Restriction map for
pFN21AB8470
(Circular) (Six-base) MAPSORT of: pFN21AB8470.seq Check: 7230 from: 1 to: 7171
pFN21AB8470 7171 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2657 2657
Size: 7171
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3270 4212 4981 278
Size: 53 83 186 936 1734 942 769 2468
Fragments arranged by size:
2468 1734 942 936 769 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3999 4452 4514 1937
Size: 2062 453 62 4594
Fragments arranged by size:
4594 2062 453 62
Acc65I G'GTAC_C
Cuts at: 4266 4492 4266
Size: 226 6945
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3746 4409 5234 6618 6886 1632
Size: 1301 813 663 825 1384 268 1917
Fragments arranged by size:
1917 1384 1301 825 813 663 268
AfeI AGC'GCT
Cuts at: 2946 2946
Size: 7171
AflII C'TTAA_G
Cuts at: 820 1017 2752 3365 820
Size: 197 1735 613 4626
Fragments arranged by size:
4626 1735 613 197
AflIII A'CryG_T
Cuts at: 1203 3007 4104 6070 7047 1203
Size: 1804 1097 1966 977 1327
Fragments arranged by size:
1966 1804 1327 1097 977
AgeI A'CCGG_T
Cuts at: 3281 6002 3281
Size: 2721 4450
AhdI GACnn_n'nnGTC
Cuts at: 5900 5900
Size: 7171
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7171
AlwNI CAG_nnn'CTG
Cuts at: 3503 3964 4047 4914 6486 3503
Size: 461 83 867 1572 4188
Fragments arranged by size:
4188 1572 867 461 83
ApaLI G'TGCA_C
Cuts at: 4022 5228 6384 4022
Size: 1206 1156 4809
Fragments arranged by size:
4809 1206 1156
ApoI r'AATT_y
Cuts at: 1493 2214 2315 4190 4480 4706 4858 6967 1493
Size: 721 101 1875 290 226 152 2109 1697
Fragments arranged by size:
2109 1875 1697 721 290 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4098 4496 1870
Size: 73 2155 398 4545
Fragments arranged by size:
4545 2155 398 73
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4266 4492 5947 618
Size: 325 217 627 2479 226 1455 1842
Fragments arranged by size:
2479 1842 1455 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 2750 3777 4386 4490 1391
Size: 1359 1027 609 104 4072
Fragments arranged by size:
4072 1359 1027 609 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3725 928
Size: 971 1826 4374
Fragments arranged by size:
4374 1826 971
BbvCI CC'TCA_GC
Cuts at: 3924 3924
Size: 7171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5362 5396 1903
Size: 34 3425 34 3678
Fragments arranged by size:
3678 3425 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5782 136
Size: 107 122 71 631 605 4110 1525
Fragments arranged by size:
4110 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2793 3702 7166 2793
Size: 909 3464 2798
Fragments arranged by size:
3464 2798 909
BlpI GC'TnA_GC
Cuts at: 4588 4588
Size: 7171
Bme1580I G_kGCm'C
Cuts at: 1790 3898 4026 4340 5232 6388 1790
Size: 2108 128 314 892 1156 2573
Fragments arranged by size:
2573 2108 1156 892 314 128
BmgBI CAC'GTC
Cuts at: 1318 2106 4105 1318
Size: 788 1999 4384
Fragments arranged by size:
4384 1999 788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3541 4902 5860
Size: 416 53 83 399 24 2124 1361 958
Cuts at: 5860 442
Size: 1753
Fragments arranged by size:
2124 1753 1361 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3489 3489
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 3026 3773 4152 4234 5831 1239
Size: 862 925 747 379 82 1597 2579
Fragments arranged by size:
2579 1597 925 862 747 379 82
Bpu10I CC'TnA_GC
Cuts at: 3690 3924 3690
Size: 234 6937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3560 5296 6161 6459 3560
Size: 1736 865 298 4272
Fragments arranged by size:
4272 1736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5834 882
Size: 617 4335 2219
Fragments arranged by size:
4335 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4335 4962 493
Size: 711 3131 627 2702
Fragments arranged by size:
3131 2702 711 627
BsaBI GATnn'nnATC
Cuts at: 7165 7165
Size: 7171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3267 3819 4007 4209
Size: 53 83 186 936 1734 552 188 202
Cuts at: 4209 4978 5360 5995 275
Size: 769 382 635 1451
Fragments arranged by size:
1734 1451 936 769 635 552 382 202
188 186 83 53
BsaWI w'CCGG_w
Cuts at: 2674 3281 4447 5604 6002 6276 6423 6834 2674
Size: 607 1166 1157 398 274 147 411 3011
Fragments arranged by size:
3011 1166 1157 607 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6946
Fragments arranged by size:
6946 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7171
BseYI C'CCAG_C
Cuts at: 3403 3975 4011 4363 6374 6870 3403
Size: 572 36 352 2011 496 3704
Fragments arranged by size:
3704 2011 572 496 352 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2158 1163
Size: 573 422 6176
Fragments arranged by size:
6176 573 422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4621 5382 6410 1540
Size: 275 186 1285 1335 761 1028 2301
Fragments arranged by size:
2301 1335 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3777 3801 4026 5232 5317 6388 3777
Size: 24 225 1206 85 1071 4560
Fragments arranged by size:
4560 1206 1071 225 85 24
BsmI GAATG_Cn'
Cuts at: 2910 3677 4682 4775 2910
Size: 767 1005 93 5306
Fragments arranged by size:
5306 1005 767 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 3777 3801 3898 4026 4340 4386
Size: 399 960 1027 24 97 128 314 46
Cuts at: 4386 4490 5232 5317 6388 1391
Size: 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 1027 960 742 399 314 128
104 97 85 46 24
BspEI T'CCGG_A
Cuts at: 6834 6834
Size: 7171
BspHI T'CATG_A
Cuts at: 5060 6790 5060
Size: 1730 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2577 2657 4526 844
Size: 1517 216 80 1869 3489
Fragments arranged by size:
3489 1869 1517 216 80
BsrBI CCG'CTC
Cuts at: 1394 1823 5058 1394
Size: 429 3235 3507
Fragments arranged by size:
3507 3235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3427 5666 5840 65
Size: 1183 2179 2239 174 1396
Fragments arranged by size:
2239 2179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3281 5815 6002 1700
Size: 110 1471 2534 187 2869
Fragments arranged by size:
2869 2534 1471 187 110
BsrGI T'GTAC_A
Cuts at: 96 4107 4182 96
Size: 4011 75 3085
Fragments arranged by size:
4011 3085 75
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3964 4021 794
Size: 3170 57 3944
Fragments arranged by size:
3944 3170 57
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 2702 1184
Size: 1518 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 3554 3554
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2130 2194 2793 3525 3702 4501 4868 5253
Size: 158 64 599 732 177 799 367 385
Cuts at: 5253 5270 6722 6808 6820 7166 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 799 732 599 385 367 346
177 158 86 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 3890 3890
Size: 7171
BtgI C'CryG_G
Cuts at: 513 1065 2037 3031 3939 3987 513
Size: 552 972 994 908 48 3697
Fragments arranged by size:
3697 994 972 908 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 487
Size: 683 56 980 5452
Fragments arranged by size:
5452 980 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 3720 4687 5481 5501 2125
Size: 1595 967 794 20 3795
Fragments arranged by size:
3795 1595 967 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 4475 4475
Size: 7171
DraIII CAC_nnn'GTG
Cuts at: 4021 4021
Size: 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2392 6178 809
Size: 507 1076 3786 1802
Fragments arranged by size:
3786 1802 1076 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2607 4035 4135
Size: 54 1425 50 275 18 777 1428 100
Cuts at: 4135 4434 4618 5507 7136 8
Size: 299 184 889 1629 43
Fragments arranged by size:
1629 1428 1425 889 777 299 275 184
100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5101 1353
Size: 3748 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2822 5758 6132 6278 387
Size: 1064 138 1233 2936 374 146 1280
Fragments arranged by size:
2936 1280 1233 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3775 3775
Size: 7171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2933 3026 3746 3773 4152 4234
Size: 393 469 832 93 720 27 379 82
Cuts at: 4234 4409 5234 5831 6618 6886 1239
Size: 175 825 597 787 268 1524
Fragments arranged by size:
1524 832 825 787 720 597 469 393
379 268 175 93 82 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2017 2017
Size: 7171
EcoO109I rG'GnC_Cy
Cuts at: 3874 3874
Size: 7171
EcoRI G'AATT_C
Cuts at: 4480 4858 4480
Size: 378 6793
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 2948 6318 1678
Size: 1270 3370 2531
Fragments arranged by size:
3370 2531 1270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2486 2502 2518 3115
Size: 32 135 32 898 16 16 16 597
Cuts at: 3115 3147 3416 3448 5812 5844 5886 5918 1373
Size: 32 269 32 2364 32 42 32 2626
Fragments arranged by size:
2626 2364 898 597 269 135 42 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2842 4515 4761 669
Size: 988 281 904 1673 246 3079
Fragments arranged by size:
3079 1673 988 904 281 246
HindIII A'AGCT_T
Cuts at: 748 2268 748
Size: 1520 5651
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 4270 4496 4270
Size: 226 6945
MfeI C'AATT_G
Cuts at: 2295 2581 4770 2295
Size: 286 2189 4696
Fragments arranged by size:
4696 2189 286
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2912 6285 6469 1305
Size: 1607 3373 184 2007
Fragments arranged by size:
3373 2007 1607 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4037 4137 4436 10
Size: 54 1425 343 2205 100 299 2745
Fragments arranged by size:
2745 2205 1425 343 299 100 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2455 2724 3062 5129 5488 5647 518
Size: 648 1289 269 338 2067 359 159 2042
Fragments arranged by size:
2067 2042 1289 648 359 338 269 159
MspA1I CmG'CkG
Cuts at: 1606 3046 3989 4273 4587 5264 6412 6657 1606
Size: 1440 943 284 314 677 1148 245 2120
Fragments arranged by size:
2120 1440 1148 943 677 314 284 245
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 5647
Fragments arranged by size:
5647 972 552
NdeI CA'TA_TG
Cuts at: 387 2721 387
Size: 2334 4837
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2460 3011 4529 6074 1132
Size: 97 1231 551 1518 1545 2229
Fragments arranged by size:
2229 1545 1518 1231 551 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7171
PciI A'CATG_T
Cuts at: 3007 6070 3007
Size: 3063 4108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4158 1108
Size: 3050 4121
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 7171
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PmlI CAC'GTG
Cuts at: 4335 4335
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3874 3874
Size: 7171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PstI C_TGCA'G
Cuts at: 830 2358 2574 3214 4323 4523 830
Size: 1528 216 640 1109 200 3478
Fragments arranged by size:
3478 1528 1109 640 216 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 3046 4273 3046
Size: 1227 5944
SacI G_AGCT'C
Cuts at: 3777 3777
Size: 7171
SacII CC_GC'GG
Cuts at: 3990 3990
Size: 7171
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 3551 5419 7131 3551
Size: 1868 1712 3591
Fragments arranged by size:
3591 1868 1712
SexAI A'CCwGG_T
Cuts at: 2706 2706
Size: 7171
SfcI C'TryA_G
Cuts at: 826 1045 2354 2570 2656 3210 4123 4319 4519
Size: 219 1309 216 86 554 913 196 200
Cuts at: 4519 5654 6335 6526 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 913 681 554 219 216
200 196 191 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4100 4498 1872
Size: 2228 398 4545
Fragments arranged by size:
4545 2228 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3365 3575 5275 6176 6438
Size: 197 926 809 613 210 1700 901 262
Cuts at: 6438 820
Size: 1553
Fragments arranged by size:
1700 1553 926 901 809 613 262 210
197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7171
SphI G_CATG'C
Cuts at: 1132 4529 1132
Size: 3397 3774
SspI AAT'ATT
Cuts at: 5 52 2554 2973 5095 5
Size: 47 2502 419 2122 2081
Fragments arranged by size:
2502 2122 2081 419 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2037 4437 4610 513
Size: 552 972 2400 173 3074
Fragments arranged by size:
3074 2400 972 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3289 5198 5215 5368 5553 5998 325
Size: 1255 1709 1909 17 153 185 445 1498
Fragments arranged by size:
1909 1709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 2996 3549
Size: 274 80 33 51 1443 549 470 553
Cuts at: 3549 3858 4107 4182 5417 7129 96
Size: 309 249 75 1235 1712 138
Fragments arranged by size:
1712 1443 1235 553 549 470 309 274
249 138 80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7131 7163 7131
Size: 32 7139
Tth111I GACn'n_nGTC
Cuts at: 1316 3952 1316
Size: 2636 4535
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2949 4048 2949
Size: 1099 6072
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 4098 4496 1870
Size: 2228 398 4545
Fragments arranged by size:
4545 2228 398
XmnI GAAnn'nnTTC
Cuts at: 2405 5300 2405
Size: 2895 4276
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3268 4210 4979 276
Size: 53 83 186 936 1734 942 769 2468
Fragments arranged by size:
2468 1734 942 936 769 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SanDI SapI SfiI SfoI SrfI SwaI