Restriction map for
pFN21AB8477
(Circular) (Six-base) MAPSORT of: pFN21AB8477.seq Check: 5266 from: 1 to: 7447
pFN21AB8477 7447 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2946 3941 2946
Size: 995 6452
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5257 278
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2178 2880 3481 4790 1937
Size: 241 702 601 1309 4594
Fragments arranged by size:
4594 1309 702 601 241
Acc65I G'GTAC_C
Cuts at: 4768 4768
Size: 7447
AclI AA'CG_TT
Cuts at: 1563 5574 5947 1563
Size: 4011 373 3063
Fragments arranged by size:
4011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2289 2571 2826 2870 3326 3537 3930
Size: 510 147 282 255 44 456 211 393
Cuts at: 3930 4192 4538 4757 5510 6894 7162 1632
Size: 262 346 219 753 1384 268 1917
Fragments arranged by size:
1917 1384 753 510 456 393 346 282
268 262 255 219 211 147 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7250
AflIII A'CryG_T
Cuts at: 1203 2905 6346 7323 1203
Size: 1702 3441 977 1327
Fragments arranged by size:
3441 1702 1327 977
AgeI A'CCGG_T
Cuts at: 6278 6278
Size: 7447
AhdI GACnn_n'nnGTC
Cuts at: 3773 6176 3773
Size: 2403 5044
AleI CACnn'nnGTG
Cuts at: 1166 4130 1166
Size: 2964 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3528 3747 1184
Size: 2344 219 4884
Fragments arranged by size:
4884 2344 219
AlwNI CAG_nnn'CTG
Cuts at: 2717 2757 3544 4613 5190 6762 2717
Size: 40 787 1069 577 1572 3402
Fragments arranged by size:
3402 1572 1069 787 577 40
ApaLI G'TGCA_C
Cuts at: 4000 5504 6660 4000
Size: 1504 1156 4787
Fragments arranged by size:
4787 1504 1156
ApoI r'AATT_y
Cuts at: 1493 4135 4339 4405 4627 4756 4982 5134 7243
Size: 2642 204 66 222 129 226 152 2109
Cuts at: 7243 1493
Size: 1697
Fragments arranged by size:
2642 2109 1697 226 222 204 152 129
66
AseI AT'TA_AT
Cuts at: 160 1032 6001 160
Size: 872 4969 1606
Fragments arranged by size:
4969 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
AvaI C'yCGr_G
Cuts at: 1870 1943 3803 4386 4772 1870
Size: 73 1860 583 386 4545
Fragments arranged by size:
4545 1860 583 386 73
BamHI G'GATC_C
Cuts at: 4777 5144 4777
Size: 367 7080
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4556 4768 6223 618
Size: 325 217 627 2769 212 1455 1842
Fragments arranged by size:
2769 1842 1455 627 325 217 212
BanII G_rGCy'C
Cuts at: 1391 3407 4766 1391
Size: 2016 1359 4072
Fragments arranged by size:
4072 2016 1359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3332 4664 928
Size: 971 1433 1332 3711
Fragments arranged by size:
3711 1433 1332 971
BbvCI CC'TCA_GC
Cuts at: 3635 3635
Size: 7447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5638 5672 1903
Size: 34 3701 34 3678
Fragments arranged by size:
3701 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5339 6549 5339
Size: 1210 6237
BclI T'GATC_A
Cuts at: 1548 2479 1548
Size: 931 6516
BfrBI ATG'CAT
Cuts at: 2240 2240
Size: 7447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6058 136
Size: 107 122 71 631 605 4386 1525
Fragments arranged by size:
4386 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3062 7442 3062
Size: 4380 3067
BlpI GC'TnA_GC
Cuts at: 4864 4864
Size: 7447
Bme1580I G_kGCm'C
Cuts at: 1790 4004 4561 5508 6664 1790
Size: 2214 557 947 1156 2573
Fragments arranged by size:
2573 2214 1156 947 557
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 3610 3790
Size: 416 53 83 399 24 1706 487 180
Cuts at: 3790 4535 4697 5178 6136 442
Size: 745 162 481 958 1753
Fragments arranged by size:
1753 1706 958 745 487 481 416 399
180 162 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7447
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 4412 2875
Size: 1537 5910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2474 4280 4424 6107 1239
Size: 1002 233 1806 144 1683 2579
Fragments arranged by size:
2579 1806 1683 1002 233 144
Bpu10I CC'TnA_GC
Cuts at: 3470 3635 3470
Size: 165 7282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2601 2639 4477 5572 6437 6735 2601
Size: 38 1838 1095 865 298 3313
Fragments arranged by size:
3313 1838 1095 865 298 38
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4453 6110 882
Size: 617 2954 1657 2219
Fragments arranged by size:
2954 2219 1657 617
BsaAI yAC'GTr
Cuts at: 493 1204 5238 493
Size: 711 4034 2702
Fragments arranged by size:
4034 2702 711
BsaBI GATnn'nnATC
Cuts at: 2376 7441 2376
Size: 5065 2382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5254 5636 6271 275
Size: 53 83 186 936 3721 382 635 1451
Fragments arranged by size:
3721 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5880 6278 6552 6699 7110 5880
Size: 398 274 147 411 6217
Fragments arranged by size:
6217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2203 2233 1178
Size: 30 165 30 800 30 6392
Fragments arranged by size:
6392 800 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 3404 3863 1186
Size: 1285 933 459 4770
Fragments arranged by size:
4770 1285 933 459
BseYI C'CCAG_C
Cuts at: 3268 4070 6650 7146 3268
Size: 802 2580 496 3569
Fragments arranged by size:
3569 2580 802 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2966 3592 1163
Size: 573 1230 626 5018
Fragments arranged by size:
5018 1230 626 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4897 5658 6686 1540
Size: 275 186 2896 761 1028 2301
Fragments arranged by size:
2896 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3407 4004 5508 5593 6664 3407
Size: 597 1504 85 1071 4190
Fragments arranged by size:
4190 1504 1071 597 85
BsmI GAATG_Cn'
Cuts at: 2026 4958 5051 2026
Size: 2932 93 4422
Fragments arranged by size:
4422 2932 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2542 2542
Size: 7447
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3407 4004 4561 4766 5508 5593 6664
Size: 399 1617 597 557 205 742 85 1071
Cuts at: 6664 1391
Size: 2174
Fragments arranged by size:
2174 1617 1071 742 597 557 399 205
85
BspEI T'CCGG_A
Cuts at: 7110 7110
Size: 7447
BspHI T'CATG_A
Cuts at: 2058 3312 5336 7066 2058
Size: 1254 2024 1730 2439
Fragments arranged by size:
2439 2024 1730 1254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2946 3941 4802 844
Size: 2102 995 861 3489
Fragments arranged by size:
3489 2102 995 861
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 5334 1394
Size: 429 1432 2079 3507
Fragments arranged by size:
3507 2079 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 5942 6116 65
Size: 1183 1293 3401 174 1396
Fragments arranged by size:
3401 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6091 6278 1700
Size: 110 4281 187 2869
Fragments arranged by size:
4281 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7447
BssSI C'ACGA_G
Cuts at: 2984 5507 6519 2984
Size: 2523 1012 3912
Fragments arranged by size:
3912 2523 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 794
Size: 1452 5995
BstBI TT'CG_AA
Cuts at: 2530 6251 2530
Size: 3721 3726
BstEII G'GTnAC_C
Cuts at: 1184 4063 1184
Size: 2879 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 3101 4225 4568 3101
Size: 1124 343 5980
Fragments arranged by size:
5980 1124 343
BstYI r'GATC_y
Cuts at: 1972 2113 3062 3534 3924 4777 5144 5529 5546
Size: 141 949 472 390 853 367 385 17
Cuts at: 5546 6998 7084 7096 7442 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 949 853 472 390 385 367
346 141 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3433 3433
Size: 7447
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6895
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3449 487
Size: 683 56 2223 4485
Fragments arranged by size:
4485 2223 683 56
BtsI GCAGTG_nn'
Cuts at: 3458 3646 4963 5757 5777 3458
Size: 188 1317 794 20 5128
Fragments arranged by size:
5128 1317 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7412
DraI TTT'AAA
Cuts at: 3190 4626 4751 3190
Size: 1436 125 5886
Fragments arranged by size:
5886 1436 125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6454 809
Size: 507 5138 1802
Fragments arranged by size:
5138 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4227 4486 4894
Size: 54 1425 50 275 18 2397 259 408
Cuts at: 4894 5783 7412 8
Size: 889 1629 43
Fragments arranged by size:
2397 1629 1425 889 408 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4894 1537
Size: 275 3082 4090
Fragments arranged by size:
4090 3082 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2592 2664 2688 2816 2844 3831 3840
Size: 912 327 72 24 128 28 987 9
Cuts at: 3840 3885 4514 5377 1353
Size: 45 629 863 3423
Fragments arranged by size:
3423 987 912 863 629 327 128 72
45 28 24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6034 6408 6554 387
Size: 1064 138 4445 374 146 1280
Fragments arranged by size:
4445 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3405 3405
Size: 7447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2241 2289 2474 2571 2826 2870
Size: 393 510 99 48 185 97 255 44
Cuts at: 2870 3326 3537 3930 4192 4280 4424 4538 4757
Size: 456 211 393 262 88 144 114 219
Cuts at: 4757 5510 6107 6894 7162 1239
Size: 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 510 456 393 393
268 262 255 219 211 185 144 114
99 97 88 48 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3371 4739 3371
Size: 1368 6079
EcoO109I rG'GnC_Cy
Cuts at: 3428 3428
Size: 7447
EcoRI G'AATT_C
Cuts at: 4135 4756 5134 4135
Size: 621 378 6448
Fragments arranged by size:
6448 621 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 3526 3914 4519 2870
Size: 656 388 605 5798
Fragments arranged by size:
5798 656 605 388
FspI TGC'GCA
Cuts at: 5953 5953
Size: 7447
HaeII r_GCGC'y
Cuts at: 1678 6594 1678
Size: 4916 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2203 2235 2237
Size: 32 135 32 515 32 84 32 2
Cuts at: 2237 2269 2394 2426 3161 3193 3344 3376 3759
Size: 32 125 32 735 32 151 32 383
Cuts at: 3759 3791 6088 6120 6162 6194 1373
Size: 32 2297 32 42 32 2626
Fragments arranged by size:
2626 2297 735 515 383 151 135 125
84 42 32 32 32 32 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3482 4791 5037 669
Size: 988 281 1544 1309 246 3079
Fragments arranged by size:
3079 1544 1309 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2943 748
Size: 2195 5252
HpaI GTT'AAC
Cuts at: 5037 5037
Size: 7447
KpnI G_GTAC'C
Cuts at: 4772 4772
Size: 7447
MfeI C'AATT_G
Cuts at: 2462 5046 2462
Size: 2584 4863
MluI A'CGCG_T
Cuts at: 7323 7323
Size: 7447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 3318 4146 6561 6745 1305
Size: 1308 705 828 2415 184 2007
Fragments arranged by size:
2415 2007 1308 828 705 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4229 4488 10
Size: 54 1425 343 2397 259 2969
Fragments arranged by size:
2969 2397 1425 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 4130 5405 5764 5923 518
Size: 648 1176 1788 1275 359 159 2042
Fragments arranged by size:
2042 1788 1275 1176 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2494 2574 3131 3529 4427 4863 5540
Size: 624 264 80 557 398 898 436 677
Cuts at: 5540 6688 6933 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 624 557 436 398
264 245 80
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6895
NdeI CA'TA_TG
Cuts at: 387 3100 387
Size: 2713 4734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7447
NotI GC'GGCC_GC
Cuts at: 4894 4894
Size: 7447
NsiI A_TGCA'T
Cuts at: 2242 2242
Size: 7447
NspI r_CATG'y
Cuts at: 1132 1229 2240 2909 4805 6350 1132
Size: 97 1011 669 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 1011 669 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7447
PciI A'CATG_T
Cuts at: 2905 6346 2905
Size: 3441 4006
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3415 3544 1108
Size: 2307 129 5011
Fragments arranged by size:
5011 2307 129
PfoI T'CCnGG_A
Cuts at: 4440 4466 4440
Size: 26 7421
PmeI GTTT'AAAC
Cuts at: 4751 4751
Size: 7447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5516 5548 7029 7061 3491
Size: 32 1993 32 1481 32 3877
Fragments arranged by size:
3877 1993 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3428 3428
Size: 7447
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7447
PsiI TTA'TAA
Cuts at: 5017 5017
Size: 7447
PspXI vC'TCGA_Gb
Cuts at: 4386 4386
Size: 7447
PstI C_TGCA'G
Cuts at: 830 2499 2631 4799 830
Size: 1669 132 2168 3478
Fragments arranged by size:
3478 2168 1669 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7447
PvuII CAG'CTG
Cuts at: 2494 2574 3131 4427 2494
Size: 80 557 1296 5514
Fragments arranged by size:
5514 1296 557 80
SacI G_AGCT'C
Cuts at: 3407 3407
Size: 7447
SalI G'TCGA_C
Cuts at: 1936 3480 4789 1936
Size: 1544 1309 4594
Fragments arranged by size:
4594 1544 1309
SapI GCTCTTCn'nnn_
Cuts at: 2688 2844 2688
Size: 156 7291
SbfI CC_TGCA'GG
Cuts at: 4799 4799
Size: 7447
ScaI AGT'ACT
Cuts at: 5695 7407 5695
Size: 1712 5735
SexAI A'CCwGG_T
Cuts at: 3769 3769
Size: 7447
SfcI C'TryA_G
Cuts at: 826 1045 2495 2627 3453 4030 4153 4795 5930
Size: 219 1450 132 826 577 123 642 1135
Cuts at: 5930 6611 6802 826
Size: 681 191 1471
Fragments arranged by size:
1471 1450 1135 826 681 642 577 219
191 132 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7447
SmaI CCC'GGG
Cuts at: 1872 3805 4774 1872
Size: 1933 969 4545
Fragments arranged by size:
4545 1933 969
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2616 2654 4386 4456 5551 6452
Size: 197 926 673 38 1732 70 1095 901
Cuts at: 6452 6714 820
Size: 262 1553
Fragments arranged by size:
1732 1553 1095 926 901 673 262 197
70 38
SnaBI TAC'GTA
Cuts at: 493 5238 493
Size: 4745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7447
SphI G_CATG'C
Cuts at: 1132 2240 4805 1132
Size: 1108 2565 3774
Fragments arranged by size:
3774 2565 1108
SspI AAT'ATT
Cuts at: 5 52 4038 5371 5
Size: 47 3986 1333 2081
Fragments arranged by size:
3986 2081 1333 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7447
StyI C'CwwG_G
Cuts at: 513 1065 2691 4886 513
Size: 552 1626 2195 3074
Fragments arranged by size:
3074 2195 1626 552
SwaI ATTT'AAAT
Cuts at: 4626 4626
Size: 7447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3952 4718 4735 5474 5491 5644
Size: 1255 2049 323 766 17 739 17 153
Cuts at: 5644 5829 6274 325
Size: 185 445 1498
Fragments arranged by size:
2049 1498 1255 766 739 445 323 185
153 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5693 7405 96
Size: 274 80 33 51 1443 3716 1712 138
Fragments arranged by size:
3716 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7407 7439 7407
Size: 32 7415
Tth111I GACn'n_nGTC
Cuts at: 1316 2215 1316
Size: 899 6548
XbaI T'CTAG_A
Cuts at: 4783 4783
Size: 7447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4448 4448
Size: 7447
XhoI C'TCGA_G
Cuts at: 1943 4386 1943
Size: 2443 5004
XmaI C'CCGG_G
Cuts at: 1870 3803 4772 1870
Size: 1933 969 4545
Fragments arranged by size:
4545 1933 969
XmnI GAAnn'nnTTC
Cuts at: 3506 5576 3506
Size: 2070 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5255 276
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SfiI SfoI SrfI