Restriction map for
pFN21AB8500
(Circular) (Six-base) MAPSORT of: pFN21AB8500.seq Check: 8236 from: 1 to: 7291
pFN21AB8500 7291 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2083
Size: 7291
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4353 5101 278
Size: 53 83 186 936 2817 748 2468
Fragments arranged by size:
2817 2468 936 748 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 4634 1937
Size: 994 1703 4594
Fragments arranged by size:
4594 1703 994
Acc65I G'GTAC_C
Cuts at: 4612 4612
Size: 7291
AclI AA'CG_TT
Cuts at: 1563 4590 5418 5791 1563
Size: 3027 828 373 3063
Fragments arranged by size:
3063 3027 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3363 5354 6738 7006 1632
Size: 1731 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1731 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2177 820
Size: 197 1160 5934
Fragments arranged by size:
5934 1160 197
AflIII A'CryG_T
Cuts at: 1203 2937 3246 3598 3801 6190 7167 1203
Size: 1734 309 352 203 2389 977 1327
Fragments arranged by size:
2389 1734 1327 977 352 309 203
AgeI A'CCGG_T
Cuts at: 6122 6122
Size: 7291
AhdI GACnn_n'nnGTC
Cuts at: 6020 6020
Size: 7291
AleI CACnn'nnGTG
Cuts at: 1166 3503 1166
Size: 2337 4954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2926 4440 1184
Size: 1742 1514 4035
Fragments arranged by size:
4035 1742 1514
AlwNI CAG_nnn'CTG
Cuts at: 2036 3133 3546 4289 4437 5034 6606 2036
Size: 1097 413 743 148 597 1572 2721
Fragments arranged by size:
2721 1572 1097 743 597 413 148
ApaLI G'TGCA_C
Cuts at: 2349 5348 6504 2349
Size: 2999 1156 3136
Fragments arranged by size:
3136 2999 1156
ApoI r'AATT_y
Cuts at: 1493 2519 3030 3686 3753 4600 4826 4978 7087
Size: 1026 511 656 67 847 226 152 2109
Cuts at: 7087 1493
Size: 1697
Fragments arranged by size:
2109 1697 1026 847 656 511 226 152
67
AseI AT'TA_AT
Cuts at: 160 1032 3837 5845 160
Size: 872 2805 2008 1606
Fragments arranged by size:
2805 2008 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 3484 4407 4616 1870
Size: 73 80 1461 923 209 4545
Fragments arranged by size:
4545 1461 923 209 80 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2939 2972 2939
Size: 33 7258
BamHI G'GATC_C
Cuts at: 4621 4988 4621
Size: 367 6924
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 3383 4612 6067 618
Size: 325 217 627 366 1230 1229 1455 1842
Fragments arranged by size:
1842 1455 1230 1229 627 366 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2342 3341 3590 4610 1391
Size: 633 318 999 249 1020 4072
Fragments arranged by size:
4072 1020 999 633 318 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3360 928
Size: 971 1461 4859
Fragments arranged by size:
4859 1461 971
BbvCI CC'TCA_GC
Cuts at: 3702 3702
Size: 7291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4259 4293 5482 5516 1903
Size: 34 2322 34 1189 34 3678
Fragments arranged by size:
3678 2322 1189 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2967 5183 6393 2967
Size: 2216 1210 3865
Fragments arranged by size:
3865 2216 1210
BclI T'GATC_A
Cuts at: 1548 2416 4494 1548
Size: 868 2078 4345
Fragments arranged by size:
4345 2078 868
BfrBI ATG'CAT
Cuts at: 2573 3166 2573
Size: 593 6698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5902 136
Size: 107 122 71 631 605 4230 1525
Fragments arranged by size:
4230 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3410 7286 3410
Size: 3876 3415
BlpI GC'TnA_GC
Cuts at: 4373 4708 4373
Size: 335 6956
Bme1580I G_kGCm'C
Cuts at: 1790 2353 3492 3855 5352 6508 1790
Size: 563 1139 363 1497 1156 2573
Fragments arranged by size:
2573 1497 1156 1139 563 363
BmgBI CAC'GTC
Cuts at: 1318 3247 1318
Size: 1929 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 5022 5980
Size: 416 53 83 399 24 2024 1581 958
Cuts at: 5980 442
Size: 1753
Fragments arranged by size:
2024 1753 1581 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 7291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3000 3249 3306 3987 4142 4266 4414 5951
Size: 1761 249 57 681 155 124 148 1537
Cuts at: 5951 1239
Size: 2579
Fragments arranged by size:
2579 1761 1537 681 249 155 148 124
57
Bpu10I CC'TnA_GC
Cuts at: 2040 3702 2040
Size: 1662 5629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5416 6281 6579 5416
Size: 865 298 6128
Fragments arranged by size:
6128 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5954 882
Size: 617 4455 2219
Fragments arranged by size:
4455 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5082 493
Size: 711 3878 2702
Fragments arranged by size:
3878 2702 711
BsaBI GATnn'nnATC
Cuts at: 7285 7285
Size: 7291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3402 4350 5098 5480
Size: 53 83 186 936 1869 948 748 382
Cuts at: 5480 6115 275
Size: 635 1451
Fragments arranged by size:
1869 1451 948 936 748 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3677 5724 6122 6396 6543 6954 3677
Size: 2047 398 274 147 411 4014
Fragments arranged by size:
4014 2047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7066
Fragments arranged by size:
7066 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7291
BseYI C'CCAG_C
Cuts at: 2666 2899 6494 6990 2666
Size: 233 3595 496 2967
Fragments arranged by size:
3595 2967 496 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 1163
Size: 573 765 5953
Fragments arranged by size:
5953 765 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4741 5502 6530 1540
Size: 275 186 2740 761 1028 2301
Fragments arranged by size:
2740 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2342 2353 4158 5352 5437 6508 2024
Size: 318 11 1805 1194 85 1071 2807
Fragments arranged by size:
2807 1805 1194 1071 318 85 11
BsmI GAATG_Cn'
Cuts at: 2999 4802 4895 2999
Size: 1803 93 5395
Fragments arranged by size:
5395 1803 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2342 2353 3341 3492 3590 3855
Size: 399 234 318 11 988 151 98 265
Cuts at: 3855 4158 4610 5352 5437 6508 1391
Size: 303 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 988 742 452 399 318 303
265 234 151 98 85 11
BspEI T'CCGG_A
Cuts at: 6954 6954
Size: 7291
BspHI T'CATG_A
Cuts at: 3904 5180 6910 3904
Size: 1276 1730 4285
Fragments arranged by size:
4285 1730 1276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 2083 2384 4646 844
Size: 1200 39 301 2262 3489
Fragments arranged by size:
3489 2262 1200 301 39
BsrBI CCG'CTC
Cuts at: 1394 1823 3467 5178 1394
Size: 429 1644 1711 3507
Fragments arranged by size:
3507 1711 1644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5786 5960 65
Size: 1183 4538 174 1396
Fragments arranged by size:
4538 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2883 4398 5935 6122 1700
Size: 110 1073 1515 1537 187 2869
Fragments arranged by size:
2869 1537 1515 1073 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7291
BssSI C'ACGA_G
Cuts at: 2918 5351 6363 2918
Size: 2433 1012 3846
Fragments arranged by size:
3846 2433 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 3133 3546 4153 794
Size: 2240 99 413 607 3932
Fragments arranged by size:
3932 2240 607 413 99
BstBI TT'CG_AA
Cuts at: 2244 6095 2244
Size: 3851 3440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7291
BstYI r'GATC_y
Cuts at: 1972 2194 3410 4621 4988 5373 5390 6842 6928
Size: 222 1216 1211 367 385 17 1452 86
Cuts at: 6928 6940 7286 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1216 1211 385 367 346 222
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3189 3504 4110 4537 513
Size: 552 939 1185 315 606 427 3267
Fragments arranged by size:
3267 1185 939 606 552 427 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2373 4546 487
Size: 683 56 1147 2173 3232
Fragments arranged by size:
3232 2173 1147 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 3131 3432 3544 4807 5601 5621 2112
Size: 1019 301 112 1263 794 20 3782
Fragments arranged by size:
3782 1263 1019 794 301 112 20
ClaI AT'CG_AT
Cuts at: 1554 4269 1554
Size: 2715 4576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2146 2181 2203 2238 529
Size: 35 1582 35 22 35 5582
Fragments arranged by size:
5582 1582 35 35 35 22
DraI TTT'AAA
Cuts at: 3069 3742 4595 3069
Size: 673 853 5765
Fragments arranged by size:
5765 853 673
DraIII CAC_nnn'GTG
Cuts at: 2348 4153 2348
Size: 1805 5486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3401 6298 809
Size: 507 2085 2897 1802
Fragments arranged by size:
2897 2085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3564 4738 5627
Size: 54 1425 50 275 18 1734 1174 889
Cuts at: 5627 7256 8
Size: 1629 43
Fragments arranged by size:
1734 1629 1425 1174 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4738 1537
Size: 275 2926 4090
Fragments arranged by size:
4090 2926 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 3285 5221 1353
Size: 1404 528 1936 3423
Fragments arranged by size:
3423 1936 1404 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5878 6252 6398 387
Size: 1064 138 4289 374 146 1280
Fragments arranged by size:
4289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2340 2022
Size: 318 6973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3000 3249 3306 3363 3987 4142 4266
Size: 393 1368 249 57 57 624 155 124
Cuts at: 4266 4414 5354 5951 6738 7006 1239
Size: 148 940 597 787 268 1524
Fragments arranged by size:
1524 1368 940 787 624 597 393 268
249 155 148 124 57 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 7291
EcoRI G'AATT_C
Cuts at: 4600 4978 4600
Size: 378 6913
EcoRV GAT'ATC
Cuts at: 3004 4087 3004
Size: 1083 6208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3773 4144 4270 3773
Size: 371 126 6794
Fragments arranged by size:
6794 371 126
FspI TGC'GCA
Cuts at: 5797 5797
Size: 7291
HaeII r_GCGC'y
Cuts at: 1678 3623 6438 1678
Size: 1945 2815 2531
Fragments arranged by size:
2815 2531 1945
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2401 2433 3421
Size: 32 135 32 479 32 318 32 988
Cuts at: 3421 3453 3572 3604 4213 4245 5932 5964 6006
Size: 32 119 32 609 32 1687 32 42
Cuts at: 6006 6038 1373
Size: 32 2626
Fragments arranged by size:
2626 1687 988 609 479 318 135 119
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 4635 4881 669
Size: 988 281 1168 1529 246 3079
Fragments arranged by size:
3079 1529 1168 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2174 3050 748
Size: 1426 876 4989
Fragments arranged by size:
4989 1426 876
HpaI GTT'AAC
Cuts at: 4881 4881
Size: 7291
KpnI G_GTAC'C
Cuts at: 4616 4616
Size: 7291
MfeI C'AATT_G
Cuts at: 4890 4890
Size: 7291
MluI A'CGCG_T
Cuts at: 7167 7167
Size: 7291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 2824 6405 6589 1305
Size: 1072 447 3581 184 2007
Fragments arranged by size:
3581 2007 1072 447 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5469
Fragments arranged by size:
5469 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3503 4502 5249 5608 5767 518
Size: 648 2337 999 747 359 159 2042
Fragments arranged by size:
2337 2042 999 747 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2737 3173 4707 5384 6532 6777 1606
Size: 1131 436 1534 677 1148 245 2120
Fragments arranged by size:
2120 1534 1148 1131 677 436 245
NaeI GCC'GGC
Cuts at: 4400 4400
Size: 7291
NcoI C'CATG_G
Cuts at: 513 1065 2004 3189 4537 513
Size: 552 939 1185 1348 3267
Fragments arranged by size:
3267 1348 1185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7291
NgoMIV G'CCGG_C
Cuts at: 4398 4398
Size: 7291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7291
NotI GC'GGCC_GC
Cuts at: 4738 4738
Size: 7291
NsiI A_TGCA'T
Cuts at: 2575 3168 2575
Size: 593 6698
NspI r_CATG'y
Cuts at: 1132 1229 2573 2941 3170 3602 3805 4109 4649
Size: 97 1344 368 229 432 203 304 540
Cuts at: 4649 6194 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1344 540 432 368 304 229
203 97
PasI CC'CwG_GG
Cuts at: 1348 3575 4385 1348
Size: 2227 810 4254
Fragments arranged by size:
4254 2227 810
PciI A'CATG_T
Cuts at: 2937 3598 3801 6190 2937
Size: 661 203 2389 4038
Fragments arranged by size:
4038 2389 661 203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7291
PfoI T'CCnGG_A
Cuts at: 3964 3964
Size: 7291
PmeI GTTT'AAAC
Cuts at: 4595 4595
Size: 7291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 6873 6905 5360
Size: 32 1481 32 5746
Fragments arranged by size:
5746 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2471 3774 1613
Size: 858 1303 5130
Fragments arranged by size:
5130 1303 858
PsiI TTA'TAA
Cuts at: 4064 4861 4064
Size: 797 6494
PspXI vC'TCGA_Gb
Cuts at: 2023 2023
Size: 7291
PstI C_TGCA'G
Cuts at: 830 2915 3551 4143 4643 830
Size: 2085 636 592 500 3478
Fragments arranged by size:
3478 2085 636 592 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7291
PvuII CAG'CTG
Cuts at: 2737 2737
Size: 7291
SacI G_AGCT'C
Cuts at: 2024 2342 2024
Size: 318 6973
SalI G'TCGA_C
Cuts at: 1936 4633 1936
Size: 2697 4594
SbfI CC_TGCA'GG
Cuts at: 4643 4643
Size: 7291
ScaI AGT'ACT
Cuts at: 5539 7251 5539
Size: 1712 5579
SfcI C'TryA_G
Cuts at: 826 1045 2911 3547 4139 4639 5774 6455 6646
Size: 219 1866 636 592 500 1135 681 191
Cuts at: 6646 826
Size: 1471
Fragments arranged by size:
1866 1471 1135 681 636 592 500 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7291
SmaI CCC'GGG
Cuts at: 1872 4618 1872
Size: 2746 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2023 2177 5395 6296 6558 820
Size: 197 926 80 154 3218 901 262 1553
Fragments arranged by size:
3218 1553 926 901 262 197 154 80
SnaBI TAC'GTA
Cuts at: 493 5082 493
Size: 4589 2702
SpeI A'CTAG_T
Cuts at: 152 4319 152
Size: 4167 3124
SphI G_CATG'C
Cuts at: 1132 3170 4109 4649 1132
Size: 2038 939 540 3774
Fragments arranged by size:
3774 2038 939 540
SspI AAT'ATT
Cuts at: 5 52 2164 2533 4239 5215 5
Size: 47 2112 369 1706 976 2081
Fragments arranged by size:
2112 2081 1706 976 369 47
StuI AGG'CCT
Cuts at: 1517 3272 1517
Size: 1755 5536
StyI C'CwwG_G
Cuts at: 513 1065 2004 3189 4537 4730 513
Size: 552 939 1185 1348 193 3074
Fragments arranged by size:
3074 1348 1185 939 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4514 4562 5318 5335 5488 5673 6118
Size: 1255 2934 48 756 17 153 185 445
Cuts at: 6118 325
Size: 1498
Fragments arranged by size:
2934 1498 1255 756 445 185 153 48
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2027 5537 7249
Size: 274 80 33 51 1443 50 3510 1712
Cuts at: 7249 96
Size: 138
Fragments arranged by size:
3510 1712 1443 274 138 80 51 50
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7251 7283 7251
Size: 32 7259
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7291
XbaI T'CTAG_A
Cuts at: 4627 4627
Size: 7291
XhoI C'TCGA_G
Cuts at: 1943 2023 1943
Size: 80 7211
XmaI C'CCGG_G
Cuts at: 1870 4616 1870
Size: 2746 4545
XmnI GAAnn'nnTTC
Cuts at: 2851 3753 4215 5420 2851
Size: 902 462 1205 4722
Fragments arranged by size:
4722 1205 902 462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4351 5099 276
Size: 53 83 186 936 2817 748 2468
Fragments arranged by size:
2817 2468 936 748 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoO109I FseI FspAI KasI NarI
NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI