Restriction map for
pFN21AB8463
(Circular) (Six-base) MAPSORT of: pFN21AB8463.seq Check: 2920 from: 1 to: 5749
pFN21AB8463 5749 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 2470
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2700 3559 278
Size: 53 83 186 936 1164 859 2468
Fragments arranged by size:
2468 1164 936 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2252 2609 2811 3812 5196 5464 1632
Size: 485 135 357 202 1001 1384 268 1917
Fragments arranged by size:
1917 1384 1001 485 357 268 202 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 2270 1166
Size: 1104 4645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2565 2594 2945 1184
Size: 1381 29 351 3988
Fragments arranged by size:
3988 1381 351 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 5717
AlwNI CAG_nnn'CTG
Cuts at: 3492 5064 3492
Size: 1572 4177
ApaI G_GGCC'C
Cuts at: 2086 2431 2086
Size: 345 5404
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2664 2912 3074 1870
Size: 73 231 490 248 162 4545
Fragments arranged by size:
4545 490 248 231 162 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2416 2762 3070 4525 618
Size: 325 217 627 629 346 308 1455 1842
Fragments arranged by size:
1842 1455 629 627 346 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2086 2431 3068 1391
Size: 695 345 637 4072
Fragments arranged by size:
4072 695 637 345
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BbvCI CC'TCA_GC
Cuts at: 2603 2603
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 2784 1548
Size: 1236 4513
BfrBI ATG'CAT
Cuts at: 2167 2167
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2558 2813
Size: 107 122 71 631 605 475 411 255
Cuts at: 2813 2885 4360 136
Size: 72 1475 1525
Fragments arranged by size:
1525 1475 631 605 475 411 255 122
107 72 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2431 2767 2946 3810 4966 1790
Size: 296 345 336 179 864 1156 2573
Fragments arranged by size:
2573 1156 864 345 336 296 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 2726 3480
Size: 416 53 83 399 24 946 363 754
Cuts at: 3480 4438 442
Size: 958 1753
Fragments arranged by size:
1753 958 946 754 416 399 363 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2831 2858 4409 1239
Size: 869 723 27 1551 2579
Fragments arranged by size:
2579 1551 869 723 27
Bpu10I CC'TnA_GC
Cuts at: 2603 2603
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2970 3874 4739 5037 2970
Size: 904 865 298 3682
Fragments arranged by size:
3682 904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2941 3540 493
Size: 711 1737 599 2702
Fragments arranged by size:
2702 1737 711 599
BsaBI GATnn'nnATC
Cuts at: 3023 5743 3023
Size: 2720 3029
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2697 3556 3938 4573
Size: 53 83 186 936 1164 859 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1164 936 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2486 2516 1178
Size: 30 165 30 693 30 360 30 4411
Fragments arranged by size:
4411 693 360 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2507 2510 2513 2591 1186
Size: 931 390 3 3 78 4344
Fragments arranged by size:
4344 931 390 78 3 3
BseYI C'CCAG_C
Cuts at: 2363 4952 5448 2363
Size: 2589 496 2664
Fragments arranged by size:
2664 2589 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 1163
Size: 573 1095 4081
Fragments arranged by size:
4081 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2325 3810 3895 4966 2277
Size: 48 1485 85 1071 3060
Fragments arranged by size:
3060 1485 1071 85 48
BsmI GAATG_Cn'
Cuts at: 2881 3260 3353 2881
Size: 379 93 5277
Fragments arranged by size:
5277 379 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2277 2325 2431 2767 2946 3068
Size: 399 296 191 48 106 336 179 122
Cuts at: 3068 3810 3895 4966 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 296 191 179
122 106 85 48
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 3104 844
Size: 1626 634 3489
Fragments arranged by size:
3489 1626 634
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2578 4244 4418 65
Size: 1183 1330 1666 174 1396
Fragments arranged by size:
1666 1396 1330 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2759 4393 4580 1700
Size: 110 949 1634 187 2869
Fragments arranged by size:
2869 1634 949 187 110
BsrGI T'GTAC_A
Cuts at: 96 2388 96
Size: 2292 3457
BssHII G'CGCG_C
Cuts at: 1925 2867 1925
Size: 942 4807
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2965 2965
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2958 513
Size: 552 939 954 3304
Fragments arranged by size:
3304 954 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 3265 4059 4079 2064
Size: 1201 794 20 3734
Fragments arranged by size:
3734 1201 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2528 4756 809
Size: 507 1212 2228 1802
Fragments arranged by size:
2228 1802 1212 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2991 3196
Size: 54 1425 50 275 18 244 917 205
Cuts at: 3196 4085 5714 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 917 889 275 244 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2117 2252 2609 2811 2831 2858
Size: 393 476 9 135 357 202 20 27
Cuts at: 2858 3812 4409 5196 5464 1239
Size: 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 597 476 393 357 268
202 135 27 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2390 2543 2390
Size: 153 5596
EcoO109I rG'GnC_Cy
Cuts at: 2083 2119 2427 2560 2887 2908 2926 2083
Size: 36 308 133 327 21 18 4906
Fragments arranged by size:
4906 327 308 133 36 21 18
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 2255 4255 2255
Size: 2000 3749
FspAI rTGC'GCAy
Cuts at: 2255 2255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2610 4896 1678
Size: 932 2286 2531
Fragments arranged by size:
2531 2286 932
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 4390 4422 4464
Size: 32 135 32 524 32 2262 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 2262 524 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2223 748
Size: 1475 4274
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2076 10
Size: 54 1425 343 244 3683
Fragments arranged by size:
3683 1425 343 244 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2467 2963 3707 4066 4225 518
Size: 648 1104 197 496 744 359 159 2042
Fragments arranged by size:
2042 1104 744 648 496 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 2407 3007 3165 3842 4990 5235 1606
Size: 801 600 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 600 245 158
NcoI C'CATG_G
Cuts at: 513 1065 2004 2958 513
Size: 552 939 954 3304
Fragments arranged by size:
3304 954 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NsiI A_TGCA'T
Cuts at: 2169 2169
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2404 2744 3107 4652 1132
Size: 97 1175 340 363 1545 2229
Fragments arranged by size:
2229 1545 1175 363 340 97
PasI CC'CwG_GG
Cuts at: 1348 2563 1348
Size: 1215 4534
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 1108
Size: 1191 4558
PfoI T'CCnGG_A
Cuts at: 2505 2517 2835 2505
Size: 12 318 5419
Fragments arranged by size:
5419 318 12
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PmlI CAC'GTG
Cuts at: 2941 2941
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2486 2518 3818 3850 5331 5363 2486
Size: 32 1300 32 1481 32 2872
Fragments arranged by size:
2872 1481 1300 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2119
Size: 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2082 2427 2082
Size: 345 5404
PstI C_TGCA'G
Cuts at: 830 2112 2481 2544 2934 3101 830
Size: 1282 369 63 390 167 3478
Fragments arranged by size:
3478 1282 390 369 167 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2407 2407
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 2544 3101 2544
Size: 557 5192
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2108 2477 2540 2930 3097 4232 4913
Size: 219 1063 369 63 390 167 1135 681
Cuts at: 4913 5104 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1063 681 390 369 219 191
167 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2664 2985 3853 4754 5016 820
Size: 197 926 721 321 868 901 262 1553
Fragments arranged by size:
1553 926 901 868 721 321 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2883 2958 3188 513
Size: 552 939 21 858 75 230 3074
Fragments arranged by size:
3074 939 858 552 230 75 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 3776 3793 3946 4131 4576 325
Size: 1255 489 1707 17 153 185 445 1498
Fragments arranged by size:
1707 1498 1255 489 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 3995 5707
Size: 274 80 33 51 1443 411 1607 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1712 1607 1443 411 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 2330 1316
Size: 1014 4735
XbaI T'CTAG_A
Cuts at: 2317 3085 2317
Size: 768 4981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 2664 1943
Size: 721 5028
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2698 3557 276
Size: 53 83 186 936 1164 859 2468
Fragments arranged by size:
2468 1164 936 859 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI