Restriction map for
pFN21AB8486
(Circular) (Six-base) MAPSORT of: pFN21AB8486.seq Check: 5743 from: 1 to: 7270
pFN21AB8486 7270 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3200 4337 3200
Size: 1137 6133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5080 278
Size: 53 83 186 936 3544 2468
Fragments arranged by size:
3544 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4613 1937
Size: 2676 4594
Acc65I G'GTAC_C
Cuts at: 4591 4591
Size: 7270
AclI AA'CG_TT
Cuts at: 1563 5397 5770 1563
Size: 3834 373 3063
Fragments arranged by size:
3834 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2425 2588 3577 5333 6717 6985 1632
Size: 764 29 163 989 1756 1384 268 1917
Fragments arranged by size:
1917 1756 1384 989 764 268 163 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7073
AflIII A'CryG_T
Cuts at: 1203 6169 7146 1203
Size: 4966 977 1327
Fragments arranged by size:
4966 1327 977
AgeI A'CCGG_T
Cuts at: 6101 6101
Size: 7270
AhdI GACnn_n'nnGTC
Cuts at: 2800 5999 2800
Size: 3199 4071
AleI CACnn'nnGTG
Cuts at: 1166 2588 1166
Size: 1422 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7270
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 7238
AlwNI CAG_nnn'CTG
Cuts at: 2497 5013 6585 2497
Size: 2516 1572 3182
Fragments arranged by size:
3182 2516 1572
ApaI G_GGCC'C
Cuts at: 2023 2042 2411 2746 2023
Size: 19 369 335 6547
Fragments arranged by size:
6547 369 335 19
ApaLI G'TGCA_C
Cuts at: 5327 6483 5327
Size: 1156 6114
ApoI r'AATT_y
Cuts at: 1493 2302 3152 4579 4805 4957 7066 1493
Size: 809 850 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 850 809 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5824 160
Size: 872 4792 1606
Fragments arranged by size:
4792 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 3364 4595 1870
Size: 73 215 1206 1231 4545
Fragments arranged by size:
4545 1231 1206 215 73
AvrII C'CTAG_G
Cuts at: 3008 3008
Size: 7270
BamHI G'GATC_C
Cuts at: 4600 4967 4600
Size: 367 6903
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2888 3118 3188 4591 6046
Size: 325 217 627 1101 230 70 1403 1455
Cuts at: 6046 618
Size: 1842
Fragments arranged by size:
1842 1455 1403 1101 627 325 230 217
70
BanII G_rGCy'C
Cuts at: 1391 2023 2042 2411 2746 2855 2865 3174 3456
Size: 632 19 369 335 109 10 309 282
Cuts at: 3456 3738 4589 1391
Size: 282 851 4072
Fragments arranged by size:
4072 851 632 369 335 309 282 282
109 19 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 4210 928
Size: 971 364 1947 3988
Fragments arranged by size:
3988 1947 971 364
BbvCI CC'TCA_GC
Cuts at: 4385 4385
Size: 7270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2440 2474 5461 5495 1903
Size: 34 503 34 2987 34 3678
Fragments arranged by size:
3678 2987 503 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 5162 6372 3407
Size: 1755 1210 4305
Fragments arranged by size:
4305 1755 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7270
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4232 5881 136
Size: 107 122 71 631 605 2560 1649 1525
Fragments arranged by size:
2560 1649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7265 7265
Size: 7270
BlpI GC'TnA_GC
Cuts at: 2771 4687 2771
Size: 1916 5354
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2042 2411 2746 3123 3191 3860 5331
Size: 233 19 369 335 377 68 669 1471
Cuts at: 5331 6487 1790
Size: 1156 2573
Fragments arranged by size:
2573 1471 1156 669 377 369 335 233
68 19
BmgBI CAC'GTC
Cuts at: 1318 2254 1318
Size: 936 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 4258 4572
Size: 416 53 83 399 24 1824 1017 314
Cuts at: 4572 5001 5959 442
Size: 429 958 1753
Fragments arranged by size:
1824 1753 1017 958 429 416 399 314
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3858 4192 3858
Size: 334 6936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3188 3334 4169 4175 5930 1239
Size: 1949 146 835 6 1755 2579
Fragments arranged by size:
2579 1949 1755 835 146 6
Bpu10I CC'TnA_GC
Cuts at: 2849 4385 2849
Size: 1536 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 3763 4070 5395 6260 6558 2048
Size: 1715 307 1325 865 298 2760
Fragments arranged by size:
2760 1715 1325 865 307 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3964 5933 882
Size: 617 2465 1969 2219
Fragments arranged by size:
2465 2219 1969 617
BsaAI yAC'GTr
Cuts at: 493 1204 3038 3208 3378 3489 5061 493
Size: 711 1834 170 170 111 1572 2702
Fragments arranged by size:
2702 1834 1572 711 170 170 111
BsaBI GATnn'nnATC
Cuts at: 7264 7264
Size: 7270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 3471 4206 5077
Size: 53 83 186 936 1224 714 735 871
Cuts at: 5077 5459 6094 275
Size: 382 635 1451
Fragments arranged by size:
1451 1224 936 871 735 714 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3399 5703 6101 6375 6522 6933 3399
Size: 2304 398 274 147 411 3736
Fragments arranged by size:
3736 2304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2315 2339 2345 2881
Size: 30 165 30 906 6 24 6 536
Cuts at: 2881 2885 2911 2915 3659 3689 1178
Size: 4 26 4 744 30 4759
Fragments arranged by size:
4759 906 744 536 165 30 30 30
26 24 6 6 4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2519 2543 2914 1186
Size: 1003 330 24 371 5542
Fragments arranged by size:
5542 1003 371 330 24
BseYI C'CCAG_C
Cuts at: 2166 2212 3423 4251 4479 6473 6969 2166
Size: 46 1211 828 228 1994 496 2467
Fragments arranged by size:
2467 1994 1211 828 496 228 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 2897 3085 3418 3592 4091 1163
Size: 573 906 255 188 333 174 499 4342
Fragments arranged by size:
4342 906 573 499 333 255 188 174
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 4720 5481 6509 1540
Size: 275 186 16 2703 761 1028 2301
Fragments arranged by size:
2703 2301 1028 761 275 186 16
BsiHKAI G_wGCw'C
Cuts at: 3456 5331 5416 6487 3456
Size: 1875 85 1071 4239
Fragments arranged by size:
4239 1875 1071 85
BsmI GAATG_Cn'
Cuts at: 4781 4874 4781
Size: 93 7177
BsmBI CGTCTCn'nnnn_
Cuts at: 4479 4479
Size: 7270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2042 2411 2746 2855 2865 3123
Size: 399 233 19 369 335 109 10 258
Cuts at: 3123 3174 3191 3456 3738 3860 4589 5331 5416
Size: 51 17 265 282 122 729 742 85
Cuts at: 5416 6487 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 729 399 369 335 282
265 258 233 122 109 85 51 19
17 10
BspEI T'CCGG_A
Cuts at: 3399 6933 3399
Size: 3534 3736
BspHI T'CATG_A
Cuts at: 5159 6889 5159
Size: 1730 5540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3171 3200 3880 4337 4625 844
Size: 2327 29 680 457 288 3489
Fragments arranged by size:
3489 2327 680 457 288 29
BsrBI CCG'CTC
Cuts at: 1394 1823 2723 2968 5157 1394
Size: 429 900 245 2189 3507
Fragments arranged by size:
3507 2189 900 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 3643 5765 5939 65
Size: 1183 2395 2122 174 1396
Fragments arranged by size:
2395 2122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3317 3473 4162 4226 4466 5914 6101
Size: 110 1507 156 689 64 240 1448 187
Cuts at: 6101 1700
Size: 2869
Fragments arranged by size:
2869 1507 1448 689 240 187 156 110
64
BsrGI T'GTAC_A
Cuts at: 96 2385 2838 4518 96
Size: 2289 453 1680 2848
Fragments arranged by size:
2848 2289 1680 453
BssHII G'CGCG_C
Cuts at: 1925 2558 4281 1925
Size: 633 1723 4914
Fragments arranged by size:
4914 1723 633
BssSI C'ACGA_G
Cuts at: 2081 3832 3843 5330 6342 2081
Size: 1751 11 1487 1012 3009
Fragments arranged by size:
3009 1751 1487 1012 11
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4371 794
Size: 3577 3693
BstBI TT'CG_AA
Cuts at: 6074 6074
Size: 7270
BstEII G'GTnAC_C
Cuts at: 1184 2581 1184
Size: 1397 5873
BstXI CCAn_nnnn'nTGG
Cuts at: 3955 3955
Size: 7270
BstYI r'GATC_y
Cuts at: 1972 4305 4455 4600 4967 5352 5369 6821 6907
Size: 2333 150 145 367 385 17 1452 86
Cuts at: 6907 6919 7265 1972
Size: 12 346 1977
Fragments arranged by size:
2333 1977 1452 385 367 346 150 145
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2552 2604 2665 2856 4179 513
Size: 552 939 548 52 61 191 1323 3604
Fragments arranged by size:
3604 1323 939 552 548 191 61 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 3462 4123 487
Size: 683 56 1217 1019 661 3634
Fragments arranged by size:
3634 1217 1019 683 661 56
BtsI GCAGTG_nn'
Cuts at: 3638 3970 4022 4786 5580 5600 3638
Size: 332 52 764 794 20 5308
Fragments arranged by size:
5308 794 764 332 52 20
ClaI AT'CG_AT
Cuts at: 1554 3981 1554
Size: 2427 4843
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2323 2358 529
Size: 35 1759 35 5441
Fragments arranged by size:
5441 1759 35 35
DraI TTT'AAA
Cuts at: 4574 4574
Size: 7270
DraIII CAC_nnn'GTG
Cuts at: 2500 4017 2500
Size: 1517 5753
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 6277 809
Size: 507 942 4019 1802
Fragments arranged by size:
4019 1802 942 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2523 3475
Size: 54 1425 50 275 18 522 171 952
Cuts at: 3475 4717 5606 7235 8
Size: 1242 889 1629 43
Fragments arranged by size:
1629 1425 1242 952 889 522 275 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4717 1537
Size: 275 2905 4090
Fragments arranged by size:
4090 2905 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3521 5200 1353
Size: 2168 1679 3423
Fragments arranged by size:
3423 2168 1679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 4460 4468 5857 6231 6377
Size: 1064 138 457 2414 8 1389 374 146
Cuts at: 6377 387
Size: 1280
Fragments arranged by size:
2414 1389 1280 1064 457 374 146 138
8
EcoICRI GAG'CTC
Cuts at: 3454 3454
Size: 7270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2425 2588 3188 3334 3577 4169
Size: 393 764 29 163 600 146 243 592
Cuts at: 4169 4175 5333 5930 6717 6985 1239
Size: 6 1158 597 787 268 1524
Fragments arranged by size:
1524 1158 787 764 600 597 592 393
268 243 163 146 29 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 4295 2501
Size: 1794 5476
EcoO109I rG'GnC_Cy
Cuts at: 2038 2408 2628 2743 3243 4424 2038
Size: 370 220 115 500 1181 4884
Fragments arranged by size:
4884 1181 500 370 220 115
EcoRI G'AATT_C
Cuts at: 4579 4957 4579
Size: 378 6892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3532 3163
Size: 369 6901
FspI TGC'GCA
Cuts at: 5776 5776
Size: 7270
HaeII r_GCGC'y
Cuts at: 1678 2167 4062 6417 1678
Size: 489 1895 2355 2531
Fragments arranged by size:
2531 2355 1895 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 2786 2818 3659
Size: 32 135 32 1175 32 7 32 841
Cuts at: 3659 3691 5911 5943 5985 6017 1373
Size: 32 2220 32 42 32 2626
Fragments arranged by size:
2626 2220 1175 841 135 42 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2128 4614 4860 669
Size: 988 281 190 2486 246 3079
Fragments arranged by size:
3079 2486 988 281 246 190
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7270
HpaI GTT'AAC
Cuts at: 4860 4860
Size: 7270
KpnI G_GTAC'C
Cuts at: 4595 4595
Size: 7270
MfeI C'AATT_G
Cuts at: 4869 4869
Size: 7270
MluI A'CGCG_T
Cuts at: 7146 7146
Size: 7270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2352 3690 6384 6568 1305
Size: 1047 1338 2694 184 2007
Fragments arranged by size:
2694 2007 1338 1047 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 10
Size: 54 1425 343 522 4926
Fragments arranged by size:
4926 1425 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2588 3116 3447 5228 5587 5746 518
Size: 648 1422 528 331 1781 359 159 2042
Fragments arranged by size:
2042 1781 1422 648 528 359 331 159
MspA1I CmG'CkG
Cuts at: 1606 2391 2554 2643 2667 2733 3004 3021 3723
Size: 785 163 89 24 66 271 17 702
Cuts at: 3723 4389 4686 5363 6511 6756 1606
Size: 666 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 785 702 677 666 297 271
245 163 89 66 24 17
NaeI GCC'GGC
Cuts at: 3475 4228 3475
Size: 753 6517
NcoI C'CATG_G
Cuts at: 513 1065 2004 2856 4179 513
Size: 552 939 852 1323 3604
Fragments arranged by size:
3604 1323 939 852 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7270
NgoMIV G'CCGG_C
Cuts at: 3473 4226 3473
Size: 753 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7270
NotI GC'GGCC_GC
Cuts at: 4717 4717
Size: 7270
NspI r_CATG'y
Cuts at: 1132 1229 3137 3905 4628 6173 1132
Size: 97 1908 768 723 1545 2229
Fragments arranged by size:
2229 1908 1545 768 723 97
PasI CC'CwG_GG
Cuts at: 1348 3046 4064 1348
Size: 1698 1018 4554
Fragments arranged by size:
4554 1698 1018
PciI A'CATG_T
Cuts at: 6169 6169
Size: 7270
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2872 4017 1108
Size: 1764 1145 4361
Fragments arranged by size:
4361 1764 1145
PmeI GTTT'AAAC
Cuts at: 4574 4574
Size: 7270
PmlI CAC'GTG
Cuts at: 3378 3489 3378
Size: 111 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 5339 5371 6852 6884 2315
Size: 32 2992 32 1481 32 2701
Fragments arranged by size:
2992 2701 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2628 3243 4424 2628
Size: 615 1181 5474
Fragments arranged by size:
5474 1181 615
PshAI GACnn'nnGTC
Cuts at: 1613 4429 1613
Size: 2816 4454
PsiI TTA'TAA
Cuts at: 4840 4840
Size: 7270
PspOMI G'GGCC_C
Cuts at: 2019 2038 2407 2742 2019
Size: 19 369 335 6547
Fragments arranged by size:
6547 369 335 19
PstI C_TGCA'G
Cuts at: 830 2648 3464 4622 830
Size: 1818 816 1158 3478
Fragments arranged by size:
3478 1818 1158 816
PvuI CG_AT'CG
Cuts at: 2001 2017 2001
Size: 16 7254
PvuII CAG'CTG
Cuts at: 2643 3004 3021 2643
Size: 361 17 6892
Fragments arranged by size:
6892 361 17
SacI G_AGCT'C
Cuts at: 3456 3456
Size: 7270
SacII CC_GC'GG
Cuts at: 2555 2668 2555
Size: 113 7157
SalI G'TCGA_C
Cuts at: 1936 4612 1936
Size: 2676 4594
SanDI GG'GwC_CC
Cuts at: 4424 4424
Size: 7270
SbfI CC_TGCA'GG
Cuts at: 4622 4622
Size: 7270
ScaI AGT'ACT
Cuts at: 5518 7230 5518
Size: 1712 5558
SexAI A'CCwGG_T
Cuts at: 2496 2826 2496
Size: 330 6940
SfcI C'TryA_G
Cuts at: 826 1045 2644 3460 3781 4618 5753 6434 6625
Size: 219 1599 816 321 837 1135 681 191
Cuts at: 6625 826
Size: 1471
Fragments arranged by size:
1599 1471 1135 837 816 681 321 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7270
SmaI CCC'GGG
Cuts at: 1872 2160 3366 4597 1872
Size: 288 1206 1231 4545
Fragments arranged by size:
4545 1231 1206 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 3742 4085 5374 6275 6537
Size: 197 926 120 1679 343 1289 901 262
Cuts at: 6537 820
Size: 1553
Fragments arranged by size:
1679 1553 1289 926 901 343 262 197
120
SnaBI TAC'GTA
Cuts at: 493 3038 5061 493
Size: 2545 2023 2702
Fragments arranged by size:
2702 2545 2023
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7270
SphI G_CATG'C
Cuts at: 1132 3905 4628 1132
Size: 2773 723 3774
Fragments arranged by size:
3774 2773 723
SspI AAT'ATT
Cuts at: 5 52 5194 5
Size: 47 5142 2081
Fragments arranged by size:
5142 2081 47
StuI AGG'CCT
Cuts at: 1517 3674 1517
Size: 2157 5113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2856 3008 3198 3684 4179 4709
Size: 552 939 852 152 190 486 495 530
Cuts at: 4709 513
Size: 3074
Fragments arranged by size:
3074 939 852 552 530 495 486 190
152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 3103 4277 5297 5314 5467 5652
Size: 1255 578 945 1174 1020 17 153 185
Cuts at: 5652 6097 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1174 1020 945 578 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2385 2620
Size: 274 80 33 51 1443 90 318 235
Cuts at: 2620 2838 4170 4518 5516 7228 96
Size: 218 1332 348 998 1712 138
Fragments arranged by size:
1712 1443 1332 998 348 318 274 235
218 138 90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2881 2913 7230 7262 2881
Size: 32 4317 32 2889
Fragments arranged by size:
4317 2889 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7270
XbaI T'CTAG_A
Cuts at: 4606 4606
Size: 7270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3205 3635 3205
Size: 430 6840
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7270
XmaI C'CCGG_G
Cuts at: 1870 2158 3364 4595 1870
Size: 288 1206 1231 4545
Fragments arranged by size:
4545 1231 1206 288
XmnI GAAnn'nnTTC
Cuts at: 2048 2270 3500 5399 2048
Size: 222 1230 1899 3919
Fragments arranged by size:
3919 1899 1230 222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5078 276
Size: 53 83 186 936 3544 2468
Fragments arranged by size:
3544 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspXI PsrI RsrII SapI SfiI SfoI SrfI
SwaI