Restriction map for
pFN21AB8491
(Circular) (Six-base) MAPSORT of: pFN21AB8491.seq Check: 1284 from: 1 to: 7414
pFN21AB8491 7414 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3541 3541
Size: 7414
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5224 278
Size: 53 83 186 936 3688 2468
Fragments arranged by size:
3688 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4191 4757 1937
Size: 2254 566 4594
Fragments arranged by size:
4594 2254 566
Acc65I G'GTAC_C
Cuts at: 4735 4735
Size: 7414
AclI AA'CG_TT
Cuts at: 1563 5541 5914 1563
Size: 3978 373 3063
Fragments arranged by size:
3978 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 3851 4517 5477 6861 7129 1632
Size: 489 1730 666 960 1384 268 1917
Fragments arranged by size:
1917 1730 1384 960 666 489 268
AflII C'TTAA_G
Cuts at: 820 1017 3110 820
Size: 197 2093 5124
Fragments arranged by size:
5124 2093 197
AflIII A'CryG_T
Cuts at: 1203 6313 7290 1203
Size: 5110 977 1327
Fragments arranged by size:
5110 1327 977
AgeI A'CCGG_T
Cuts at: 6245 6245
Size: 7414
AhdI GACnn_n'nnGTC
Cuts at: 6143 6143
Size: 7414
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2913 1184
Size: 1729 5685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 7382
AlwNI CAG_nnn'CTG
Cuts at: 2560 2623 3130 3466 3490 3628 3964 4153 5157
Size: 63 507 336 24 138 336 189 1004
Cuts at: 5157 6729 2560
Size: 1572 3245
Fragments arranged by size:
3245 1572 1004 507 336 336 189 138
63 24
ApaI G_GGCC'C
Cuts at: 3094 3932 3094
Size: 838 6576
ApaLI G'TGCA_C
Cuts at: 5471 6627 5471
Size: 1156 6258
ApoI r'AATT_y
Cuts at: 1493 4135 4723 4949 5101 7210 1493
Size: 2642 588 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5968 160
Size: 872 4936 1606
Fragments arranged by size:
4936 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2160 2631 3015 3582 3764 4452
Size: 73 96 121 471 384 567 182 688
Cuts at: 4452 4678 4739 1870
Size: 226 61 4545
Fragments arranged by size:
4545 688 567 471 384 226 182 121
96 73 61
BamHI G'GATC_C
Cuts at: 2362 3336 4744 5111 2362
Size: 974 1408 367 4665
Fragments arranged by size:
4665 1408 974 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4668 4735 6190 618
Size: 325 217 627 2881 67 1455 1842
Fragments arranged by size:
2881 1842 1455 627 325 217 67
BanII G_rGCy'C
Cuts at: 1391 2085 2167 2811 3094 3573 3932 3972 4468
Size: 694 82 644 283 479 359 40 496
Cuts at: 4468 4733 1391
Size: 265 4072
Fragments arranged by size:
4072 694 644 496 479 359 283 265
82 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6443
BbvCI CC'TCA_GC
Cuts at: 2135 3558 4476 2135
Size: 1423 918 5073
Fragments arranged by size:
5073 1423 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5605 5639 1903
Size: 34 3668 34 3678
Fragments arranged by size:
3678 3668 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3256 5306 6516 3256
Size: 2050 1210 4154
Fragments arranged by size:
4154 2050 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7414
BfrBI ATG'CAT
Cuts at: 2570 2570
Size: 7414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2873 6025 136
Size: 107 122 71 631 605 1201 3152 1525
Fragments arranged by size:
3152 1525 1201 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3910 7409 3910
Size: 3499 3915
BlpI GC'TnA_GC
Cuts at: 2476 2757 3707 4116 4831 2476
Size: 281 950 409 715 5059
Fragments arranged by size:
5059 950 715 409 281
Bme1580I G_kGCm'C
Cuts at: 1790 3094 3932 4167 4671 5475 6631 1790
Size: 1304 838 235 504 804 1156 2573
Fragments arranged by size:
2573 1304 1156 838 804 504 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2022 3694 5145
Size: 416 53 83 399 24 605 1672 1451
Cuts at: 5145 6103 442
Size: 958 1753
Fragments arranged by size:
1753 1672 1451 958 605 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3506 1056
Size: 2450 4964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3165 4351 4612 3165
Size: 1186 261 5967
Fragments arranged by size:
5967 1186 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2510 2951 3365 3389 3650 3742 3897
Size: 1067 204 441 414 24 261 92 155
Cuts at: 3897 3986 4142 4175 4729 6074 1239
Size: 89 156 33 554 1345 2579
Fragments arranged by size:
2579 1345 1067 554 441 414 261 204
156 155 92 89 33 24
Bpu10I CC'TnA_GC
Cuts at: 2135 3402 3558 4294 4326 4476 2135
Size: 1267 156 736 32 150 5073
Fragments arranged by size:
5073 1267 736 156 150 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3720 4115 4233 5539 6404 6702 3720
Size: 395 118 1306 865 298 4432
Fragments arranged by size:
4432 1306 865 395 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3197 6077 882
Size: 617 1698 2880 2219
Fragments arranged by size:
2880 2219 1698 617
BsaAI yAC'GTr
Cuts at: 493 1204 2345 4259 5205 493
Size: 711 1141 1914 946 2702
Fragments arranged by size:
2702 1914 1141 946 711
BsaBI GATnn'nnATC
Cuts at: 7408 7408
Size: 7414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5221 5603 6238 275
Size: 53 83 186 936 3688 382 635 1451
Fragments arranged by size:
3688 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5847 6245 6519 6666 7077 5847
Size: 398 274 147 411 6184
Fragments arranged by size:
6184 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 3796 3826 4544
Size: 30 165 30 1080 30 1283 30 718
Cuts at: 4544 4574 1178
Size: 30 4018
Fragments arranged by size:
4018 1283 1080 718 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 2948 3152 3647 3732 3803 3808 3895
Size: 1321 441 204 495 85 71 5 87
Cuts at: 3895 4327 4705 1186
Size: 432 378 3895
Fragments arranged by size:
3895 1321 495 441 432 378 204 87
85 71 5
BseYI C'CCAG_C
Cuts at: 2112 2166 3020 3093 3207 3998 4361 4458 6617
Size: 54 854 73 114 791 363 97 2159
Cuts at: 6617 7113 2112
Size: 496 2413
Fragments arranged by size:
2413 2159 854 791 496 363 114 97
73 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3248 3332 3383 3996 1163
Size: 573 1512 84 51 613 4581
Fragments arranged by size:
4581 1512 613 573 84 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 4864 5625 6653 1540
Size: 275 186 679 2184 761 1028 2301
Fragments arranged by size:
2301 2184 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 3105 3573 5475 5560 6631 2811
Size: 294 468 1902 85 1071 3594
Fragments arranged by size:
3594 1902 1071 468 294 85
BsmI GAATG_Cn'
Cuts at: 3949 4925 5018 3949
Size: 976 93 6345
Fragments arranged by size:
6345 976 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2386 2386
Size: 7414
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2167 2811 3094 3105 3573 3932
Size: 399 295 82 644 283 11 468 359
Cuts at: 3932 3972 4167 4468 4671 4733 5475 5560 6631
Size: 40 195 301 203 62 742 85 1071
Cuts at: 6631 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 644 468 399 359 301
295 283 203 195 85 82 62 40
11
BspEI T'CCGG_A
Cuts at: 7077 7077
Size: 7414
BspHI T'CATG_A
Cuts at: 2197 5303 7033 2197
Size: 3106 1730 2578
Fragments arranged by size:
3106 2578 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3541 4769 844
Size: 2697 1228 3489
Fragments arranged by size:
3489 2697 1228
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 3004 3388 5301 1394
Size: 429 938 243 384 1913 3507
Fragments arranged by size:
3507 1913 938 429 384 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 5909 6083 65
Size: 1183 4661 174 1396
Fragments arranged by size:
4661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2745 6058 6245 1700
Size: 110 329 606 3313 187 2869
Fragments arranged by size:
3313 2869 606 329 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7414
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7414
BssSI C'ACGA_G
Cuts at: 5474 6486 5474
Size: 1012 6402
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3670 4105 4150 794
Size: 2876 435 45 4058
Fragments arranged by size:
4058 2876 435 45
BstBI TT'CG_AA
Cuts at: 6218 6218
Size: 7414
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7414
BstXI CCAn_nnnn'nTGG
Cuts at: 3823 3823
Size: 7414
BstYI r'GATC_y
Cuts at: 1972 2050 2362 3336 3910 4066 4480 4575 4744
Size: 78 312 974 574 156 414 95 169
Cuts at: 4744 5111 5496 5513 6965 7051 7063 7409 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 974 574 414 385 367 346
312 169 156 95 86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3434 3771 4059 3434
Size: 337 288 6789
Fragments arranged by size:
6789 337 288
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2661 2868 3816 3822 4299
Size: 552 939 150 507 207 948 6 477
Cuts at: 4299 513
Size: 3628
Fragments arranged by size:
3628 948 939 552 507 477 207 150
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6675
Fragments arranged by size:
6675 683 56
BtsI GCAGTG_nn'
Cuts at: 4360 4372 4930 5724 5744 4360
Size: 12 558 794 20 6030
Fragments arranged by size:
6030 794 558 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7379
DraI TTT'AAA
Cuts at: 4718 4718
Size: 7414
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6421 809
Size: 507 5105 1802
Fragments arranged by size:
5105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2522 3492 3819
Size: 54 1425 50 275 18 692 970 327
Cuts at: 3819 4525 4861 5750 7379 8
Size: 706 336 889 1629 43
Fragments arranged by size:
1629 1425 970 889 706 692 336 327
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4861 1537
Size: 275 3049 4090
Fragments arranged by size:
4090 3049 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2994 3060 3167 4493 5344 1353
Size: 1041 600 66 107 1326 851 3423
Fragments arranged by size:
3423 1326 1041 851 600 107 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 2162 6001 6375 6521 387
Size: 1064 138 471 102 3839 374 146 1280
Fragments arranged by size:
3839 1280 1064 471 374 146 138 102
EcoICRI GAG'CTC
Cuts at: 2809 3571 2809
Size: 762 6652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2306 2510 2951 3365 3389 3650
Size: 393 489 185 204 441 414 24 261
Cuts at: 3650 3742 3851 3897 3986 4142 4175 4517 4729
Size: 92 109 46 89 156 33 342 212
Cuts at: 4729 5477 6074 6861 7129 1239
Size: 748 597 787 268 1524
Fragments arranged by size:
1524 787 748 597 489 441 414 393
342 268 261 212 204 185 156 109
92 89 46 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 2861 3433 2199
Size: 662 572 6180
Fragments arranged by size:
6180 662 572
EcoO109I rG'GnC_Cy
Cuts at: 2528 2875 3463 3929 4002 4020 4087 4126 4321
Size: 347 588 466 73 18 67 39 195
Cuts at: 4321 2528
Size: 5621
Fragments arranged by size:
5621 588 466 347 195 73 67 39
18
EcoRI G'AATT_C
Cuts at: 4723 5101 4723
Size: 378 7036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 7414
FspI TGC'GCA
Cuts at: 5920 5920
Size: 7414
HaeII r_GCGC'y
Cuts at: 1678 2382 2611 3131 3884 6561 1678
Size: 704 229 520 753 2677 2531
Fragments arranged by size:
2677 2531 753 704 520 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3022 3054 3796 3828 6055
Size: 32 135 32 1450 32 742 32 2227
Cuts at: 6055 6087 6129 6161 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2227 1450 742 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4192 4758 5004 669
Size: 988 281 2254 566 246 3079
Fragments arranged by size:
3079 2254 988 566 281 246
HindIII A'AGCT_T
Cuts at: 748 2236 748
Size: 1488 5926
HpaI GTT'AAC
Cuts at: 5004 5004
Size: 7414
KpnI G_GTAC'C
Cuts at: 4739 4739
Size: 7414
MfeI C'AATT_G
Cuts at: 5013 5013
Size: 7414
MluI A'CGCG_T
Cuts at: 7290 7290
Size: 7414
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2227 2943 3027 3135 3330 6528 6712 1305
Size: 922 716 84 108 195 3198 184 2007
Fragments arranged by size:
3198 2007 922 716 195 184 108 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3494 10
Size: 54 1425 343 1662 3930
Fragments arranged by size:
3930 1662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3687 3821 5372 5731 5890 518
Size: 648 2521 134 1551 359 159 2042
Fragments arranged by size:
2521 2042 1551 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 2018 2170 2980 3295 3334 3343 3367 3376
Size: 412 152 810 315 39 9 24 9
Cuts at: 3376 3457 3628 3640 3703 3998 4120 4414 4515
Size: 81 171 12 63 295 122 294 101
Cuts at: 4515 4830 5507 6655 6900 1606
Size: 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 412 315 315 295
294 245 171 152 122 101 81 63
39 24 12 9 9
NaeI GCC'GGC
Cuts at: 2141 2141
Size: 7414
NcoI C'CATG_G
Cuts at: 513 1065 2004 2868 3816 4299 513
Size: 552 939 864 948 483 3628
Fragments arranged by size:
3628 948 939 864 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7414
NgoMIV G'CCGG_C
Cuts at: 2139 2139
Size: 7414
NheI G'CTAG_C
Cuts at: 1052 3502 1052
Size: 2450 4964
NotI GC'GGCC_GC
Cuts at: 4861 4861
Size: 7414
NsiI A_TGCA'T
Cuts at: 2572 2572
Size: 7414
NspI r_CATG'y
Cuts at: 1132 1229 4772 6317 1132
Size: 97 3543 1545 2229
Fragments arranged by size:
3543 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2078 1348
Size: 730 6684
PciI A'CATG_T
Cuts at: 6313 6313
Size: 7414
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 3628 3964 4153 1108
Size: 1380 1140 336 189 4369
Fragments arranged by size:
4369 1380 1140 336 189
PfoI T'CCnGG_A
Cuts at: 4069 4069
Size: 7414
PmeI GTTT'AAAC
Cuts at: 4718 4718
Size: 7414
PmlI CAC'GTG
Cuts at: 2345 2345
Size: 7414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 5483 5515 6996 7028 4544
Size: 32 907 32 1481 32 4930
Fragments arranged by size:
4930 1481 907 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3463 4321 3463
Size: 858 6556
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7414
PsiI TTA'TAA
Cuts at: 4984 4984
Size: 7414
PspOMI G'GGCC_C
Cuts at: 3090 3928 3090
Size: 838 6576
PstI C_TGCA'G
Cuts at: 830 2628 2910 2997 3135 3294 3462 3612 3666
Size: 1798 282 87 138 159 168 150 54
Cuts at: 3666 3678 4766 830
Size: 12 1088 3478
Fragments arranged by size:
3478 1798 1088 282 168 159 150 138
87 54 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7414
PvuII CAG'CTG
Cuts at: 2018 2170 3295 3343 3367 3457 3628 3703 3998
Size: 152 1125 48 24 90 171 75 295
Cuts at: 3998 4120 4414 2018
Size: 122 294 5018
Fragments arranged by size:
5018 1125 295 294 171 152 122 90
75 48 24
SacI G_AGCT'C
Cuts at: 2811 3573 2811
Size: 762 6652
SalI G'TCGA_C
Cuts at: 1936 4190 4756 1936
Size: 2254 566 4594
Fragments arranged by size:
4594 2254 566
SanDI GG'GwC_CC
Cuts at: 4321 4321
Size: 7414
SapI GCTCTTCn'nnn_
Cuts at: 2994 3167 2994
Size: 173 7241
SbfI CC_TGCA'GG
Cuts at: 2997 4766 2997
Size: 1769 5645
ScaI AGT'ACT
Cuts at: 5662 7374 5662
Size: 1712 5702
SfcI C'TryA_G
Cuts at: 826 1045 2624 2645 2906 2993 3131 3290 3458
Size: 219 1579 21 261 87 138 159 168
Cuts at: 3458 3608 3662 3674 4623 4762 5897 6578 6769
Size: 150 54 12 949 139 1135 681 191
Cuts at: 6769 826
Size: 1471
Fragments arranged by size:
1579 1471 1135 949 681 261 219 191
168 159 150 139 138 87 54 21
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2873 2873
Size: 7414
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7414
SmaI CCC'GGG
Cuts at: 1872 3584 3766 4741 1872
Size: 1712 182 975 4545
Fragments arranged by size:
4545 1712 975 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3110 3735 4130 4248 5518 6419
Size: 197 926 1167 625 395 118 1270 901
Cuts at: 6419 6681 820
Size: 262 1553
Fragments arranged by size:
1553 1270 1167 926 901 625 395 262
197 118
SnaBI TAC'GTA
Cuts at: 493 5205 493
Size: 4712 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7414
SphI G_CATG'C
Cuts at: 1132 4772 1132
Size: 3640 3774
SspI AAT'ATT
Cuts at: 5 52 5338 5
Size: 47 5286 2081
Fragments arranged by size:
5286 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7414
StyI C'CwwG_G
Cuts at: 513 1065 2004 2868 3816 4299 4406 4853 513
Size: 552 939 864 948 483 107 447 3074
Fragments arranged by size:
3074 948 939 864 552 483 447 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4559 5441 5458 5611 5796 6241 325
Size: 1255 2979 882 17 153 185 445 1498
Fragments arranged by size:
2979 1498 1255 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5660 7372 96
Size: 274 80 33 51 1443 3683 1712 138
Fragments arranged by size:
3683 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4257 4289 4648 4680 7374 7406 4257
Size: 32 359 32 2694 32 4265
Fragments arranged by size:
4265 2694 359 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7414
XbaI T'CTAG_A
Cuts at: 4750 4750
Size: 7414
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7414
XmaI C'CCGG_G
Cuts at: 1870 3582 3764 4739 1870
Size: 1712 182 975 4545
Fragments arranged by size:
4545 1712 975 182
XmnI GAAnn'nnTTC
Cuts at: 2307 5543 2307
Size: 3236 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5222 276
Size: 53 83 186 936 3688 2468
Fragments arranged by size:
3688 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NarI NruI PacI PspXI
PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI