Restriction map for
pFN21AB8474
(Circular) (Six-base) MAPSORT of: pFN21AB8474.seq Check: 9224 from: 1 to: 7228
pFN21AB8474 7228 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5038 278
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3202 3370 4571 1937
Size: 1265 168 1201 4594
Fragments arranged by size:
4594 1265 1201 168
Acc65I G'GTAC_C
Cuts at: 4549 4549
Size: 7228
AclI AA'CG_TT
Cuts at: 1563 5355 5728 1563
Size: 3792 373 3063
Fragments arranged by size:
3792 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 3698 3773 3791 3997 5291 6675 6943
Size: 743 1323 75 18 206 1294 1384 268
Cuts at: 6943 1632
Size: 1917
Fragments arranged by size:
1917 1384 1323 1294 743 268 206 75
18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7031
AflIII A'CryG_T
Cuts at: 1203 6127 7104 1203
Size: 4924 977 1327
Fragments arranged by size:
4924 1327 977
AgeI A'CCGG_T
Cuts at: 6059 6059
Size: 7228
AhdI GACnn_n'nnGTC
Cuts at: 2135 5957 2135
Size: 3822 3406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3684 1184
Size: 2500 4728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2141
Size: 32 7196
AlwNI CAG_nnn'CTG
Cuts at: 2056 2353 2845 4147 4971 6543 2056
Size: 297 492 1302 824 1572 2741
Fragments arranged by size:
2741 1572 1302 824 492 297
ApaI G_GGCC'C
Cuts at: 2397 2540 2941 2397
Size: 143 401 6684
Fragments arranged by size:
6684 401 143
ApaLI G'TGCA_C
Cuts at: 5285 6441 5285
Size: 1156 6072
ApoI r'AATT_y
Cuts at: 1493 4537 4763 4915 7024 1493
Size: 3044 226 152 2109 1697
Fragments arranged by size:
3044 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5782 160
Size: 872 4750 1606
Fragments arranged by size:
4750 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
AvaI C'yCGr_G
Cuts at: 1870 1943 3719 4553 1870
Size: 73 1776 834 4545
Fragments arranged by size:
4545 1776 834 73
BamHI G'GATC_C
Cuts at: 2524 3807 4558 4925 2524
Size: 1283 751 367 4827
Fragments arranged by size:
4827 1283 751 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4549 6004 618
Size: 325 217 627 2762 1455 1842
Fragments arranged by size:
2762 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2397 2540 2651 2941 3423 3465 4049 4137
Size: 1006 143 111 290 482 42 584 88
Cuts at: 4137 4547 1391
Size: 410 4072
Fragments arranged by size:
4072 1006 584 482 410 290 143 111
88 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 3444 3761 928
Size: 971 218 1327 317 4395
Fragments arranged by size:
4395 1327 971 317 218
BbvCI CC'TCA_GC
Cuts at: 2417 2417
Size: 7228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5419 5453 1903
Size: 34 3482 34 3678
Fragments arranged by size:
3678 3482 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2965 5120 6330 2965
Size: 2155 1210 3863
Fragments arranged by size:
3863 2155 1210
BclI T'GATC_A
Cuts at: 1548 2812 1548
Size: 1264 5964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2828 3933 4099
Size: 107 122 71 631 605 1156 1105 166
Cuts at: 4099 5839 136
Size: 1740 1525
Fragments arranged by size:
1740 1525 1156 1105 631 605 166 122
107 71
BglII A'GATC_T
Cuts at: 4026 7223 4026
Size: 3197 4031
BlpI GC'TnA_GC
Cuts at: 2049 2691 4131 4472 4645 2049
Size: 642 1440 341 173 4632
Fragments arranged by size:
4632 1440 642 341 173
Bme1580I G_kGCm'C
Cuts at: 1790 2397 2540 2941 3406 5289 6445 1790
Size: 607 143 401 465 1883 1156 2573
Fragments arranged by size:
2573 1883 1156 607 465 401 143
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7228
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 3105 4959
Size: 416 53 83 399 24 1401 287 1854
Cuts at: 4959 5917 442
Size: 958 1753
Fragments arranged by size:
1854 1753 1401 958 416 399 287 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3314 1056
Size: 2258 4970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3982 3982
Size: 7228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 3059 3161 3500 3527 3833 4199 4421
Size: 1115 705 102 339 27 306 366 222
Cuts at: 4421 5888 1239
Size: 1467 2579
Fragments arranged by size:
2579 1467 1115 705 366 339 306 222
102 27
Bpu10I CC'TnA_GC
Cuts at: 2199 2417 3543 3931 2199
Size: 218 1126 388 5496
Fragments arranged by size:
5496 1126 388 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3037 3629 3821 3830 3959 4068 5353 6218
Size: 158 592 192 9 129 109 1285 865
Cuts at: 6218 6516 2879
Size: 298 3591
Fragments arranged by size:
3591 1285 865 592 298 192 158 129
109 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 4332 5891 882
Size: 617 2751 82 1559 2219
Fragments arranged by size:
2751 2219 1559 617 82
BsaAI yAC'GTr
Cuts at: 493 1204 5019 493
Size: 711 3815 2702
Fragments arranged by size:
3815 2702 711
BsaBI GATnn'nnATC
Cuts at: 7222 7222
Size: 7228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5035 5417 6052 275
Size: 53 83 186 936 3502 382 635 1451
Fragments arranged by size:
3502 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2064 5661 6059 6333 6480 6891 2064
Size: 3597 398 274 147 411 2401
Fragments arranged by size:
3597 2401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2364 2394 2744 2774 3152
Size: 30 165 30 961 30 350 30 378
Cuts at: 3152 3182 1178
Size: 30 5224
Fragments arranged by size:
5224 961 378 350 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2276 2621 3470 3473 3497 3704
Size: 853 3 234 345 849 3 24 207
Cuts at: 3704 3707 3749 3956 3997 4196 4418 1186
Size: 3 42 207 41 199 222 3996
Fragments arranged by size:
3996 853 849 345 234 222 207 207
199 42 41 24 3 3 3
BseYI C'CCAG_C
Cuts at: 2390 3171 3339 3355 3391 4089 4321 6431 6927
Size: 781 168 16 36 698 232 2110 496
Cuts at: 6927 2390
Size: 2691
Fragments arranged by size:
2691 2110 781 698 496 232 168 36
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2988 1163
Size: 573 498 754 5403
Fragments arranged by size:
5403 754 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4678 5439 6467 1540
Size: 275 186 2677 761 1028 2301
Fragments arranged by size:
2677 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2651 3423 3465 4049 4137 5289 5374 6445 2651
Size: 772 42 584 88 1152 85 1071 3434
Fragments arranged by size:
3434 1152 1071 772 584 88 85 42
BsmI GAATG_Cn'
Cuts at: 4739 4832 4739
Size: 93 7135
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2540 2651 2941 3406 3423 3465
Size: 399 607 143 111 290 465 17 42
Cuts at: 3465 4049 4137 4547 5289 5374 6445 1391
Size: 584 88 410 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 584 465 410 399
290 143 111 88 85 42 17
BspEI T'CCGG_A
Cuts at: 2064 6891 2064
Size: 4827 2401
BspHI T'CATG_A
Cuts at: 5117 6847 5117
Size: 1730 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4583 844
Size: 3739 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3739 3868 3930 5115 1394
Size: 429 1916 129 62 1185 3507
Fragments arranged by size:
3507 1916 1185 429 129 62
BsrDI GCAATG_nn'
Cuts at: 65 1248 3321 5723 5897 65
Size: 1183 2073 2402 174 1396
Fragments arranged by size:
2402 2073 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5872 6059 1700
Size: 110 4062 187 2869
Fragments arranged by size:
4062 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7228
BssSI C'ACGA_G
Cuts at: 2399 5288 6300 2399
Size: 2889 1012 3327
Fragments arranged by size:
3327 2889 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2313 2381 4396 4482 794
Size: 1519 68 2015 86 3540
Fragments arranged by size:
3540 2015 1519 86 68
BstBI TT'CG_AA
Cuts at: 6032 6032
Size: 7228
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7228
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2569 3100 2554
Size: 15 531 6682
Fragments arranged by size:
6682 531 15
BstYI r'GATC_y
Cuts at: 1972 2524 3045 3474 3807 4026 4558 4925 5310
Size: 552 521 429 333 219 532 367 385
Cuts at: 5310 5327 6779 6865 6877 7223 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 552 532 521 429 385 367
346 333 219 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3466 3555 4218 513
Size: 552 939 1462 89 663 3523
Fragments arranged by size:
3523 1462 939 663 552 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6489
Fragments arranged by size:
6489 683 56
BtsI GCAGTG_nn'
Cuts at: 3656 3832 4216 4744 5538 5558 3656
Size: 176 384 528 794 20 5326
Fragments arranged by size:
5326 794 528 384 176 20
ClaI AT'CG_AT
Cuts at: 1554 4281 1554
Size: 2727 4501
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7193
DraI TTT'AAA
Cuts at: 4532 4532
Size: 7228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6235 809
Size: 507 4919 1802
Fragments arranged by size:
4919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 4100 4675
Size: 54 1425 50 275 18 465 1805 575
Cuts at: 4675 5564 7193 8
Size: 889 1629 43
Fragments arranged by size:
1805 1629 1425 889 575 465 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4675 1537
Size: 275 2863 4090
Fragments arranged by size:
4090 2863 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 3269 3345 4235 5158 1353
Size: 1253 663 76 890 923 3423
Fragments arranged by size:
3423 1253 923 890 663 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5815 6189 6335 387
Size: 1064 138 4226 374 146 1280
Fragments arranged by size:
4226 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2649 3421 3463 4047 4135 2649
Size: 772 42 584 88 5742
Fragments arranged by size:
5742 772 584 88 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2375 3059 3161 3500 3527 3698
Size: 393 722 21 684 102 339 27 171
Cuts at: 3698 3773 3791 3833 3997 4199 4421 5291 5888
Size: 75 18 42 164 202 222 870 597
Cuts at: 5888 6675 6943 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 722 684 597 393 339
268 222 202 171 164 102 75 42
27 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3773 3773
Size: 7228
EcoO109I rG'GnC_Cy
Cuts at: 2394 2536 2938 3139 3841 4065 4228 4490 2394
Size: 142 402 201 702 224 163 262 5132
Fragments arranged by size:
5132 702 402 262 224 201 163 142
EcoRI G'AATT_C
Cuts at: 4537 4915 4537
Size: 378 6850
FspI TGC'GCA
Cuts at: 5734 5734
Size: 7228
HaeII r_GCGC'y
Cuts at: 1678 2914 3589 4248 6375 1678
Size: 1236 675 659 2127 2531
Fragments arranged by size:
2531 2127 1236 675 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2121 2153 2939
Size: 32 135 32 460 32 57 32 786
Cuts at: 2939 2971 3152 3184 5869 5901 5943 5975 1373
Size: 32 181 32 2685 32 42 32 2626
Fragments arranged by size:
2685 2626 786 460 181 135 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3203 3249 4572 4818 669
Size: 988 281 1265 46 1323 246 3079
Fragments arranged by size:
3079 1323 1265 988 281 246 46
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7228
HpaI GTT'AAC
Cuts at: 4818 4818
Size: 7228
KpnI G_GTAC'C
Cuts at: 4553 4553
Size: 7228
MfeI C'AATT_G
Cuts at: 4827 4827
Size: 7228
MluI A'CGCG_T
Cuts at: 7104 7104
Size: 7228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6342 6526 1305
Size: 5037 184 2007
Fragments arranged by size:
5037 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5406
Fragments arranged by size:
5406 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 5186 5545 5704 518
Size: 648 1125 2895 359 159 2042
Fragments arranged by size:
2895 2042 1125 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2332 2504 2632 2899 3107 3856 4144
Size: 432 294 172 128 267 208 749 288
Cuts at: 4144 4317 4644 5321 6469 6714 1606
Size: 173 327 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 432 327 294 288
267 245 208 173 172 128
NcoI C'CATG_G
Cuts at: 513 1065 2004 3555 4218 513
Size: 552 939 1551 663 3523
Fragments arranged by size:
3523 1551 939 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7228
NheI G'CTAG_C
Cuts at: 1052 3310 1052
Size: 2258 4970
NotI GC'GGCC_GC
Cuts at: 4675 4675
Size: 7228
NspI r_CATG'y
Cuts at: 1132 1229 2924 4399 4586 6131 1132
Size: 97 1695 1475 187 1545 2229
Fragments arranged by size:
2229 1695 1545 1475 187 97
PasI CC'CwG_GG
Cuts at: 1348 2531 1348
Size: 1183 6045
PciI A'CATG_T
Cuts at: 6127 6127
Size: 7228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3573 1108
Size: 2465 4763
PfoI T'CCnGG_A
Cuts at: 2055 3081 3177 3477 3810 2055
Size: 1026 96 300 333 5473
Fragments arranged by size:
5473 1026 333 300 96
PmeI GTTT'AAAC
Cuts at: 4532 4532
Size: 7228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5297 5329 6810 6842 5297
Size: 32 1481 32 5683
Fragments arranged by size:
5683 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3139 3841 4065 4228 3139
Size: 702 224 163 6139
Fragments arranged by size:
6139 702 224 163
PshAI GACnn'nnGTC
Cuts at: 1613 4262 1613
Size: 2649 4579
PsiI TTA'TAA
Cuts at: 4798 4798
Size: 7228
PspOMI G'GGCC_C
Cuts at: 2393 2536 2937 2393
Size: 143 401 6684
Fragments arranged by size:
6684 401 143
PspXI vC'TCGA_Gb
Cuts at: 3719 3719
Size: 7228
PstI C_TGCA'G
Cuts at: 830 2241 2318 2371 2469 2679 2742 2850 3615
Size: 1411 77 53 98 210 63 108 765
Cuts at: 3615 3891 3903 4392 4580 830
Size: 276 12 489 188 3478
Fragments arranged by size:
3478 1411 765 489 276 210 188 108
98 77 63 53 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7228
PvuII CAG'CTG
Cuts at: 2332 2504 2632 3107 3856 2332
Size: 172 128 475 749 5704
Fragments arranged by size:
5704 749 475 172 128
SacI G_AGCT'C
Cuts at: 2651 3423 3465 4049 4137 2651
Size: 772 42 584 88 5742
Fragments arranged by size:
5742 772 584 88 42
SalI G'TCGA_C
Cuts at: 1936 3201 4570 1936
Size: 1265 1369 4594
Fragments arranged by size:
4594 1369 1265
SapI GCTCTTCn'nnn_
Cuts at: 3345 4235 3345
Size: 890 6338
SbfI CC_TGCA'GG
Cuts at: 2469 4580 2469
Size: 2111 5117
ScaI AGT'ACT
Cuts at: 5476 7188 5476
Size: 1712 5516
SfcI C'TryA_G
Cuts at: 826 1045 2237 2314 2367 2423 2465 2675 2738
Size: 219 1192 77 53 56 42 210 63
Cuts at: 2738 2846 3611 3698 3887 3899 4388 4576 5711
Size: 108 765 87 189 12 489 188 1135
Cuts at: 5711 6392 6583 826
Size: 681 191 1471
Fragments arranged by size:
1471 1192 1135 765 681 489 219 210
191 189 188 108 87 77 63 56
53 42 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7228
SmaI CCC'GGG
Cuts at: 1872 4555 1872
Size: 2683 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3016 3644 3719 3836 3845
Size: 197 926 915 158 628 75 117 9
Cuts at: 3845 3938 4083 5332 6233 6495 820
Size: 93 145 1249 901 262 1553
Fragments arranged by size:
1553 1249 926 915 901 628 262 197
158 145 117 93 75 9
SnaBI TAC'GTA
Cuts at: 493 5019 493
Size: 4526 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7228
SphI G_CATG'C
Cuts at: 1132 4586 1132
Size: 3454 3774
SspI AAT'ATT
Cuts at: 5 52 5152 5
Size: 47 5100 2081
Fragments arranged by size:
5100 2081 47
StuI AGG'CCT
Cuts at: 1517 3936 1517
Size: 2419 4809
StyI C'CwwG_G
Cuts at: 513 1065 2004 3555 3567 4218 4667 513
Size: 552 939 1551 12 651 449 3074
Fragments arranged by size:
3074 1551 939 651 552 449 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5255 5272 5425 5610 6055 325
Size: 1255 3675 17 153 185 445 1498
Fragments arranged by size:
3675 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5474 7186 96
Size: 274 80 33 51 1443 3497 1712 138
Fragments arranged by size:
3497 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7188 7220 7188
Size: 32 7196
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7228
XbaI T'CTAG_A
Cuts at: 4564 4564
Size: 7228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 2875
Size: 7228
XhoI C'TCGA_G
Cuts at: 1943 3719 1943
Size: 1776 5452
XmaI C'CCGG_G
Cuts at: 1870 4553 1870
Size: 2683 4545
XmnI GAAnn'nnTTC
Cuts at: 3216 3534 5357 3216
Size: 318 1823 5087
Fragments arranged by size:
5087 1823 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5036 276
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI