Restriction map for
pFN21AB8496
(Circular) (Six-base) MAPSORT of: pFN21AB8496.seq Check: 4862 from: 1 to: 7288
pFN21AB8496 7288 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5098 278
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4631 1937
Size: 2694 4594
Acc65I G'GTAC_C
Cuts at: 2434 4609 2434
Size: 2175 5113
AclI AA'CG_TT
Cuts at: 1563 5415 5788 1563
Size: 3852 373 3063
Fragments arranged by size:
3852 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 3024 5351 6735 7003 1632
Size: 416 976 2327 1384 268 1917
Fragments arranged by size:
2327 1917 1384 976 416 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7091
AflIII A'CryG_T
Cuts at: 1203 2623 4408 6187 7164 1203
Size: 1420 1785 1779 977 1327
Fragments arranged by size:
1785 1779 1420 1327 977
AgeI A'CCGG_T
Cuts at: 6119 6119
Size: 7288
AhdI GACnn_n'nnGTC
Cuts at: 6017 6017
Size: 7288
AleI CACnn'nnGTG
Cuts at: 1166 3371 4165 1166
Size: 2205 794 4289
Fragments arranged by size:
4289 2205 794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2575 3513 4183 1184
Size: 1391 938 670 4289
Fragments arranged by size:
4289 1391 938 670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3519 3551 3989 4021 4317 4349 3519
Size: 32 438 32 296 32 6458
Fragments arranged by size:
6458 438 296 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5031 6603 5031
Size: 1572 5716
ApaLI G'TGCA_C
Cuts at: 2328 5345 6501 2328
Size: 3017 1156 3115
Fragments arranged by size:
3115 3017 1156
ApoI r'AATT_y
Cuts at: 1493 2495 3533 4309 4597 4823 4975 7084 1493
Size: 1002 1038 776 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 1038 1002 776 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2376 5842 160
Size: 872 1344 3466 1606
Fragments arranged by size:
3466 1606 1344 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 3825 4613 1870
Size: 73 313 1569 788 4545
Fragments arranged by size:
4545 1569 788 313 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4424 4457 4424
Size: 33 7255
BamHI G'GATC_C
Cuts at: 4618 4985 4618
Size: 367 6921
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2434 3046 3373 4167 4609
Size: 325 217 627 647 612 327 794 442
Cuts at: 4609 6064 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 794 647 627 612 442 327
325 217
BanII G_rGCy'C
Cuts at: 1391 2603 3732 3870 4487 4512 4607 1391
Size: 1212 1129 138 617 25 95 4072
Fragments arranged by size:
4072 1212 1129 617 138 95 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 2465 3629 928
Size: 971 137 429 1164 4587
Fragments arranged by size:
4587 1164 971 429 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3170 3204 5479 5513 1903
Size: 34 1233 34 2275 34 3678
Fragments arranged by size:
3678 2275 1233 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 5180 6390 3149
Size: 2031 1210 4047
Fragments arranged by size:
4047 2031 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7288
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5899 136
Size: 107 122 71 631 605 4227 1525
Fragments arranged by size:
4227 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2834 3035 4197 7283 2834
Size: 201 1162 3086 2839
Fragments arranged by size:
3086 2839 1162 201
BlpI GC'TnA_GC
Cuts at: 4705 4705
Size: 7288
Bme1580I G_kGCm'C
Cuts at: 1790 2332 5349 6505 1790
Size: 542 3017 1156 2573
Fragments arranged by size:
3017 2573 1156 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7288
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4025 5019 5977
Size: 416 53 83 399 24 2608 994 958
Cuts at: 5977 442
Size: 1753
Fragments arranged by size:
2608 1753 994 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 4364 2836
Size: 1528 5760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 2763 2850 3488 4173 4394 5948 1239
Size: 1496 28 87 638 685 221 1554 2579
Fragments arranged by size:
2579 1554 1496 685 638 221 87 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 3374 4070 5413 6278 6576 2231
Size: 1143 696 1343 865 298 2943
Fragments arranged by size:
2943 1343 1143 865 696 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 4451 5951 882
Size: 617 879 2073 1500 2219
Fragments arranged by size:
2219 2073 1500 879 617
BsaAI yAC'GTr
Cuts at: 493 1204 5079 493
Size: 711 3875 2702
Fragments arranged by size:
3875 2702 711
BsaBI GATnn'nnATC
Cuts at: 2141 7282 2141
Size: 5141 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5095 5477 6112 275
Size: 53 83 186 936 3562 382 635 1451
Fragments arranged by size:
3562 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3581 3602 5721 6119 6393 6540 6951 3581
Size: 21 2119 398 274 147 411 3918
Fragments arranged by size:
3918 2119 411 398 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 2848 2878 3112
Size: 30 165 30 1239 30 176 30 234
Cuts at: 3112 3142 4469 4499 1178
Size: 30 1327 30 3967
Fragments arranged by size:
3967 1327 1239 234 176 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2229 3014 3408 1186
Size: 1043 785 394 5066
Fragments arranged by size:
5066 1043 785 394
BseYI C'CCAG_C
Cuts at: 2614 2748 4212 4511 6491 6987 2614
Size: 134 1464 299 1980 496 2915
Fragments arranged by size:
2915 1980 1464 496 299 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2571 2613 3805 4441 1163
Size: 573 330 505 42 1192 636 4010
Fragments arranged by size:
4010 1192 636 573 505 330 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4738 5499 6527 1540
Size: 275 186 2737 761 1028 2301
Fragments arranged by size:
2737 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2257 2332 3310 3870 5349 5434 6505 2257
Size: 75 978 560 1479 85 1071 3040
Fragments arranged by size:
3040 1479 1071 978 560 85 75
BsmI GAATG_Cn'
Cuts at: 4799 4892 4799
Size: 93 7195
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2332 2603 3310 3732 3870 4487
Size: 399 467 75 271 707 422 138 617
Cuts at: 4487 4512 4607 5349 5434 6505 1391
Size: 25 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 617 467 422 399
271 138 95 85 75 25
BspEI T'CCGG_A
Cuts at: 6951 6951
Size: 7288
BspHI T'CATG_A
Cuts at: 3637 3990 5177 6907 3637
Size: 353 1187 1730 4018
Fragments arranged by size:
4018 1730 1187 353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3339 3984 4643 844
Size: 2495 645 659 3489
Fragments arranged by size:
3489 2495 659 645
BsrBI CCG'CTC
Cuts at: 1394 1823 3416 5175 1394
Size: 429 1593 1759 3507
Fragments arranged by size:
3507 1759 1593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5783 5957 65
Size: 1183 4535 174 1396
Fragments arranged by size:
4535 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5932 6119 1700
Size: 110 4122 187 2869
Fragments arranged by size:
4122 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3594 3903 96
Size: 3498 309 3481
Fragments arranged by size:
3498 3481 309
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7288
BssSI C'ACGA_G
Cuts at: 5348 6360 5348
Size: 1012 6276
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3754 794
Size: 2960 4328
BstBI TT'CG_AA
Cuts at: 2220 2978 6092 2220
Size: 758 3114 3416
Fragments arranged by size:
3416 3114 758
BstEII G'GTnAC_C
Cuts at: 1184 2732 3831 4240 1184
Size: 1548 1099 409 4232
Fragments arranged by size:
4232 1548 1099 409
BstXI CCAn_nnnn'nTGG
Cuts at: 3466 3970 4015 4123 3466
Size: 504 45 108 6631
Fragments arranged by size:
6631 504 108 45
BstYI r'GATC_y
Cuts at: 1972 2834 3035 3423 4068 4197 4618 4985 5370
Size: 862 201 388 645 129 421 367 385
Cuts at: 5370 5387 6839 6925 6937 7283 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 862 645 421 388 385 367
346 201 129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3837 4073 3837
Size: 236 7052
BtgI C'CryG_G
Cuts at: 513 1065 2004 4461 513
Size: 552 939 2457 3340
Fragments arranged by size:
3340 2457 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2930 487
Size: 683 56 1704 4845
Fragments arranged by size:
4845 1704 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4804 5598 5618 3271
Size: 1533 794 20 4941
Fragments arranged by size:
4941 1533 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2412 2447 529
Size: 35 1848 35 5370
Fragments arranged by size:
5370 1848 35 35
DraI TTT'AAA
Cuts at: 4592 4592
Size: 7288
DraIII CAC_nnn'GTG
Cuts at: 3410 3410
Size: 7288
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6295 809
Size: 507 4979 1802
Fragments arranged by size:
4979 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 4299 4464
Size: 54 1425 50 275 18 798 1671 165
Cuts at: 4464 4735 5624 7253 8
Size: 271 889 1629 43
Fragments arranged by size:
1671 1629 1425 889 798 275 271 165
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4735 1537
Size: 275 2923 4090
Fragments arranged by size:
4090 2923 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3216 3270 3377 3519 5218 1353
Size: 1863 54 107 142 1699 3423
Fragments arranged by size:
3423 1863 1699 142 107 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2644 5875 6249 6395 387
Size: 1064 138 1055 3231 374 146 1280
Fragments arranged by size:
3231 1280 1064 1055 374 146 138
EcoICRI GAG'CTC
Cuts at: 3868 3868
Size: 7288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2735 2763 2850 3024 3488 4173
Size: 393 416 687 28 87 174 464 685
Cuts at: 4173 4394 5351 5948 6735 7003 1239
Size: 221 957 597 787 268 1524
Fragments arranged by size:
1524 957 787 687 685 597 464 416
393 268 221 174 87 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3368 3615 3659 3368
Size: 247 44 6997
Fragments arranged by size:
6997 247 44
EcoO109I rG'GnC_Cy
Cuts at: 2061 4077 2061
Size: 2016 5272
EcoRI G'AATT_C
Cuts at: 3533 4597 4975 3533
Size: 1064 378 5846
Fragments arranged by size:
5846 1064 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 3370 2482
Size: 888 6400
FspI TGC'GCA
Cuts at: 5794 5794
Size: 7288
HaeII r_GCGC'y
Cuts at: 1678 6435 1678
Size: 4757 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3112 3144 3154 3186 3415
Size: 32 135 32 1540 32 10 32 229
Cuts at: 3415 3447 4387 4419 5929 5961 6003 6035 1373
Size: 32 940 32 1510 32 42 32 2626
Fragments arranged by size:
2626 1540 1510 940 229 135 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 4406 4632 4878 669
Size: 988 281 2468 226 246 3079
Fragments arranged by size:
3079 2468 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7288
HpaI GTT'AAC
Cuts at: 4878 4878
Size: 7288
KpnI G_GTAC'C
Cuts at: 2438 4613 2438
Size: 2175 5113
MfeI C'AATT_G
Cuts at: 3957 4887 3957
Size: 930 6358
MluI A'CGCG_T
Cuts at: 7164 7164
Size: 7288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 6402 6586 1305
Size: 744 4353 184 2007
Fragments arranged by size:
4353 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 10
Size: 54 1425 343 798 4668
Fragments arranged by size:
4668 1425 798 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 4165 4466 5246 5605 5764 518
Size: 648 2205 794 301 780 359 159 2042
Fragments arranged by size:
2205 2042 794 780 648 359 301 159
MspA1I CmG'CkG
Cuts at: 1606 4060 4123 4704 5381 6529 6774 1606
Size: 2454 63 581 677 1148 245 2120
Fragments arranged by size:
2454 2120 1148 677 581 245 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 4461 513
Size: 552 939 2457 3340
Fragments arranged by size:
3340 2457 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7288
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7288
NotI GC'GGCC_GC
Cuts at: 4735 4735
Size: 7288
NspI r_CATG'y
Cuts at: 1132 1229 2627 3494 3595 4412 4646 6191 1132
Size: 97 1398 867 101 817 234 1545 2229
Fragments arranged by size:
2229 1545 1398 867 817 234 101 97
PasI CC'CwG_GG
Cuts at: 1348 3611 1348
Size: 2263 5025
PciI A'CATG_T
Cuts at: 2623 4408 6187 2623
Size: 1785 1779 3724
Fragments arranged by size:
3724 1785 1779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4258 1108
Size: 3150 4138
PfoI T'CCnGG_A
Cuts at: 2480 2779 3323 2480
Size: 299 544 6445
Fragments arranged by size:
6445 544 299
PmeI GTTT'AAAC
Cuts at: 4592 4592
Size: 7288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5357 5389 6870 6902 5357
Size: 32 1481 32 5743
Fragments arranged by size:
5743 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4077 4077
Size: 7288
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7288
PsiI TTA'TAA
Cuts at: 4858 4858
Size: 7288
PspXI vC'TCGA_Gb
Cuts at: 2256 2256
Size: 7288
PstI C_TGCA'G
Cuts at: 830 3660 4065 4184 4640 830
Size: 2830 405 119 456 3478
Fragments arranged by size:
3478 2830 456 405 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7288
PvuII CAG'CTG
Cuts at: 4060 4123 4060
Size: 63 7225
SacI G_AGCT'C
Cuts at: 3870 3870
Size: 7288
SalI G'TCGA_C
Cuts at: 1936 4630 1936
Size: 2694 4594
SbfI CC_TGCA'GG
Cuts at: 4640 4640
Size: 7288
ScaI AGT'ACT
Cuts at: 5536 7248 5536
Size: 1712 5576
SfcI C'TryA_G
Cuts at: 826 1045 2095 3656 4061 4180 4636 5771 6452
Size: 219 1050 1561 405 119 456 1135 681
Cuts at: 6452 6643 826
Size: 191 1471
Fragments arranged by size:
1561 1471 1135 1050 681 456 405 219
191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7288
SmaI CCC'GGG
Cuts at: 1872 4615 1872
Size: 2743 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2256 3389 4085 5392 6293
Size: 197 926 267 46 1133 696 1307 901
Cuts at: 6293 6555 820
Size: 262 1553
Fragments arranged by size:
1553 1307 1133 926 901 696 267 262
197 46
SnaBI TAC'GTA
Cuts at: 493 5079 493
Size: 4586 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7288
SphI G_CATG'C
Cuts at: 1132 4646 1132
Size: 3514 3774
SspI AAT'ATT
Cuts at: 5 52 2340 5212 5
Size: 47 2288 2872 2081
Fragments arranged by size:
2872 2288 2081 47
StuI AGG'CCT
Cuts at: 1517 4100 1517
Size: 2583 4705
StyI C'CwwG_G
Cuts at: 513 1065 2004 2769 3299 4461 4727 513
Size: 552 939 765 530 1162 266 3074
Fragments arranged by size:
3074 1162 939 765 552 530 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3325 5315 5332 5485 5670 6115 325
Size: 1255 1745 1990 17 153 185 445 1498
Fragments arranged by size:
1990 1745 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 3594 3903
Size: 274 80 33 51 1443 283 1334 309
Cuts at: 3903 5534 7246 96
Size: 1631 1712 138
Fragments arranged by size:
1712 1631 1443 1334 309 283 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2311 2343 2642 2674 4469 4501 7248
Size: 32 134 32 299 32 1795 32 2747
Cuts at: 7248 7280 2145
Size: 32 2153
Fragments arranged by size:
2747 2153 1795 299 134 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4017 1316
Size: 2701 4587
XbaI T'CTAG_A
Cuts at: 4624 4624
Size: 7288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2710 2710
Size: 7288
XhoI C'TCGA_G
Cuts at: 1943 2256 1943
Size: 313 6975
XmaI C'CCGG_G
Cuts at: 1870 4613 1870
Size: 2743 4545
XmnI GAAnn'nnTTC
Cuts at: 2495 3151 5417 2495
Size: 656 2266 4366
Fragments arranged by size:
4366 2266 656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5096 276
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI