI T'CCGG_A
Cuts at: 6960 6960
Size: 7297
BspHI T'CATG_A
Cuts at: 4269 5186 6916 4269
Size: 917 1730 4650
Fragments arranged by size:
4650 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2228 2807 3254 3264 3613 3870 4652 844
Size: 1384 579 447 10 349 257 782 3489
Fragments arranged by size:
3489 1384 782 579 447 349 257 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2778 2796 2868 5184 1394
Size: 429 955 18 72 2316 3507
Fragments arranged by size:
3507 2316 955 429 72 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 5792 5966 65
Size: 1183 4544 174 1396
Fragments arranged by size:
4544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 2861 2939 5941 6128 1700
Size: 110 425 626 78 3002 187 2869
Fragments arranged by size:
3002 2869 626 425 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 2472 96
Size: 2376 4921
BssHII G'CGCG_C
Cuts at: 1925 2665 1925
Size: 740 6557
BssSI C'ACGA_G
Cuts at: 4008 4289 5357 6369 4008
Size: 281 1068 1012 4936
Fragments arranged by size:
4936 1068 1012 281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3153 3373 794
Size: 2359 220 4718
Fragments arranged by size:
4718 2359 220
BstBI TT'CG_AA
Cuts at: 3421 6101 3421
Size: 2680 4617
BstEII G'GTnAC_C
Cuts at: 1184 2293 1184
Size: 1109 6188
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 3979 4068 4324 2290
Size: 1689 89 256 5263
Fragments arranged by size:
5263 1689 256 89
BstYI r'GATC_y
Cuts at: 1972 3126 4149 4433 4627 4994 5379 5396 6848
Size: 1154 1023 284 194 367 385 17 1452
Cuts at: 6848 6934 6946 7292 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1154 1023 385 367 346 284
194 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2723 3660 3746 3809 2723
Size: 937 86 63 6211
Fragments arranged by size:
6211 937 86 63
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2298 2382 2729 2934 3112
Size: 552 939 39 255 84 347 205 178
Cuts at: 3112 3556 513
Size: 444 4254
Fragments arranged by size:
4254 939 552 444 347 255 205 178
84 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2591 487
Size: 683 56 1365 5193
Fragments arranged by size:
5193 1365 683 56
BtsI GCAGTG_nn'
Cuts at: 2870 2970 3151 4813 5607 5627 2870
Size: 100 181 1662 794 20 4540
Fragments arranged by size:
4540 1662 794 181 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7262
DraI TTT'AAA
Cuts at: 4601 4601
Size: 7297
DraIII CAC_nnn'GTG
Cuts at: 2261 3506 2261
Size: 1245 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2138 6304 809
Size: 507 822 4166 1802
Fragments arranged by size:
4166 1802 822 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2353 2697
Size: 54 1425 50 275 18 403 120 344
Cuts at: 2697 2863 2893 2937 3821 3981 4744 5633 7262
Size: 166 30 44 884 160 763 889 1629
Cuts at: 7262 8
Size: 43
Fragments arranged by size:
1629 1425 889 884 763 403 344 275
166 160 120 54 50 44 43 30
18
EagI C'GGCC_G
Cuts at: 1537 1812 2233 2697 2863 2937 4744 1537
Size: 275 421 464 166 74 1807 4090
Fragments arranged by size:
4090 1807 464 421 275 166 74
EarI CTCTTCn'nnn_
Cuts at: 1353 2559 3086 3794 5227 1353
Size: 1206 527 708 1433 3423
Fragments arranged by size:
3423 1433 1206 708 527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2900 2994 4292 5884 6258 6404
Size: 1064 138 1311 94 1298 1592 374 146
Cuts at: 6404 387
Size: 1280
Fragments arranged by size:
1592 1311 1298 1280 1064 374 146 138
94
EcoICRI GAG'CTC
Cuts at: 3079 3187 4300 3079
Size: 108 1113 6076
Fragments arranged by size:
6076 1113 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2789 2997 3173 3595 3807 3926 4112
Size: 393 1157 208 176 422 212 119 186
Cuts at: 4112 5360 5957 6744 7012 1239
Size: 1248 597 787 268 1524
Fragments arranged by size:
1524 1248 1157 787 597 422 393 268
212 208 186 176 119
EcoNI CCTnn'n_nnAGG
Cuts at: 2722 3683 2722
Size: 961 6336
EcoO109I rG'GnC_Cy
Cuts at: 2073 2131 2348 2596 2964 3272 3337 3370 3688
Size: 58 217 248 368 308 65 33 318
Cuts at: 3688 3859 4253 4351 4391 4555 2073
Size: 171 394 98 40 164 4815
Fragments arranged by size:
4815 394 368 318 308 248 217 171
164 98 65 58 40 33
EcoRI G'AATT_C
Cuts at: 2182 4366 4606 4984 2182
Size: 2184 240 378 4495
Fragments arranged by size:
4495 2184 378 240
EcoRV GAT'ATC
Cuts at: 4015 4015
Size: 7297
FseI GG_CCGG'CC
Cuts at: 2865 2865
Size: 7297
FspI TGC'GCA
Cuts at: 5803 5803
Size: 7297
HaeII r_GCGC'y
Cuts at: 1678 2052 2148 2400 2614 2646 2789 6444 1678
Size: 374 96 252 214 32 143 3655 2531
Fragments arranged by size:
3655 2531 374 252 214 143 96 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3038 3070 3802 3834 4109
Size: 32 135 32 1466 32 732 32 275
Cuts at: 4109 4141 4219 4251 5938 5970 6012 6044 1373
Size: 32 78 32 1687 32 42 32 2626
Fragments arranged by size:
2626 1687 1466 732 275 135 78 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4641 4887 669
Size: 988 281 2703 246 3079
Fragments arranged by size:
3079 2703 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3416 748
Size: 2668 4629
HpaI GTT'AAC
Cuts at: 4887 4887
Size: 7297
KasI G'GCGC_C
Cuts at: 2048 2396 2642 2785 2048
Size: 348 246 143 6560
Fragments arranged by size:
6560 348 246 143
KpnI G_GTAC'C
Cuts at: 4622 4622
Size: 7297
MfeI C'AATT_G
Cuts at: 4896 4896
Size: 7297
MluI A'CGCG_T
Cuts at: 7173 7173
Size: 7297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4195 4585 6411 6595 1305
Size: 2890 390 1826 184 2007
Fragments arranged by size:
2890 2007 1826 390 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2355 3983 10
Size: 54 1425 343 523 1628 3324
Fragments arranged by size:
3324 1628 1425 523 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3248 5255 5614 5773 518
Size: 648 1314 768 2007 359 159 2042
Fragments arranged by size:
2042 2007 1314 768 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2045 2110 2179 2232 2317 2332 2384
Size: 417 22 65 69 53 85 15 52
Cuts at: 2384 2731 2936 3193 4713 5390 6538 6783 1606
Size: 347 205 257 1520 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 417 347 257 245
205 85 69 65 53 52 22 15
NaeI GCC'GGC
Cuts at: 2237 2863 2941 2237
Size: 626 78 6593
Fragments arranged by size:
6593 626 78
NarI GG'CG_CC
Cuts at: 2049 2397 2643 2786 2049
Size: 348 246 143 6560
Fragments arranged by size:
6560 348 246 143
NcoI C'CATG_G
Cuts at: 513 1065 2004 3556 513
Size: 552 939 1552 4254
Fragments arranged by size:
4254 1552 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7297
NgoMIV G'CCGG_C
Cuts at: 2235 2861 2939 2235
Size: 626 78 6593
Fragments arranged by size:
6593 626 78
NheI G'CTAG_C
Cuts at: 1052 3226 1052
Size: 2174 5123
NotI GC'GGCC_GC
Cuts at: 4744 4744
Size: 7297
NspI r_CATG'y
Cuts at: 1132 1229 4171 4655 6200 1132
Size: 97 2942 484 1545 2229
Fragments arranged by size:
2942 2229 1545 484 97
PasI CC'CwG_GG
Cuts at: 1348 3550 3889 4250 1348
Size: 2202 339 361 4395
Fragments arranged by size:
4395 2202 361 339
PciI A'CATG_T
Cuts at: 6196 6196
Size: 7297
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3904 1108
Size: 2796 4501
PfoI T'CCnGG_A
Cuts at: 2509 4089 2509
Size: 1580 5717
PmeI GTTT'AAAC
Cuts at: 4601 4601
Size: 7297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5366 5398 6879 6911 5366
Size: 32 1481 32 5752
Fragments arranged by size:
5752 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2131 2596 3337 3859 4351 2073
Size: 58 465 741 522 492 5019
Fragments arranged by size:
5019 741 522 492 465 58
PshAI GACnn'nnGTC
Cuts at: 1613 2505 3612 1613
Size: 892 1107 5298
Fragments arranged by size:
5298 1107 892
PsiI TTA'TAA
Cuts at: 4867 4867
Size: 7297
PspOMI G'GGCC_C
Cuts at: 2243 2347 4391 2243
Size: 104 2044 5149
Fragments arranged by size:
5149 2044 104
PstI C_TGCA'G
Cuts at: 830 2877 3090 3149 3378 3624 4338 4649 830
Size: 2047 213 59 229 246 714 311 3478
Fragments arranged by size:
3478 2047 714 311 246 229 213 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7297
PvuII CAG'CTG
Cuts at: 2023 2317 2332 3193 2023
Size: 294 15 861 6127
Fragments arranged by size:
6127 861 294 15
SacI G_AGCT'C
Cuts at: 3081 3189 4302 3081
Size: 108 1113 6076
Fragments arranged by size:
6076 1113 108
SacII CC_GC'GG
Cuts at: 2046 2385 2732 2937 2046
Size: 339 347 205 6406
Fragments arranged by size:
6406 347 339 205
SalI G'TCGA_C
Cuts at: 1936 4639 1936
Size: 2703 4594
SanDI GG'GwC_CC
Cuts at: 2073 2131 2596 2073
Size: 58 465 6774
Fragments arranged by size:
6774 465 58
SapI GCTCTTCn'nnn_
Cuts at: 2559 3086 2559
Size: 527 6770
SbfI CC_TGCA'GG
Cuts at: 3378 3624 4649 3378
Size: 246 1025 6026
Fragments arranged by size:
6026 1025 246
ScaI AGT'ACT
Cuts at: 4340 5545 7257 4340
Size: 1205 1712 4380
Fragments arranged by size:
4380 1712 1205
SexAI A'CCwGG_T
Cuts at: 3502 3502
Size: 7297
SfcI C'TryA_G
Cuts at: 826 1045 2873 2995 3086 3145 3374 3620 4334
Size: 219 1828 122 91 59 229 246 714
Cuts at: 4334 4645 5780 6461 6652 826
Size: 311 1135 681 191 1471
Fragments arranged by size:
1828 1471 1135 714 681 311 246 229
219 191 122 91 59
SfoI GGC'GCC
Cuts at: 2050 2398 2644 2787 2050
Size: 348 246 143 6560
Fragments arranged by size:
6560 348 246 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7297
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7297
SmaI CCC'GGG
Cuts at: 1872 2070 2129 2601 2791 4624 1872
Size: 198 59 472 190 1833 4545
Fragments arranged by size:
4545 1833 472 198 190 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 3404 4535 5401 6302 6564
Size: 197 926 610 851 1131 866 901 262
Cuts at: 6564 820
Size: 1553
Fragments arranged by size:
1553 1131 926 901 866 851 610 262
197
SnaBI TAC'GTA
Cuts at: 493 5088 493
Size: 4595 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7297
SphI G_CATG'C
Cuts at: 1132 4655 1132
Size: 3523 3774
SspI AAT'ATT
Cuts at: 5 52 5221 5
Size: 47 5169 2081
Fragments arranged by size:
5169 2081 47
StuI AGG'CCT
Cuts at: 1517 2753 4085 1517
Size: 1236 1332 4729
Fragments arranged by size:
4729 1332 1236
StyI C'CwwG_G
Cuts at: 513 1065 2004 2923 3556 4238 4736 513
Size: 552 939 919 633 682 498 3074
Fragments arranged by size:
3074 939 919 682 633 552 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2444 2751 5324 5341 5494 5679 6124
Size: 1255 864 307 2573 17 153 185 445
Cuts at: 6124 325
Size: 1498
Fragments arranged by size:
2573 1498 1255 864 445 307 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 4338 5543
Size: 274 80 33 51 1443 495 1866 1205
Cuts at: 5543 7255 96
Size: 1712 138
Fragments arranged by size:
1866 1712 1443 1205 495 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3303 3335 7257 7289 3303
Size: 32 3922 32 3311
Fragments arranged by size:
3922 3311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4208 1316
Size: 2892 4405
XbaI T'CTAG_A
Cuts at: 4633 4633
Size: 7297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3470 3563 3470
Size: 93 7204
XhoI C'TCGA_G
Cuts at: 1943 2553 1943
Size: 610 6687
XmaI C'CCGG_G
Cuts at: 1870 2068 2127 2599 2789 4622 1870
Size: 198 59 472 190 1833 4545
Fragments arranged by size:
4545 1833 472 198 190 59
XmnI GAAnn'nnTTC
Cuts at: 3803 5426 3803
Size: 1623 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2838 5105 276
Size: 53 83 186 936 1304 2267 2468
Fragments arranged by size:
2468 2267 1304 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BstZ17I FalI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SfiI SrfI SwaI