Restriction map for
pFN21AB8481
(Circular) (Six-base) MAPSORT of: pFN21AB8481.seq Check: 6517 from: 1 to: 7453
pFN21AB8481 7453 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5263 278
Size: 53 83 186 936 3727 2468
Fragments arranged by size:
3727 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3275 4691 4796 1937
Size: 1338 1416 105 4594
Fragments arranged by size:
4594 1416 1338 105
Acc65I G'GTAC_C
Cuts at: 4774 4774
Size: 7453
AclI AA'CG_TT
Cuts at: 1563 5580 5953 1563
Size: 4017 373 3063
Fragments arranged by size:
4017 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 2218 2810 2834 3528 3579 3744 3750
Size: 427 159 592 24 694 51 165 6
Cuts at: 3750 5516 6900 7168 1632
Size: 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 694 592 427 268 165
159 51 24 6
AfeI AGC'GCT
Cuts at: 2567 2567
Size: 7453
AflII C'TTAA_G
Cuts at: 820 1017 3341 820
Size: 197 2324 4932
Fragments arranged by size:
4932 2324 197
AflIII A'CryG_T
Cuts at: 1203 2013 2448 6352 7329 1203
Size: 810 435 3904 977 1327
Fragments arranged by size:
3904 1327 977 810 435
AgeI A'CCGG_T
Cuts at: 6284 6284
Size: 7453
AhdI GACnn_n'nnGTC
Cuts at: 6182 6182
Size: 7453
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7453
AlwNI CAG_nnn'CTG
Cuts at: 2326 2410 2683 3100 3722 5196 6768 2326
Size: 84 273 417 622 1474 1572 3011
Fragments arranged by size:
3011 1572 1474 622 417 273 84
ApaLI G'TGCA_C
Cuts at: 2360 4268 5510 6666 2360
Size: 1908 1242 1156 3147
Fragments arranged by size:
3147 1908 1242 1156
ApoI r'AATT_y
Cuts at: 1493 3356 4762 4988 5140 7249 1493
Size: 1863 1406 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6007 160
Size: 872 4975 1606
Fragments arranged by size:
4975 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 4778 1870
Size: 73 125 2710 4545
Fragments arranged by size:
4545 2710 125 73
BamHI G'GATC_C
Cuts at: 3659 4385 4783 5150 3659
Size: 726 398 367 5962
Fragments arranged by size:
5962 726 398 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2967 3011 4376 4774 6229
Size: 325 217 627 1180 44 1365 398 1455
Cuts at: 6229 618
Size: 1842
Fragments arranged by size:
1842 1455 1365 1180 627 398 325 217
44
BanII G_rGCy'C
Cuts at: 1391 4517 4772 1391
Size: 3126 255 4072
Fragments arranged by size:
4072 3126 255
BbeI G_GCGC'C
Cuts at: 2971 2971
Size: 7453
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 2798 4404 928
Size: 971 651 248 1606 3977
Fragments arranged by size:
3977 1606 971 651 248
BbvCI CC'TCA_GC
Cuts at: 3071 3071
Size: 7453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2155 2189 2321 2355 2614 2648 5644
Size: 34 218 34 132 34 259 34 2996
Cuts at: 5644 5678 1903
Size: 34 3678
Fragments arranged by size:
3678 2996 259 218 132 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5345 6555 5345
Size: 1210 6243
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6064 136
Size: 107 122 71 631 605 4392 1525
Fragments arranged by size:
4392 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7448 7448
Size: 7453
BlpI GC'TnA_GC
Cuts at: 2207 3038 4870 2207
Size: 831 1832 4790
Fragments arranged by size:
4790 1832 831
Bme1580I G_kGCm'C
Cuts at: 1790 2364 3122 3897 3936 4272 5514 6670 1790
Size: 574 758 775 39 336 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 775 758 574 336 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3273 5184 6142
Size: 416 53 83 399 24 1856 1911 958
Cuts at: 6142 442
Size: 1753
Fragments arranged by size:
1911 1856 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 7453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2507 2558 3047 3239 3522 6113 1239
Size: 1151 117 51 489 192 283 2591 2579
Fragments arranged by size:
2591 2579 1151 489 283 192 117 51
Bpu10I CC'TnA_GC
Cuts at: 2511 3071 2511
Size: 560 6893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 4531 5578 6443 6741 2543
Size: 1988 1047 865 298 3255
Fragments arranged by size:
3255 1988 1047 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4277 4442 4661 6116 882
Size: 617 555 2223 165 219 1455 2219
Fragments arranged by size:
2223 2219 1455 617 555 219 165
BsaAI yAC'GTr
Cuts at: 493 1204 5244 493
Size: 711 4040 2702
Fragments arranged by size:
4040 2702 711
BsaBI GATnn'nnATC
Cuts at: 7447 7447
Size: 7453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2642 2968 5260 5642
Size: 53 83 186 936 1109 326 2292 382
Cuts at: 5642 6277 275
Size: 635 1451
Fragments arranged by size:
2292 1451 1109 936 635 382 326 186
83 53
BsaWI w'CCGG_w
Cuts at: 2267 5886 6284 6558 6705 7116 2267
Size: 3619 398 274 147 411 2604
Fragments arranged by size:
3619 2604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2570 2600 1178
Size: 30 165 30 846 30 291 30 6031
Fragments arranged by size:
6031 846 291 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 2588 2591 2651 2735 2738 3065 3155
Size: 957 445 3 60 84 3 327 90
Cuts at: 3155 3380 3626 3815 4353 4370 4448 4493 4565
Size: 225 246 189 538 17 78 45 72
Cuts at: 4565 4691 1186
Size: 126 3948
Fragments arranged by size:
3948 957 538 445 327 246 225 189
126 90 84 78 72 60 45 17
3 3
BseYI C'CCAG_C
Cuts at: 2026 2138 2436 2440 2829 4516 6656 7152 2026
Size: 112 298 4 389 1687 2140 496 2327
Fragments arranged by size:
2327 2140 1687 496 389 298 112 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 2696 3537 1163
Size: 573 801 159 841 5079
Fragments arranged by size:
5079 841 801 573 159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2335 2913 4903 5664 6692 1540
Size: 275 186 334 578 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 578 334 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 4272 5514 5599 6670 2364
Size: 1908 1242 85 1071 3147
Fragments arranged by size:
3147 1908 1242 1071 85
BsiWI C'GTAC_G
Cuts at: 2451 2451
Size: 7453
BsmI GAATG_Cn'
Cuts at: 4964 5057 4964
Size: 93 7360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 3122 3897 3936 4272 4517 4772
Size: 399 574 758 775 39 336 245 255
Cuts at: 4772 5514 5599 6670 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 758 742 574 399 336
255 245 85 39
BspEI T'CCGG_A
Cuts at: 2267 7116 2267
Size: 4849 2604
BspHI T'CATG_A
Cuts at: 5342 7072 5342
Size: 1730 5723
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4808 844
Size: 3964 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2067 2112 2298 2826 2937 3031 5340
Size: 429 244 45 186 528 111 94 2309
Cuts at: 5340 1394
Size: 3507
Fragments arranged by size:
3507 2309 528 429 244 186 111 94
45
BsrDI GCAATG_nn'
Cuts at: 65 1248 5948 6122 65
Size: 1183 4700 174 1396
Fragments arranged by size:
4700 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2342 2553 2964 6097 6284 1700
Size: 110 236 296 211 411 3133 187 2869
Fragments arranged by size:
3133 2869 411 296 236 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7453
BssHII G'CGCG_C
Cuts at: 1925 2476 2616 1925
Size: 551 140 6762
Fragments arranged by size:
6762 551 140
BssSI C'ACGA_G
Cuts at: 2501 2717 5513 6525 2501
Size: 216 2796 1012 3429
Fragments arranged by size:
3429 2796 1012 216
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2683 794
Size: 1889 5564
BstBI TT'CG_AA
Cuts at: 6257 6257
Size: 7453
BstEII G'GTnAC_C
Cuts at: 1184 2053 4243 4564 1184
Size: 869 2190 321 4073
Fragments arranged by size:
4073 2190 869 321
BstYI r'GATC_y
Cuts at: 1972 2400 2463 2748 3659 4385 4783 5150 5535
Size: 428 63 285 911 726 398 367 385
Cuts at: 5535 5552 7004 7090 7102 7448 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 911 726 428 398 385 367
346 285 86 63 17 12
BstZ17I GTA'TAC
Cuts at: 3276 3276
Size: 7453
BtgI C'CryG_G
Cuts at: 513 1065 2128 513
Size: 552 1063 5838
Fragments arranged by size:
5838 1063 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 5198
Fragments arranged by size:
5198 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 4304 4471 4969 5763 5783 4304
Size: 167 498 794 20 5974
Fragments arranged by size:
5974 794 498 167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7418
DraI TTT'AAA
Cuts at: 4757 4757
Size: 7453
DraIII CAC_nnn'GTG
Cuts at: 3126 3126
Size: 7453
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4406 6460 809
Size: 507 3090 2054 1802
Fragments arranged by size:
3090 2054 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2607 2730
Size: 54 1425 50 275 18 369 408 123
Cuts at: 2730 2910 3444 4900 5789 7418 8
Size: 180 534 1456 889 1629 43
Fragments arranged by size:
1629 1456 1425 889 534 408 369 275
180 123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2910 4900 1537
Size: 275 1098 1990 4090
Fragments arranged by size:
4090 1990 1098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2865 2943 3222 3408 3456 3486 3564 3603
Size: 1512 78 279 186 48 30 78 39
Cuts at: 3603 3645 3867 3906 3945 3984 4023 4062 4143
Size: 42 222 39 39 39 39 39 81
Cuts at: 4143 4164 4287 4470 4650 4659 5383 1353
Size: 21 123 183 180 9 724 3423
Fragments arranged by size:
3423 1512 724 279 222 186 183 180
123 81 78 78 48 42 39 39
39 39 39 39 30 21 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 2203 2411 2929 6040 6414
Size: 1064 138 440 174 208 518 3111 374
Cuts at: 6414 6560 387
Size: 146 1280
Fragments arranged by size:
3111 1280 1064 518 440 374 208 174
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2218 2390 2507 2558 2810 2834
Size: 393 427 159 172 117 51 252 24
Cuts at: 2834 3047 3239 3522 3528 3579 3744 3750 5516
Size: 213 192 283 6 51 165 6 1766
Cuts at: 5516 6113 6900 7168 1239
Size: 597 787 268 1524
Fragments arranged by size:
1766 1524 787 597 427 393 283 268
252 213 192 172 165 159 117 51
51 24 6 6
EcoO109I rG'GnC_Cy
Cuts at: 3160 3418 3160
Size: 258 7195
EcoRI G'AATT_C
Cuts at: 4762 5140 4762
Size: 378 7075
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2584 4288 2584
Size: 1704 5749
FspI TGC'GCA
Cuts at: 2624 5959 2624
Size: 3335 4118
HaeII r_GCGC'y
Cuts at: 1678 2411 2569 2813 2971 3058 6600 1678
Size: 733 158 244 158 87 3542 2531
Fragments arranged by size:
3542 2531 733 244 158 158 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2984 3016 3276
Size: 32 135 32 1055 32 325 32 260
Cuts at: 3276 3308 3386 3418 3644 3676 4061 4093 6094
Size: 32 78 32 226 32 385 32 2001
Cuts at: 6094 6126 6168 6200 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2001 1055 385 325 260 226 135
78 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4797 5043 669
Size: 988 281 2859 246 3079
Fragments arranged by size:
3079 2859 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3338 748
Size: 2590 4863
HpaI GTT'AAC
Cuts at: 5043 5043
Size: 7453
KasI G'GCGC_C
Cuts at: 2967 2967
Size: 7453
KpnI G_GTAC'C
Cuts at: 4778 4778
Size: 7453
MfeI C'AATT_G
Cuts at: 5052 5052
Size: 7453
MluI A'CGCG_T
Cuts at: 2448 7329 2448
Size: 4881 2572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 2325 3373 6567 6751 1305
Size: 693 327 1048 3194 184 2007
Fragments arranged by size:
3194 2007 1048 693 327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5631
Fragments arranged by size:
5631 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 5411 5770 5929 518
Size: 648 1602 2643 359 159 2042
Fragments arranged by size:
2643 2042 1602 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2130 2317 2440 2521 2974 3100 4869
Size: 482 42 187 123 81 453 126 1769
Cuts at: 4869 5546 6694 6939 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 482 453 245 187
126 123 81 42
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 7453
NarI GG'CG_CC
Cuts at: 2968 2968
Size: 7453
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6901
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7453
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 7453
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7453
NotI GC'GGCC_GC
Cuts at: 4900 4900
Size: 7453
NspI r_CATG'y
Cuts at: 1132 1229 2173 4811 6356 1132
Size: 97 944 2638 1545 2229
Fragments arranged by size:
2638 2229 1545 944 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7453
PciI A'CATG_T
Cuts at: 6352 6352
Size: 7453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3100 1108
Size: 1992 5461
PfoI T'CCnGG_A
Cuts at: 3216 4388 3216
Size: 1172 6281
PmeI GTTT'AAAC
Cuts at: 4757 4757
Size: 7453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5522 5554 7035 7067 5522
Size: 32 1481 32 5908
Fragments arranged by size:
5908 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3160 3160
Size: 7453
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7453
PsiI TTA'TAA
Cuts at: 5023 5023
Size: 7453
PspXI vC'TCGA_Gb
Cuts at: 2068 2068
Size: 7453
PstI C_TGCA'G
Cuts at: 830 2322 2688 2979 4311 4805 830
Size: 1492 366 291 1332 494 3478
Fragments arranged by size:
3478 1492 1332 494 366 291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7453
PvuII CAG'CTG
Cuts at: 2317 2440 2521 2974 3100 2317
Size: 123 81 453 126 6670
Fragments arranged by size:
6670 453 126 123 81
SacII CC_GC'GG
Cuts at: 2131 2131
Size: 7453
SalI G'TCGA_C
Cuts at: 1936 4795 1936
Size: 2859 4594
SapI GCTCTTCn'nnn_
Cuts at: 4650 4650
Size: 7453
SbfI CC_TGCA'GG
Cuts at: 4805 4805
Size: 7453
ScaI AGT'ACT
Cuts at: 5701 7413 5701
Size: 1712 5741
SfcI C'TryA_G
Cuts at: 826 1045 2318 2684 2975 4307 4801 5936 6617
Size: 219 1273 366 291 1332 494 1135 681
Cuts at: 6617 6808 826
Size: 191 1471
Fragments arranged by size:
1471 1332 1273 1135 681 494 366 291
219 191
SfoI GGC'GCC
Cuts at: 2969 2969
Size: 7453
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7453
SmaI CCC'GGG
Cuts at: 1872 4780 1872
Size: 2908 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 2558 3341 4510 5557 6458
Size: 197 926 125 490 783 1169 1047 901
Cuts at: 6458 6720 820
Size: 262 1553
Fragments arranged by size:
1553 1169 1047 926 901 783 490 262
197 125
SnaBI TAC'GTA
Cuts at: 493 5244 493
Size: 4751 2702
SpeI A'CTAG_T
Cuts at: 152 4684 152
Size: 4532 2921
SphI G_CATG'C
Cuts at: 1132 2173 4811 1132
Size: 1041 2638 3774
Fragments arranged by size:
3774 2638 1041
SspI AAT'ATT
Cuts at: 5 52 5377 5
Size: 47 5325 2081
Fragments arranged by size:
5325 2081 47
StuI AGG'CCT
Cuts at: 1517 2426 1517
Size: 909 6544
StyI C'CwwG_G
Cuts at: 513 1065 2943 3015 3321 3795 3819 4143 4380
Size: 552 1878 72 306 474 24 324 237
Cuts at: 4380 4452 4892 513
Size: 72 440 3074
Fragments arranged by size:
3074 1878 552 474 440 324 306 237
72 72 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4757 5480 5497 5650 5835 6280 325
Size: 1255 3177 723 17 153 185 445 1498
Fragments arranged by size:
3177 1498 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5699 7411 96
Size: 274 80 33 51 1443 3722 1712 138
Fragments arranged by size:
3722 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7413 7445 7413
Size: 32 7421
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7453
XbaI T'CTAG_A
Cuts at: 4789 4789
Size: 7453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 3022 3097 3802 3904 3943 2488
Size: 534 75 705 102 39 5998
Fragments arranged by size:
5998 705 534 102 75 39
XhoI C'TCGA_G
Cuts at: 1943 2068 1943
Size: 125 7328
XmaI C'CCGG_G
Cuts at: 1870 4778 1870
Size: 2908 4545
XmnI GAAnn'nnTTC
Cuts at: 5582 5582
Size: 7453
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5261 276
Size: 53 83 186 936 3727 2468
Fragments arranged by size:
3727 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsmBI
BstXI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacI SanDI
SexAI SfiI SrfI SwaI