Restriction map for
pFN21AB8480
(Circular) (Six-base) MAPSORT of: pFN21AB8480.seq Check: 8019 from: 1 to: 7267
pFN21AB8480 7267 bp
With 182 enzymes: SgfI *
August 25, 2016 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5077 278
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3537 4563 4610 1937
Size: 1600 1026 47 4594
Fragments arranged by size:
4594 1600 1026 47
Acc65I G'GTAC_C
Cuts at: 2178 4588 2178
Size: 2410 4857
AclI AA'CG_TT
Cuts at: 1563 5394 5767 1563
Size: 3831 373 3063
Fragments arranged by size:
3831 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2284 3096 3989 4381 4530 5330 6714
Size: 519 133 812 893 392 149 800 1384
Cuts at: 6714 6982 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 893 812 800 519 392 268
149 133
AflII C'TTAA_G
Cuts at: 820 1017 4306 820
Size: 197 3289 3781
Fragments arranged by size:
3781 3289 197
AflIII A'CryG_T
Cuts at: 1203 6166 7143 1203
Size: 4963 977 1327
Fragments arranged by size:
4963 1327 977
AgeI A'CCGG_T
Cuts at: 6098 6098
Size: 7267
AhdI GACnn_n'nnGTC
Cuts at: 5996 5996
Size: 7267
AleI CACnn'nnGTG
Cuts at: 1166 2642 1166
Size: 1476 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2760 1184
Size: 1576 5691
AlwNI CAG_nnn'CTG
Cuts at: 2067 4325 5010 6582 2067
Size: 2258 685 1572 2752
Fragments arranged by size:
2752 2258 1572 685
ApaI G_GGCC'C
Cuts at: 2049 2049
Size: 7267
ApaLI G'TGCA_C
Cuts at: 5324 6480 5324
Size: 1156 6111
ApoI r'AATT_y
Cuts at: 1493 2521 3302 3377 3591 3613 3677 3974 4576
Size: 1028 781 75 214 22 64 297 602
Cuts at: 4576 4802 4954 7063 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 781 602 297 226 214
152 75 64 22
AseI AT'TA_AT
Cuts at: 160 1032 5821 160
Size: 872 4789 1606
Fragments arranged by size:
4789 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
AvaI C'yCGr_G
Cuts at: 1870 1943 4592 1870
Size: 73 2649 4545
Fragments arranged by size:
4545 2649 73
BamHI G'GATC_C
Cuts at: 2107 2225 4597 4964 2107
Size: 118 2372 367 4410
Fragments arranged by size:
4410 2372 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2164 2178 4588 6043 618
Size: 325 217 627 377 14 2410 1455 1842
Fragments arranged by size:
2410 1842 1455 627 377 325 217 14
BanII G_rGCy'C
Cuts at: 1391 2049 2514 3240 3388 3942 4586 1391
Size: 658 465 726 148 554 644 4072
Fragments arranged by size:
4072 726 658 644 554 465 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2952 3093 3135 3729 3893 3992 4380
Size: 971 1053 141 42 594 164 99 388
Cuts at: 4380 928
Size: 3815
Fragments arranged by size:
3815 1053 971 594 388 164 141 99
42
BbvCI CC'TCA_GC
Cuts at: 2863 2863
Size: 7267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5458 5492 1903
Size: 34 50 34 3437 34 3678
Fragments arranged by size:
3678 3437 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2641 5159 6369 2641
Size: 2518 1210 3539
Fragments arranged by size:
3539 2518 1210
BclI T'GATC_A
Cuts at: 1548 2466 2679 2820 4443 1548
Size: 918 213 141 1623 4372
Fragments arranged by size:
4372 1623 918 213 141
BfrBI ATG'CAT
Cuts at: 2534 2534
Size: 7267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 5878 136
Size: 107 122 71 631 605 766 3440 1525
Fragments arranged by size:
3440 1525 766 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2496 7262 2496
Size: 4766 2501
BlpI GC'TnA_GC
Cuts at: 4684 4684
Size: 7267
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2167 5328 6484 1790
Size: 259 118 3161 1156 2573
Fragments arranged by size:
3161 2573 1156 259 118
BmgBI CAC'GTC
Cuts at: 1318 2266 3407 1318
Size: 948 1141 5178
Fragments arranged by size:
5178 1141 948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2446 2565 3337
Size: 416 53 83 399 24 1029 119 772
Cuts at: 3337 4998 5956 442
Size: 1661 958 1753
Fragments arranged by size:
1753 1661 1029 958 772 416 399 119
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7267
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 7267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4292 5927 1239
Size: 3053 1635 2579
Fragments arranged by size:
3053 2579 1635
Bpu10I CC'TnA_GC
Cuts at: 2360 2863 2360
Size: 503 6764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3785 4203 5392 6257 6555 3785
Size: 418 1189 865 298 4497
Fragments arranged by size:
4497 1189 865 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3854 4295 5930 882
Size: 617 2355 441 1635 2219
Fragments arranged by size:
2355 2219 1635 617 441
BsaAI yAC'GTr
Cuts at: 493 1204 5058 493
Size: 711 3854 2702
Fragments arranged by size:
3854 2702 711
BsaBI GATnn'nnATC
Cuts at: 7261 7261
Size: 7267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5074 5456 6091 275
Size: 53 83 186 936 3541 382 635 1451
Fragments arranged by size:
3541 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2547 5700 6098 6372 6519 6930 2547
Size: 3153 398 274 147 411 2884
Fragments arranged by size:
3153 2884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7042
Fragments arranged by size:
7042 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2423 2426 2429 3305 3509 1186
Size: 942 295 3 3 876 204 4944
Fragments arranged by size:
4944 942 876 295 204 3 3
BseYI C'CCAG_C
Cuts at: 6470 6966 6470
Size: 496 6771
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 2766 3796 1163
Size: 573 601 429 1030 4634
Fragments arranged by size:
4634 1030 601 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 4717 5478 6506 1540
Size: 275 186 100 2616 761 1028 2301
Fragments arranged by size:
2616 2301 1028 761 275 186 100
BsiHKAI G_wGCw'C
Cuts at: 2514 3240 3412 5328 5413 6484 2514
Size: 726 172 1916 85 1071 3297
Fragments arranged by size:
3297 1916 1071 726 172 85
BsmI GAATG_Cn'
Cuts at: 2273 4778 4871 2273
Size: 2505 93 4669
Fragments arranged by size:
4669 2505 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2272 2417 2272
Size: 145 7122
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2167 2514 3240 3388 3412 3942
Size: 399 259 118 347 726 148 24 530
Cuts at: 3942 4586 5328 5413 6484 1391
Size: 644 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 726 644 530 399 347
259 148 118 85 24
BspEI T'CCGG_A
Cuts at: 6930 6930
Size: 7267
BspHI T'CATG_A
Cuts at: 4092 4209 5156 6886 4092
Size: 117 947 1730 4473
Fragments arranged by size:
4473 1730 947 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4622 844
Size: 3778 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5154 1394
Size: 429 3331 3507
Fragments arranged by size:
3507 3331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5762 5936 65
Size: 1183 4514 174 1396
Fragments arranged by size:
4514 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5911 6098 1700
Size: 110 4101 187 2869
Fragments arranged by size:
4101 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7267
BssSI C'ACGA_G
Cuts at: 5327 6339 5327
Size: 1012 6255
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 794
Size: 1653 5614
BstBI TT'CG_AA
Cuts at: 6071 6071
Size: 7267
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7267
BstYI r'GATC_y
Cuts at: 1972 2107 2225 2338 2496 2566 3547 4351 4408
Size: 135 118 113 158 70 981 804 57
Cuts at: 4408 4431 4597 4964 5349 5366 6818 6904 6916
Size: 23 166 367 385 17 1452 86 12
Cuts at: 6916 7262 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 804 385 367 346 166
158 135 118 113 86 70 57 23
17 12
Bsu36I CC'TnA_GG
Cuts at: 2376 2376
Size: 7267
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3078 487
Size: 683 56 1852 4676
Fragments arranged by size:
4676 1852 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 3351 4783 5577 5597 2283
Size: 1068 1432 794 20 3953
Fragments arranged by size:
3953 1432 1068 794 20
ClaI AT'CG_AT
Cuts at: 1554 3225 1554
Size: 1671 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7232
DraI TTT'AAA
Cuts at: 4571 4571
Size: 7267
DraIII CAC_nnn'GTG
Cuts at: 3407 3407
Size: 7267
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3994 6274 809
Size: 507 2678 2280 1802
Fragments arranged by size:
2678 2280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 4714 5603
Size: 54 1425 50 275 18 934 1950 889
Cuts at: 5603 7232 8
Size: 1629 43
Fragments arranged by size:
1950 1629 1425 934 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4714 1537
Size: 275 2902 4090
Fragments arranged by size:
4090 2902 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3108 3228 4032 4050 5197 1353
Size: 1755 120 804 18 1147 3423
Fragments arranged by size:
3423 1755 1147 804 120 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3668 5854 6228 6374 387
Size: 1064 138 2079 2186 374 146 1280
Fragments arranged by size:
2186 2079 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2512 3238 2512
Size: 726 6541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2284 3096 3989 4292 4381 4530
Size: 393 519 133 812 893 303 89 149
Cuts at: 4530 5330 5927 6714 6982 1239
Size: 800 597 787 268 1524
Fragments arranged by size:
1524 893 812 800 787 597 519 393
303 268 149 133 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 7267
EcoO109I rG'GnC_Cy
Cuts at: 2046 2046
Size: 7267
EcoRI G'AATT_C
Cuts at: 3302 3377 4576 4954 3302
Size: 75 1199 378 5615
Fragments arranged by size:
5615 1199 378 75
EcoRV GAT'ATC
Cuts at: 2752 3017 2752
Size: 265 7002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 7267
FspI TGC'GCA
Cuts at: 5773 5773
Size: 7267
HaeII r_GCGC'y
Cuts at: 1678 6414 1678
Size: 4736 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3107 3139 3253 3285 3462
Size: 32 135 32 1535 32 114 32 177
Cuts at: 3462 3494 3781 3813 4529 4561 5908 5940 5982
Size: 32 287 32 716 32 1347 32 42
Cuts at: 5982 6014 1373
Size: 32 2626
Fragments arranged by size:
2626 1535 1347 716 287 177 135 114
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 4611 4857 669
Size: 988 281 235 2438 246 3079
Fragments arranged by size:
3079 2438 988 281 246 235
HindIII A'AGCT_T
Cuts at: 748 2557 2730 3707 3743 748
Size: 1809 173 977 36 4272
Fragments arranged by size:
4272 1809 977 173 36
HpaI GTT'AAC
Cuts at: 2173 4857 2173
Size: 2684 4583
KpnI G_GTAC'C
Cuts at: 2182 4592 2182
Size: 2410 4857
MfeI C'AATT_G
Cuts at: 4866 4866
Size: 7267
MluI A'CGCG_T
Cuts at: 7143 7143
Size: 7267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 3354 3985 6381 6565 1305
Size: 1800 249 631 2396 184 2007
Fragments arranged by size:
2396 2007 1800 631 249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 10
Size: 54 1425 343 934 4511
Fragments arranged by size:
4511 1425 934 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2642 2835 3818 4156 5225 5584 5743
Size: 648 1476 193 983 338 1069 359 159
Cuts at: 5743 518
Size: 2042
Fragments arranged by size:
2042 1476 1069 983 648 359 338 193
159
MspA1I CmG'CkG
Cuts at: 1606 3967 4683 5360 6508 6753 1606
Size: 2361 716 677 1148 245 2120
Fragments arranged by size:
2361 2120 1148 716 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6715
NdeI CA'TA_TG
Cuts at: 387 4180 387
Size: 3793 3474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7267
NotI GC'GGCC_GC
Cuts at: 4714 4714
Size: 7267
NsiI A_TGCA'T
Cuts at: 2536 2536
Size: 7267
NspI r_CATG'y
Cuts at: 1132 1229 4424 4625 6170 1132
Size: 97 3195 201 1545 2229
Fragments arranged by size:
3195 2229 1545 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7267
PciI A'CATG_T
Cuts at: 6166 6166
Size: 7267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 3327 1108
Size: 1296 923 5048
Fragments arranged by size:
5048 1296 923
PmeI GTTT'AAAC
Cuts at: 4571 4571
Size: 7267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5336 5368 6849 6881 5336
Size: 32 1481 32 5722
Fragments arranged by size:
5722 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7267
PsiI TTA'TAA
Cuts at: 4837 4837
Size: 7267
PspOMI G'GGCC_C
Cuts at: 2045 2045
Size: 7267
PstI C_TGCA'G
Cuts at: 830 2346 4619 830
Size: 1516 2273 3478
Fragments arranged by size:
3478 2273 1516
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7267
PvuII CAG'CTG
Cuts at: 3967 3967
Size: 7267
SacI G_AGCT'C
Cuts at: 2514 3240 2514
Size: 726 6541
SalI G'TCGA_C
Cuts at: 1936 4609 1936
Size: 2673 4594
SapI GCTCTTCn'nnn_
Cuts at: 3228 3228
Size: 7267
SbfI CC_TGCA'GG
Cuts at: 4619 4619
Size: 7267
ScaI AGT'ACT
Cuts at: 2960 3953 5515 7227 2960
Size: 993 1562 1712 3000
Fragments arranged by size:
3000 1712 1562 993
SexAI A'CCwGG_T
Cuts at: 3870 3870
Size: 7267
SfcI C'TryA_G
Cuts at: 826 1045 2342 4435 4615 5750 6431 6622 826
Size: 219 1297 2093 180 1135 681 191 1471
Fragments arranged by size:
2093 1471 1297 1135 681 219 191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7267
SmaI CCC'GGG
Cuts at: 1872 4594 1872
Size: 2722 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3800 4218 4306 5371 6272 6534
Size: 197 926 1857 418 88 1065 901 262
Cuts at: 6534 820
Size: 1553
Fragments arranged by size:
1857 1553 1065 926 901 418 262 197
88
SnaBI TAC'GTA
Cuts at: 493 5058 493
Size: 4565 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7267
SphI G_CATG'C
Cuts at: 1132 4625 1132
Size: 3493 3774
SspI AAT'ATT
Cuts at: 5 52 5191 5
Size: 47 5139 2081
Fragments arranged by size:
5139 2081 47
StuI AGG'CCT
Cuts at: 1517 4014 1517
Size: 2497 4770
StyI C'CwwG_G
Cuts at: 513 1065 2311 2667 2689 3864 4706 513
Size: 552 1246 356 22 1175 842 3074
Fragments arranged by size:
3074 1246 1175 842 552 356 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5294 5311 5464 5649 6094 325
Size: 1255 3714 17 153 185 445 1498
Fragments arranged by size:
3714 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 3219 3951
Size: 274 80 33 51 1443 981 261 732
Cuts at: 3951 5513 7225 96
Size: 1562 1712 138
Fragments arranged by size:
1712 1562 1443 981 732 274 261 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7227 7259 7227
Size: 32 7235
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7267
XbaI T'CTAG_A
Cuts at: 4603 4603
Size: 7267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2774 4012 2722
Size: 52 1238 5977
Fragments arranged by size:
5977 1238 52
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7267
XmaI C'CCGG_G
Cuts at: 1870 4592 1870
Size: 2722 4545
XmnI GAAnn'nnTTC
Cuts at: 3900 5396 3900
Size: 1496 5771
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5075 276
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI